Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,140,353 A→G 6.2% R149R (CGT→CGC hybE ← hydrogenase 2‑specific chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,140,3530AG 6.2% 369.1 / 11.7 129R149R (CGT→CGChybEhydrogenase 2‑specific chaperone
Reads supporting (aligned to +/- strand):  ref base A (55/64);  new base G (3/5);  total (60/69)
Fisher's exact test for biased strand distribution p-value = 7.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.00e-01

Only 100 of 129 total aligned reads displayed.

GCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGT  >  NC_000913/3140205‑3140501
                                                                                                                                                    |                                                                                                                                                    
gCAGGAGAGCGCGCCAATATCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCAACTCCTGCGACCAAACAGCAGGGCGCGAc                                                                                                                                                     >  1:1091289/1‑150 (MQ=255)
 cAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACg                                                                                                                                                    <  1:75563/150‑1 (MQ=255)
     agagCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCt                                                                                                                                                >  2:1229905/1‑150 (MQ=255)
      gagCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTg                                                                                                                                               <  1:1494092/150‑1 (MQ=255)
        gcgcgcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCg                                                                                                                                             <  2:1308461/150‑1 (MQ=255)
        gcgcgaCATTTTCCAGCCAGACGCAGGTGACGCGCTTAACATCGTGCTGAGCCGCCTGCAGTTTTAAAATTTCAACGGCTCTCAGGCAACGAGACAACTCATGCATTAACGCAACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCg                                                                                                                                             <  1:265502/150‑1 (MQ=255)
         cgcgcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGTCGCGCCCTGctg                                                                                                                                            <  1:874594/150‑3 (MQ=255)
         cgcgcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGTCGCGCCCTGctg                                                                                                                                            <  2:292320/150‑3 (MQ=255)
         cgcgcCAATTTCCAGCCACACGGCGGTGACGCGCTGAACATCGTGCTGCTCCGCCTGCCGTAGGATAATTTCAACGGCGATCTGGCAAAGAGACAACTCATGCATTTTCCACACTCCTGCGACCAAACAGCAGGGCTCGACGACCTGAgt                                                                                                                                            >  2:111840/1‑150 (MQ=255)
           cgcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCGGGGCGCGACGCCCTGCgtgt                                                                                                                                          >  2:1587700/1‑150 (MQ=255)
           cgcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGTCGCGCCCTGctgtt                                                                                                                                          <  1:355896/150‑5 (MQ=255)
             ccAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGTCGCGCCCTGctgtttg                                                                                                                                        <  2:207666/150‑7 (MQ=255)
              cAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGTCGCGCCCTGctgtttgg                                                                                                                                       <  2:618845/150‑8 (MQ=255)
                aTTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTAc                                                                                                                                     >  2:1636702/1‑150 (MQ=255)
                aTTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCGCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTAc                                                                                                                                     <  1:1090299/150‑1 (MQ=255)
                    ccAGCCACCCGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATcc                                                                                                                                 <  1:1497959/150‑1 (MQ=255)
                     cAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAACGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCACTGCGTGTGGTACATCCg                                                                                                                                >  2:220200/1‑150 (MQ=255)
                     cAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCg                                                                                                                                >  1:1069663/1‑150 (MQ=255)
                       gCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAAAAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGa                                                                                                                              >  1:220565/1‑150 (MQ=255)
                         cacaCGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGAtt                                                                                                                            >  2:657077/1‑150 (MQ=255)
                         cacaCGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAAATTCAACGGCGCTCTCGCAAAGAGACAACTCATGCATTTTCGACACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGAtt                                                                                                                            >  1:1141008/1‑150 (MQ=255)
                          acacGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTc                                                                                                                           <  2:193262/150‑1 (MQ=255)
                             cggcggTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTg                                                                                                                        <  2:1528354/150‑1 (MQ=255)
                             cggcggTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTg                                                                                                                        <  2:1634136/150‑1 (MQ=255)
                              ggcggTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGAATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGa                                                                                                                       >  2:1733370/1‑150 (MQ=255)
                                   tGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGc                                                                                                                  >  1:363456/1‑150 (MQ=255)
                                    gACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGCCGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGcc                                                                                                                 >  1:124836/1‑149 (MQ=255)
                                      cgcgCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGc                                                                                                               <  2:588371/150‑1 (MQ=255)
                                        cgcTTAACGTCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACCGCAGGGCGCGCCGCCCTGCGTGTGGTACCTCCGGAGTCGTGACGGGCAGCGa                                                                                                             >  2:1143342/1‑150 (MQ=255)
                                              aCATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGAt                                                                                                       >  2:304232/1‑150 (MQ=255)
                                              aCATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGAt                                                                                                       >  1:703772/1‑150 (MQ=255)
                                               cATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACCGCAGGGCGCGACGCCCAGCGTGTGATACATCCGGATTCGTGACTGGCAGCGAAAGGATc                                                                                                      >  1:1050166/1‑150 (MQ=255)
                                                 tCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCATAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCATGTCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCAt                                                                                                    <  1:1046862/150‑1 (MQ=255)
                                                   gTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGCCTGGCAGCGAAAGGATCAGTc                                                                                                  >  2:1572103/1‑150 (MQ=255)
                                                    tgctgcTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAACCAGCAGGGCGCGACGCCCAGCGTGTCGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCg                                                                                                 >  2:294776/1‑150 (MQ=255)
                                                      ctgctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTg                                                                                               <  1:429715/150‑1 (MQ=255)
                                                      ctgctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTg                                                                                               >  1:709110/1‑150 (MQ=255)
                                                        gctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTgcg                                                                                             <  2:492961/150‑1 (MQ=255)
                                                        gctCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTgcg                                                                                             <  1:1549139/150‑1 (MQ=255)
                                                        gctCCGCCTGCCGTTGGATAATTTCAACCGCGCTCTGGCACAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTgcg                                                                                             <  1:482672/150‑1 (MQ=255)
                                                        gctCCGCCTGCCGTGGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTgcg                                                                                             <  2:486475/150‑1 (MQ=255)
                                                          tCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCa                                                                                           >  1:1309166/1‑150 (MQ=255)
                                                             gCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTc                                                                                        <  1:298681/150‑1 (MQ=255)
                                                                 gCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTAGCAGCGAAAGGATCATTCGTGCGCAGTCATcc                                                                                    >  1:20624/1‑150 (MQ=255)
                                                                   cggtGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCCTGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGt                                                                                  <  1:63359/147‑1 (MQ=255)
                                                                   cGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGt                                                                                  <  2:266330/150‑1 (MQ=255)
                                                                    gTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCCGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTc                                                                                 <  1:1078064/150‑1 (MQ=255)
                                                                      tGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCCTGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAg                                                                               <  2:1262342/150‑1 (MQ=255)
                                                                      tGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAg                                                                               <  2:980034/150‑1 (MQ=255)
                                                                       ggATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCCgg                                                                              >  2:42054/1‑150 (MQ=255)
                                                                       ggATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAgg                                                                              <  2:1518484/150‑1 (MQ=255)
                                                                         aTAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACTTCCGGATTCGTGACTCGCAGCGAAAGGCTCATTCGTGCGCAGTCATCCGTCAGGCg                                                                            >  1:682541/1‑150 (MQ=255)
                                                                          tAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGCTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGt                                                                           >  1:105115/1‑150 (MQ=255)
                                                                           aaTTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGtt                                                                          >  2:36910/1‑150 (MQ=255)
                                                                            aTTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCa                                                                                >  1:372959/1‑143 (MQ=255)
                                                                            aTTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCa                                                                                <  2:372959/143‑1 (MQ=255)
                                                                            aTTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTg                                                                         >  1:1654721/1‑150 (MQ=255)
                                                                              ttCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGc                                                                       <  2:1431537/150‑1 (MQ=255)
                                                                              ttCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGc                                                                       <  2:700058/150‑1 (MQ=255)
                                                                              tgctaCGGCGCGCTGGCAATGAGAGAAGTCATGCATTTTCGCATCTCCTGCGACCAATCTGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTGGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGc                                                                       <  2:739692/146‑1 (MQ=255)
                                                                                cAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGccc                                                                     <  2:915308/150‑1 (MQ=255)
                                                                                cAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGccc                                                                     <  1:1716555/150‑1 (MQ=255)
                                                                                 accGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCct                                                                    <  1:261084/148‑1 (MQ=255)
                                                                                 aaCGGCGCTCTGGCAAAGAGAAAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCct                                                                    >  1:1146831/1‑150 (MQ=255)
                                                                                    ggCGCGCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCtt                                                                 <  2:1486042/150‑1 (MQ=255)
                                                                                     gcgcTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTc                                                                <  1:394286/150‑1 (MQ=255)
                                                                                        ctctGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAAt                                                             <  2:931588/150‑1 (MQ=255)
                                                                                           tGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCgc                                                          >  1:1140070/1‑149 (MQ=255)
                                                                                            ggCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGAc                                                         >  2:900965/1‑150 (MQ=255)
                                                                                            ggCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTCCGAGTGGTAAATCCGGATTAGTGAATGGAAGAGAAAGGATCATTCGTACGAAGACATCCGTCAGGCGTTGGCCCTCTTCCATCGAc                                                         >  2:1547021/1‑150 (MQ=255)
                                                                                             gCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACa                                                        <  1:1636702/150‑1 (MQ=255)
                                                                                              cAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACAt                                                       >  1:1469020/1‑150 (MQ=255)
                                                                                              cAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACAt                                                       <  2:703772/150‑1 (MQ=255)
                                                                                                   agaCAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCACTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATAATTCGTGCGCAGTCATCCGTCAGGCTTTGGCCCGCATCAATCGACATGCggt                                                  >  2:1503361/1‑150 (MQ=255)
                                                                                                    gaCAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCtgtg                                                 >  2:1070820/1‑150 (MQ=255)
                                                                                                      cAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCg                                               <  1:1358476/150‑1 (MQ=255)
                                                                                                      cAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCg                                               <  2:1519001/150‑1 (MQ=255)
                                                                                                      cAACTCATACATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCg                                               <  1:1330954/150‑1 (MQ=255)
                                                                                                          tCATGCATTTTCGCCACTCCTGCGACCACACAGCAGGGCGCGACGCCCTGCGTGGGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAg                                           <  1:111840/150‑1 (MQ=255)
                                                                                                              gCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAgcggc                                       <  1:370978/150‑1 (MQ=255)
                                                                                                               cATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAgcggcg                                      <  2:363456/150‑1 (MQ=255)
                                                                                                               cATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAgcggcg                                      <  2:179483/150‑1 (MQ=255)
                                                                                                                    tCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATc                                 <  1:593627/150‑1 (MQ=255)
                                                                                                                      gCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAg                               >  2:792180/1‑150 (MQ=255)
                                                                                                                         aCTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGa                            >  1:1534624/1‑150 (MQ=255)
                                                                                                                          cTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGaa                           >  2:377216/1‑150 (MQ=255)
                                                                                                                            ccTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACa                         >  1:1549387/1‑150 (MQ=255)
                                                                                                                                cGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGgag                     <  2:1473276/150‑1 (MQ=255)
                                                                                                                                cGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGAAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGATAGCGGCGACATCAGCGAACAGgag                     >  2:956609/1‑150 (MQ=255)
                                                                                                                                 gACCAAACAGCTGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGgaga                    <  1:1587700/150‑1 (MQ=255)
                                                                                                                                 gACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGgaga                    <  1:182165/150‑1 (MQ=255)
                                                                                                                                 gACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGgaga                    <  2:207605/150‑1 (MQ=255)
                                                                                                                                   ccAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGgagaga                  >  1:843320/1‑150 (MQ=255)
                                                                                                                                     aaaCAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGata                >  1:812969/1‑150 (MQ=255)
                                                                                                                                      acacgcagGGCGTCGCGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGatat               >  2:980582/5‑150 (MQ=255)
                                                                                                                                         agcagGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAATGGATCATTCGTTCGCAGTCATCCGTCAGGCTGTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGc            <  1:521416/150‑1 (MQ=255)
                                                                                                                                          gcagGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGAGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCCTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGcc           >  2:1132611/1‑149 (MQ=255)
                                                                                                                                            agGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAACGAACAGGAGAGATATTGCGaa         <  2:177193/150‑1 (MQ=255)
                                                                                                                                                   gACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGt  <  2:1678430/150‑1 (MQ=255)
                                                                                                                                                   gACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGt  <  2:1579658/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGGCGTTGGCCCTCTTCAATCGACATGCTGTGCGAAAGCGGCGACATCAGCGAACAGGAGAGATATTGCGAAACACCGT  >  NC_000913/3140205‑3140501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: