Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 18,683 C→T 6.0% intergenic (+28/‑32) nhaA → / → nhaR sodium‑proton antiporter/transcriptional activator of nhaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091318,6830CT 6.0% 423.0 / 9.7 134intergenic (+28/‑32)nhaA/nhaRsodium‑proton antiporter/transcriptional activator of nhaA
Reads supporting (aligned to +/- strand):  ref base C (74/51);  new base T (4/4);  total (79/55)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.68e-01

Only 100 of 136 total aligned reads displayed.

CCTTTGGTAGCGTAGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCCACAAACCATTACCGGAC  >  NC_000913/18534‑18832
                                                                                                                                                     |                                                                                                                                                     
ccTTTGGTAGCGTAGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAAc                                                                                                                                                       >  1:1546558/1‑150 (MQ=255)
 cTTTGGTAGCGTAGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACg                                                                                                                                                      <  1:674532/150‑1 (MQ=255)
   ttGGTAGCGTAGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTACTTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGc                                                                                                                                                    <  2:1072156/150‑1 (MQ=255)
     ggTAGCGTAGATCCAGCACTGATTAACTGGGCGACACTCTGTTTCCTGGTCGGTTCTATCACTTCGGCTGTAATTGGATAGAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATATACGGCTc                                                                                                                                                  <  1:197240/150‑1 (MQ=255)
     ggTAGCGTAGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTc                                                                                                                                                  >  1:477818/1‑150 (MQ=255)
     ggTAGCGTAGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTc                                                                                                                                                  <  1:1709363/150‑1 (MQ=255)
      gTAGCGTAGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGTTAATTGGATACAGCTGGTTACGCTTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTcc                                                                                                                                                 >  1:83446/1‑150 (MQ=255)
           gTAGATCCAGAGCTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCtttt                                                                                                                                            >  2:633259/1‑150 (MQ=255)
           gTAGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACTGTTTACCGGGGAGACATAAAAGGCTCCCtgtt                                                                                                                                            >  2:95909/1‑150 (MQ=255)
            tAGATCAAGAACTGCTTAACTGGGCGAAACTCGGTAGCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCGTTTATGGCTccccggta                                                                                                                                           <  2:148691/150‑6 (MQ=255)
             aGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCa                                                                                                                                          >  2:1297369/1‑150 (MQ=255)
               aTCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCAtt                                                                                                                                        <  1:962569/150‑1 (MQ=255)
               aTCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCAtt                                                                                                                                        >  1:619733/1‑150 (MQ=255)
                 ccAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGACGGTTCTATCTCTTCGGCGGTAAATGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGt                                                                                                                                      >  2:1395826/1‑150 (MQ=255)
                    gAACTGATTTACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTAt                                                                                                                                   >  2:1625367/1‑150 (MQ=255)
                      cgtGATTAACTGGGCGAAACTCGGTATCGTGGTGGGTTCTATCGCTTCGGCGGTAATTTGATACAGCTGGTTACGGGTTCGTTTGCGTCAATCAGTTTGTCAGGACGGTTTACCGGGTAGCCATATACGGCTCCCTTTTCATTGTTATCa                                                                                                                                 <  1:1442215/148‑1 (MQ=255)
                      aCTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCa                                                                                                                                 >  1:1394143/1‑150 (MQ=255)
                        tGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGTCGGTTTACCGGGGAGCCATAATCGGCTGACTTTTCATTGTTATCAgg                                                                                                                               >  1:71355/1‑150 (MQ=255)
                         gATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAggg                                                                                                                              >  1:352044/1‑150 (MQ=255)
                         gATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAggg                                                                                                                              >  2:867434/1‑150 (MQ=255)
                           ttAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGgag                                                                                                                            <  1:1075999/150‑1 (MQ=255)
                             aaCTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGgagag                                                                                                                          >  2:123574/1‑150 (MQ=255)
                              aCTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGgagaga                                                                                                                         >  1:563245/1‑150 (MQ=255)
                              aCTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGgagaga                                                                                                                         <  2:1069316/150‑1 (MQ=255)
                               cTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGaa                                                                                                                        >  1:543437/1‑150 (MQ=255)
                                   gCGAAACTCAGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGGTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATgg                                                                                                                    >  2:1421324/1‑149 (MQ=255)
                                    cGAAACTCGGTATCCTGGTCGGTTCTTTCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTGTACCGGGGAGCCATAAACGGCTCCCTTTTCAGTGTTATCAGGGAGAGAAATGAg                                                                                                                   >  2:123643/1‑150 (MQ=255)
                                        aCTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATg                                                                                                               <  2:1434622/150‑1 (MQ=255)
                                        aCTAGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATg                                                                                                               <  2:695523/150‑1 (MQ=255)
                                         cTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGt                                                                                                              >  1:475581/1‑150 (MQ=255)
                                          tCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCGTTTATGGCTccccgg                                                                                                                                             >  2:1299858/1‑115 (MQ=255)
                                          tCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGtc                                                                                                             >  2:1387589/1‑150 (MQ=255)
                                            ggTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGtctc                                                                                                           >  1:1252210/1‑150 (MQ=255)
                                            ggTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGtctc                                                                                                           <  2:1608791/150‑1 (MQ=255)
                                             gTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCa                                                                                                          >  1:999655/1‑150 (MQ=255)
                                               aTCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCata                                                                                                        <  2:555621/150‑1 (MQ=255)
                                                tCCTGGTCGGTTCTATCTCTTCTGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATAAGTTTGACAGGCCGGTTTACCGGGGCGCCATAAACGGCTCCCTGTTCAGTGTTATCAGGGAGAGAAATGAGCAGGTCTCatat                                                                                                       >  2:339983/1‑150 (MQ=255)
                                                tCCTGGTCGGTTCTATCGCTTCGGCGGTAATTGGCTACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATTAGCATGTCTCatat                                                                                                       <  2:700664/150‑1 (MQ=255)
                                                 ccTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATc                                                                                                      <  1:694920/150‑1 (MQ=255)
                                                   tGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCaa                                                                                                    >  1:1184236/1‑150 (MQ=255)
                                                    ggTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAAt                                                                                                   >  1:1276480/1‑150 (MQ=255)
                                                     gTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAAtt                                                                                                  >  2:453677/1‑150 (MQ=255)
                                                      gcGGTTCTATCTCTTCGGAGGTAATTGGATACAGCTGGTTCCGCGGTCGTTTGCGTCCAACAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTa                                                                                                 <  1:1059370/149‑1 (MQ=255)
                                                                ctctTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTg                                                                                       >  2:92030/1‑150 (MQ=255)
                                                                ctctTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTg                                                                                       <  2:312214/150‑1 (MQ=255)
                                                                 tctTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGt                                                                                      <  1:1244411/150‑1 (MQ=255)
                                                                    tCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTAtt                                                                                   <  2:563245/150‑1 (MQ=255)
                                                                     cggcggTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTa                                                                                  <  2:917671/150‑1 (MQ=255)
                                                                      ggcggcAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTAc                                                                                 <  2:1369658/150‑1 (MQ=255)
                                                                       gcggTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACt                                                                                <  2:222789/150‑1 (MQ=255)
                                                                        cggTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACtt                                                                               >  1:755127/1‑150 (MQ=255)
                                                                            aaTTGGATACAGCGGGTTACGCGTTCGTTTGCGTCACTGAGTTTGACAGGACGGTTTACCGGGGCGCCATAAACTGCTCCCTTATCAAAGATTTCAGGGAGATAAATGAGCATATCTAATAGCAATAACAAAAAAATGTATTACttaagc                                                                           >  2:163962/1‑146 (MQ=255)
                                                                              ttGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCa                                                                         >  1:686685/1‑150 (MQ=255)
                                                                                     cAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCtat                                                                  >  1:757093/1‑150 (MQ=255)
                                                                                             tACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAgg                                                          >  1:1126312/1‑150 (MQ=255)
                                                                                             tACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAgg                                                          <  2:379231/150‑1 (MQ=255)
                                                                                                 cgttcgttTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTcc                                                      >  1:1557662/1‑150 (MQ=255)
                                                                                                 cgttcgttTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTcc                                                      >  2:80090/1‑150 (MQ=255)
                                                                                                 cgttcgttTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTAGGGCATGTCTATAAAGAAGGTTcc                                                      >  1:1692871/1‑150 (MQ=255)
                                                                                                  gttcgttTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATATAGAAGGTTCCg                                                     >  2:1253871/1‑150 (MQ=255)
                                                                                                      gttTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGt                                                 >  1:986878/1‑150 (MQ=255)
                                                                                                        ttGCGTCCATCAGTTTGACAGGACGGTTTACGGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTg                                               <  2:83446/150‑1 (MQ=255)
                                                                                                         tGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTAATATCAATTAAAACCAATTGTATTACTTCTGTAATGTCTATAAAGAAGGTTCCGTGTTTgg                                              >  1:510833/1‑150 (MQ=255)
                                                                                                           cGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGgcg                                            >  2:411924/1‑150 (MQ=255)
                                                                                                           cGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGgcg                                            >  1:1346391/1‑150 (MQ=255)
                                                                                                           cGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCAGGTCTCATATCAATTACAACCACTTGTAGTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGgcg                                            >  2:1727023/1‑150 (MQ=255)
                                                                                                           cGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCANNNACGNNTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGgcg                                            >  2:1733619/1‑150 (MQ=255)
                                                                                                            gTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGgcgc                                           <  1:703042/150‑1 (MQ=255)
                                                                                                            gTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGgcgc                                           <  1:829755/150‑1 (MQ=255)
                                                                                                               cATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGTAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGTTTCCGTGTTTGGCGCAGc                                        >  1:1088399/1‑150 (MQ=255)
                                                                                                               cATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGc                                        >  2:648622/1‑150 (MQ=255)
                                                                                                               cATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTGTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGc                                        <  1:508878/150‑1 (MQ=255)
                                                                                                               cATCAGTTTGACAGGACGGATTACCGGGGAGCCATAAACGGCTCCCATTTCATTGTTTTCAGGGAGAGAAATGAGCATGTCACAAATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATATAGAAGGTTCCGTGGTTGGCGCAGc                                        >  2:504503/1‑150 (MQ=255)
                                                                                                                aTCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCg                                       >  2:353064/1‑150 (MQ=255)
                                                                                                                aTCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAGGTTCCGTGGTTTGCGCAGccg                                      >  2:901275/1‑148 (MQ=255)
                                                                                                                 tCAGTTTGACAGGACGGGTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCgg                                      <  2:971274/150‑1 (MQ=255)
                                                                                                                  cAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGa                                     >  2:628709/1‑150 (MQ=255)
                                                                                                                         aCAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCtt                              >  1:1148766/1‑150 (MQ=255)
                                                                                                                          cAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCttt                             <  1:709932/150‑1 (MQ=255)
                                                                                                                            ggACTGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCtttat                           <  2:392299/150‑1 (MQ=255)
                                                                                                                            ggACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCtttat                           >  1:431234/1‑150 (MQ=255)
                                                                                                                            ggACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCtttat                           >  2:1169556/1‑150 (MQ=255)
                                                                                                                            ggACGGTTTACCGGGGAGCCATAAACGGCTCCCGTTTCATTGTTATGAGGGAGAGAAATGAGCATGTCTCATTTCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCtttat                           <  2:1528608/150‑1 (MQ=255)
                                                                                                                              aCGGTTTACCGGGGAGCCATAATCGGCTCCCTGTTCATTGTTATCAGGGAGAGAAATGAGCATTTCTCATATCAATTACAACCAATTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCtttattt                         <  2:1321476/150‑1 (MQ=255)
                                                                                                                              aCGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCtttattt                         <  2:398964/150‑1 (MQ=255)
                                                                                                                              aCGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCtttattt                         <  2:388404/150‑1 (MQ=255)
                                                                                                                                 gTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAAc                      >  2:809455/1‑150 (MQ=255)
                                                                                                                                 gTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTACTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGATGGCGCAGCGGAGGCGCTTTATTTAAc                      <  2:1003212/150‑1 (MQ=255)
                                                                                                                                      ccGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCcac                 <  1:62216/150‑1 (MQ=255)
                                                                                                                                      ccGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCcac                 <  1:1710804/150‑1 (MQ=255)
                                                                                                                                      ccGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTGGGCGCAGCGGAGGCGCTTTATTTAACTCcac                 <  1:255536/150‑1 (MQ=255)
                                                                                                                                      aaagggAGCCGTTTATGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCAGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCcac                 >  1:999225/4‑150 (MQ=255)
                                                                                                                                        ggggAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCCACaa               >  2:292124/1‑150 (MQ=255)
                                                                                                                                         gggAGCCGTTTATGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACCACCACTTGTATTACTTCTGGAATGACTATAAAGAAGGTTCCGTGGTTGGAGCAGCGGAGGCGCTTTATTTAACTCCACaaa              >  1:92766/1‑150 (MQ=255)
                                                                                                                                              ccATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCCACAAACCAtt         <  2:475581/150‑1 (MQ=255)
                                                                                                                                               cATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTAATTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCCACAAACCATTa        >  1:58744/1‑150 (MQ=255)
                                                                                                                                                aTAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTGATTTAACTCCACAAACCATTAc       >  2:832660/1‑150 (MQ=255)
                                                                                                                                                 tAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCCACAAACCATTAcc      <  2:803045/150‑1 (MQ=255)
                                                                                                                                                  aaaCGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCCACAAACCATTACCg     <  1:61807/150‑1 (MQ=255)
                                                                                                                                                     cGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCCACAAACCATTACCGGAc  >  1:877/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCTTTGGTAGCGTAGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATGTCTCATATCAATTACAACCACTTGTATTACTTCTGGCATGTCTATAAAGAAGGTTCCGTGGTTGGCGCAGCGGAGGCGCTTTATTTAACTCCACAAACCATTACCGGAC  >  NC_000913/18534‑18832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: