Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,140,864 C→A 5.4% A141A (GCG→GCT hybD ← maturation protease for hydrogenase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,140,8640CA 5.4% 368.6 / 8.1 119A141A (GCG→GCThybDmaturation protease for hydrogenase 2
Reads supporting (aligned to +/- strand):  ref base C (55/57);  new base A (3/4);  total (58/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01

Only 100 of 122 total aligned reads displayed.

CATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGG  >  NC_000913/3140716‑3141013
                                                                                                                                                    |                                                                                                                                                     
cATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAgcg                                                                                                                                                      <  2:1674767/150‑1 (MQ=255)
   cGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAg                                                                                                                                                   <  1:218009/150‑1 (MQ=255)
       cGCCGGGCAATTTCCTAAAACGCTGCCTGTACTTGCGCGTGCGGGGAGGGCTGGAACCCTGCTATCTCTTCAGTACTGAATCGCCTCCAGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCGCTTGAGCAGGTTc                                                                                                                                               <  1:1071613/150‑1 (MQ=255)
         ccGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCaa                                                                                                                                             >  2:638833/1‑150 (MQ=255)
           gggCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCGCTTGAGCAGGTTctggc                                                                                                                                           <  1:1559161/150‑5 (MQ=255)
           gggCAATTTCTTCAAACGATGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCGCTTGAGCAGGTTctggc                                                                                                                                           <  1:1401529/150‑5 (MQ=255)
            ggCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCa                                                                                                                                          >  2:1242435/1‑150 (MQ=255)
            ggCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCGCTTGAGCAGGTTctggct                                                                                                                                          <  1:613350/150‑6 (MQ=255)
                aTTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGc                                                                                                                                      >  1:32845/1‑150 (MQ=255)
                aTTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGc                                                                                                                                      >  1:200402/1‑150 (MQ=255)
                 tttCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGTATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCt                                                                                                                                     <  1:1194654/150‑1 (MQ=255)
                    cttcAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCa                                                                                                                                  >  2:380725/1‑150 (MQ=255)
                    cttcAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCa                                                                                                                                  >  2:1304189/1‑150 (MQ=255)
                    cttcAAACGCGGCCGGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCa                                                                                                                                  <  2:1103200/150‑1 (MQ=255)
                    cttcAAAAGATGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCAAGAATCGCCTCCCGTGGGATGGCTTCAACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCa                                                                                                                                  >  2:1304196/1‑150 (MQ=255)
                        aaaCGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCg                                                                                                                              >  2:1215145/1‑150 (MQ=255)
                         aaCGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGt                                                                                                                             <  1:1734084/150‑1 (MQ=255)
                            gCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCgg                                                                                                                          <  1:814318/150‑1 (MQ=255)
                               gCCTGAACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGt                                                                                                                       >  1:1725032/1‑150 (MQ=255)
                                ccggTACTTGCGCCTTCGGGGAGGTCTGGAAACATGCTATCTCTTCAGTCATGAATCTCCTCCCGGGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGtt                                                                                                                      <  2:308294/147‑1 (MQ=255)
                                   ttACTTTCGCCTTCGGGGAGGTCTTGAAACCTGCTATCTCTTCAGTCAAGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGTCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAg                                                                                                                   <  2:1071197/149‑1 (MQ=255)
                                     aCTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGcc                                                                                                                 <  2:1656867/150‑1 (MQ=255)
                                     aCTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGcc                                                                                                                 <  2:777953/150‑1 (MQ=255)
                                       ttGCGCCTGCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGa                                                                                                               <  1:1118040/150‑1 (MQ=255)
                                         gcgcCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGAtg                                                                                                             >  2:1342140/1‑150 (MQ=255)
                                         gcgcCTTCGGGGAGGTCTGGAAACCTGCTAGCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGAtg                                                                                                             <  1:1047582/150‑1 (MQ=255)
                                            ccTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGAtgtgt                                                                                                          >  1:1410753/1‑150 (MQ=255)
                                             cTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGAtgtgtg                                                                                                         >  1:835173/1‑150 (MQ=255)
                                                 ggggAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTc                                                                                                     >  2:166180/1‑150 (MQ=255)
                                                     aGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGc                                                                                                 <  1:423352/150‑1 (MQ=255)
                                                         cTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGAtt                                                                                             >  2:189403/1‑150 (MQ=255)
                                                         cTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAAAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGAtt                                                                                             >  1:342960/1‑150 (MQ=255)
                                                             aaaCCTGCTATCTCTTCAGTAATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGACCCTGCTCAAGCGCAGGTTCAATCTTTGCTGCTACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCgg                                                                                         <  2:1041707/150‑1 (MQ=255)
                                                                ccTGCTATCTCTTCAGTCATGAATCGCCTGCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGAt                                                                                      <  1:107118/150‑1 (MQ=255)
                                                                 cTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATc                                                                                     >  1:389431/1‑150 (MQ=255)
                                                                   gCTATCTCTTCAGTCATGAATCGCCTCCATAGTGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCCGAACCTGCAAAAGCGCACGTACAATCATTGCTTCAACCGTCGGCTTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATAAc                                                                                   >  2:1131683/1‑150 (MQ=255)
                                                                        ctctTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGa                                                                              >  2:215716/1‑150 (MQ=255)
                                                                         tctTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGAc                                                                             >  1:994114/1‑150 (MQ=255)
                                                                          ctTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCGACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGAcc                                                                            >  1:1068631/1‑150 (MQ=255)
                                                                          ctTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGccc                                                                            >  2:457855/1‑150 (MQ=255)
                                                                            tCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAg                                                                          <  1:1358869/150‑1 (MQ=255)
                                                                            tCAGTCATGAATCGCCACCCGTCGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCACGCGCAGGTTCAATCATTGCATCACCCGGCGGCGCTAAGCCGATGTGTGGCTACAGCGATTCCGGGATCACGCCGACCAg                                                                          <  1:841392/150‑1 (MQ=255)
                                                                                 cATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCCCGCAGCGCAGCCAGAACCTCCTCAAGCGCAGGTACAATCATTGCTTCCACCGTCGGCGTTAAGCCCATGAGTGGCTCCAGCGATTCCGGGATCACGCCGACCAggggcc                                                                     >  1:1336579/1‑147 (MQ=255)
                                                                                    taaTCGCCTCCCGTGGGATGGCTGCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGTCGACCAGGGTCAGCt                                                                  <  1:1085288/149‑1 (MQ=255)
                                                                                    gAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCt                                                                  <  1:898921/150‑1 (MQ=255)
                                                                                    caaTCGCCTCCCGGGGGATGGCGTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCt                                                                  <  1:76827/149‑1 (MQ=255)
                                                                                     aaTCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCtt                                                                 >  2:1343268/1‑150 (MQ=255)
                                                                                         gCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGcc                                                                                >  1:419376/1‑131 (MQ=255)
                                                                                         gCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGcc                                                                                <  2:419376/131‑1 (MQ=255)
                                                                                          ccTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCg                                                            <  1:1207558/150‑1 (MQ=255)
                                                                                             cccGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAGGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGaa                                                         >  2:1116637/1‑150 (MQ=255)
                                                                                             cccGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGaa                                                         <  2:162435/150‑1 (MQ=255)
                                                                                             cccGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGaa                                                         >  1:1718535/1‑150 (MQ=255)
                                                                                             cccGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGaa                                                         >  2:1275094/1‑150 (MQ=255)
                                                                                                gTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACt                                                      <  2:843734/150‑1 (MQ=255)
                                                                                                gTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACt                                                      <  1:1467883/150‑1 (MQ=255)
                                                                                                 tGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGTGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACta                                                     >  2:53781/1‑149 (MQ=255)
                                                                                                 ggggATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTc                                                     <  2:165470/149‑1 (MQ=255)
                                                                                                  gggATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCg                                                    >  1:1541469/1‑150 (MQ=255)
                                                                                                  gggATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCg                                                    >  1:317459/1‑150 (MQ=255)
                                                                                                    gATGGCTTCCACGCCAGATTCACGCAGCTCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGcc                                                  <  1:1343268/150‑1 (MQ=255)
                                                                                                    gATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGcc                                                  <  1:1583669/150‑1 (MQ=255)
                                                                                                      tGGCTGCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGAACAGGGTCAGCTTTTTCGGAAACTCGCCgg                                                <  1:420603/150‑1 (MQ=255)
                                                                                                        gCTTCCACGCCTGATTCACGCAGCGCAGCCAGAACGTGCTCAAGCGCAGGTTCAATCATTGCTTCAAACGTCGGCGTTAAGCCGATGGGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTg                                              <  1:1161387/150‑1 (MQ=255)
                                                                                                        gCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGACGGTg                                              >  1:864072/1‑150 (MQ=255)
                                                                                                         cTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCACCCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGCTTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTgc                                             >  1:1321623/1‑149 (MQ=255)
                                                                                                         cTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGa                                             <  2:1217679/150‑1 (MQ=255)
                                                                                                         cTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGa                                             >  2:1444891/1‑150 (MQ=255)
                                                                                                         cTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGa                                             >  2:1591823/1‑150 (MQ=255)
                                                                                                         cTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGa                                             >  1:50500/1‑150 (MQ=255)
                                                                                                           tCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAg                                           <  1:1242435/150‑1 (MQ=255)
                                                                                                           tCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATACCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAg                                           >  1:1553228/1‑150 (MQ=255)
                                                                                                                gCCAGATTCACGCAGCGCAGCCAGACCCTGCTCACGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGGGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAg                                      <  2:334767/150‑1 (MQ=255)
                                                                                                                   aGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGc                                   <  1:452962/150‑1 (MQ=255)
                                                                                                                         aCGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACaa                             >  2:68256/1‑150 (MQ=255)
                                                                                                                         aCGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACaa                             <  1:1242285/150‑1 (MQ=255)
                                                                                                                          cgcagcgcagCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGATGCGCCGGGCCGACAAg                            >  1:467097/1‑150 (MQ=255)
                                                                                                                           gcagcgcagcCAGAACCTGCTGATGCGCAGGTTCAATCATTGCTTCAACCGTCAGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGa                           <  1:1601898/150‑1 (MQ=255)
                                                                                                                             agcgcagcCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATAACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACg                         <  2:1559793/150‑1 (MQ=255)
                                                                                                                                   gcCAGAACCTGCTCAAGCGCAGGTTCAATTATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggcc                   <  2:100556/150‑1 (MQ=255)
                                                                                                                                   gcCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGCCCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggcc                   >  2:64574/1‑150 (MQ=255)
                                                                                                                                   gcCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggcc                   >  1:80677/1‑150 (MQ=255)
                                                                                                                                   gattgAACCTGCGCTTGAGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggcc                   >  1:1290232/5‑150 (MQ=255)
                                                                                                                                   gattgAACCTGCGCTTGAGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggcc                   >  2:1462757/5‑150 (MQ=255)
                                                                                                                                   gattgAACCTGCGCTTGAGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggcc                   >  1:503913/5‑150 (MQ=255)
                                                                                                                                    ccAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggcca                  <  1:1275094/150‑1 (MQ=255)
                                                                                                                                     cAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggccag                 >  1:1240253/1‑150 (MQ=255)
                                                                                                                                     cAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggccag                 <  2:431885/150‑1 (MQ=255)
                                                                                                                                      aGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGATACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggccagg                >  2:697340/1‑150 (MQ=255)
                                                                                                                                        acccTGCTCACGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCGCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAACCTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggccaggcc              <  1:1695819/148‑1 (MQ=255)
                                                                                                                                          ccTGCTCAAGCGCAAGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCaa            >  1:733232/1‑150 (MQ=255)
                                                                                                                                            tGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCCGGGCCGACACGACGTCGGCCAGGCCAAGc          >  2:1566778/1‑150 (MQ=255)
                                                                                                                                            tGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGA‑‑TGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTg        <  2:1728257/150‑1 (MQ=255)
                                                                                                                                              cTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTg        <  2:32845/150‑1 (MQ=255)
                                                                                                                                              cTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTg        <  2:200402/150‑1 (MQ=255)
                                                                                                                                              cTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTg        >  2:779131/1‑150 (MQ=255)
                                                                                                                                              cTCAAGCGCAGGTTCAATCATTGCTTCAACCGGCGGCGTTAAGCCGATGTGGGGCTCCAGCGATTCCGGGAGCACGCCGACCAGGGTCAGCTTTATCGGACACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTg        <  1:1153314/150‑1 (MQ=255)
                                                                                                                                                cAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGAt      <  2:1725032/150‑1 (MQ=255)
                                                                                                                                                    cgcAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGGCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCgg  <  2:835173/150‑1 (MQ=255)
                                                                                                                                                    cgcAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATAGCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGAGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCgg  >  1:163688/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGCTGATGCGG  >  NC_000913/3140716‑3141013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: