Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,068,922 G→T 5.6% intergenic (‑114/+25) argO ← / ← mscS arginine transporter/mechanosensitive channel protein, small conductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,068,9220GT 5.6% 380.5 / 6.5 127intergenic (‑114/+25)argO/mscSarginine transporter/mechanosensitive channel protein, small conductance
Reads supporting (aligned to +/- strand):  ref base G (52/67);  new base T (4/4);  total (56/71)
Fisher's exact test for biased strand distribution p-value = 7.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

CCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACGTTTTGC  >  NC_000913/3068773‑3069070
                                                                                                                                                     |                                                                                                                                                    
cccAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCagag                                                                                                                                                      <  1:1048/150‑1 (MQ=255)
  cAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCagagag                                                                                                                                                    >  2:547183/1‑150 (MQ=255)
    aGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGta                                                                                                                                                  >  1:1255849/1‑150 (MQ=255)
      tGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGtatt                                                                                                                                                <  2:660831/150‑1 (MQ=255)
       gCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGtatta                                                                                                                                               >  2:413209/1‑150 (MQ=255)
       gCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGtatta                                                                                                                                               >  1:139188/1‑150 (MQ=255)
       gCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCAAGAGAGtatta                                                                                                                                               >  1:1570413/1‑150 (MQ=255)
        cAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGtattat                                                                                                                                              <  2:812981/150‑1 (MQ=255)
        cAAGACATTGAAAGTATAAGAAAACACGTTGGATATTCCGAATTAAGTAACTGATGCAGAGACTTTATCGCGGTATTGTTATTTCCGGAAATTGATAATATTAATTGGACATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGtattata                                                                                                                                             >  2:283620/1‑149 (MQ=255)
         aaGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATc                                                                                                                                             >  1:1628688/1‑150 (MQ=255)
            aCCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTgg                                                                                                                                          <  2:1685501/150‑1 (MQ=255)
             ccTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGc                                                                                                                                         >  2:170034/1‑150 (MQ=255)
             ccTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGAATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGc                                                                                                                                         <  1:473378/150‑1 (MQ=255)
             acTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGc                                                                                                                                         <  1:533522/149‑1 (MQ=255)
              cTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCTGAAATTGATAATATTAATTCGCAATCAGAAAAACAAATAAGAGAGAGGCCAGATAATACTCTCTGGcc                                                                                                                                        <  2:160755/150‑1 (MQ=255)
              cTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGcc                                                                                                                                        <  1:1509336/150‑1 (MQ=255)
               ttGAAAGTAATAAGAAAACACGTTGGATATTAATAATTAAGTATCTGATTCAGAGACTATATTGCGTGATTGTTATTAGAGTAAATTTATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGCCt                                                                                                                                       <  2:1728761/150‑1 (MQ=255)
                 gAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGCCTct                                                                                                                                     <  1:611118/150‑2 (MQ=255)
                 gAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCa                                                                                                                                     <  1:1333580/150‑1 (MQ=255)
                    aGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCg                                                                                                                                  <  2:1174975/150‑1 (MQ=255)
                      taataaGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGtt                                                                                                                                <  1:1000234/150‑1 (MQ=255)
                       aataaGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTg                                                                                                                               <  2:824427/150‑1 (MQ=255)
                         taaGAAAACACGTTGTATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGATATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATATGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGAt                                                                                                                             <  2:496537/150‑1 (MQ=255)
                           aGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCTTTGATTa                                                                                                                           >  1:820636/1‑150 (MQ=255)
                            gAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTAc                                                                                                                          >  1:782339/1‑150 (MQ=255)
                              aaaCACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGc                                                                                                                        <  1:1310788/150‑1 (MQ=255)
                              aaaCACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATATTGCCTCAGCGTTGATTaaga                                                                                                                        >  1:1014650/1‑147 (MQ=255)
                                acacGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAg                                                                                                                      <  1:572238/150‑1 (MQ=255)
                                      tGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTTATAAGAGAGAGGCCAGATAGTTTTATCTGCCCTCAGCGTTGATTACGCAGCTTTGt                                                                                                                >  1:288537/1‑150 (MQ=255)
                                          tatTCCTAATTAAGTATCTGATGCAGAGACTATATCGCGGTATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGtcttc                                                                                                            <  1:115720/150‑1 (MQ=255)
                                           atTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGtcttct                                                                                                           >  2:535655/1‑150 (MQ=255)
                                             tactaaTTCAGTATCTGATGCAGAGACTATATCGCTTGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAGTTTTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCttt                                                                                                         <  2:1286944/146‑1 (MQ=255)
                                               cGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCa                                                                                                       <  1:1296715/150‑1 (MQ=255)
                                                gAATTAAGTATCTGATGCAGATACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCAc                                                                                                      <  2:22396/150‑1 (MQ=255)
                                                 aaatAATTATCTTATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCAcc                                                                                                     <  1:1153083/147‑1 (MQ=255)
                                                   ttAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGTAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTCCGCAGCTTTGTCGTCTTTCACCCg                                                                                                   >  2:151350/1‑150 (MQ=255)
                                                   ttAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCg                                                                                                   >  1:1119302/1‑150 (MQ=255)
                                                       gTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTa                                                                                               >  1:492966/1‑150 (MQ=255)
                                                        tATCAGATTCAGACACTATATAGAGGGATTGTTATTAGCTGAAATTTATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGACAGAGAGAATTATCTCGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTaa                                                                                              <  1:236072/150‑1 (MQ=255)
                                                         aTCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTaaa                                                                                             >  2:463070/1‑150 (MQ=255)
                                                         aTCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGACAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTaaa                                                                                             >  1:982266/1‑150 (MQ=255)
                                                           cTGATTCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGt                                                                                           <  1:653259/150‑1 (MQ=255)
                                                           cTGATGCAGAGACTATATCGGGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCATAAAAACTAATAAGAGAGAGTCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGt                                                                                           <  2:1397889/150‑1 (MQ=255)
                                                           cTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATAGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGt                                                                                           <  1:1206948/150‑1 (MQ=255)
                                                            tGATGCAGAGCCTAAATCGAGGGATTGTTATTAGCGGAAATTGATAATATAAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGCGAGTATTATATGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACACGCTGAAAGtt                                                                                          >  1:1372320/1‑150 (MQ=255)
                                                              aTGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTca                                                                                        <  2:533912/150‑1 (MQ=255)
                                                              aTGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCATCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACACGCTTAAAGTTca                                                                                        >  2:1623575/1‑150 (MQ=255)
                                                                  agagACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTAAAAGTTCACATc                                                                                    >  1:1639438/1‑150 (MQ=255)
                                                                        tataTCGCGGGATTGTTATTAGCGGAAATTGCAAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTg                                                                              <  2:570380/150‑1 (MQ=255)
                                                                           atCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCgg                                                                           >  2:369881/1‑150 (MQ=255)
                                                                               cgGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGGTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTAc                                                                       <  2:1133732/150‑1 (MQ=255)
                                                                                 ggATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCTGGTACgg                                                                     <  2:850174/150‑1 (MQ=255)
                                                                                  gATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACggg                                                                    >  1:1504081/1‑150 (MQ=255)
                                                                                  gATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGACTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACACGCTTAAAGTTCACATCCATTTGCGGGTACggg                                                                    >  1:1510761/1‑150 (MQ=255)
                                                                                      gTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCAGCTTAAAGTTCACATCCATTTGCGGGTCCGGGAAGc                                                                >  2:236721/1‑150 (MQ=255)
                                                                                        tattaGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGAATTATTTCACCCGCTTAAAGTACACATCCATTTGCGGGTACGGGAAgatt                                                              >  1:1182166/1‑147 (MQ=255)
                                                                                          ttaTCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGCGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGAt                                                            <  1:1036652/150‑1 (MQ=255)
                                                                                          ttaGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGAt                                                            <  1:416410/150‑1 (MQ=255)
                                                                                             gCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATAcc                                                         <  2:689779/150‑1 (MQ=255)
                                                                                               gtaaaaTGATAATAATAATTGGCAATCAGAAAAACTAAAAATACAGAGGCCATAGAGTATTTTCTGGGCTCATCGTTGATTAGGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATATGCGGGTACGGGAAGCTTATACcgg                                                       <  2:1259815/148‑1 (MQ=255)
                                                                                               ggAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACcgg                                                       <  2:1064217/150‑1 (MQ=255)
                                                                                               ggAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACcgg                                                       <  2:1348969/150‑1 (MQ=255)
                                                                                                     tGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCAt                                                 <  1:535655/150‑1 (MQ=255)
                                                                                                      gATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATc                                                <  1:1645359/150‑1 (MQ=255)
                                                                                                             ttAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCa                                         <  1:1332818/150‑1 (MQ=255)
                                                                                                              tAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCAc                                        >  1:947198/1‑150 (MQ=255)
                                                                                                              aaaTTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCAc                                        <  2:1538103/149‑1 (MQ=255)
                                                                                                               aaTTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTTATACCGGCGGCATCAAATTCACg                                       >  2:1682641/1‑150 (MQ=255)
                                                                                                               aaTTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACg                                       <  1:1333334/150‑1 (MQ=255)
                                                                                                                 tttgCAATCATAAATACTAATAAGAGAGAGGCCAGTGAGTATTATCTGTCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCATAAAGTTAACATCAATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGtt                                     <  1:283620/147‑1 (MQ=255)
                                                                                                                 ttGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGtt                                     <  1:686569/150‑1 (MQ=255)
                                                                                                                  tGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGttt                                    <  2:492966/150‑1 (MQ=255)
                                                                                                                  tGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGttt                                    <  1:1675908/150‑1 (MQ=255)
                                                                                                                  tGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGttt                                    <  1:1542992/150‑1 (MQ=255)
                                                                                                                     cAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAAt                                 <  1:547183/150‑1 (MQ=255)
                                                                                                                       aTCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATAc                               >  1:1393764/1‑150 (MQ=255)
                                                                                                                         cAGAAAAACTAATAAGAGAGAGGCCAGCGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGGCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGGACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGc                             <  1:1066818/150‑1 (MQ=255)
                                                                                                                         cAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTACTTTCACCCGCTTAAAGTTCACATCCATTTGCCGGTACGGGAAGCTGATACCGGCGGCATCAAATTCCCGTTTAATACGc                             >  2:270915/1‑150 (MQ=255)
                                                                                                                           gAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTc                           >  2:507862/1‑150 (MQ=255)
                                                                                                                             aaaaCTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCa                         <  2:1469758/150‑1 (MQ=255)
                                                                                                                              aaaCTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAg                        >  2:119233/1‑150 (MQ=255)
                                                                                                                                aCTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGca                      <  1:616936/150‑1 (MQ=255)
                                                                                                                                   aataaGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACAt                   >  1:1677783/1‑150 (MQ=255)
                                                                                                                                    ataaGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATc                  <  2:947198/150‑1 (MQ=255)
                                                                                                                                     taaGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGCGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGATTAATACGCTCCAGCACATcc                 >  2:1568252/1‑150 (MQ=255)
                                                                                                                                      aaGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATccc                <  1:878179/150‑1 (MQ=255)
                                                                                                                                      aaGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATccc                >  2:860203/1‑150 (MQ=255)
                                                                                                                                        gagagagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAg              >  2:391976/1‑150 (MQ=255)
                                                                                                                                         agagagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAATGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCCCGTTTAATACGCTCCAGCCCATCCCAgg             >  2:1089431/1‑149 (MQ=255)
                                                                                                                                            gagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTATTTCACCATCTTAAAGTTCACATCCATTTGCGGGTAAGGGAAGCTGAAACCTGCGGCAGCAAATTCACGTTTAATACGCTACAGCACATCCCAGTaca          >  2:1240838/1‑150 (MQ=255)
                                                                                                                                             cggCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTacac         <  2:139188/149‑1 (MQ=255)
                                                                                                                                             agGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTacac         <  2:1119302/150‑1 (MQ=255)
                                                                                                                                              ggCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACg        >  2:144713/1‑150 (MQ=255)
                                                                                                                                              ggCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACg        >  2:774094/1‑150 (MQ=255)
                                                                                                                                              ggCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACg        >  1:1723217/1‑150 (MQ=255)
                                                                                                                                                ccAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACGtt      >  1:1560681/1‑150 (MQ=255)
                                                                                                                                                ccAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACCTCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACGtt      >  2:1012848/1‑150 (MQ=255)
                                                                                                                                                ccAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCGCAGTTCACGtt      >  2:666191/1‑150 (MQ=255)
                                                                                                                                                 cAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGCTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACGttt     >  2:272702/1‑150 (MQ=255)
                                                                                                                                                    agagTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACGTTTTGc  <  2:90714/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACGTTTTGC  >  NC_000913/3068773‑3069070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: