Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,136,629 C→A 6.0% intergenic (‑258/+34) pitB ← / ← gss phosphate transporter/glutathionylspermidine amidase and glutathionylspermidine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,136,6290CA 6.0% 445.4 / 12.8 159intergenic (‑258/+34)pitB/gssphosphate transporter/glutathionylspermidine amidase and glutathionylspermidine synthetase
Reads supporting (aligned to +/- strand):  ref base C (82/67);  new base A (6/4);  total (88/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.51e-01

Only 100 of 175 total aligned reads displayed.

TTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCT  >  NC_000913/3136480‑3136778
                                                                                                                                                     |                                                                                                                                                     
ttGTGTATCAGACAATATGCATTCATTCAAAACCTTTTGAAATAATCAAAACACAGGTATTCCACTTGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATTTTGAATATTTTGATTATTCCCCGTACTGATTAttc                                                                                                                                                       <  2:379047/150‑1 (MQ=255)
 tgtgTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttct                                                                                                                                                      <  1:1248193/150‑1 (MQ=255)
  gtgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTTGTTAATATATTCACATATGAAATGAATAAATATTTGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttctt                                                                                                                                                     <  2:1376381/150‑1 (MQ=255)
  gtgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttctt                                                                                                                                                     <  1:1095777/150‑1 (MQ=255)
   tgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttcttc                                                                                                                                                    <  2:1629321/150‑1 (MQ=255)
   tgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttcttc                                                                                                                                                    <  1:1132130/150‑1 (MQ=255)
   tgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttcttc                                                                                                                                                    <  1:284259/150‑1 (MQ=255)
   tgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttctta                                                                                                                                                    >  2:1046260/1‑149 (MQ=255)
    gtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCa                                                                                                                                                   <  1:1192548/150‑1 (MQ=255)
    gtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCa                                                                                                                                                   <  2:527823/150‑1 (MQ=255)
     tATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCAt                                                                                                                                                  <  2:1290344/150‑1 (MQ=255)
       tCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATaa                                                                                                                                                >  1:1401590/1‑150 (MQ=255)
         aGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATc                                                                                                                                              >  2:156681/1‑150 (MQ=255)
         aGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATc                                                                                                                                              >  2:345136/1‑150 (MQ=255)
          gACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCa                                                                                                                                             <  1:1520490/150‑1 (MQ=255)
           aCAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAg                                                                                                                                            <  1:557637/150‑1 (MQ=255)
             aaTAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTa                                                                                                                                          <  2:1427228/150‑1 (MQ=255)
             aaTAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTa                                                                                                                                          <  2:1152362/150‑1 (MQ=255)
                 aGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACggg                                                                                                                                      >  1:1013440/1‑150 (MQ=255)
                  gCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATGAAGAATAATCAGTACgggg                                                                                                                                     <  2:1541383/150‑1 (MQ=255)
                   cattcattCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGt                                                                                                                                    <  1:334125/150‑1 (MQ=255)
                    attcattcAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGtt                                                                                                                                   <  2:1365803/150‑1 (MQ=255)
                    attcattcAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGCAATATATAAATATTGAATATTTTGATTAATCCCCTTACTGATTATTCTTCATAATCAGTACGTGGtt                                                                                                                                   >  2:669406/1‑150 (MQ=255)
                     ttcattcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATGAAGAATAATCAGTACggggatt                                                                                                                                  <  2:764561/150‑4 (MQ=39)
                      tcattcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGc                                                                                                                                 <  2:404457/150‑1 (MQ=255)
                      tcattcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGc                                                                                                                                 <  2:1384120/150‑1 (MQ=255)
                       cattcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGTGTTGCt                                                                                                                                >  1:1541996/1‑150 (MQ=255)
                       aattcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCt                                                                                                                                <  2:793198/149‑1 (MQ=255)
                         ttcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTac                                                                                                                              >  1:860782/1‑150 (MQ=255)
                         ttcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTac                                                                                                                              <  1:604838/150‑1 (MQ=255)
                          tcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGTGTTGCTaca                                                                                                                             >  1:261126/1‑150 (MQ=255)
                            ataaCCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTacaac                                                                                                                           <  2:232980/148‑1 (MQ=255)
                             aaaCCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTacaaca                                                                                                                          <  1:1472419/150‑1 (MQ=255)
                               aCCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATg                                                                                                                        <  1:159820/150‑1 (MQ=255)
                                  tttAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCTGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTAt                                                                                                                     >  2:1271898/1‑150 (MQ=255)
                                   ttAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTAtt                                                                                                                    <  2:1506657/150‑1 (MQ=255)
                                      gAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACt                                                                                                                 >  2:1177679/1‑150 (MQ=255)
                                         ataatCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACtttt                                                                                                              <  2:1688885/150‑1 (MQ=255)
                                            atCAAAACACACTTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATAATGGAATATATAAATATTGAATATTTTGATTAAACCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTca                                                                                                           <  1:413178/150‑1 (MQ=255)
                                              cAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTcacc                                                                                                         <  2:538672/150‑1 (MQ=255)
                                               aaaaCACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTcacca                                                                                                        >  2:1130399/1‑150 (MQ=255)
                                               aaaaCACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTcacca                                                                                                        >  1:303913/1‑150 (MQ=255)
                                                 aaCACAGGTATTCCACTGGTTAATATATTAACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACcaca                                                                                                      <  2:220280/150‑1 (MQ=255)
                                                             ccACTGGTTAATATATTCACATATGAATTGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTCATTAATCTTCATAATCAGTACGGGGTTGATACAACATGTATAACTATTTCACAACAATTAACGGttaa                                                                                          >  1:190870/1‑148 (MQ=255)
                                                             ccACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCa                                                                                          >  2:808559/1‑150 (MQ=255)
                                                             ccACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCa                                                                                          >  2:481232/1‑150 (MQ=255)
                                                             ccACTGGTTAATATATTCACAGATGAAATGAATAAATATTGGAATAGATAAATATTGAATATTTTGATTAATCACCGTACTGAGTATTCTTCATTATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCa                                                                                          >  2:259518/1‑150 (MQ=255)
                                                               aCTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAat                                                                                        >  2:1424051/1‑150 (MQ=255)
                                                                cTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAata                                                                                       >  2:1733975/1‑150 (MQ=255)
                                                                cTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAata                                                                                       <  1:481909/150‑1 (MQ=255)
                                                                 tGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTAAGGTGTTGCTACAACATTTATTACTTTTTCACAAAAATTAACGGTTCAatat                                                                                      >  1:317614/1‑150 (MQ=255)
                                                                  ggTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATc                                                                                     <  1:1523616/150‑1 (MQ=255)
                                                                   gTTAATATATTCACATATGAAATGAATAAATATTTGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATAAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCa                                                                                    <  1:508073/150‑1 (MQ=255)
                                                                    ttAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCAc                                                                                   >  1:611652/1‑150 (MQ=255)
                                                                      aaTATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTAATAATAAGTACTGGGTAGCTACAACATGTATTACTTTTTCACCACAATTAACGTTTCAATATCaata                                                                                 >  1:102793/1‑147 (MQ=255)
                                                                         atatTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACTGGTTTGCTAAAACATGTATTACTTTTTCACCACAATTAACTGTTCAATATAActctct                                                                              >  1:101729/1‑150 (MQ=255)
                                                                          tatTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCtt                                                                             >  2:800321/1‑150 (MQ=255)
                                                                              cacaTATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCtttttt                                                                         >  1:551941/1‑150 (MQ=255)
                                                                               acaTATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtg                                                                        >  2:1009998/1‑150 (MQ=255)
                                                                                caTATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtga                                                                       >  1:645882/1‑150 (MQ=255)
                                                                                 atatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCttttt                                                                          <  2:719228/146‑1 (MQ=255)
                                                                                 atatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCttttt                                                                          >  1:719228/1‑146 (MQ=255)
                                                                                 atatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtgat                                                                      >  1:1085413/1‑150 (MQ=255)
                                                                                 atatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtgat                                                                      <  1:1557653/150‑1 (MQ=255)
                                                                                  tatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtgatg                                                                     <  1:622101/150‑1 (MQ=255)
                                                                                    tgaaatgaaTAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGAc                                                                   <  2:1401590/150‑1 (MQ=255)
                                                                                    tgaaatgaaTAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATAACTCTCTTTTTTGATGAc                                                                   >  2:274179/1‑150 (MQ=255)
                                                                                      aaatgaaTAAATATTGGAATATATAAATATTGAATATTTTGATTAATACCCGTACTGATTATTCTTCATAATAAGTAAGGTTTTTCTAAAACATGTATTACTTTATCTCCACAATTAACGGTTCAATATCACTCTCTATTTTTATGAcaa                                                                 >  1:277074/1‑148 (MQ=255)
                                                                                       aatgaaTAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAg                                                                >  2:1123068/1‑150 (MQ=255)
                                                                                         tgaaTAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTg                                                              >  2:583955/1‑150 (MQ=255)
                                                                                           aataaataTTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGAt                                                            <  2:303913/150‑1 (MQ=255)
                                                                                              aaataTTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATtca                                                         <  2:1605824/150‑1 (MQ=255)
                                                                                              aaataTTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATtca                                                         <  1:1416565/150‑1 (MQ=255)
                                                                                                        atatatAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCa                                               <  1:177627/150‑1 (MQ=255)
                                                                                                           tataAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAac                                            <  2:1541996/150‑1 (MQ=255)
                                                                                                             taAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAacac                                          <  1:830647/150‑1 (MQ=255)
                                                                                                                  atTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTTAGGGGTTGATACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACAGCGCAAACACGTccc                                     >  1:116149/1‑150 (MQ=255)
                                                                                                                     gAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCAcc                                  >  2:1714542/1‑150 (MQ=255)
                                                                                                                         atTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCCCCGTAg                              >  2:1266684/1‑150 (MQ=255)
                                                                                                                          ttttGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATAACCGCGCAAACACGTCCCACCGTAGt                             >  2:1342133/1‑150 (MQ=255)
                                                                                                                            ttGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTg                           >  2:400642/1‑150 (MQ=255)
                                                                                                                            ttGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACAAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTg                           >  1:1529021/1‑150 (MQ=255)
                                                                                                                             tGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTAgc                          >  2:427639/1‑150 (MQ=255)
                                                                                                                                  aaTCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCa                     >  2:1078440/1‑150 (MQ=255)
                                                                                                                                   aaccccGTACTGATTATGAAGAATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCaa                    >  1:1062647/3‑150 (MQ=39)
                                                                                                                                    accccGTACAGATTATTCTTCATAATCAGTAAGGGGTTGCTACAACATGTATTACTTAATCACCACAATTACCGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAAc                   <  1:1681960/149‑1 (MQ=255)
                                                                                                                                      cccGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACgg                 >  1:454150/1‑150 (MQ=255)
                                                                                                                                      cccGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACgg                 >  1:940353/1‑150 (MQ=255)
                                                                                                                                      cccGTACTGATTATTCTTCATAATCAGTACGGGGTTGCAACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACgg                 >  2:656708/1‑150 (MQ=255)
                                                                                                                                      cccGTACTGATTATGAAGAATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACgg                 >  2:299613/1‑150 (MQ=255)
                                                                                                                                        cttACTGATTATTCTTGATAATCAGTACGGGGTTGCTACTACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTTCCGCCAACGGTg               <  1:669406/148‑1 (MQ=255)
                                                                                                                                        cGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTg               >  1:593253/1‑150 (MQ=255)
                                                                                                                                         gTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGa              >  2:1010170/1‑150 (MQ=255)
                                                                                                                                            cTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAgg           <  1:274179/150‑1 (MQ=255)
                                                                                                                                             tGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGt          <  1:525170/150‑1 (MQ=255)
                                                                                                                                                ttattCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACa       <  1:476473/150‑1 (MQ=255)
                                                                                                                                                 tattCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACAt      >  1:228188/1‑150 (MQ=255)
                                                                                                                                                  attCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATa     >  2:1529030/1‑150 (MQ=255)
                                                                                                                                                    tcttcATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATAcc   <  2:645658/150‑1 (MQ=255)
                                                                                                                                                     cttcATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCt  >  2:970223/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCT  >  NC_000913/3136480‑3136778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: