Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,140,352 G→C 6.3% R150G (CGC→GGC) ‡ hybE ← hydrogenase 2‑specific chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,140,3520GC 6.3% 411.9 / 14.6 126R150G (CGC→GGC) ‡hybEhydrogenase 2‑specific chaperone
Reads supporting (aligned to +/- strand):  ref base G (57/61);  new base C (3/5);  total (60/66)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.71e-01

Only 100 of 130 total aligned reads displayed.

ACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGT  >  NC_000913/3140203‑3140501
                                                                                                                                                     |                                                                                                                                                     
aCGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGcgcg                                                                                                                                                       <  2:745321/150‑1 (MQ=255)
  gCAGGAGAGCGCGCCAATATCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCAACTCCTGCGACCAAACAGCAGGGCGCGAc                                                                                                                                                     >  1:1091289/1‑150 (MQ=255)
     ggAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGcc                                                                                                                                                  <  2:1547776/150‑1 (MQ=255)
       agagCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCt                                                                                                                                                >  2:1229905/1‑150 (MQ=255)
        gagCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTg                                                                                                                                               <  1:1494092/150‑1 (MQ=255)
          gcgcgaCATTTTCCAGCCAGACGCAGGTGACGCGCTTAACATCGTGCTGAGCCGCCTGCAGTTTTAAAATTTCAACGGCTCTCAGGCAACGAGACAACTCATGCATTAACGCAACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCg                                                                                                                                             <  1:265502/150‑1 (MQ=255)
           cgcgcCAATTTCCAGCCACACGGCGGTGACGCGCTGAACATCGTGCTGCTCCGCCTGCCGTAGGATAATTTCAACGGCGATCTGGCAAAGAGACAACTCATGCATTTTCCACACTCCTGCGACCAAACAGCAGGGCTCGACGACCTGAgt                                                                                                                                            >  2:111840/1‑150 (MQ=255)
             cgcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCGGGGCGCGACGCCCTGCgtgt                                                                                                                                          >  2:1587700/1‑150 (MQ=255)
             cgcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGTCGCGCCCTGctgtt                                                                                                                                          <  1:355896/150‑5 (MQ=255)
               ccAATTTCCAGCCACACGGCGGTGACGCGCTTAACATAGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTCCGTGTgg                                                                                                                                        >  1:758691/1‑150 (MQ=255)
                cAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGTCGCGCCCTGctgtttgg                                                                                                                                       <  2:618845/150‑8 (MQ=255)
                  aTTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCGCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTAc                                                                                                                                     <  1:1090299/150‑1 (MQ=255)
                       cAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAACGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCACTGCGTGTGGTACATCCg                                                                                                                                >  2:220200/1‑150 (MQ=255)
                       cAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCg                                                                                                                                >  1:1069663/1‑150 (MQ=255)
                           cacaCGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGAtt                                                                                                                            >  1:1579658/1‑150 (MQ=255)
                           cacaCGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGAtt                                                                                                                            >  2:691083/1‑150 (MQ=255)
                           cacaCGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGAtt                                                                                                                            >  2:657077/1‑150 (MQ=255)
                           cacaCGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAAATTCAACGGCGCTCTCGCAAAGAGACAACTCATGCATTTTCGACACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGAtt                                                                                                                            >  1:1141008/1‑150 (MQ=255)
                            acacGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTc                                                                                                                           <  2:193262/150‑1 (MQ=255)
                               cggcggTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTg                                                                                                                        <  2:1634136/150‑1 (MQ=255)
                               cggcggTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTg                                                                                                                        <  2:1528354/150‑1 (MQ=255)
                                ggcggTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGAATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGa                                                                                                                       >  2:1733370/1‑150 (MQ=255)
                                     tGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGc                                                                                                                  >  1:363456/1‑150 (MQ=255)
                                      gACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGCCGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGcc                                                                                                                 >  1:124836/1‑149 (MQ=255)
                                          cgcTTAACGTCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACCGCAGGGCGCGCCGCCCTGCGTGTGGTACCTCCGGAGTCGTGACGGGCAGCGa                                                                                                             >  2:1143342/1‑150 (MQ=255)
                                               aaCATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTAGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGa                                                                                                        >  2:35172/1‑150 (MQ=255)
                                                aCATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGAt                                                                                                       >  2:304232/1‑150 (MQ=255)
                                                aCATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGAt                                                                                                       >  1:703772/1‑150 (MQ=255)
                                                 cATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACCGCAGGGCGCGACGCCCAGCGTGTGATACATCCGGATTCGTGACTGGCAGCGAAAGGATc                                                                                                      >  1:1050166/1‑150 (MQ=255)
                                                   tCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCATAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCATGTCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCAt                                                                                                    <  1:1046862/150‑1 (MQ=255)
                                                     gTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGCCTGGCAGCGAAAGGATCAGTc                                                                                                  >  2:1572103/1‑150 (MQ=255)
                                                      tgctgcTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAACCAGCAGGGCGCGACGCCCAGCGTGTCGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCg                                                                                                 >  2:294776/1‑150 (MQ=255)
                                                        ctgctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTg                                                                                               <  1:429715/150‑1 (MQ=255)
                                                        ctgctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTg                                                                                               >  1:709110/1‑150 (MQ=255)
                                                         tgctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTgc                                                                                              >  1:177193/1‑150 (MQ=255)
                                                         tgctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGAAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTgc                                                                                              <  1:358550/150‑1 (MQ=255)
                                                          gctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTgcg                                                                                             <  1:1549139/150‑1 (MQ=255)
                                                          gctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTgcg                                                                                             <  2:492961/150‑1 (MQ=255)
                                                          gctCCGCCTGCCGTGGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTgcg                                                                                             <  2:486475/150‑1 (MQ=255)
                                                            tCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCa                                                                                           >  1:1309166/1‑150 (MQ=255)
                                                                   gCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTAGCAGCGAAAGGATCATTCGTGCGCAGTCATcc                                                                                    >  1:20624/1‑150 (MQ=255)
                                                                     cGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGt                                                                                  <  2:266330/150‑1 (MQ=255)
                                                                      gTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCCGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTc                                                                                 <  1:1078064/150‑1 (MQ=255)
                                                                        tGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCCTGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAg                                                                               <  2:1262342/150‑1 (MQ=255)
                                                                        tGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAg                                                                               <  2:980034/150‑1 (MQ=255)
                                                                         ggATAATTTCAACGGCGCTCTGGCAAAGCGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGCTTCGTGACTGGCAGCGAAAGGATCAATCGTGCGCAGTCATCCGTCCgg                                                                              >  1:505830/1‑150 (MQ=255)
                                                                         ggATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAgg                                                                              <  2:1518484/150‑1 (MQ=255)
                                                                           aTAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACTTCCGGATTCGTGACTCGCAGCGAAAGGCTCATTCGTGCGCAGTCATCCGTCAGGCg                                                                            >  1:682541/1‑150 (MQ=255)
                                                                            tAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGCTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGt                                                                           >  1:105115/1‑150 (MQ=255)
                                                                             aaTTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGtt                                                                          >  2:36910/1‑150 (MQ=255)
                                                                              aTTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCa                                                                                <  2:372959/143‑1 (MQ=255)
                                                                              aTTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTg                                                                         >  2:1517969/1‑150 (MQ=255)
                                                                              aTTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTg                                                                         >  1:1654721/1‑150 (MQ=255)
                                                                                ttCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGc                                                                       <  2:1431537/150‑1 (MQ=255)
                                                                                tgctaCGGCGCGCTGGCAATGAGAGAAGTCATGCATTTTCGCATCTCCTGCGACCAATCTGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTGGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGc                                                                       <  2:739692/146‑1 (MQ=255)
                                                                                  cAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGccc                                                                     <  2:915308/150‑1 (MQ=255)
                                                                                   accGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCct                                                                    <  1:261084/148‑1 (MQ=255)
                                                                                     cGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCACCCGTCAGGCGTTGGCCctct                                                                  <  2:1456661/150‑1 (MQ=255)
                                                                                       gcgcTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTc                                                                <  1:394286/150‑1 (MQ=255)
                                                                                       gcgcTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTc                                                                <  2:1621153/150‑1 (MQ=255)
                                                                                          ctctGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAAt                                                             <  2:931588/150‑1 (MQ=255)
                                                                                           tctGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATc                                                            <  1:1229905/150‑1 (MQ=255)
                                                                                             tGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCgc                                                          >  1:1140070/1‑149 (MQ=255)
                                                                                              ggCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGAc                                                         >  2:900965/1‑150 (MQ=255)
                                                                                              ggCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTCCGAGTGGTAAATCCGGATTAGTGAATGGAAGAGAAAGGATCATTCGTACGAAGACATCCGTCAGGCGTTGGCCCTCTTCCATCGAc                                                         >  2:1547021/1‑150 (MQ=255)
                                                                                               gCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACa                                                        <  1:1636702/150‑1 (MQ=255)
                                                                                                cAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACAt                                                       >  1:1469020/1‑150 (MQ=255)
                                                                                                cAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACAt                                                       <  2:703772/150‑1 (MQ=255)
                                                                                                     agaCAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCACTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATAATTCGTGCGCAGTCATCCGTCAGGCTTTGGCCCGCATCAATCGACATGCggt                                                  >  2:1503361/1‑150 (MQ=255)
                                                                                                      gaCAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCtgtg                                                 >  2:173103/1‑150 (MQ=255)
                                                                                                      gaCAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCtgtg                                                 >  2:1070820/1‑150 (MQ=255)
                                                                                                        cAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCg                                               <  1:1358476/150‑1 (MQ=255)
                                                                                                        cAACTCATACATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCg                                               <  1:1330954/150‑1 (MQ=255)
                                                                                                            tCATGCATTTTCGCCTCTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAg                                           <  1:691083/150‑1 (MQ=255)
                                                                                                            tCATGCATTTTCGCCACTCCTGCGACCACACAGCAGGGCGCGACGCCCTGCGTGGGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAg                                           <  1:111840/150‑1 (MQ=255)
                                                                                                             cATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAgc                                          >  2:199954/1‑150 (MQ=255)
                                                                                                                gCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAgcggc                                       <  1:370978/150‑1 (MQ=255)
                                                                                                                gCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAgcggc                                       <  1:374033/150‑1 (MQ=255)
                                                                                                                 cATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAgcggcg                                      <  2:179483/150‑1 (MQ=255)
                                                                                                                 cATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAgcggcg                                      >  1:1524376/1‑150 (MQ=255)
                                                                                                                      tCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATc                                 <  1:593627/150‑1 (MQ=255)
                                                                                                                        gCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAg                               >  2:792180/1‑150 (MQ=255)
                                                                                                                          cACTCCTGCGACCAACCAGCTTGGCGCGACGGCCTGCGTGTGGTACATCCGGATGCGTGACTGGCTGCGAAAGGATCTTTCGTGCGCAGGCATCCGTCATGCGTTGGGCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCg                             <  2:1091289/150‑1 (MQ=255)
                                                                                                                           aCTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGa                            >  1:1534624/1‑150 (MQ=255)
                                                                                                                            cTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGaa                           >  2:377216/1‑150 (MQ=255)
                                                                                                                                tGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGCCATGCGGTGCGCAAGCGGCGACATCATCGAACAgg                       >  2:1153409/1‑150 (MQ=255)
                                                                                                                                  cGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGgag                     <  2:1473276/150‑1 (MQ=255)
                                                                                                                                  cGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGAAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGATAGCGGCGACATCAGCGAACAGgag                     >  2:956609/1‑150 (MQ=255)
                                                                                                                                   gACCAAACAGCTGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGgaga                    <  1:1587700/150‑1 (MQ=255)
                                                                                                                                   gACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGgaga                    <  1:182165/150‑1 (MQ=255)
                                                                                                                                   gACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGgaga                    <  2:207605/150‑1 (MQ=255)
                                                                                                                                     ccAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGgagaga                  >  1:843320/1‑150 (MQ=255)
                                                                                                                                      ccacacgcagGGCGTCGCGCCCTGCGTGTGGTACCTCCGGATTCGTGACTGGCAGCGATAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGat                 >  2:37379/7‑150 (MQ=255)
                                                                                                                                       cacacgcagGGCGTCGCGCCCTGCTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGGGACATCAGCGAACAGGAGAGatat               >  2:52897/6‑150 (MQ=255)
                                                                                                                                       aaaCAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGata                >  1:812969/1‑150 (MQ=255)
                                                                                                                                        acacgcagGGCGTCGCGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGatat               >  2:980582/5‑150 (MQ=255)
                                                                                                                                          aagcagGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTg             <  2:220565/149‑1 (MQ=255)
                                                                                                                                           agcagGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAATGGATCATTCGTTCGCAGTCATCCGTCAGGCTGTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGc            <  1:521416/150‑1 (MQ=255)
                                                                                                                                                     gACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGt  <  2:1678430/150‑1 (MQ=255)
                                                                                                                                                     gACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGt  <  1:259777/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGT  >  NC_000913/3140203‑3140501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: