BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,135,357	G→A	6.3%	Q339* (CAG→TAG) ‡	pitB ←	phosphate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,135,357	0	G	A	6.3%	302.9 / 6.0	97	Q339* (CAG→TAG) ‡	pitB	phosphate transporter
Reads supporting (aligned to +/- strand): ref base G (39/51); new base A (2/4); total (42/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.91e-01

Only 100 of 103 total aligned reads displayed.

ATGATGATCCACACCGGAGCGTACTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGC  >  NC_000913/3135209‑3135499

atgatgATCCACACCGGAGCGTACTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAgctgg                                                                                                                                               >  2:607642/1‑150 (MQ=255)

 tgatgaTCCACACCGGAGCGTACTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAgctggc                                                                                                                                              <  1:958952/150‑1 (MQ=255)

 tgatgaTCCACACCGGAGCGTACTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAgctggc                                                                                                                                              >  1:127660/1‑150 (MQ=255)

   atgatCCACACCGGAGCGTACTCAATGGTGCTTAACATATCGCTGCGAATTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAgctggctg                                                                                                                                            >  1:330634/1‑150 (MQ=255)

     tatCCACACCGGAGCGTACTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTgcg                                                                                                                                          <  2:193299/149‑1 (MQ=255)

     gatCCACACCGGAGCGTCCTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTCCTGACCCCTGGCAGTTTCGCAAGCTTCGAGGAGGTCTCAGAGATGAACAGCATAATGCGGCGCAGCTGGCTccg                                                                                                                                          >  2:1175198/1‑150 (MQ=255)

     gatCCACACCGGAGCGTACTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTgcg                                                                                                                                          >  2:1175199/1‑150 (MQ=255)

      atCCACACCGGAGCGTACTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGGCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCAGCGCAGCCAGCTgcgc                                                                                                                                         <  1:1284215/150‑1 (MQ=255)

       tCCACACCGGAGCGTACTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCt                                                                                                                                        <  2:378822/150‑1 (MQ=255)

       tCCACACCGGAGCGTACTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCt                                                                                                                                        <  2:372609/150‑1 (MQ=255)

        ccACACCGGAGCGTACTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACTCCTGGCAGTTTCGATAGCTTCGCGGTGGTATCAGAGATGCAAAGCATAATGCGGCGCAGATGGCTGCGATg                                                                                                                                       >  2:1477427/1‑150 (MQ=255)

         cacaCCGGAGCGTACTCAATGGGGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCCAGCTgcgccgc                                                                                                                                      <  1:872746/150‑4 (MQ=255)

          accccGGAGCGTACTCAATGGTGCTTAACATATCGCTGCGAAGCTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCGTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCCAGCTgcgccgca                                                                                                                                     <  1:1164276/147‑5 (MQ=255)

          acacCGGAGCGTACTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGAc                                                                                                                                     <  1:176977/150‑1 (MQ=255)

           cacCGGAGCGTACTCAATGGTGCTTCACATCTCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCCAGCTgcgccgcat                                                                                                                                    <  1:1537645/150‑6 (MQ=255)

              cGGAGCGTACTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCAc                                                                                                                                 <  1:954597/150‑1 (MQ=255)

               ggAGCGTACTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACg                                                                                                                                <  1:1396657/150‑1 (MQ=255)

                gAGCGTACTCACTGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGc                                                                                                                               <  2:1520545/150‑1 (MQ=255)

                  gCGTACTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCtt                                                                                                                             <  1:932016/150‑1 (MQ=255)

                   cGTACTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAgctggc                                                                                                                                              <  2:1585767/132‑1 (MQ=255)

                   cGTACTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAgctggc                                                                                                                                              >  1:1585767/1‑132 (MQ=255)

                     tACTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCTCAGCTGGCTGCGCTGACTCACGCTTAAc                                                                                                                          >  2:920488/1‑150 (MQ=255)

                       cTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCGGACTCACGCTTAACgg                                                                                                                        <  2:249024/150‑1 (MQ=255)

                       cTCAATGGTGCTTAAAATATAGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACgg                                                                                                                        <  2:1332625/150‑1 (MQ=255)

                           aTGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCg                                                                                                                    >  1:329498/1‑150 (MQ=255)

                           aTGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCg                                                                                                                    <  2:812715/150‑1 (MQ=255)

                           aTGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGACTTGCAGTTTCGCTAGCTTCGCTGAGGTATCAGAGATGCACAGCATAATGAGGCGCAGCTCGCTGCGCTGACTCACGCTTAACGGATcc                                                                                                                    >  1:377899/1‑149 (MQ=255)

                            tGGTGCTTAACATATCGCTGCGAAGTTTTTTCATCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGATGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGt                                                                                                                   <  2:277837/150‑1 (MQ=255)

                            tGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGt                                                                                                                   <  2:1701260/150‑1 (MQ=255)

                             ggTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTa                                                                                                                  <  2:160878/150‑1 (MQ=255)

                             ggTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGGCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTa                                                                                                                  <  1:925687/150‑1 (MQ=255)

                                gCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACt                                                                                                               >  2:394917/1‑150 (MQ=255)

                                 cTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACtt                                                                                                              >  1:1036731/1‑150 (MQ=255)

                                   tAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTc                                                                                                            <  2:373466/150‑1 (MQ=255)

                                     aCATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTGTCCa                                                                                                          >  2:603995/1‑150 (MQ=255)

                                      cATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCat                                                                                                         <  1:684978/150‑1 (MQ=255)

                                        tatCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCatat                                                                                                       >  2:1581250/1‑150 (MQ=255)

                                            gCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGcc                                                                                                   <  2:1604319/150‑1 (MQ=255)

                                             cTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCt                                                                                                  >  2:1176642/1‑150 (MQ=255)

                                                cGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGgc                                                                                               <  1:425870/150‑1 (MQ=255)

                                                 gAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGgca                                                                                              >  2:428797/1‑150 (MQ=255)

                                                  aaGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGgcag                                                                                             <  2:373130/150‑1 (MQ=255)

                                                  aaGTTTTTTCAGCAGGGTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCGTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGgcag                                                                                             <  1:941527/150‑1 (MQ=255)

                                                    gTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGgcagca                                                                                           <  1:1175199/150‑1 (MQ=255)

                                                       ttttCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCg                                                                                        >  2:456779/1‑150 (MQ=255)

                                                       ttttCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCg                                                                                        <  1:373943/150‑1 (MQ=255)

                                                       ttttCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCg                                                                                        <  1:330365/150‑1 (MQ=255)

                                                        tttCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCGGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGt                                                                                       <  2:1341979/150‑1 (MQ=255)

                                                         ttCAGCAGTGTCTGGTCTTCTTTACTGACGCCTGTCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACATCATAATGCGGAGCAGCTGGCTGCGCTGACTGACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGtt                                                                                      <  1:81893/150‑1 (MQ=255)

                                                           cagcagGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGattt                                                                                    >  2:563160/1‑150 (MQ=255)

                                                            agcagGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTa                                                                                   <  2:1716346/150‑1 (MQ=255)

                                                              cagGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTAGCGGAGGTATCAGATATGCACAGCATAATGCTTCGCAGCTGGCTGCGCTGACTCACGCTTAACGGATCGTAAATTTCCATATTGCCTGTCAGAATCGTTTTAAc                                                                                 >  2:1661763/1‑150 (MQ=255)

                                                              cagGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAACTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAAc                                                                                 <  1:1600820/150‑1 (MQ=255)

                                                               agGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAAcg                                                                                >  1:1411276/1‑150 (MQ=255)

                                                               agGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAAcg                                                                                >  2:904540/1‑150 (MQ=255)

                                                                    cTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACgcgcgc                                                                           >  1:238462/1‑150 (MQ=255)

                                                                     tGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCa                                                                          >  2:371378/1‑150 (MQ=255)

                                                                     tGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCa                                                                          <  2:922176/150‑1 (MQ=255)

                                                                      ggTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCaa                                                                         >  2:1253891/1‑150 (MQ=255)

                                                                      ggTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCaa                                                                         >  2:58817/1‑150 (MQ=255)

                                                                      ggTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCaa                                                                         >  2:1705274/1‑150 (MQ=255)

                                                                       gTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAAt                                                                        <  1:437801/150‑1 (MQ=255)

                                                                         cttcttTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCATCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAg                                                                      <  1:884368/150‑1 (MQ=255)

                                                                         cttcttTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTTGCAGCATCGTTTTAACGCGCGCAATAg                                                                      <  2:1160945/150‑1 (MQ=255)

                                                                           tcttTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCa                                                                    <  1:456779/150‑1 (MQ=255)

                                                                            cttTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCAt                                                                   >  2:299501/1‑150 (MQ=255)

                                                                                 cTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAACAGCATCAAAg                                                              >  1:1501451/1‑150 (MQ=255)

                                                                                   gACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGt                                                            >  1:895923/1‑150 (MQ=255)

                                                                                   gACGCCTGCCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCACGTAACTTTCCATATTGCCTCGCAGCATCGTTTAAACGCGCGCACTAGCATCAAAGGt                                                            >  2:866456/1‑150 (MQ=255)

                                                                                    aCGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTa                                                           <  2:646709/150‑1 (MQ=255)

                                                                                      gCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTAtt                                                         >  2:44771/1‑150 (MQ=255)

                                                                                       ccTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTAttt                                                        <  2:1450984/150‑1 (MQ=255)

                                                                                         tGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAGTGCGGCGCAGCTGGCTGCGCTGACTCACGCTTATCGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGc                                                      >  1:500967/1‑150 (MQ=255)

                                                                                          ggCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGcc                                                     <  2:1632310/150‑1 (MQ=255)

                                                                                             aGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGTGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGa                                                  >  1:1233027/1‑150 (MQ=255)

                                                                                             aGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGa                                                  >  2:940347/1‑150 (MQ=255)

                                                                                              gTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGAt                                                 >  1:1166609/1‑150 (MQ=255)

                                                                                               tttCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATg                                                <  2:784014/150‑1 (MQ=255)

                                                                                               tttCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATg                                                <  1:1705274/150‑1 (MQ=255)

                                                                                                         ttCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAAc                                      <  2:1731666/150‑1 (MQ=255)

                                                                                                         ttCGCGGAGGTATCAGAGAGGCACCGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTATCGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAAc                                      <  2:1163425/150‑1 (MQ=255)

                                                                                                            gcgGAGGTAT‑AGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTg                                  >  1:1111382/1‑150 (MQ=255)

                                                                                                                 ggTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTAc                              <  1:577248/150‑1 (MQ=255)

                                                                                                                 ggTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTAc                              <  2:127660/150‑1 (MQ=255)

                                                                                                                   tATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACtt                            >  1:864898/1‑150 (MQ=255)

                                                                                                                   tATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACtt                            <  1:1239669/150‑1 (MQ=255)

                                                                                                                     tCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGc                          >  1:1378827/1‑150 (MQ=255)

                                                                                                                      cAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCg                         <  2:329498/150‑1 (MQ=255)

                                                                                                                       agagATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGt                        >  2:1384470/1‑150 (MQ=255)

                                                                                                                       agagATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGt                        >  2:1192939/1‑150 (MQ=255)

                                                                                                                        gagaTGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTg                       <  1:607642/150‑1 (MQ=255)

                                                                                                                                    aTAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGAt           <  1:981467/150‑1 (MQ=255)

                                                                                                                                    aTAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGACTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGAt           >  1:1596528/1‑150 (MQ=255)

                                                                                                                                      aaTGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGAtgc         <  1:1544486/150‑1 (MQ=255)

                                                                                                                                        tGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGAtgctg       >  2:522773/1‑150 (MQ=255)

                                                                                                                                           ggcgCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGATACTCTGGTAGTTGCGTGCCCTCAGTCGATGCTGCAg    >  2:472231/1‑150 (MQ=255)

                                                                                                                                           agcgcAGCCAGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAg    >  2:896803/2‑150 (MQ=255)

                                                                                                                                           agcgcAGCCAGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAg    >  2:1171515/2‑150 (MQ=255)

                                                                                                                                            gcgcAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAgg   <  1:1384470/150‑1 (MQ=255)

                                                                                                                                             cgcAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGc  <  1:563160/150‑1 (MQ=255)

ATGATGATCCACACCGGAGCGTACTCAATGGTGCTTAACATATCGCTGCGAAGTTTTTTCAGCAGGTTCTGGTCTTCTTTACTGACGCCTGGCAGTTTCGCTAGCTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGC  >  NC_000913/3135209‑3135499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`