Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,136,631 T→G 5.7% intergenic (‑260/+32) pitB ← / ← gss phosphate transporter/glutathionylspermidine amidase and glutathionylspermidine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,136,6310TG 5.7% 490.7 / 14.9 159intergenic (‑260/+32)pitB/gssphosphate transporter/glutathionylspermidine amidase and glutathionylspermidine synthetase
Reads supporting (aligned to +/- strand):  ref base T (83/67);  new base G (5/4);  total (88/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-01

Only 100 of 176 total aligned reads displayed.

GTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGA  >  NC_000913/3136482‑3136780
                                                                                                                                                     |                                                                                                                                                     
gtgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTTGTTAATATATTCACATATGAAATGAATAAATATTTGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttctt                                                                                                                                                       <  2:1376381/150‑1 (MQ=255)
gtgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttctt                                                                                                                                                       <  1:1095777/150‑1 (MQ=255)
 tgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttcttc                                                                                                                                                      <  2:245824/150‑1 (MQ=255)
 tgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttcttc                                                                                                                                                      <  2:1629321/150‑1 (MQ=255)
 tgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttcttc                                                                                                                                                      <  1:284259/150‑1 (MQ=255)
 tgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttcttc                                                                                                                                                      <  1:1132130/150‑1 (MQ=255)
 tgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttctta                                                                                                                                                      >  2:1046260/1‑149 (MQ=255)
  gtATCAGACAATAATCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCa                                                                                                                                                     <  1:609405/150‑1 (MQ=255)
  gtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCa                                                                                                                                                     <  1:1192548/150‑1 (MQ=255)
   tATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCAt                                                                                                                                                    <  2:1290344/150‑1 (MQ=255)
     tCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATaa                                                                                                                                                  >  1:1401590/1‑150 (MQ=255)
       aGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAAta                                                                                                                                                >  2:508073/1‑149 (MQ=255)
       aGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATc                                                                                                                                                >  2:156681/1‑150 (MQ=255)
        gACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCa                                                                                                                                               <  1:1520490/150‑1 (MQ=255)
        gACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCa                                                                                                                                               >  2:1717742/1‑150 (MQ=255)
         aCAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAg                                                                                                                                              <  1:557637/150‑1 (MQ=255)
          cAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGt                                                                                                                                             >  2:382143/1‑150 (MQ=255)
           aaaaaGCATTCATTCAAAACCTTTACAAATAAACAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATAAATAAATATTGAATATTTTTATTAAACCCCTTACTGATTATTCTTCATAATCAGTa                                                                                                                                            >  2:777429/4‑150 (MQ=255)
           aaTAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTa                                                                                                                                            <  2:1152362/150‑1 (MQ=255)
           aaTAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTa                                                                                                                                            <  2:1427228/150‑1 (MQ=255)
               aGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACggg                                                                                                                                        >  1:645658/1‑150 (MQ=255)
               aGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACggg                                                                                                                                        >  1:1013440/1‑150 (MQ=255)
               aGCATTCATTCAAAACCTTTAGAAAAAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATGAAGAATAATCAGTACggg                                                                                                                                        <  2:347441/150‑1 (MQ=255)
                  attcattcAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGCAATATATAAATATTGAATATTTTGATTAATCCCCTTACTGATTATTCTTCATAATCAGTACGTGGtt                                                                                                                                     >  2:669406/1‑150 (MQ=255)
                  attcattaAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATGAAGAATAATCAGTACggggat                                                                                                                                     <  2:125480/150‑3 (MQ=39)
                   ttcattcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATGAAGAATAATCAGTACggggatt                                                                                                                                    <  2:764561/150‑4 (MQ=39)
                    tcattcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGc                                                                                                                                   <  2:404457/150‑1 (MQ=255)
                    tcattcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGc                                                                                                                                   <  2:1520897/150‑1 (MQ=255)
                    tcattcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGc                                                                                                                                   <  2:1384120/150‑1 (MQ=255)
                     cattcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGTGTTGCt                                                                                                                                  >  1:1541996/1‑150 (MQ=255)
                     cattcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCt                                                                                                                                  >  2:1536037/1‑150 (MQ=255)
                     aattcaAAACCTTTAGAAATAATCAAAACACATTTATTCCACTGTTTAATATATTCACATATGAAATGCATAAATATTGGAATATATAATTATGGTCTATTTTGATTAATCCCCGTACTGATTAGTCTTCATAATCAGTACGGGGTTTCt                                                                                                                                  <  1:280474/149‑1 (MQ=255)
                      attcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCACCGTACTGATTATTCTTAATAATCAGTACGGGGTTGCTa                                                                                                                                 >  1:575779/1‑150 (MQ=255)
                        tcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGTGTTGCTaca                                                                                                                               >  1:261126/1‑150 (MQ=255)
                          aaaaCCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACAAATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTacaac                                                                                                                             <  2:312630/150‑1 (MQ=255)
                           aaaCCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTacaaca                                                                                                                            <  1:1472419/150‑1 (MQ=255)
                             aCCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATg                                                                                                                          <  1:159820/150‑1 (MQ=255)
                             aCCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATg                                                                                                                          <  2:1423438/150‑1 (MQ=255)
                                tttAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCTGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTAt                                                                                                                       >  2:1271898/1‑150 (MQ=255)
                                 ttAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTAtt                                                                                                                      <  2:1506657/150‑1 (MQ=255)
                                    gAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACt                                                                                                                   <  2:651889/150‑1 (MQ=255)
                                          atCAAAACACACTTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATAATGGAATATATAAATATTGAATATTTTGATTAAACCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTca                                                                                                             <  1:413178/150‑1 (MQ=255)
                                            cAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTcacc                                                                                                           <  2:538672/150‑1 (MQ=255)
                                            cAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTcaac                                                                                                           >  2:1701141/1‑148 (MQ=255)
                                             aaaaCACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATTTATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTcacca                                                                                                          >  2:1172876/1‑150 (MQ=255)
                                             aaaaCACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTcacca                                                                                                          >  2:1130399/1‑150 (MQ=255)
                                             aaaaCACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTcacca                                                                                                          >  1:303913/1‑150 (MQ=255)
                                               aaCACAGGTATTCCACTGGTTAATATATTAACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACcaca                                                                                                        <  2:220280/150‑1 (MQ=255)
                                                      gTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACg                                                                                                 <  1:465742/150‑1 (MQ=255)
                                                           ccACTGGTTAATATATTCACATATGAATTGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTCATTAATCTTCATAATCAGTACGGGGTTGATACAACATGTATAACTATTTCACAACAATTAACGGttaa                                                                                            >  1:190870/1‑148 (MQ=255)
                                                           ccACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCa                                                                                            >  2:481232/1‑150 (MQ=255)
                                                           ccACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCa                                                                                            >  2:808559/1‑150 (MQ=255)
                                                             aCTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAat                                                                                          <  1:859535/150‑1 (MQ=255)
                                                              cTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAata                                                                                         >  2:1733975/1‑150 (MQ=255)
                                                              cTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAata                                                                                         <  1:481909/150‑1 (MQ=255)
                                                               tGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTAAGGTGTTGCTACAACATTTATTACTTTTTCACAAAAATTAACGGTTCAatat                                                                                        >  1:317614/1‑150 (MQ=255)
                                                                ggTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATc                                                                                       <  1:1523616/150‑1 (MQ=255)
                                                                 gTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCa                                                                                      <  1:597455/150‑1 (MQ=255)
                                                                    aaTATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTAATAATAAGTACTGGGTAGCTACAACATGTATTACTTTTTCACCACAATTAACGTTTCAATATCaata                                                                                   >  1:102793/1‑147 (MQ=255)
                                                                      tatatTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCActctc                                                                                 <  1:38041/150‑1 (MQ=255)
                                                                       atatTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACTGGTTTGCTAAAACATGTATTACTTTTTCACCACAATTAACTGTTCAATATAActctct                                                                                >  1:101729/1‑150 (MQ=255)
                                                                        tatTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCtt                                                                               >  2:800321/1‑150 (MQ=255)
                                                                             acaTATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtg                                                                          >  2:1009998/1‑150 (MQ=255)
                                                                               atatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCttttt                                                                            <  2:719228/146‑1 (MQ=255)
                                                                               atatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtgat                                                                        <  1:1557653/150‑1 (MQ=255)
                                                                               atatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtgat                                                                        >  1:1085413/1‑150 (MQ=255)
                                                                                tatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtgatg                                                                       <  1:622101/150‑1 (MQ=255)
                                                                                  tgaaatgaaTAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATAACTCTCTTTTTTGATGAc                                                                     >  2:274179/1‑150 (MQ=255)
                                                                                   gaaatgaaTAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTATTTTGATGAca                                                                    >  1:705069/1‑149 (MQ=255)
                                                                                       tgaaTAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTg                                                                >  2:583955/1‑150 (MQ=255)
                                                                                            aaataTTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATtca                                                           <  1:1416565/150‑1 (MQ=255)
                                                                                            aaataTTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATtca                                                           <  2:1605824/150‑1 (MQ=255)
                                                                                                 ttGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCAcc                                                      >  2:1345913/1‑150 (MQ=255)
                                                                                                  tGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACcg                                                     <  2:1121034/150‑1 (MQ=255)
                                                                                                      atatatAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCa                                                 <  1:177627/150‑1 (MQ=255)
                                                                                                         tataAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAac                                              <  2:1541996/150‑1 (MQ=255)
                                                                                                           taAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAacac                                            <  1:830647/150‑1 (MQ=255)
                                                                                                                atTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTTAGGGGTTGATACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACAGCGCAAACACGTccc                                       >  1:116149/1‑150 (MQ=255)
                                                                                                                      tatTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTa                                 <  1:537934/150‑1 (MQ=255)
                                                                                                                          ttGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACAAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTg                             >  1:1529021/1‑150 (MQ=255)
                                                                                                                           tGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTAgc                            >  2:427639/1‑150 (MQ=255)
                                                                                                                             aTTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTgccg                          >  2:1393178/1‑150 (MQ=255)
                                                                                                                             aTTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTgccg                          >  1:661156/1‑150 (MQ=255)
                                                                                                                                 aaccccGTACTGATTATGAAGAATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCaa                      >  1:1062647/3‑150 (MQ=39)
                                                                                                                                 aTCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCaa                      >  2:1305730/1‑150 (MQ=255)
                                                                                                                                  accccGTACAGATTATTCTTCATAATCAGTAAGGGGTTGCTACAACATGTATTACTTAATCACCACAATTACCGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAAc                     <  1:1681960/149‑1 (MQ=255)
                                                                                                                                    cccGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACgg                   >  1:940353/1‑150 (MQ=255)
                                                                                                                                    cccGTACTGATTATGAAGAATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACgg                   >  2:299613/1‑150 (MQ=255)
                                                                                                                                         aCTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTAATACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAg              <  1:500160/150‑1 (MQ=255)
                                                                                                                                          cTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAgg             <  1:274179/150‑1 (MQ=255)
                                                                                                                                            gATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTa           >  1:428502/1‑150 (MQ=255)
                                                                                                                                               tattCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACAt        >  1:228188/1‑150 (MQ=255)
                                                                                                                                                attCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATa       >  1:512540/1‑150 (MQ=255)
                                                                                                                                                  tcttcATAATCAGTACTGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATAcc     <  2:267127/150‑1 (MQ=255)
                                                                                                                                                  tcttcATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATAcc     <  2:1013440/150‑1 (MQ=255)
                                                                                                                                                  tcttcATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATAcc     <  2:645658/150‑1 (MQ=255)
                                                                                                                                                   cttcATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCt    >  2:970223/1‑150 (MQ=255)
                                                                                                                                                    ttcATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTg   <  2:1563989/150‑1 (MQ=255)
                                                                                                                                                    ttcATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCAATCTCTTTTTTGATGACCAGTGATTCATCACCGCGCCAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCt  >  1:1267310/1‑149 (MQ=255)
                                                                                                                                                     tcATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGa  >  1:869629/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGA  >  NC_000913/3136482‑3136780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: