Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,082,709 C→A 5.1% intergenic (+38/+168) loiP → / ← speB Phe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; Era‑binding heat shock protein/agmatinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,082,7090CA 5.1% 304.9 / 4.9 97intergenic (+38/+168)loiP/speBPhe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; Era‑binding heat shock protein/agmatinase
Reads supporting (aligned to +/- strand):  ref base C (45/47);  new base A (3/2);  total (48/49)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.69e-01

ACTGGCAAAACTGGAAGAAGGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGT  >  NC_000913/3082560‑3082858
                                                                                                                                                     |                                                                                                                                                     
aCTGGCAAAACTGGAAGAAGGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTc                                                                                                                                                       <  1:248577/150‑1 (MQ=255)
   cgCAAAACTGGAAGAAGGTCGCCAAAGCTCAAAGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCAcg                                                                                                                                                    >  1:832725/2‑150 (MQ=255)
    gCAAAACTGGAAGAACGTCGCCAAAGCTCATTGTATGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGGCGAACGTAGGTCAGATAAGGCGTTCAcgc                                                                                                                                                   <  2:1059569/150‑1 (MQ=255)
     cAAAACTGGAAGAAGGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGATCGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCAcgcc                                                                                                                                                  <  2:1625690/150‑1 (MQ=255)
     cAAAACTGGAAGAAGGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAAAGTAGGTCAGATAAGGCGTTCAcgcc                                                                                                                                                  >  1:1565870/1‑150 (MQ=255)
         aCTGGAAGAAGGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCAt                                                                                                                                              >  1:612814/1‑150 (MQ=255)
             gaagaaGGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGa                                                                                                                                          >  2:325849/1‑150 (MQ=255)
              aagaagGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATGCGGCGTGAACGCCTTATCTGAc                                                                                                                                         <  2:854896/150‑1 (MQ=255)
              aagaagGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGAc                                                                                                                                         <  1:523143/150‑1 (MQ=255)
               agaagGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACa                                                                                                                                        <  2:689374/150‑1 (MQ=255)
                  agGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATGCGGCGTGAACGCCTTATCTGAc                                                                                                                                         >  2:1547296/1‑146 (MQ=255)
                  agGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATGCGGCGTGAACGCCTTATCTGAc                                                                                                                                         <  1:1547296/146‑1 (MQ=255)
                   ggTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGCACGTAGGTCAGATAAGGCGTTCCCGCCGCAGCTGACATGaa                                                                                                                                    >  2:1166740/1‑150 (MQ=255)
                      cGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATgg                                                                                                                                 >  1:1510520/1‑150 (MQ=255)
                        ccAAAGCTCAATGTTTGACGACAATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCATGGGGGTGGCGAACGTAGGTCAGATAAGGCGTACACGCCGCATCTGACATGAATGGca                                                                                                                               >  1:108462/1‑150 (MQ=255)
                         cAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGcac                                                                                                                              >  2:1281724/1‑150 (MQ=255)
                           aaGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCACAAAACGCGTTCACGCCGCATCTGACATGAATGCCACAt                                                                                                                            <  2:950955/150‑1 (MQ=255)
                              cTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTg                                                                                                                         >  2:1369770/1‑150 (MQ=255)
                                caagttttGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGGCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGtt                                                                                                                       <  1:840810/145‑1 (MQ=255)
                                cAATGTTTGTCGTCCTTCGTGCATCCGCCGAACGCGCCGAGGATATTCGCGATGGCATGAGAGCGGATGGTATTAAGTATAGCCTGGTTGTGTCGAACGTAGGTCAGATAAGGTGTTCACGGCGCTTCTGACATGAATGGCACATTTGtt                                                                                                                       <  2:936076/150‑1 (MQ=255)
                                cAATGGTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGtt                                                                                                                       <  1:835135/150‑1 (MQ=255)
                                  aTGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTAc                                                                                                                     >  1:1519793/1‑150 (MQ=255)
                                   tGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTAcc                                                                                                                    <  1:927870/150‑1 (MQ=255)
                                    gTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCt                                                                                                                   >  2:1212268/1‑150 (MQ=255)
                                           gacCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTgcgc                                                                                                            >  2:26966/1‑150 (MQ=255)
                                             ccATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCAt                                                                                                          >  2:382144/1‑150 (MQ=255)
                                             ccATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACAGTTGTTACCTTGTGCGCAt                                                                                                          >  1:114609/1‑150 (MQ=255)
                                                tCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCTCATTGc                                                                                                       <  2:513168/150‑1 (MQ=255)
                                                  cTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCg                                                                                                     >  2:977860/1‑150 (MQ=255)
                                                  cTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCg                                                                                                     >  2:458500/1‑150 (MQ=255)
                                                   tGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCgg                                                                                                    <  1:238556/150‑1 (MQ=255)
                                                      aTCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGgatg                                                                                                 >  2:198779/1‑150 (MQ=255)
                                                      aTCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGgatg                                                                                                 >  2:870639/1‑150 (MQ=255)
                                                       tCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGgatgc                                                                                                <  1:684038/150‑1 (MQ=255)
                                                       tCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGgatgc                                                                                                <  2:832725/150‑1 (MQ=255)
                                                        ccgccgAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGgatgcg                                                                                               <  2:146313/150‑1 (MQ=255)
                                                        ccgccgAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGgatgcg                                                                                               >  2:184467/1‑150 (MQ=255)
                                                             gAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCt                                                                                          <  1:1186368/150‑1 (MQ=255)
                                                                 gcgcCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGgcg                                                                                      <  2:865616/150‑1 (MQ=255)
                                                                 gcgcCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGgcg                                                                                      <  2:1621217/150‑1 (MQ=255)
                                                                 gcgcCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGAGGTGGCGAACGTAGGTCAGATAAGGCGGTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGgcg                                                                                      <  1:343564/150‑1 (MQ=255)
                                                                 gagcCGAGCATATTCGCGATCGCATGAGCGCGGATGGGTTTAAGTAAAGCCTGGTGGTGTCGAACGTAGTTCAGATAAGGCGTTCTCGGCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGgcg                                                                                      <  1:1496387/148‑1 (MQ=255)
                                                                    cccAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGgatg                                                                                                 <  2:684740/136‑1 (MQ=255)
                                                                    cccAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGgatg                                                                                                 >  1:684740/1‑136 (MQ=255)
                                                                    cccAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTg                                                                                         <  2:1705014/144‑1 (MQ=255)
                                                                    cccAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTg                                                                                         >  1:1705014/1‑144 (MQ=255)
                                                                      cAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCt                                                                                 <  2:238289/150‑1 (MQ=255)
                                                                         cATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTAt                                                                              <  1:71665/150‑1 (MQ=255)
                                                                          atatTCGCGATCGCATGAGCGCGGATGGGATTAAGTAATGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTTTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATc                                                                             <  1:248340/150‑1 (MQ=255)
                                                                           tatTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATcc                                                                            >  1:803173/1‑150 (MQ=255)
                                                                            atTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCg                                                                           >  1:1371143/1‑150 (MQ=255)
                                                                            atTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCg                                                                           >  1:584258/1‑150 (MQ=255)
                                                                              tCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGc                                                                         >  2:228579/1‑150 (MQ=255)
                                                                               cgcgATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTTGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGcc                                                                        <  2:776027/150‑1 (MQ=255)
                                                                               cgcgATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGcc                                                                        >  2:987996/1‑150 (MQ=255)
                                                                                 cgAACGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTTGTGTCGAACGTAGGTCAGTTAAGGAGTTCACGCCGCATATGACATGAATGGCACATTTGTAACCTTGTGCGCATTGCCGTATGCGATGATGGCGCATCTTATCCGGCCTa                                                                      <  2:269892/150‑1 (MQ=255)
                                                                                   aTCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACg                                                                    <  2:372347/150‑1 (MQ=255)
                                                                                       cATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTg                                                                >  2:1641896/1‑150 (MQ=255)
                                                                                          gAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCg                                                             <  2:1710290/150‑1 (MQ=255)
                                                                                            gcgcGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGaa                                                           <  2:1510520/150‑1 (MQ=255)
                                                                                             cgcgGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACACCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAAc                                                          <  2:1373864/150‑1 (MQ=255)
                                                                                               cgGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGt                                                        >  1:472091/1‑150 (MQ=255)
                                                                                                 gATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAg                                                      <  1:1378770/150‑1 (MQ=255)
                                                                                                  ttGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAgg                                                     <  1:1526525/149‑1 (MQ=255)
                                                                                                      gATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCgg                                                 >  1:1108405/1‑150 (MQ=255)
                                                                                                      gATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCgg                                                 <  2:1511819/150‑1 (MQ=255)
                                                                                                       aTTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGa                                                >  1:656111/1‑150 (MQ=255)
                                                                                                         tAAGTAAAGCCTGGTGGTGTCGAACGTAGGGCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATa                                              <  1:1524547/150‑1 (MQ=255)
                                                                                                           aGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAgg                                                     <  2:1271827/141‑1 (MQ=255)
                                                                                                           aGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAgg                                                     >  1:1271827/1‑141 (MQ=255)
                                                                                                             tAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGc                                          <  2:612814/150‑1 (MQ=255)
                                                                                                                    tggtggtggCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCAcg                                   <  2:1257840/150‑1 (MQ=255)
                                                                                                                    tggtggTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCAcg                                   >  2:1503472/1‑150 (MQ=255)
                                                                                                                        ggtgTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCAcgccgc                               >  2:770870/1‑150 (MQ=255)
                                                                                                                         gtgtCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCa                              >  1:736605/1‑150 (MQ=255)
                                                                                                                           gtCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATc                            >  2:1038393/1‑150 (MQ=255)
                                                                                                                           gtCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATc                            >  2:1571101/1‑150 (MQ=255)
                                                                                                                           gtCGAACGTAGGTCAGATAAGGCGTTCACGCCGCAACTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATc                            <  2:1452906/150‑1 (MQ=255)
                                                                                                                            gcGAACGGACGTCAGATATGGCGTTCAGGCCGCATGTGACATGAATGGCACATTGGTTACCTTGTGCGCATTGGCGGATGCGATGGTGGCGCATCTTATCCGGGCTACGGGTGCCGATCGTAGGTCGGATAAGGCGTTCACGCCGCATcc                           <  2:875833/149‑1 (MQ=255)
                                                                                                                             cGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCg                          >  2:788633/1‑150 (MQ=255)
                                                                                                                               aacggcggTCAGATAAGGCGTTCACGCCGCATCTGACAGGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATGCGGCGTGAACGCCTTATCCGAc                        <  2:1523694/144‑1 (MQ=38)
                                                                                                                               aaCGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGAc                        <  2:1044323/150‑1 (MQ=255)
                                                                                                                               aaCGTAGGTCAGATAAGGAGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGAc                        <  2:1687076/150‑1 (MQ=255)
                                                                                                                                 ttcatgTCAGATGCGGCGTGAACGCCTTATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAt                      >  2:811241/6‑150 (MQ=38)
                                                                                                                                  gTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATGCGGCGTGAACGCCTTATCCGAccta                     <  1:429849/150‑4 (MQ=38)
                                                                                                                                    atgTCAGATGCGGCGTGAACGCCTTATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTaa                   >  2:1473917/3‑150 (MQ=38)
                                                                                                                                    aGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTaa                   >  2:649712/1‑150 (MQ=255)
                                                                                                                                    aGGTCAGACAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTaa                   <  1:501950/150‑1 (MQ=255)
                                                                                                                                     ggTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAAt                  >  2:474544/1‑150 (MQ=255)
                                                                                                                                       tCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATgg                <  1:228579/150‑1 (MQ=255)
                                                                                                                                        cAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGc               >  2:1283296/1‑150 (MQ=255)
                                                                                                                                          gATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCAc             >  1:949074/1‑150 (MQ=255)
                                                                                                                                          gATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCAc             >  1:1235410/1‑150 (MQ=255)
                                                                                                                                           aTAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACg            <  1:987996/150‑1 (MQ=255)
                                                                                                                                            tAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGt           >  2:426582/1‑150 (MQ=255)
                                                                                                                                             aaGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGtt          >  1:498109/1‑150 (MQ=255)
                                                                                                                                              aGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGttt         >  1:1277516/1‑150 (MQ=255)
                                                                                                                                                 cGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTAc      <  1:455544/150‑1 (MQ=255)
                                                                                                                                                     cACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGt  >  2:1080193/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACTGGCAAAACTGGAAGAAGGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGT  >  NC_000913/3082560‑3082858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: