Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,136,630 T→A 5.7% intergenic (‑259/+33) pitB ← / ← gss phosphate transporter/glutathionylspermidine amidase and glutathionylspermidine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,136,6300TA 5.7% 445.5 / 11.1 159intergenic (‑259/+33)pitB/gssphosphate transporter/glutathionylspermidine amidase and glutathionylspermidine synthetase
Reads supporting (aligned to +/- strand):  ref base T (83/67);  new base A (5/4);  total (88/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01

Only 100 of 176 total aligned reads displayed.

TGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTG  >  NC_000913/3136481‑3136779
                                                                                                                                                     |                                                                                                                                                      
tgtgTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttct                                                                                                                                                        <  1:1248193/150‑1 (MQ=255)
tgtgTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttct                                                                                                                                                        <  2:106387/150‑1 (MQ=255)
 gtgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTTGTTAATATATTCACATATGAAATGAATAAATATTTGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttctt                                                                                                                                                       <  2:1376381/150‑1 (MQ=255)
 gtgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttctt                                                                                                                                                       <  1:1095777/150‑1 (MQ=255)
  tgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttcttc                                                                                                                                                      <  2:245824/150‑1 (MQ=255)
  tgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttcttc                                                                                                                                                      <  2:1629321/150‑1 (MQ=255)
  tgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttcttc                                                                                                                                                      <  1:1132130/150‑1 (MQ=255)
  tgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttctta                                                                                                                                                      >  2:1046260/1‑149 (MQ=255)
   gtATCAGACAATAATCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCa                                                                                                                                                     <  1:609405/150‑1 (MQ=255)
   gtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCa                                                                                                                                                     <  1:1192548/150‑1 (MQ=255)
    tATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCAt                                                                                                                                                    <  2:1290344/150‑1 (MQ=255)
     aTCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATa                                                                                                                                                   >  1:1520897/1‑150 (MQ=255)
        aGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAAta                                                                                                                                                >  2:508073/1‑149 (MQ=255)
        aGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATc                                                                                                                                                >  2:156681/1‑150 (MQ=255)
         gACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCa                                                                                                                                               >  2:1717742/1‑150 (MQ=255)
          aCAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAg                                                                                                                                              <  1:557637/150‑1 (MQ=255)
           cAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGt                                                                                                                                             >  2:382143/1‑150 (MQ=255)
            aaaaaGCATTCATTCAAAACCTTTACAAATAAACAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATAAATAAATATTGAATATTTTTATTAAACCCCTTACTGATTATTCTTCATAATCAGTa                                                                                                                                            >  2:777429/4‑150 (MQ=255)
            aaTAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTa                                                                                                                                            <  2:1427228/150‑1 (MQ=255)
            aaTAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTa                                                                                                                                            <  2:1152362/150‑1 (MQ=255)
              tAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACg                                                                                                                                          >  1:1388544/1‑150 (MQ=255)
                aGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACggg                                                                                                                                        >  1:1013440/1‑150 (MQ=255)
                aGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACggg                                                                                                                                        >  1:645658/1‑150 (MQ=255)
                aGCATTCATTCAAAACCTTTAGAAAAAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATGAAGAATAATCAGTACggg                                                                                                                                        <  2:347441/150‑1 (MQ=255)
                  cattcattCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGt                                                                                                                                      <  1:334125/150‑1 (MQ=255)
                   attcattcAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGCAATATATAAATATTGAATATTTTGATTAATCCCCTTACTGATTATTCTTCATAATCAGTACGTGGtt                                                                                                                                     >  2:669406/1‑150 (MQ=255)
                   attcattaAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATGAAGAATAATCAGTACggggat                                                                                                                                     <  2:125480/150‑3 (MQ=39)
                    ttcattcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATGAAGAATAATCAGTACggggatt                                                                                                                                    <  2:764561/150‑4 (MQ=39)
                     tcattcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGc                                                                                                                                   <  2:1384120/150‑1 (MQ=255)
                     tcattcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGc                                                                                                                                   <  2:1520897/150‑1 (MQ=255)
                     tcattcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGc                                                                                                                                   <  2:404457/150‑1 (MQ=255)
                      cattcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGTGTTGCt                                                                                                                                  >  1:1541996/1‑150 (MQ=255)
                      cattcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCt                                                                                                                                  >  2:1536037/1‑150 (MQ=255)
                      aattcaAAACCTTTAGAAATAATCAAAACACATTTATTCCACTGTTTAATATATTCACATATGAAATGCATAAATATTGGAATATATAATTATGGTCTATTTTGATTAATCCCCGTACTGATTAGTCTTCATAATCAGTACGGGGTTTCt                                                                                                                                  <  1:280474/149‑1 (MQ=255)
                       attcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCACCGTACTGATTATTCTTAATAATCAGTACGGGGTTGCTa                                                                                                                                 >  1:575779/1‑150 (MQ=255)
                           aaaaCCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTacaac                                                                                                                             <  1:1526569/150‑1 (MQ=255)
                           aaaaCCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACAAATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTacaac                                                                                                                             <  2:312630/150‑1 (MQ=255)
                            aaaCCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTacaaca                                                                                                                            <  1:1472419/150‑1 (MQ=255)
                              aCCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATg                                                                                                                          <  2:1423438/150‑1 (MQ=255)
                                 tttAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCTGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTAt                                                                                                                       >  2:1271898/1‑150 (MQ=255)
                                  ttAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTAtt                                                                                                                      <  2:1506657/150‑1 (MQ=255)
                                     gAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACt                                                                                                                  <  2:651889/150‑1 (MQ=255)
                                             cAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTcacc                                                                                                          <  2:538672/150‑1 (MQ=255)
                                             cAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTcaac                                                                                                          >  2:1701141/1‑148 (MQ=255)
                                              aaaaCACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATTTATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTcacca                                                                                                         >  2:1172876/1‑150 (MQ=255)
                                              aaaaCACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTcacca                                                                                                         >  2:1130399/1‑150 (MQ=255)
                                                aaCACAGGTATTCCACTGGTTAATATATTAACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACcaca                                                                                                       <  2:220280/150‑1 (MQ=255)
                                                      ggTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAAc                                                                                                 <  1:993086/150‑1 (MQ=255)
                                                       gTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACg                                                                                                <  1:465742/150‑1 (MQ=255)
                                                         aTTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTTCGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAAAGGt                                                                                              >  1:268859/1‑150 (MQ=255)
                                                            ccACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCa                                                                                           >  2:481232/1‑150 (MQ=255)
                                                            ccACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCa                                                                                           >  2:808559/1‑150 (MQ=255)
                                                              aCTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAat                                                                                         <  1:859535/150‑1 (MQ=255)
                                                               cTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAata                                                                                        <  1:481909/150‑1 (MQ=255)
                                                               cTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAata                                                                                        >  2:1733975/1‑150 (MQ=255)
                                                                  gTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCa                                                                                     <  1:597455/150‑1 (MQ=255)
                                                                   ttAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCAc                                                                                    <  1:1728107/150‑1 (MQ=255)
                                                                     aaTATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTAATAATAAGTACTGGGTAGCTACAACATGTATTACTTTTTCACCACAATTAACGTTTCAATATCaata                                                                                  >  1:102793/1‑147 (MQ=255)
                                                                       tatatTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCActctc                                                                                <  1:38041/150‑1 (MQ=255)
                                                                        atatTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACTGGTTTGCTAAAACATGTATTACTTTTTCACCACAATTAACTGTTCAATATAActctct                                                                               >  1:101729/1‑150 (MQ=255)
                                                                         tatTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCtt                                                                              >  2:800321/1‑150 (MQ=255)
                                                                                atatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCttttt                                                                           <  2:719228/146‑1 (MQ=255)
                                                                                atatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtgat                                                                       <  1:1417986/150‑1 (MQ=255)
                                                                                atatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtgat                                                                       <  1:288078/150‑1 (MQ=255)
                                                                                atatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtgat                                                                       >  1:1085413/1‑150 (MQ=255)
                                                                                 tatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtgatg                                                                      <  1:622101/150‑1 (MQ=255)
                                                                                   tgaaatgaaTAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATAACTCTCTTTTTTGATGAc                                                                    >  2:274179/1‑150 (MQ=255)
                                                                                    gaaatgaaTAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTATTTTGATGAca                                                                   >  1:705069/1‑149 (MQ=255)
                                                                                     aaatgaaTAAATATTGGAATATATAAATATTGAATATTTTGATTAATACCCGTACTGATTATTCTTCATAATAAGTAAGGTTTTTCTAAAACATGTATTACTTTATCTCCACAATTAACGGTTCAATATCACTCTCTATTTTTATGAcaa                                                                  >  1:277074/1‑148 (MQ=255)
                                                                                        tgaaTAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTg                                                               >  2:583955/1‑150 (MQ=255)
                                                                                           ataaataTTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGAtt                                                            >  1:1563989/1‑150 (MQ=255)
                                                                                           ataaataTTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGAtt                                                            <  1:209052/150‑1 (MQ=255)
                                                                                             aaataTTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATtca                                                          <  2:1605824/150‑1 (MQ=255)
                                                                                                  ttGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCAcc                                                     >  2:1345913/1‑150 (MQ=255)
                                                                                                   tGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACcg                                                    <  2:1121034/150‑1 (MQ=255)
                                                                                                    ggAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACcgc                                                   >  1:903037/1‑150 (MQ=255)
                                                                                                       atatatAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCa                                                <  1:177627/150‑1 (MQ=255)
                                                                                                          tataAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAac                                             <  2:1541996/150‑1 (MQ=255)
                                                                                                            taAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAacac                                           <  1:830647/150‑1 (MQ=255)
                                                                                                                 atTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTTAGGGGTTGATACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACAGCGCAAACACGTccc                                      >  1:116149/1‑150 (MQ=255)
                                                                                                                       tatTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTa                                <  1:537934/150‑1 (MQ=255)
                                                                                                                       tatTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACAAGTGATTCATCACCTCGCAAACACGTCCCACCGTa                                >  1:31412/1‑150 (MQ=255)
                                                                                                                            tGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTAgc                           >  2:427639/1‑150 (MQ=255)
                                                                                                                              aTTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTgccg                         >  1:661156/1‑150 (MQ=255)
                                                                                                                              aTTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTgccg                         >  2:1393178/1‑150 (MQ=255)
                                                                                                                                  aaccccGTACTGATTATGAAGAATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCaa                     >  1:1062647/3‑150 (MQ=39)
                                                                                                                                  aTCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCaa                     >  2:1305730/1‑150 (MQ=255)
                                                                                                                                     cccGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACgg                  <  1:1536037/150‑1 (MQ=255)
                                                                                                                                     cccGTACTGATTATGAAGAATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACgg                  >  2:299613/1‑150 (MQ=255)
                                                                                                                                       cGTACTGATTATGAAGAATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTg                >  1:1680813/1‑150 (MQ=255)
                                                                                                                                        gTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGa               >  2:1010170/1‑150 (MQ=255)
                                                                                                                                          aCTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAg             <  1:1412569/150‑1 (MQ=255)
                                                                                                                                          aCTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTAATACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAg             <  1:500160/150‑1 (MQ=255)
                                                                                                                                             gATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTa          >  1:428502/1‑150 (MQ=255)
                                                                                                                                              attattCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTa          <  1:229693/150‑1 (MQ=255)
                                                                                                                                                 attCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATa      >  1:512540/1‑150 (MQ=255)
                                                                                                                                                   tcttcATAATCAGTACTGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATAcc    <  2:267127/150‑1 (MQ=255)
                                                                                                                                                   tcttcATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATAcc    <  2:645658/150‑1 (MQ=255)
                                                                                                                                                    cttcATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCt   >  2:970223/1‑150 (MQ=255)
                                                                                                                                                     ttcATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTg  <  2:1563989/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTG  >  NC_000913/3136481‑3136779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: