Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,073,001 C→T 5.9% E231K (GAA→AAA)  epd ← D‑erythrose 4‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,073,0010CT 5.9% 435.7 / 9.8 137E231K (GAA→AAA) epdD‑erythrose 4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (66/62);  new base T (2/6);  total (69/68)
Fisher's exact test for biased strand distribution p-value = 2.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.46e-01

Only 100 of 142 total aligned reads displayed.

TTCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCATCAATAACCTGTTGATCGTGCATGGCGGAGT  >  NC_000913/3072852‑3073147
                                                                                                                                                     |                                                                                                                                                      
ttCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTc                                                                                                                                                        <  2:1034982/150‑1 (MQ=255)
ttCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTc                                                                                                                                                        <  1:748834/150‑1 (MQ=255)
atCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTc                                                                                                                                                        <  1:1167302/149‑1 (MQ=255)
  ccGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCaa                                                                                                                                                      >  1:1046761/1‑150 (MQ=255)
   cGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCaaa                                                                                                                                                     >  2:746878/1‑150 (MQ=255)
    gTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTATTGGTGGCCCACGTACCGCCATAGCTTGAAAGc                                                                                                                                                   >  1:652742/1‑150 (MQ=255)
         gTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATc                                                                                                                                               <  2:1419346/150‑1 (MQ=255)
         gTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATc                                                                                                                                               >  2:595996/1‑150 (MQ=255)
          tCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCg                                                                                                                                              <  1:237106/150‑1 (MQ=255)
           cAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTTGAAGCGAttgc                                                                                                                                             <  2:1368816/150‑4 (MQ=255)
           cAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGt                                                                                                                                             <  1:1003306/150‑1 (MQ=255)
           cAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAAAAGGGTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGt                                                                                                                                             >  1:380260/1‑150 (MQ=255)
            aaCTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGtt                                                                                                                                            >  2:506904/1‑150 (MQ=255)
              cTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTTGAAGCGAttgcggt                                                                                                                                          <  1:612174/150‑7 (MQ=255)
              cTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTTGAAGCGAttgcggt                                                                                                                                          <  1:396604/150‑7 (MQ=255)
               tataCCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTaaa                                                                                                                                         <  2:556171/150‑1 (MQ=255)
                   ccATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGc                                                                                                                                     >  1:1032557/1‑150 (MQ=255)
                   ccATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGc                                                                                                                                     <  2:164720/150‑1 (MQ=255)
                   ccATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTAACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGc                                                                                                                                     >  1:446492/1‑150 (MQ=255)
                    cATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCg                                                                                                                                    <  1:1460085/150‑1 (MQ=255)
                     aTGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCgg                                                                                                                                   >  1:1466214/1‑150 (MQ=255)
                          aTGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGaaaaa                                                                                                                              <  2:1495395/150‑1 (MQ=255)
                              aCCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCATCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCg                                                                                                                          >  1:50244/1‑150 (MQ=255)
                               ccTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCgt                                                                                                                         >  2:1111764/1‑150 (MQ=255)
                               ccTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCgt                                                                                                                         >  2:438570/1‑150 (MQ=255)
                                cTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCgtg                                                                                                                        <  2:1632308/150‑1 (MQ=255)
                                  tgtgCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGtgtg                                                                                                                      <  2:95792/150‑1 (MQ=255)
                                  tgtgCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGATGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGtgtg                                                                                                                      >  2:471921/1‑150 (MQ=255)
                                   ctgctgcTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCAACGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGa                                                                                                                     >  1:1065297/2‑150 (MQ=255)
                                      ctgctTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATAc                                                                                                                  >  2:441419/1‑150 (MQ=255)
                                       tgctTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATAcc                                                                                                                 <  1:1066149/150‑1 (MQ=255)
                                        gctTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACcg                                                                                                                >  2:1132329/1‑150 (MQ=255)
                                           ttttGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACcggcg                                                                                                             <  2:170353/150‑1 (MQ=255)
                                             ttGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGGCACGCTTAAATCGATTGCCGTCACATTTATTGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCggcggc                                                                                                           <  1:280679/150‑1 (MQ=255)
                                              tGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGcc                                                                                                          >  2:1509882/1‑150 (MQ=255)
                                              tGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGcc                                                                                                          >  2:1418187/1‑150 (MQ=255)
                                                cagcaACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAg                                                                                                        <  2:694765/150‑1 (MQ=255)
                                                cagcaACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAg                                                                                                        <  1:1671405/150‑1 (MQ=255)
                                                cagcaACAGGTTGACTTCATTGGCTGTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTTCCGTCACATTTATGTTTGGCACACGTACCGCAATCGCTTCACAGCGAGCGTGAAATTGCGGCAAAAATCGTCTGAAACCGGCTGcccg                                                                                                        >  2:306418/1‑148 (MQ=255)
                                                  gcaACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAAATGCGGAAAAAATCGTGTGATACCGGCGGCCAGtt                                                                                                      >  1:1078702/1‑150 (MQ=255)
                                                   caACAGGTTGACATCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGttt                                                                                                     <  2:1241899/150‑1 (MQ=255)
                                                       aGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATAcc                                                                                                                 <  2:1222618/134‑1 (MQ=255)
                                                       aGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATAcc                                                                                                                 >  1:1222618/1‑134 (MQ=255)
                                                        ggTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTAt                                                                                                >  1:735044/1‑150 (MQ=255)
                                                               ttCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGTGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCgg                                                                                         >  1:1034819/1‑150 (MQ=255)
                                                               atCATTGGCTTTTACAGGGTTCTTCAGCGTCACGGTTAAATCGATTGCCGTGACATTTATGGTTGGCACTCGTACCGAAATCGCTTCAAAGCGATCGTTAAATTTCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCgg                                                                                         <  2:304940/149‑1 (MQ=255)
                                                                 cATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGaa                                                                                       <  1:942948/150‑1 (MQ=255)
                                                                      gCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATc                                                                                  <  2:691711/150‑1 (MQ=255)
                                                                       cTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCg                                                                                 >  1:1005040/1‑150 (MQ=255)
                                                                         tttACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑c                                                                           <  1:438570/150‑1 (MQ=255)
                                                                           tACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACAGGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑ctg                                                                         <  2:1198466/150‑1 (MQ=255)
                                                                            aCAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑ctgg                                                                        >  1:840568/1‑150 (MQ=255)
                                                                            aCAGGTCTCTTCACCGTCACGCTTAACTCGATTCCAGGCACCTTTATGGCTGGCACACGTACCGCAATCGCTTCAACGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGATTAGTATCGACCGGAATGATCGC‑‑‑‑ctgg                                                                        <  2:1148135/150‑1 (MQ=255)
                                                                               ggTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑ctggctg                                                                     >  2:877956/1‑150 (MQ=255)
                                                                               ggTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑ctggctg                                                                     >  1:1106148/1‑150 (MQ=255)
                                                                               ggTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑ctggctg                                                                     <  2:470153/150‑1 (MQ=255)
                                                                                gTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑ctggctgg                                                                    >  1:963171/1‑150 (MQ=255)
                                                                                    cttcACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGc                                                                >  2:316635/1‑150 (MQ=255)
                                                                                    cttcACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGc                                                                >  2:1005942/1‑150 (MQ=255)
                                                                                    cttcACCGTCACGCTTAAATCGATTGACGTCACATTTATGGTTGGCACACGTACAGAAAACTCTACAAAGCCATCGTTAACTTGCGGAAACAATCGTTTTCTACCGGCTGAAATTTAATTATCTACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGc                                                                >  2:258290/1‑150 (MQ=255)
                                                                                    cttcACCGTCACGCTTAAATCGATTACCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGc                                                                <  2:554120/150‑1 (MQ=255)
                                                                                     ttcACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGcc                                                               <  2:938601/150‑1 (MQ=255)
                                                                                      tcACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGccc                                                              >  2:592424/1‑150 (MQ=255)
                                                                                        aCCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCgg                                                            >  2:60018/1‑150 (MQ=255)
                                                                                           gTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTg                                                         <  2:243347/150‑1 (MQ=255)
                                                                                            gcACGCTTAAATCGATAGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGGGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGc                                                        <  1:249836/149‑1 (MQ=255)
                                                                                                 cTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGc                                                   >  2:1666742/1‑150 (MQ=255)
                                                                                                 cTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGAACGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGc                                                   >  1:175560/1‑150 (MQ=255)
                                                                                                    aaaTCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAgg                                                <  2:138241/150‑1 (MQ=255)
                                                                                                    aaaTCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAgg                                                <  2:530172/150‑1 (MQ=255)
                                                                                                     aaTCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGt                                               <  1:1434379/150‑1 (MQ=255)
                                                                                                      aTCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTc                                              >  2:1500634/1‑150 (MQ=255)
                                                                                                        cGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAg                                            <  1:483025/150‑1 (MQ=255)
                                                                                                          aTTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGa                                          >  1:1425457/1‑150 (MQ=255)
                                                                                                          aTTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTAAGTATCGACCGGAATGATCTA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGa                                          <  1:198643/150‑1 (MQ=255)
                                                                                                              ccGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGt                                      <  1:1424179/150‑1 (MQ=255)
                                                                                                                gTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTAt                                    <  1:930002/150‑1 (MQ=255)
                                                                                                                    caTTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCAt                                >  1:313411/1‑150 (MQ=255)
                                                                                                                     aTTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCATc                               >  1:1021126/1‑150 (MQ=255)
                                                                                                                     aTTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCATc                               <  2:1104796/150‑1 (MQ=255)
                                                                                                                      tttATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGTGACGCAGGTCAGGATGGTATGCATCa                              <  2:1065297/150‑1 (MQ=255)
                                                                                                                        tATGGTTGGCACACGTACCGCAATCGGTTCAAAGCGATCGTTAAATTGCGGTAAAAATCGTGTGATACCGGCGTCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCATCAAt                            <  1:220946/150‑1 (MQ=255)
                                                                                                                        tATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCATCAAt                            >  1:651866/1‑150 (MQ=255)
                                                                                                                             ttGGCACACGTACCGCAATCGCTTCAAAGCGTTCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCATCAATAACCt                       <  2:901317/150‑1 (MQ=255)
                                                                                                                                 cacaCGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCATCAATAACCTGTTg                   <  2:495935/150‑1 (MQ=255)
                                                                                                                                  acacGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCATCAATAACCTGTTGa                  >  1:557518/1‑150 (MQ=255)
                                                                                                                                     cGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCATCAATAACCTGTTGATCg               >  1:488705/1‑150 (MQ=255)
                                                                                                                                       tACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCATCAATAACCTGTTGATCGTg             <  1:1636758/150‑1 (MQ=255)
                                                                                                                                       tACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCATCAATAACCTGTTGATCGTg             >  1:1661651/1‑150 (MQ=255)
                                                                                                                                       tACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCATCAATAACCTGTTGATCGTg             <  1:813322/150‑1 (MQ=255)
                                                                                                                                       tACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCATCAATAACCTGTTGATCGTg             <  2:925241/150‑1 (MQ=255)
                                                                                                                                        aCCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCATCAATAACCTGTTGATCGTGc            >  2:923499/1‑150 (MQ=255)
                                                                                                                                         ccGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCATCAATAACCTGTTGATCGTGCa           >  2:1531460/1‑150 (MQ=255)
                                                                                                                                          aacgaTCGCTTTGAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCATCAATAACCTGTTGATCGTGCAt          >  2:897928/5‑150 (MQ=255)
                                                                                                                                            cAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACTGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCATCAATAACCTGTTTATAGTGCATgg        >  2:708520/1‑150 (MQ=255)
                                                                                                                                                 gCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCATCAATAACCTGTTGATCGTGCATGGCGGAg   <  2:200666/150‑1 (MQ=255)
                                                                                                                                                 gCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCATCAATAACCTGTTGATCGTGCATGGCGGAg   >  2:1658625/1‑150 (MQ=255)
                                                                                                                                                  cTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCATCAATAACCTGTTGATCGTGCATGGCGGAGt  <  2:1106148/150‑1 (MQ=255)
                                                                                                                                                  cTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGGTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCCTCAATACCCTGTGGATCGTGCATGGCGGAGt  >  1:475917/1‑150 (MQ=255)
                                                                                                                                                     cAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGCCCGGAATGATCGACTGGCTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCATCAATAACCTGTTGATCGTGCATGGCGGAg   >  2:1006240/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TTCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTCACGCTTAAATCGATTGCCGTCACATTTATGGTTGGCACACGTACCGCAATCGCTTCAAAGCGATCGTTAAATTGCGGAAAAAATCGTGTGATACCGGCGGCCAGTTTAGTATCGACCGGAATGATCGA‑‑‑‑CTGGCTGGCTGCCCGGGTGCGACGCAGGTCAGGATGGTATGCATCAATAACCTGTTGATCGTGCATGGCGGAGT  >  NC_000913/3072852‑3073147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: