Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,136,632 C→A 6.3% intergenic (‑261/+31) pitB ← / ← gss phosphate transporter/glutathionylspermidine amidase and glutathionylspermidine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,136,6320CA 6.3% 442.0 / 13.6 159intergenic (‑261/+31)pitB/gssphosphate transporter/glutathionylspermidine amidase and glutathionylspermidine synthetase
Reads supporting (aligned to +/- strand):  ref base C (81/66);  new base A (7/4);  total (88/71)
Fisher's exact test for biased strand distribution p-value = 7.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.20e-01

Only 100 of 173 total aligned reads displayed.

TGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGA  >  NC_000913/3136483‑3136780
                                                                                                                                                     |                                                                                                                                                     
tgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttcttc                                                                                                                                                       <  2:427516/150‑1 (MQ=255)
tgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttcttc                                                                                                                                                       <  1:284259/150‑1 (MQ=255)
tgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttcttc                                                                                                                                                       <  2:245824/150‑1 (MQ=255)
 gtATCAGACAATAATCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCa                                                                                                                                                      <  1:609405/150‑1 (MQ=255)
 gtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCa                                                                                                                                                      <  1:1192548/150‑1 (MQ=255)
 gtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCa                                                                                                                                                      <  2:527823/150‑1 (MQ=255)
  tATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCAt                                                                                                                                                     <  2:1290344/150‑1 (MQ=255)
   aTCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATa                                                                                                                                                    >  1:1520897/1‑150 (MQ=255)
      aGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATc                                                                                                                                                 >  2:156681/1‑150 (MQ=255)
       gACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCa                                                                                                                                                >  2:1717742/1‑150 (MQ=255)
        aCAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAg                                                                                                                                               <  1:557637/150‑1 (MQ=255)
          aaTAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTa                                                                                                                                             <  2:1152362/150‑1 (MQ=255)
            tAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACg                                                                                                                                           >  1:1388544/1‑150 (MQ=255)
              aGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACggg                                                                                                                                         <  2:27824/150‑1 (MQ=255)
              aGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACggg                                                                                                                                         >  1:645658/1‑150 (MQ=255)
              aGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACggg                                                                                                                                         >  1:1013440/1‑150 (MQ=255)
              aGCATTCATTCAAAACCTTTAGAAAAAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATGAAGAATAATCAGTACggg                                                                                                                                         <  2:347441/150‑1 (MQ=255)
               gCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATGAAGAATAATCAGTACgggg                                                                                                                                        <  2:1541383/150‑1 (MQ=255)
                 attcattcAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGCAATATATAAATATTGAATATTTTGATTAATCCCCTTACTGATTATTCTTCATAATCAGTACGTGGtt                                                                                                                                      >  2:669406/1‑150 (MQ=255)
                 attcattaAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATGAAGAATAATCAGTACggggat                                                                                                                                      <  2:125480/150‑3 (MQ=39)
                  ttcattcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATGAAGAATAATCAGTACggggatt                                                                                                                                     <  2:764561/150‑4 (MQ=39)
                   tcattcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGc                                                                                                                                    <  2:1520897/150‑1 (MQ=255)
                    cattcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGTGTTGCt                                                                                                                                   >  1:1541996/1‑150 (MQ=255)
                    aattcaAAACCTTTAGAAATAATCAAAACACATTTATTCCACTGTTTAATATATTCACATATGAAATGCATAAATATTGGAATATATAATTATGGTCTATTTTGATTAATCCCCGTACTGATTAGTCTTCATAATCAGTACGGGGTTTCt                                                                                                                                   <  1:280474/149‑1 (MQ=255)
                    aattcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCt                                                                                                                                   <  2:793198/149‑1 (MQ=255)
                     attcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCACCGTACTGATTATTCTTAATAATCAGTACGGGGTTGCTa                                                                                                                                  >  1:575779/1‑150 (MQ=255)
                       tcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGTGTTGCTaca                                                                                                                                >  1:261126/1‑150 (MQ=255)
                         ataaCCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTacaac                                                                                                                              <  2:232980/148‑1 (MQ=255)
                         aaaaCCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTacaac                                                                                                                              <  1:1526569/150‑1 (MQ=255)
                         aaaaCCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACAAATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTacaac                                                                                                                              <  2:312630/150‑1 (MQ=255)
                          aaaCCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTacaaca                                                                                                                             <  1:1472419/150‑1 (MQ=255)
                           aaCCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACAt                                                                                                                            <  2:1549817/150‑1 (MQ=255)
                               tttAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCTGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTAt                                                                                                                        >  2:1271898/1‑150 (MQ=255)
                                   gAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACt                                                                                                                   >  2:1177679/1‑150 (MQ=255)
                                      ataatCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACtttt                                                                                                                <  2:1688885/150‑1 (MQ=255)
                                         atCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTca                                                                                                             >  2:209052/1‑150 (MQ=255)
                                         atCAAAACACACTTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATAATGGAATATATAAATATTGAATATTTTGATTAAACCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTca                                                                                                             <  1:413178/150‑1 (MQ=255)
                                           cAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTcacc                                                                                                           <  2:538672/150‑1 (MQ=255)
                                            aaaaCACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTcacca                                                                                                          >  2:1130399/1‑150 (MQ=255)
                                            aaaaCACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTcacca                                                                                                          >  1:303913/1‑150 (MQ=255)
                                             aaaCACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTcaccac                                                                                                         >  2:1582308/1‑150 (MQ=255)
                                                    ggTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAAc                                                                                                  <  1:993086/150‑1 (MQ=255)
                                                     gTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACg                                                                                                 <  1:465742/150‑1 (MQ=255)
                                                          ccACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCa                                                                                            >  2:1622474/1‑150 (MQ=255)
                                                          ccACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCa                                                                                            >  2:808559/1‑150 (MQ=255)
                                                          ccACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCa                                                                                            >  2:481232/1‑150 (MQ=255)
                                                            aCTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAat                                                                                          >  2:1424051/1‑150 (MQ=255)
                                                            aCTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAat                                                                                          <  1:859535/150‑1 (MQ=255)
                                                             cTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAata                                                                                         <  1:481909/150‑1 (MQ=255)
                                                              tGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTAAGGTGTTGCTACAACATTTATTACTTTTTCACAAAAATTAACGGTTCAatat                                                                                        >  1:317614/1‑150 (MQ=255)
                                                                gTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCa                                                                                      <  1:597455/150‑1 (MQ=255)
                                                                 ttAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCAc                                                                                     >  1:611652/1‑150 (MQ=255)
                                                                   aaTATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTAATAATAAGTACTGGGTAGCTACAACATGTATTACTTTTTCACCACAATTAACGTTTCAATATCaata                                                                                   >  1:102793/1‑147 (MQ=255)
                                                                     tatatTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCActctc                                                                                 <  1:38041/150‑1 (MQ=255)
                                                                      atatTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACTGGTTTGCTAAAACATGTATTACTTTTTCACCACAATTAACTGTTCAATATAActctct                                                                                >  1:101729/1‑150 (MQ=255)
                                                                      atatTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCAGTACTGATTATTCTTCATAATCAGTACTGGGTTGCTACAAAATGTATTAATTTTTCAACACAAATAACGGTTCAATATCActctct                                                                                >  1:106266/1‑150 (MQ=255)
                                                                       tatTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCtt                                                                               >  2:800321/1‑150 (MQ=255)
                                                                            acaTATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtg                                                                          <  2:1496436/150‑1 (MQ=255)
                                                                              atatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCttttt                                                                            >  1:719228/1‑146 (MQ=255)
                                                                              atatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCttttt                                                                            <  2:719228/146‑1 (MQ=255)
                                                                              atatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtgat                                                                        <  1:1417986/150‑1 (MQ=255)
                                                                               tatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCTGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtgatg                                                                       >  1:1141160/1‑150 (MQ=255)
                                                                                 tgaaatgaaTAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGAc                                                                     <  2:1401590/150‑1 (MQ=255)
                                                                                  gaaatgaaTAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTATTTTGATGAca                                                                    >  1:705069/1‑149 (MQ=255)
                                                                                      tgaaTAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTg                                                                >  2:583955/1‑150 (MQ=255)
                                                                                        aataaataTTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGAt                                                              >  2:1380474/1‑150 (MQ=255)
                                                                                        aataaataTTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGAt                                                              <  2:303913/150‑1 (MQ=255)
                                                                                         ataaataTTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGAtt                                                             >  1:1563989/1‑150 (MQ=255)
                                                                                         ataaataTTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGAtt                                                             <  1:209052/150‑1 (MQ=255)
                                                                                                ttGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCAcc                                                      >  2:1345913/1‑150 (MQ=255)
                                                                                                 tGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACcg                                                     <  2:1121034/150‑1 (MQ=255)
                                                                                                     atatatAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCa                                                 <  1:177627/150‑1 (MQ=255)
                                                                                                                  gAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCAcc                                    >  2:1714542/1‑150 (MQ=255)
                                                                                                                     tatTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTa                                 <  1:537934/150‑1 (MQ=255)
                                                                                                                       ttttGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATAACCGCGCAAACACGTCCCACCGTAGt                               >  2:1342133/1‑150 (MQ=255)
                                                                                                                         ttGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTg                             >  2:400642/1‑150 (MQ=255)
                                                                                                                         ttGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACAAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTg                             >  1:1529021/1‑150 (MQ=255)
                                                                                                                          tGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTAgc                            >  2:427639/1‑150 (MQ=255)
                                                                                                                            aTTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTgccg                          >  1:661156/1‑150 (MQ=255)
                                                                                                                            aTTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTgccg                          >  2:1393178/1‑150 (MQ=255)
                                                                                                                               aaTCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCa                       >  2:1078440/1‑150 (MQ=255)
                                                                                                                                aaccccGTACTGATTATGAAGAATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCaa                      >  1:1062647/3‑150 (MQ=39)
                                                                                                                                 accccGTACAGATTATTCTTCATAATCAGTAAGGGGTTGCTACAACATGTATTACTTAATCACCACAATTACCGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAAc                     <  1:1681960/149‑1 (MQ=255)
                                                                                                                                   cccGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACgg                   >  1:940353/1‑150 (MQ=255)
                                                                                                                                   cccGTACTGATTATTCTTCATAATCAGTACGGGGTTGCAACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACgg                   >  2:656708/1‑150 (MQ=255)
                                                                                                                                   cccGTACTGATTATGAAGAATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACgg                   >  1:1122476/1‑150 (MQ=255)
                                                                                                                                     cGTACTGATTATGAAGAATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTg                 >  1:1680813/1‑150 (MQ=255)
                                                                                                                                      gTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGa                >  2:1010170/1‑150 (MQ=255)
                                                                                                                                        aCTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAg              <  1:1412569/150‑1 (MQ=255)
                                                                                                                                        aCTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTAATACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAg              <  1:500160/150‑1 (MQ=255)
                                                                                                                                         cTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAgg             <  1:274179/150‑1 (MQ=255)
                                                                                                                                           gATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTa           >  1:428502/1‑150 (MQ=255)
                                                                                                                                            attattCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTa           <  1:229693/150‑1 (MQ=255)
                                                                                                                                               attCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATa       >  2:1529030/1‑150 (MQ=255)
                                                                                                                                               attCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATa       >  1:512540/1‑150 (MQ=255)
                                                                                                                                                 tcttcATAATCAGTACTGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATAcc     <  2:267127/150‑1 (MQ=255)
                                                                                                                                                 tcttcATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATAcc     <  2:645658/150‑1 (MQ=255)
                                                                                                                                                  cttcATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCt    >  2:970223/1‑150 (MQ=255)
                                                                                                                                                  cttcATAATCAGAACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCt    <  1:117255/150‑1 (MQ=255)
                                                                                                                                                    tcATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGa  >  1:869629/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACCTGA  >  NC_000913/3136483‑3136780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: