Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,124,312 G→A 5.8% T383M (ACG→ATG)  glcF ← glycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,124,3120GA 5.8% 324.4 / 5.5 102T383M (ACG→ATG) glcFglycolate oxidase 4Fe‑4S iron‑sulfur cluster subunit
Reads supporting (aligned to +/- strand):  ref base G (49/47);  new base A (4/2);  total (53/49)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 104 total aligned reads displayed.

CGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCACAGATGGCTGTCGGGAA  >  NC_000913/3124163‑3124459
                                                                                                                                                     |                                                                                                                                                   
cgcCTCTTCCTGACCTGCGGCTATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCg                                                                                                                                                     >  1:1293652/1‑150 (MQ=255)
cgcCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCg                                                                                                                                                     <  2:737675/150‑1 (MQ=255)
  ccTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTGCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTc                                                                                                                                                   >  2:1376384/1‑150 (MQ=255)
   ctctTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCt                                                                                                                                                  <  1:664491/150‑1 (MQ=255)
     ctTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTgg                                                                                                                                                <  1:1116306/150‑1 (MQ=255)
      ttCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGc                                                                                                                                               <  2:212025/150‑1 (MQ=255)
      ttCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGc                                                                                                                                               <  1:1063335/150‑1 (MQ=255)
      ttCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGc                                                                                                                                               <  1:1680840/150‑1 (MQ=255)
      ttCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGc                                                                                                                                               <  2:866628/150‑1 (MQ=255)
         cTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCTCAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAAc                                                                                                                                            >  2:159729/1‑150 (MQ=255)
         cTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGACGCATCTGGcgag                                                                                                                                            <  1:515151/150‑4 (MQ=255)
          tGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGACGCATCTGGcgagc                                                                                                                                           <  2:620511/150‑5 (MQ=255)
            aCCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCaa                                                                                                                                         >  1:1324074/1‑150 (MQ=255)
             ccTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCCTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAAt                                                                                                                                        >  2:944763/1‑150 (MQ=255)
              cTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATg                                                                                                                                       <  2:1700695/150‑1 (MQ=255)
                gCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCGCGCCAGATGCGTCTGGCAACCAATGtt                                                                                                                                     <  1:1232253/150‑1 (MQ=255)
                 cGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTg                                                                                                                                    >  1:278952/1‑150 (MQ=255)
                     aataatTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCgg                                                                                                                                <  1:423204/150‑1 (MQ=255)
                        aatTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGa                                                                                                                             >  2:1225965/1‑150 (MQ=255)
                         atTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGAc                                                                                                                            <  1:447079/150‑1 (MQ=255)
                            tcACTCGCCATTTGCTGGCGATGAAGGACTTTAGGTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCACTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGAt                                                                                                                         <  2:289242/149‑1 (MQ=255)
                            gCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACgtt                                                                                                                         >  2:23368/1‑148 (MQ=255)
                            gCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACgtt                                                                                                                         >  2:818060/1‑148 (MQ=255)
                              aCTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACACACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCa                                                                                                                       <  1:352505/150‑1 (MQ=255)
                               cTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCAt                                                                                                                      >  1:770520/1‑150 (MQ=255)
                                 ggCCTTTTTCTGTCTAAGATTGACTTTTGTTTTCATTTTGTTATTCATATTCAAGGGCTTGTTCTACAATATGAATCCAGTGACGCACAGAGGTACGAGCGGCGCTCGCCAGATGCGTCTGGCTAGCAATGTTGGCGGTGACGATAAttt                                                                                                                    <  2:1730845/149‑1 (MQ=255)
                                 cGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCAttt                                                                                                                    <  2:1132800/150‑1 (MQ=255)
                                    cATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCg                                                                                                                 >  2:1320508/1‑150 (MQ=255)
                                     aTTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCgg                                                                                                                <  1:1546197/150‑1 (MQ=255)
                                       ttGCTGGCTAAGAATGACTTTAGTTTTTATTTTGTTATTCCTTTTCATGGGCTTGTTCTTGAATTTCAATCCAGTGACGCACAGTGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAAACAATGTTGGCGGTGACGATCATTTCCGGtt                                                                                                              <  2:1611519/150‑1 (MQ=255)
                                          cTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGc                                                                                                           <  1:583108/150‑1 (MQ=255)
                                           tGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGcc                                                                                                          <  2:177301/150‑1 (MQ=255)
                                           tGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAAATTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATGATTTCCGGTTTGcc                                                                                                          <  2:1273511/150‑1 (MQ=255)
                                            ggCTAATAATGACTGTAGTTTTCATTTTGTTATTCCTTTTCAAGGGATGGTTCTACAATTTCAATCCAGTGACGCACAGATGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCg                                                                                                         <  1:1043993/150‑1 (MQ=255)
                                            ggCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCg                                                                                                         <  1:1710229/150‑1 (MQ=255)
                                            ggCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCg                                                                                                         <  2:128776/150‑1 (MQ=255)
                                            ggCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCg                                                                                                         <  1:1223203/150‑1 (MQ=255)
                                              cTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCt                                                                                                       >  1:567314/1‑150 (MQ=255)
                                              cTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCt                                                                                                       >  1:966275/1‑150 (MQ=255)
                                                  gAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGAATCACAGAGGTACGACCGGCGCTCGCCAGATGAGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTcc                                                                                                   >  1:40742/1‑150 (MQ=255)
                                                         tttAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGcat                                                                                            >  1:1401703/1‑150 (MQ=255)
                                                             gTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGcattcat                                                                                        >  2:263997/1‑150 (MQ=255)
                                                              ttttCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGcattcatt                                                                                       >  2:977675/1‑150 (MQ=255)
                                                                  cATTTTGTTATTCCTTTTCACGGGCTTGTTCTACAATTTCAAGCGAGTGACGCACAGAGGTACGTCCGGCGCTCGCCAGAAGCGTCTGGCAACCAATGTTGGCGGTGACGATGATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGt                                                                                   <  2:1391955/150‑1 (MQ=255)
                                                                  cATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCACGCCAGATTCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGt                                                                                   <  2:238417/150‑1 (MQ=255)
                                                                   aTTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGAGGTGACGGTCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGtt                                                                                  <  1:1049425/150‑1 (MQ=255)
                                                                      ttGTTATTCCTTTTCATGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCACTGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATc                                                                               <  1:1553927/150‑1 (MQ=255)
                                                                        gTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATccc                                                                             <  1:1209957/150‑1 (MQ=255)
                                                                          tATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGc                                                                           >  2:645354/1‑150 (MQ=255)
                                                                           aTTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCa                                                                          <  2:1494962/150‑1 (MQ=255)
                                                                           aTTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGAACGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCa                                                                          >  2:699500/1‑150 (MQ=255)
                                                                           aTTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGAAGCACAGAGGTACGACCGGCGCTCGCAAGATGAGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCAGCa                                                                          >  1:85381/1‑150 (MQ=255)
                                                                              ccTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCt                                                                       >  2:1248060/1‑150 (MQ=255)
                                                                              ccTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCt                                                                       >  1:269945/1‑150 (MQ=255)
                                                                                   tCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGt                                                                  <  2:407523/150‑1 (MQ=255)
                                                                                    cAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACTAACATTTCCGGTTTGCCGCTTTCCCGCGCCTTCATTTTGTTATCCCGCAGCTGGCGTg                                                                 >  2:491603/1‑150 (MQ=255)
                                                                                    cAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTg                                                                 >  2:611944/1‑150 (MQ=255)
                                                                                      aGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGcc                                                               >  2:1383810/1‑150 (MQ=255)
                                                                                      aGGGCTTGTTCAACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGcc                                                               <  2:241798/150‑1 (MQ=255)
                                                                                          cTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTATCCAGCGCATTCATTTTGTAATCCCGCAGCTGGCGTGCCAGAt                                                           >  2:1509142/1‑150 (MQ=255)
                                                                                            tGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCg                                                         <  1:1067115/150‑1 (MQ=255)
                                                                                            tGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGACGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCTTGCTAGATct                                                         <  1:1248060/150‑2 (MQ=255)
                                                                                             gTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCgg                                                        >  1:1600602/1‑150 (MQ=255)
                                                                                                   cAATTTCAATCCAGTGACGCAAAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCGTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCg                                                  >  2:1078003/1‑150 (MQ=255)
                                                                                                    aaTTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGt                                                 <  1:611944/150‑1 (MQ=255)
                                                                                                     aTTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGAtaacaa                                                <  1:23437/150‑6 (MQ=255)
                                                                                                         cAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACg                                            <  2:1642972/150‑1 (MQ=255)
                                                                                                         cAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACg                                            >  1:1370632/1‑150 (MQ=255)
                                                                                                         cAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACg                                            >  2:176798/1‑150 (MQ=255)
                                                                                                           aTCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCa                                          >  2:594624/1‑150 (MQ=255)
                                                                                                           aTCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCa                                          <  1:1376384/150‑1 (MQ=255)
                                                                                                             ccAGGGACGCACCGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCata                                        <  1:63368/150‑1 (MQ=255)
                                                                                                              cAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCAGGCGTGCCAGATCGGGATGCGTTACCGCatat                                       >  1:860646/1‑150 (MQ=255)
                                                                                                                 tGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGtt                                    <  2:412224/150‑1 (MQ=255)
                                                                                                                   aCGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTcc                                  <  2:882492/150‑1 (MQ=255)
                                                                                                                    cGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTccc                                 <  2:966275/150‑1 (MQ=255)
                                                                                                                     gCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCg                                >  2:808623/1‑150 (MQ=255)
                                                                                                                     gCACAGAGGTACGACCGGCGCGCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCg                                <  1:1109409/150‑1 (MQ=255)
                                                                                                                          gagGTAAGACCGGCGCTCGCCAGATGCGTCTGGCATCCAATGTTGGCGGTGACGCTCCTTTCCGGTTTGCCTCGTTCCAGCGCCTTCATTTTGTTCTCCCGCAGCTGGCGAGCCAGATCGGGATCCGATAACGCATATGTTCCCGCTgca                           >  1:309690/1‑148 (MQ=255)
                                                                                                                           agGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAAc                          <  1:572257/150‑1 (MQ=255)
                                                                                                                            ggTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAAcc                         >  2:927705/1‑150 (MQ=255)
                                                                                                                             gTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCg                        >  1:110174/1‑150 (MQ=255)
                                                                                                                                ccaCCGGCGCCCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGACCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGGGCCAGATCGGGATGAGTTAACGCATATGTTCCCGCCGAACCgcag                     <  2:888304/148‑1 (MQ=255)
                                                                                                                                cGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCgcag                     >  1:1669073/1‑150 (MQ=255)
                                                                                                                                 gACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCgcagc                    >  1:1344871/1‑150 (MQ=255)
                                                                                                                                   ccGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGcac                  >  2:1281741/1‑150 (MQ=255)
                                                                                                                                   ccGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGcac                  <  2:1293652/150‑1 (MQ=255)
                                                                                                                                        ggttgCCAGACGCATCTGGCGACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCCGCGCTTTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCACAGATg             >  1:182545/5‑150 (MQ=255)
                                                                                                                                        ggttgCCAGACGCATCTGGCGACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCACAGATg             >  1:1342912/5‑150 (MQ=255)
                                                                                                                                        ggttgCCAGACGCATCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCACAGATg             >  1:1644975/5‑150 (MQ=255)
                                                                                                                                         gttgCCAGACGCATCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCACAGATgg            >  1:942936/4‑150 (MQ=255)
                                                                                                                                         cTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCACAGATgg            >  1:607147/1‑150 (MQ=255)
                                                                                                                                           cGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCACAGATGGCt          >  2:775053/1‑150 (MQ=255)
                                                                                                                                            gCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCACAGATGGCTg         >  1:844827/1‑150 (MQ=255)
                                                                                                                                             ccAGATGCGTCTGGCATCCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCACAGATGGCTGt        >  1:772711/1‑150 (MQ=255)
                                                                                                                                               aGATGCGTCTGGCAACCAATGTTGGCGGTGCCGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCACAGATGGCTGTCg      >  2:1025364/1‑150 (MQ=255)
                                                                                                                                               aGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCACAGATGGCTGTCg      >  2:1460061/1‑150 (MQ=255)
                                                                                                                                                gATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCACAGATGGCTGTCgg     >  2:621908/1‑150 (MQ=255)
                                                                                                                                                   gCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCACAGATGGCTGTCGGGaa  <  1:1320508/150‑1 (MQ=255)
                                                                                                                                                   gCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCACAGATGGCTGTCGGGaa  <  2:964574/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGCGTGCCAGATCGGGATGCGTTAACGCATATGTTCCCGCTGAACCGCAGCACAGATGGCTGTCGGGAA  >  NC_000913/3124163‑3124459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: