Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,140,859 T→G 5.8% E143A (GAG→GCG)  hybD ← maturation protease for hydrogenase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,140,8590TG 5.8% 342.3 / 8.8 121E143A (GAG→GCG) hybDmaturation protease for hydrogenase 2
Reads supporting (aligned to +/- strand):  ref base T (55/58);  new base G (3/4);  total (59/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.79e-01

Only 100 of 124 total aligned reads displayed.

ATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTG  >  NC_000913/3140710‑3141007
                                                                                                                                                     |                                                                                                                                                    
agcGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTAGCGCCTGCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCt                                                                                                                                                      <  2:67739/148‑1 (MQ=255)
aTCGTGCATCGAACGCCGGGCAATTTCTTCAAACTCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCt                                                                                                                                                      <  1:835951/150‑1 (MQ=255)
aTCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCt                                                                                                                                                      <  1:1280403/150‑1 (MQ=255)
  cGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTTAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCa                                                                                                                                                    <  2:1433101/150‑1 (MQ=255)
    tGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAg                                                                                                                                                  >  2:893815/1‑150 (MQ=255)
      cATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAgcg                                                                                                                                                <  2:1674767/150‑1 (MQ=255)
         cGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAg                                                                                                                                             <  1:218009/150‑1 (MQ=255)
         cGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAg                                                                                                                                             >  1:815349/1‑150 (MQ=255)
             cGCCGGGCAATTTCCTAAAACGCTGCCTGTACTTGCGCGTGCGGGGAGGGCTGGAACCCTGCTATCTCTTCAGTACTGAATCGCCTCCAGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCGCTTGAGCAGGTTc                                                                                                                                         <  1:1071613/150‑1 (MQ=255)
                 gggCAATTTCTTCAAACGATGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCGCTTGAGCAGGTTctggc                                                                                                                                     <  1:1401529/150‑5 (MQ=255)
                  ggCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCGCTTGAGCAGGTTctggct                                                                                                                                    <  1:613350/150‑6 (MQ=255)
                      aTTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGc                                                                                                                                >  1:32845/1‑150 (MQ=255)
                      aTTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGc                                                                                                                                >  1:200402/1‑150 (MQ=255)
                       tttCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGTATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCt                                                                                                                               <  1:1194654/150‑1 (MQ=255)
                          cttcAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCa                                                                                                                            >  2:1304189/1‑150 (MQ=255)
                          cttcAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCa                                                                                                                            >  2:380725/1‑150 (MQ=255)
                          cttcAAACGCGGCCGGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCa                                                                                                                            <  2:1103200/150‑1 (MQ=255)
                          cttcAAAAGATGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCAAGAATCGCCTCCCGTGGGATGGCTTCAACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCa                                                                                                                            >  2:1304196/1‑150 (MQ=255)
                            tcAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCACGCGCAGGTTCAATCATTGCTTCAAc                                                                                                                          >  2:352178/1‑150 (MQ=255)
                              aaaCGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCg                                                                                                                        >  2:1215145/1‑150 (MQ=255)
                               aaCGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGt                                                                                                                       <  1:1734084/150‑1 (MQ=255)
                                  gCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCgg                                                                                                                    <  1:814318/150‑1 (MQ=255)
                                     gCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGt                                                                                                                 >  1:1217679/1‑150 (MQ=255)
                                         ttACTTTCGCCTTCGGGGAGGTCTTGAAACCTGCTATCTCTTCAGTCAAGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGTCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAg                                                                                                             <  2:1071197/149‑1 (MQ=255)
                                          tACTTGAGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCTCCTCCCGTGGGATGGCTTCCACGCCAGATTCAAGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCTATCGTTGCTTCAACCGTCGGCGTTAAGc                                                                                                            <  1:1547021/150‑1 (MQ=255)
                                           aCTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGcc                                                                                                           <  2:1656867/150‑1 (MQ=255)
                                           aCTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGcc                                                                                                           <  2:777953/150‑1 (MQ=255)
                                             ttGCGCCTGCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGa                                                                                                         <  1:1118040/150‑1 (MQ=255)
                                               gcgcCTTCGGGGAGGTCTGGAAACCTGCTAGCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGAtg                                                                                                       <  1:1047582/150‑1 (MQ=255)
                                                 gcCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGAtgtg                                                                                                     >  2:1674132/1‑150 (MQ=255)
                                                       ggggAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTc                                                                                               >  2:166180/1‑150 (MQ=255)
                                                       ggggAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTc                                                                                               >  1:1364343/1‑150 (MQ=255)
                                                           aGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGc                                                                                           <  1:423352/150‑1 (MQ=255)
                                                              gcTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGAt                                                                                        <  2:1392322/149‑1 (MQ=255)
                                                               cTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGAtt                                                                                       >  2:189403/1‑150 (MQ=255)
                                                               cTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAAAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGAtt                                                                                       >  1:342960/1‑150 (MQ=255)
                                                                   aaaCCTGCTATCTCTTCAGTAATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGACCCTGCTCAAGCGCAGGTTCAATCTTTGCTGCTACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCgg                                                                                   <  2:1041707/150‑1 (MQ=255)
                                                                      ccTGCTATCTCTTCAGTCATGAATCGCCTGCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGAt                                                                                <  1:107118/150‑1 (MQ=255)
                                                                       cTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATc                                                                               >  1:389431/1‑150 (MQ=255)
                                                                         gCTATCTCTTCAGTCATGAATCGCCTCCATAGTGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCCGAACCTGCAAAAGCGCACGTACAATCATTGCTTCAACCGTCGGCTTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATAAc                                                                             >  2:1131683/1‑150 (MQ=255)
                                                                              ctctTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGa                                                                        >  2:215716/1‑150 (MQ=255)
                                                                               tctTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGAc                                                                       >  1:994114/1‑150 (MQ=255)
                                                                                ctTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCGACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGAcc                                                                      >  1:1068631/1‑150 (MQ=255)
                                                                                ctTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGccc                                                                      >  2:457855/1‑150 (MQ=255)
                                                                                ctTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGAcc                                                                      >  1:445166/1‑150 (MQ=255)
                                                                                  tCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAg                                                                    <  1:1358869/150‑1 (MQ=255)
                                                                                  tCAGTCATGAATCGCCACCCGTCGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCACGCGCAGGTTCAATCATTGCATCACCCGGCGGCGCTAAGCCGATGTGTGGCTACAGCGATTCCGGGATCACGCCGACCAg                                                                    <  1:841392/150‑1 (MQ=255)
                                                                                    aGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAggg                                                                  <  1:1674132/150‑1 (MQ=255)
                                                                                          taaTCGCCTCCCGTGGGATGGCTGCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGTCGACCAGGGTCAGCt                                                            <  1:1085288/149‑1 (MQ=255)
                                                                                          gAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCt                                                            <  1:898921/150‑1 (MQ=255)
                                                                                          caaTCGCCTCCCGGGGGATGGCGTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCt                                                            <  1:76827/149‑1 (MQ=255)
                                                                                           aaTCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCtt                                                           >  2:1343268/1‑150 (MQ=255)
                                                                                           aaTCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCtt                                                           >  2:1583669/1‑150 (MQ=255)
                                                                                                  tCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGa                                                    <  2:1246604/150‑1 (MQ=255)
                                                                                                   cccGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGaa                                                   >  2:1275094/1‑150 (MQ=255)
                                                                                                   cccGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGaa                                                   >  1:1718535/1‑150 (MQ=255)
                                                                                                    ccGTGGGATGGGTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGaaa                                                  <  2:1123145/150‑1 (MQ=255)
                                                                                                      gTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACt                                                <  1:1467883/150‑1 (MQ=255)
                                                                                                      gTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACt                                                <  2:843734/150‑1 (MQ=255)
                                                                                                       tGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGTGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACta                                               >  2:53781/1‑149 (MQ=255)
                                                                                                       ggggATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTc                                               <  2:165470/149‑1 (MQ=255)
                                                                                                        gggATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCg                                              >  1:1541469/1‑150 (MQ=255)
                                                                                                          gATGGCTTCCACGCCAGATTCACGCAGCTCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGcc                                            <  1:1343268/150‑1 (MQ=255)
                                                                                                          gATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGcc                                            <  1:1583669/150‑1 (MQ=255)
                                                                                                            tGGCTGCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGAACAGGGTCAGCTTTTTCGGAAACTCGCCgg                                          <  1:420603/150‑1 (MQ=255)
                                                                                                             ggCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGt                                         <  1:179694/150‑1 (MQ=255)
                                                                                                             ggCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGGTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGt                                         <  2:193826/150‑1 (MQ=255)
                                                                                                              gCTTCCACGCCTGATTCACGCAGCGCAGCCAGAACGTGCTCAAGCGCAGGTTCAATCATTGCTTCAAACGTCGGCGTTAAGCCGATGGGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTg                                        <  1:1161387/150‑1 (MQ=255)
                                                                                                              gCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGACGGTg                                        >  1:864072/1‑150 (MQ=255)
                                                                                                               cTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCACCCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGCTTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTgc                                       >  1:1321623/1‑149 (MQ=255)
                                                                                                               cTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGa                                       >  2:1444891/1‑150 (MQ=255)
                                                                                                               cTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGa                                       >  2:1591823/1‑150 (MQ=255)
                                                                                                               cTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGa                                       >  1:50500/1‑150 (MQ=255)
                                                                                                               cTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGa                                       <  2:1217679/150‑1 (MQ=255)
                                                                                                                 tCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAg                                     <  1:1242435/150‑1 (MQ=255)
                                                                                                                 tCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATACCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAg                                     >  1:1553228/1‑150 (MQ=255)
                                                                                                                      gCCAGATTCACGCAGCGCAGCCAGACCCTGCTCACGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGGGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAg                                <  2:334767/150‑1 (MQ=255)
                                                                                                                         aGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGc                             <  1:452962/150‑1 (MQ=255)
                                                                                                                               aCGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACaa                       <  1:1242285/150‑1 (MQ=255)
                                                                                                                               aCGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACaa                       >  2:68256/1‑150 (MQ=255)
                                                                                                                                 gcagcgcagcCAGAACCTGCTGATGCGCAGGTTCAATCATTGCTTCAACCGTCAGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGa                     <  1:1601898/150‑1 (MQ=255)
                                                                                                                                   agcgcagcCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATAACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACg                   <  2:1559793/150‑1 (MQ=255)
                                                                                                                                       cagcCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCgg               >  2:472797/1‑150 (MQ=255)
                                                                                                                                         gcCAGAACCTGCTCAAGCGCAGGTTCAATTATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggcc             <  2:100556/150‑1 (MQ=255)
                                                                                                                                         gcCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGCCCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggcc             >  2:64574/1‑150 (MQ=255)
                                                                                                                                         gcCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggcc             >  1:80677/1‑150 (MQ=255)
                                                                                                                                         gattgAACCTGCGCTTGAGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggcc             >  1:503913/5‑150 (MQ=255)
                                                                                                                                         gattgAACCTGCGCTTGAGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggcc             >  1:1290232/5‑150 (MQ=255)
                                                                                                                                           cAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggccag           >  1:1240253/1‑150 (MQ=255)
                                                                                                                                           cAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggccag           <  2:431885/150‑1 (MQ=255)
                                                                                                                                            aGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGATACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggccagg          >  2:697340/1‑150 (MQ=255)
                                                                                                                                              acccTGCTCACGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCGCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAACCTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggccaggcc        <  1:1695819/148‑1 (MQ=255)
                                                                                                                                                  tGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCCGGGCCGACACGACGTCGGCCAGGCCAAGc    >  2:1566778/1‑150 (MQ=255)
                                                                                                                                                  tGCTCAAGCGCAGGTTCAATCATGGCTTCACCCGGCGGCGTGAAGCCGATGGGTGGCTCCAGCGATGCCGGGATCACGCCGACCAGGGGCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGc    <  1:1290006/150‑1 (MQ=255)
                                                                                                                                                   gCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACCCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCt   <  1:380725/150‑1 (MQ=255)
                                                                                                                                                   gCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGGCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCt   <  1:680227/150‑1 (MQ=255)
                                                                                                                                                    cTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTg  <  2:32845/150‑1 (MQ=255)
                                                                                                                                                    cTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTg  >  2:779131/1‑150 (MQ=255)
                                                                                                                                                    cTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTg  <  2:200402/150‑1 (MQ=255)
                                                                                                                                                    cTCAAGCGCAGGTTCAATCATTGCTTCAACCGGCGGCGTTAAGCCGATGTGGGGCTCCAGCGATTCCGGGAGCACGCCGACCAGGGTCAGCTTTATCGGACACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTg  <  1:1153314/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTG  >  NC_000913/3140710‑3141007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: