Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,082,716 C→T 5.2% intergenic (+45/+161) loiP → / ← speB Phe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; Era‑binding heat shock protein/agmatinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,082,7160CT 5.2% 304.5 / 3.5 95intergenic (+45/+161)loiP/speBPhe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; Era‑binding heat shock protein/agmatinase
Reads supporting (aligned to +/- strand):  ref base C (45/45);  new base T (3/2);  total (48/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.39e-01

ACTGGAAGAAGGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCAT  >  NC_000913/3082569‑3082864
                                                                                                                                                   |                                                                                                                                                    
aCTGGAAGAAGGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCAt                                                                                                                                                    >  1:612814/1‑150 (MQ=255)
    gaagaaGGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGa                                                                                                                                                >  2:325849/1‑150 (MQ=255)
     aagaagGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATGCGGCGTGAACGCCTTATCTGAc                                                                                                                                               <  2:854896/150‑1 (MQ=255)
     aagaagGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGAc                                                                                                                                               <  1:523143/150‑1 (MQ=255)
      agaagGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACa                                                                                                                                              <  2:689374/150‑1 (MQ=255)
         agGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATGCGGCGTGAACGCCTTATCTGAc                                                                                                                                               >  2:1547296/1‑146 (MQ=255)
         agGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATGCGGCGTGAACGCCTTATCTGAc                                                                                                                                               <  1:1547296/146‑1 (MQ=255)
          ggTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGCACGTAGGTCAGATAAGGCGTTCCCGCCGCAGCTGACATGaa                                                                                                                                          >  2:1166740/1‑150 (MQ=255)
             cGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATgg                                                                                                                                       >  1:1510520/1‑150 (MQ=255)
               ccAAAGCTCAATGTTTGACGACAATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCATGGGGGTGGCGAACGTAGGTCAGATAAGGCGTACACGCCGCATCTGACATGAATGGca                                                                                                                                     >  1:108462/1‑150 (MQ=255)
                cAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGcac                                                                                                                                    >  2:1281724/1‑150 (MQ=255)
                  aaGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCACAAAACGCGTTCACGCCGCATCTGACATGAATGCCACAt                                                                                                                                  <  2:950955/150‑1 (MQ=255)
                     cTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTg                                                                                                                               >  2:1369770/1‑150 (MQ=255)
                       caagttttGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGGCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGtt                                                                                                                             <  1:840810/145‑1 (MQ=255)
                       cAATGTTTGTCGTCCTTCGTGCATCCGCCGAACGCGCCGAGGATATTCGCGATGGCATGAGAGCGGATGGTATTAAGTATAGCCTGGTTGTGTCGAACGTAGGTCAGATAAGGTGTTCACGGCGCTTCTGACATGAATGGCACATTTGtt                                                                                                                             <  2:936076/150‑1 (MQ=255)
                       cAATGGTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGtt                                                                                                                             <  1:835135/150‑1 (MQ=255)
                         aTGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTAc                                                                                                                           >  1:1519793/1‑150 (MQ=255)
                          tGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTAcc                                                                                                                          <  1:927870/150‑1 (MQ=255)
                           gTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCt                                                                                                                         >  2:1212268/1‑150 (MQ=255)
                                  gacCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTgcgc                                                                                                                  >  2:26966/1‑150 (MQ=255)
                                    ccATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCAt                                                                                                                >  2:382144/1‑150 (MQ=255)
                                    ccATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACAGTTGTTACCTTGTGCGCAt                                                                                                                >  1:114609/1‑150 (MQ=255)
                                       tCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCTCATTGc                                                                                                             <  2:513168/150‑1 (MQ=255)
                                         cTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCg                                                                                                           >  2:458500/1‑150 (MQ=255)
                                         cTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCg                                                                                                           >  2:977860/1‑150 (MQ=255)
                                          tGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCgg                                                                                                          <  1:238556/150‑1 (MQ=255)
                                             aTCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGgatg                                                                                                       >  2:198779/1‑150 (MQ=255)
                                             aTCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGgatg                                                                                                       >  2:870639/1‑150 (MQ=255)
                                              tCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGgatgc                                                                                                      <  1:684038/150‑1 (MQ=255)
                                              tCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGgatgc                                                                                                      <  2:832725/150‑1 (MQ=255)
                                               ccgccgAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGgatgcg                                                                                                     >  2:184467/1‑150 (MQ=255)
                                               ccgccgAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGgatgcg                                                                                                     <  2:146313/150‑1 (MQ=255)
                                                    gAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCt                                                                                                <  1:1186368/150‑1 (MQ=255)
                                                        gcgcCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGgcg                                                                                            <  2:865616/150‑1 (MQ=255)
                                                        gcgcCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGgcg                                                                                            <  2:1621217/150‑1 (MQ=255)
                                                        gcgcCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGAGGTGGCGAACGTAGGTCAGATAAGGCGGTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGgcg                                                                                            <  1:343564/150‑1 (MQ=255)
                                                        gagcCGAGCATATTCGCGATCGCATGAGCGCGGATGGGTTTAAGTAAAGCCTGGTGGTGTCGAACGTAGTTCAGATAAGGCGTTCTCGGCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGgcg                                                                                            <  1:1496387/148‑1 (MQ=255)
                                                           cccAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGgatg                                                                                                       <  2:684740/136‑1 (MQ=255)
                                                           cccAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGgatg                                                                                                       >  1:684740/1‑136 (MQ=255)
                                                           cccAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTg                                                                                               >  1:1705014/1‑144 (MQ=255)
                                                           cccAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTg                                                                                               <  2:1705014/144‑1 (MQ=255)
                                                             cAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCt                                                                                       <  2:238289/150‑1 (MQ=255)
                                                                cATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTAt                                                                                    <  1:71665/150‑1 (MQ=255)
                                                                 atatTCGCGATCGCATGAGCGCGGATGGGATTAAGTAATGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTTTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATc                                                                                   <  1:248340/150‑1 (MQ=255)
                                                                  tatTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATcc                                                                                  >  1:803173/1‑150 (MQ=255)
                                                                   atTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCg                                                                                 >  1:584258/1‑150 (MQ=255)
                                                                   atTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCg                                                                                 >  1:1371143/1‑150 (MQ=255)
                                                                     tCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGc                                                                               >  2:228579/1‑150 (MQ=255)
                                                                      cgcgATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTTGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGcc                                                                              <  2:776027/150‑1 (MQ=255)
                                                                      cgcgATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGcc                                                                              >  2:987996/1‑150 (MQ=255)
                                                                        cgAACGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTTGTGTCGAACGTAGGTCAGTTAAGGAGTTCACGCCGCATATGACATGAATGGCACATTTGTAACCTTGTGCGCATTGCCGTATGCGATGATGGCGCATCTTATCCGGCCTa                                                                            <  2:269892/150‑1 (MQ=255)
                                                                          aTCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACg                                                                          <  2:372347/150‑1 (MQ=255)
                                                                              cATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTg                                                                      >  2:1641896/1‑150 (MQ=255)
                                                                                 gAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCg                                                                   <  2:1710290/150‑1 (MQ=255)
                                                                                   gcgcGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGaa                                                                 <  2:1510520/150‑1 (MQ=255)
                                                                                    cgcgGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACACCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAAc                                                                <  2:1373864/150‑1 (MQ=255)
                                                                                      cgGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGt                                                              >  1:472091/1‑150 (MQ=255)
                                                                                        gATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAg                                                            <  1:1378770/150‑1 (MQ=255)
                                                                                         ttGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAgg                                                           <  1:1526525/149‑1 (MQ=255)
                                                                                             gATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCgg                                                       >  1:1108405/1‑150 (MQ=255)
                                                                                             gATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCgg                                                       <  2:1511819/150‑1 (MQ=255)
                                                                                              aTTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGa                                                      >  1:656111/1‑150 (MQ=255)
                                                                                                tAAGTAAAGCCTGGTGGTGTCGAACGTAGGGCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATa                                                    <  1:1524547/150‑1 (MQ=255)
                                                                                                  aGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAgg                                                           >  1:1271827/1‑141 (MQ=255)
                                                                                                  aGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAgg                                                           <  2:1271827/141‑1 (MQ=255)
                                                                                                    tAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGc                                                <  2:612814/150‑1 (MQ=255)
                                                                                                           tggtggtggCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCAcg                                         <  2:1257840/150‑1 (MQ=255)
                                                                                                           tggtggTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCAcg                                         >  2:1503472/1‑150 (MQ=255)
                                                                                                               ggtgTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCAcgccgc                                     >  2:770870/1‑150 (MQ=255)
                                                                                                                gtgtCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCa                                    >  1:736605/1‑150 (MQ=255)
                                                                                                                  gtCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATc                                  >  2:1571101/1‑150 (MQ=255)
                                                                                                                  gtCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATc                                  >  2:1038393/1‑150 (MQ=255)
                                                                                                                  gtCGAACGTAGGTCAGATAAGGCGTTCACGCCGCAACTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATc                                  <  2:1452906/150‑1 (MQ=255)
                                                                                                                   gcGAACGGACGTCAGATATGGCGTTCAGGCCGCATGTGACATGAATGGCACATTGGTTACCTTGTGCGCATTGGCGGATGCGATGGTGGCGCATCTTATCCGGGCTACGGGTGCCGATCGTAGGTCGGATAAGGCGTTCACGCCGCATcc                                 <  2:875833/149‑1 (MQ=255)
                                                                                                                    cGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCg                                >  2:788633/1‑150 (MQ=255)
                                                                                                                      aacggcggTCAGATAAGGCGTTCACGCCGCATCTGACAGGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATGCGGCGTGAACGCCTTATCCGAc                              <  2:1523694/144‑1 (MQ=38)
                                                                                                                      aaCGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGAc                              <  2:1044323/150‑1 (MQ=255)
                                                                                                                      aaCGTAGGTCAGATAAGGAGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGAc                              <  2:1687076/150‑1 (MQ=255)
                                                                                                                        ttcatgTCAGATGCGGCGTGAACGCCTTATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAt                            >  2:811241/6‑150 (MQ=38)
                                                                                                                         gTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATGCGGCGTGAACGCCTTATCCGAccta                           <  1:429849/150‑4 (MQ=38)
                                                                                                                           atgTCAGATGCGGCGTGAACGCCTTATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTaa                         >  2:1473917/3‑150 (MQ=38)
                                                                                                                           aGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTaa                         >  2:649712/1‑150 (MQ=255)
                                                                                                                           aGGTCAGACAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTaa                         <  1:501950/150‑1 (MQ=255)
                                                                                                                            ggTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAAt                        >  2:474544/1‑150 (MQ=255)
                                                                                                                              tCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATgg                      <  1:228579/150‑1 (MQ=255)
                                                                                                                               cAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGc                     >  2:1283296/1‑150 (MQ=255)
                                                                                                                                 gATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCAc                   >  1:949074/1‑150 (MQ=255)
                                                                                                                                 gATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCAc                   >  1:1235410/1‑150 (MQ=255)
                                                                                                                                  aTAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACg                  <  1:987996/150‑1 (MQ=255)
                                                                                                                                   tAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGt                 >  2:426582/1‑150 (MQ=255)
                                                                                                                                    aaGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGtt                >  1:498109/1‑150 (MQ=255)
                                                                                                                                     aGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGttt               >  1:1277516/1‑150 (MQ=255)
                                                                                                                                        cGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTAc            <  1:455544/150‑1 (MQ=255)
                                                                                                                                            cACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGt        >  2:1080193/1‑150 (MQ=255)
                                                                                                                                             aCGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTg       >  2:1413609/1‑150 (MQ=255)
                                                                                                                                               gccgcATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTgcg     >  2:1487644/1‑150 (MQ=255)
                                                                                                                                                  gcATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGTGTTACTAACGTATGTCGGATAAGGCGTTCACGCCCCATCCGACATTAATGGCACGATTTACCCGTGagcat  >  1:938355/1‑150 (MQ=38)
                                                                                                                                                  gcATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGcgcat  <  1:346972/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ACTGGAAGAAGGTCGCCAAAGCTCAATGTTTGACGACCATCCTGCATCCGCCGAACGCGCCCAGCATATTCGCGATCGCATGAGCGCGGATGGGATTAAGTAAAGCCTGGTGGTGTCGAACGTAGGTCAGATAAGGCGTTCACGCCGCATCTGACATGAATGGCACATTTGTTACCTTGTGCGCATTGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGGTGCCGAACGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTAATGGCACGTTTTACCCGTGCGCAT  >  NC_000913/3082569‑3082864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑