Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,146,761 T→G 7.6% intergenic (‑24/+95) yghW ← / ← yghX DUF2623 family protein/pseudogene, dienlactone hydrolase family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,146,7610TG 7.6% 352.9 / 15.6 128intergenic (‑24/+95)yghW/yghXDUF2623 family protein/pseudogene, dienlactone hydrolase family
Reads supporting (aligned to +/- strand):  ref base T (48/70);  new base G (6/4);  total (54/74)
Fisher's exact test for biased strand distribution p-value = 3.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.55e-01

Only 100 of 132 total aligned reads displayed.

CCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATCGGC  >  NC_000913/3146612‑3146909
                                                                                                                                                     |                                                                                                                                                    
ccGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATAtt                                                                                                                                                      >  1:1465773/1‑150 (MQ=255)
  ggTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGt                                                                                                                                                    <  2:1400440/150‑1 (MQ=255)
  ggTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGt                                                                                                                                                    <  2:1575024/150‑1 (MQ=255)
  ggTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGt                                                                                                                                                    <  1:1578065/150‑1 (MQ=255)
   gtgtGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTc                                                                                                                                                   <  1:798759/150‑1 (MQ=255)
     gtgAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCat                                                                                                                                                 <  1:920285/150‑1 (MQ=255)
      tgAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCata                                                                                                                                                <  1:1101872/150‑1 (MQ=255)
       gAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTAACCTCATTCGCTATATATTGTCatat                                                                                                                                               >  2:444177/1‑150 (MQ=255)
           aGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAg                                                                                                                                           >  2:1132630/1‑150 (MQ=255)
           aGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGGCATATATAg                                                                                                                                           <  1:1535303/150‑1 (MQ=255)
            gCCTAATTCAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGc                                                                                                                                          <  1:192572/150‑1 (MQ=255)
            gCCTAATACAAAACCGCGCTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGc                                                                                                                                          <  1:255619/150‑1 (MQ=255)
             ccTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGTCATTCGTTGCTTTTAATCCCGCCATTAAGACTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCg                                                                                                                                         <  2:1246727/150‑1 (MQ=255)
             ccTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATGACAATATATAGCg                                                                                                                                         <  2:407421/150‑1 (MQ=255)
             ccTAATACAAAAACGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCTCACTGTCGGCTTGCGTTGCTTTTAATCACGCCATTAAGCCTTTACCAAAAAGGTTATTCATCGTTCACCTCATTCGCTATAAATGACAATATATAGCg                                                                                                                                         <  2:1041789/150‑1 (MQ=255)
              cTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATGACAATATATAGCGa                                                                                                                                        <  2:1220003/150‑1 (MQ=255)
               tAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGat                                                                                                                                       >  1:633421/1‑150 (MQ=255)
                aaTACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGata                                                                                                                                      <  1:1355348/150‑1 (MQ=255)
                 aTACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGatat                                                                                                                                     >  2:808844/1‑150 (MQ=255)
                 aTACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGatat                                                                                                                                     <  1:1499654/150‑1 (MQ=255)
                  tACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATAtt                                                                                                                                    <  2:1157790/150‑1 (MQ=255)
                    cAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATAtttt                                                                                                                                  >  2:1027893/1‑150 (MQ=255)
                      aaaCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAg                                                                                                                                >  1:823939/1‑150 (MQ=255)
                       aaCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAgg                                                                                                                               <  2:533983/150‑1 (MQ=255)
                       aaCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAgg                                                                                                                               >  1:381404/1‑150 (MQ=255)
                       aaCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAgg                                                                                                                               <  2:160772/150‑1 (MQ=255)
                       aaCCGCGTTTATAATCGGCACAGAATTTTGTCAAATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAgg                                                                                                                               <  2:23271/150‑1 (MQ=255)
                             gTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGa                                                                                                                         <  2:629846/150‑1 (MQ=255)
                                tataATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACg                                                                                                                      <  1:1200101/150‑1 (MQ=255)
                                  taATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCTCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGAc                                                                                                                    >  1:313757/1‑150 (MQ=255)
                                  taATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGATATTCATCGTTCGCCTCATACGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGAc                                                                                                                    >  1:1156353/1‑150 (MQ=255)
                                     tCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACttt                                                                                                                 <  2:921270/150‑1 (MQ=255)
                                         cacaGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTgcg                                                                                                             >  1:374337/1‑150 (MQ=255)
                                         cacaGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTgcg                                                                                                             >  1:135333/1‑150 (MQ=255)
                                             gAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTAAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGAGATTTTAGGCAAAGAACGACTTTTGCGCACg                                                                                                         >  1:261878/1‑150 (MQ=255)
                                              aaTTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGc                                                                                                        <  1:748942/150‑1 (MQ=255)
                                              aaTTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGc                                                                                                        >  2:928452/1‑150 (MQ=255)
                                                     tCACATTAACCGCACTGTCGGAATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTAttttt                                                                                                 <  2:135901/150‑1 (MQ=255)
                                                      cacaTTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTAtttttt                                                                                                <  1:796887/150‑1 (MQ=255)
                                                           tAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGc                                                                                           >  2:435365/1‑150 (MQ=255)
                                                             aCCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCtt                                                                                         >  1:555575/1‑150 (MQ=255)
                                                               cGCACTGTCGGCATGCGATGCTTTTAATCCCGCCATTAAGCCTTTACAAAAATGGTTATTCATCGTTCGCCTCATTCGCTATATATTGTCATATATAGCGAGATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCAAGCTTGt                                                                                       >  2:1194830/1‑150 (MQ=255)
                                                                 cACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGttt                                                                                     <  2:675531/150‑1 (MQ=255)
                                                                 cACTGTCGGCATGAGTTGCTTTTAATCACGCCTTTAAGCCTTTACCAATATGGTTATTCATCGTTCACCTCATTCGCTATATATTTTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGttt                                                                                     <  1:106095/150‑1 (MQ=255)
                                                                  aCTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTa                                                                                    <  1:1296439/150‑1 (MQ=255)
                                                                     gTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGa                                                                                 >  2:934409/1‑150 (MQ=255)
                                                                       cGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGTCTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACg                                                                               <  1:444177/150‑1 (MQ=255)
                                                                         gCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTa                                                                             >  2:439584/1‑150 (MQ=255)
                                                                             tcGTTGCTTTTAATCACGCCATTAAGCCTTTACGAAAATGTTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATcc                                                                         <  1:73320/149‑1 (MQ=255)
                                                                               gTTTCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATAATGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCg                                                                       <  1:461953/150‑1 (MQ=255)
                                                                               gTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCg                                                                       <  2:680981/150‑1 (MQ=255)
                                                                                ttGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGt                                                                      <  2:374337/150‑1 (MQ=255)
                                                                                ttGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGt                                                                      <  1:808844/150‑1 (MQ=255)
                                                                                 tGCTTTTAATCCAGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTa                                                                     <  1:1152115/150‑1 (MQ=255)
                                                                                  gCTTTTAATCCCGCCATTACGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGACATATATAGCGATATTTTAGGCTAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTaa                                                                    >  2:858695/1‑150 (MQ=255)
                                                                                  gCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTaa                                                                    >  1:1727293/1‑150 (MQ=255)
                                                                                   cTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAag                                                                   <  2:342206/150‑1 (MQ=255)
                                                                                    ttttAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAaga                                                                  <  1:945787/150‑1 (MQ=255)
                                                                                     tttAATCCCGCCATTAATCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAagag                                                                 <  1:1543496/150‑1 (MQ=255)
                                                                                      ttAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAgaga                                                                <  1:411689/150‑1 (MQ=255)
                                                                                        aaTCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATa                                                              >  1:669124/1‑150 (MQ=255)
                                                                                          tCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATaaa                                                            <  1:395093/150‑1 (MQ=255)
                                                                                           cccGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAAc                                                           >  1:674973/1‑150 (MQ=255)
                                                                                           cccGCCATTAAGCCTTTACAAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAGAGATAAAcc                                                          <  2:1108170/150‑1 (MQ=255)
                                                                                            ccGCCATTAAGCCTTTACCAAAAAGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAAcc                                                          <  2:278961/150‑1 (MQ=255)
                                                                                                cATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTTCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATc                                                      >  1:105747/1‑150 (MQ=255)
                                                                                                        cTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAATAGATAAACCTATCAGgagaga                                              <  2:897829/150‑1 (MQ=255)
                                                                                                            aCCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCTCTCTATTTTTTCATGCTTGTTTAAGACGTTATCCCGTAAGAGATAAACCTATCAGGTGAGATAtt                                          <  2:1186522/150‑1 (MQ=255)
                                                                                                            aCCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCAAGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATAtt                                          <  1:1706572/150‑1 (MQ=255)
                                                                                                             ccAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATAttt                                         >  1:693592/1‑150 (MQ=255)
                                                                                                               aaaaTGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTAt                                       <  1:19923/150‑1 (MQ=255)
                                                                                                               aaaaTGGTTATTCATCATTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTAt                                       >  2:520716/1‑150 (MQ=255)
                                                                                                                aaaTGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATc                                      <  1:1644637/150‑1 (MQ=255)
                                                                                                                aaaTGGTTATTCATCGTTCACCTCATGCGCTTTATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATc                                      <  1:919055/150‑1 (MQ=255)
                                                                                                                  aTGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGa                                    >  2:736988/1‑150 (MQ=255)
                                                                                                                  aTGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGa                                    >  2:50017/1‑150 (MQ=255)
                                                                                                                    ggTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAAc                                  <  1:1048991/150‑1 (MQ=255)
                                                                                                                     gTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAAcc                                 >  2:1260639/1‑150 (MQ=255)
                                                                                                                     gTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAAcc                                 >  2:1027321/1‑150 (MQ=255)
                                                                                                                        aTTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCAtt                              <  1:799257/150‑1 (MQ=255)
                                                                                                                        aTTCATCGTTAACCTCATTCGCTATATATTGTAATATATAGCGATATTTTAGTAAAAGAACGACTTTTTCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCAtt                              <  1:81562/150‑1 (MQ=255)
                                                                                                                          tCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTc                            <  1:928452/150‑1 (MQ=255)
                                                                                                                           cATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCa                           >  1:528597/1‑150 (MQ=255)
                                                                                                                                       cATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGcc               >  1:231145/1‑150 (MQ=255)
                                                                                                                                        aTTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCa              <  2:773074/150‑1 (MQ=255)
                                                                                                                                         atCGCTATATAATGTCAAATTAAGCGATATTTTAGGCAAAGAACGCCTAAAGCGCTCTCTATTTTTACCGCCTTGTATAAGACGTAAACCCGTAAGAGCTCAACCTATAAGGAGAGTGATTTATCGAACCATTTCAGTCTCCTTTGACAg             >  1:285006/2‑150 (MQ=255)
                                                                                                                                          tCGCTATATATGACAATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAgg            >  1:460538/1‑150 (MQ=255)
                                                                                                                                           cGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGc           >  1:1384613/1‑150 (MQ=255)
                                                                                                                                           cGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGc           <  2:669124/150‑1 (MQ=255)
                                                                                                                                            gCTATATATGACAATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAgg                                                   <  2:1697017/109‑1 (MQ=255)
                                                                                                                                            gCTATATATGACAATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAgg                                                   >  1:1697017/1‑109 (MQ=255)
                                                                                                                                            gCTATATATGACAATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGCACCATTTCAGTGTCCTTTGCCAGGCa          >  2:736855/1‑150 (MQ=255)
                                                                                                                                            gCTATATATGACAATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCa          >  2:1647096/1‑150 (MQ=255)
                                                                                                                                            gCTATATATGACAATATATAGCGATATTTTAGACAAAGAACGACTTTAGCGCACGCTATTTTTTCATGATTGTTTAAGACGTAATACCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCGTTTGCCAGGCa          >  2:192711/1‑150 (MQ=255)
                                                                                                                                             cTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCaa         <  2:1465773/150‑1 (MQ=255)
                                                                                                                                                  tatTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATcg    >  2:1126785/1‑150 (MQ=255)
                                                                                                                                                   atTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTc                            >  2:48973/1‑125 (MQ=255)
                                                                                                                                                   atTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTc                            <  1:48973/125‑1 (MQ=255)
                                                                                                                                                     tGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGc                >  1:299363/1‑135 (MQ=255)
                                                                                                                                                     tGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGc                <  2:299363/135‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCGGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATCGGC  >  NC_000913/3146612‑3146909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: