Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,155,230 T→G 8.5% intergenic (‑12/‑125) yqhC ← / → yqhD transcriptional activator of yqhD/aldehyde reductase, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,155,2300TG 8.5% 353.8 / 19.2 130intergenic (‑12/‑125)yqhC/yqhDtranscriptional activator of yqhD/aldehyde reductase, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base T (54/65);  new base G (5/6);  total (59/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-01

Only 100 of 134 total aligned reads displayed.

AGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGCACACC  >  NC_000913/3155082‑3155378
                                                                                                                                                    |                                                                                                                                                    
aGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGTAATAATGCGGCAATTTGATTGTGCGAAATTTTGTAGCATTTCTCCAGCActc                                                                                                                                                     >  1:449557/1‑150 (MQ=255)
   ccTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTgg                                                                                                                                                  <  1:250868/150‑1 (MQ=255)
     tGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGgag                                                                                                                                                <  2:1550741/150‑1 (MQ=255)
      gACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGgaga                                                                                                                                               <  1:297870/150‑1 (MQ=255)
      gACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGgaga                                                                                                                                               >  1:1452197/1‑150 (MQ=255)
         aaaCTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGAGTGCTGGAGAAAt                                                                                                                                            <  1:818559/150‑1 (MQ=255)
          agcTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATa                                                                                                                                           <  1:1527191/148‑1 (MQ=255)
          aaCTATATTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCACGCGGCAAATCTCTTCACGTGTCATATCGCGTAATTTCTTAGGATTAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTACAGCACTCTGTAGAAATa                                                                                                                                           >  2:301562/1‑150 (MQ=255)
          aaCTATATTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTATCAAATCGCGTAATTTCTTAGGAATAATGCGGCAAATTGATTGTGCTCAATTTTGTAGCATTTCTCCAGAGTGCTGGAGAAAtg                                                                                                                                           <  1:447220/150‑2 (MQ=255)
             tATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGAGTGCTGGAGAAAtgcta                                                                                                                                        <  1:993295/150‑5 (MQ=255)
              aTTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCa                                                                                                                                       <  1:605183/150‑1 (MQ=255)
               ttttCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCaa                                                                                                                                      <  1:1362652/150‑1 (MQ=255)
                tttCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGTATTTCTCCAGAGTGCTGGAGAAAtgctacaa                                                                                                                                     <  1:133690/150‑8 (MQ=255)
                tttCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAg                                                                                                                                     >  2:1476571/1‑150 (MQ=255)
                tttCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTGCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAg                                                                                                                                     <  2:498093/150‑1 (MQ=255)
                      tATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTg                                                                                                                               >  2:435554/1‑150 (MQ=255)
                        tttttCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGc                                                                                                                             <  1:852672/150‑1 (MQ=255)
                          tttCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAg                                                                                                                           <  1:1116205/150‑1 (MQ=255)
                          tttCAGTTTATTAACATTATCCGCCCGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAg                                                                                                                           <  2:1087424/150‑1 (MQ=255)
                           ttCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGa                                                                                                                          <  2:835963/150‑1 (MQ=255)
                             cAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGaaa                                                                                                                        >  1:759567/1‑150 (MQ=255)
                             cAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGaaa                                                                                                                        >  2:1708418/1‑150 (MQ=255)
                               gTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATg                                                                                                                      <  2:1124171/150‑1 (MQ=255)
                                  tattaACTTTATCAGCAAGCAATATGCAAATCGCTTAACGTTTCATATCGCGTTATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGAAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGc                                                                                                                   <  1:996382/150‑1 (MQ=255)
                                   attaACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCa                                                                                                                  <  2:1083974/150‑1 (MQ=255)
                                   attaACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCa                                                                                                                  >  1:1327534/1‑150 (MQ=255)
                                    ttaACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCAt                                                                                                                 <  1:924296/150‑1 (MQ=255)
                                    ttaACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCAt                                                                                                                 <  1:247885/150‑1 (MQ=255)
                                      aaCTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATCTCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTg                                                                                                               <  2:858983/150‑1 (MQ=255)
                                      aaCTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTg                                                                                                               >  2:1143569/1‑150 (MQ=255)
                                       aCTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTgt                                                                                                              >  1:605246/1‑149 (MQ=255)
                                       aCTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTga                                                                                                              <  1:959313/150‑1 (MQ=255)
                                       aCTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTga                                                                                                              >  2:1400445/1‑150 (MQ=255)
                                       aCTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCAAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTga                                                                                                              >  1:863715/1‑150 (MQ=255)
                                        cTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTgag                                                                                                             >  1:1053761/1‑150 (MQ=255)
                                        cTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTgag                                                                                                             >  2:335373/1‑150 (MQ=255)
                                          ttATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGagag                                                                                                           <  1:1054826/150‑1 (MQ=255)
                                               cGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAgg                                                                                                      >  1:806305/1‑150 (MQ=255)
                                                  cAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGgcg                                                                                                   <  2:1511629/150‑1 (MQ=255)
                                                       aGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGc                                                                                              <  2:1221853/150‑1 (MQ=255)
                                                       aGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTAAGGAATAATGCGGCAAATTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGc                                                                                              <  2:570399/150‑1 (MQ=255)
                                                        gCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCg                                                                                             <  2:815504/150‑1 (MQ=255)
                                                         cGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGa                                                                                            <  2:1231515/150‑1 (MQ=255)
                                                           gCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATc                                                                                          <  2:1364806/150‑1 (MQ=255)
                                                           gCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATc                                                                                          <  2:1109078/150‑1 (MQ=255)
                                                            cAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATca                                                                                         >  2:1414496/1‑150 (MQ=255)
                                                               aTCTCTTCCCGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAAGGAGCATTGAGAGCCAGGGCGCTGGCGATCACaa                                                                                      <  2:1328964/150‑1 (MQ=255)
                                                               aTCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAATTGAGCATTGAGAGCCAGGGCGCTGGCGATCACaa                                                                                      <  1:435554/150‑1 (MQ=255)
                                                                tctcTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAAt                                                                                     <  2:414407/150‑1 (MQ=255)
                                                                   ctTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGaa                                                                                  >  1:1093788/1‑150 (MQ=255)
                                                                   ctTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGCCAATTTGATTGTGCGCAATTTTGTAGCCTTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAACTGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGaa                                                                                  <  1:273348/150‑1 (MQ=255)
                                                                    ttCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGGAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGaaa                                                                                 <  2:1459824/150‑1 (MQ=255)
                                                                    ttCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGaaa                                                                                 >  2:1457997/1‑150 (MQ=255)
                                                                        cGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACa                                                                             >  2:1115127/1‑150 (MQ=255)
                                                                         gTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACAt                                                                            <  1:335373/150‑1 (MQ=255)
                                                                          tttCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGAATGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATc                                                                           <  2:257118/150‑1 (MQ=255)
                                                                             cATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAgg                                                                        >  1:1393818/1‑150 (MQ=255)
                                                                                  cgcgTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGAt                                                                   >  1:1029208/1‑150 (MQ=255)
                                                                                   gcgTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATc                                                                  >  1:870980/1‑150 (MQ=255)
                                                                                   gcgTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATc                                                                  >  2:443434/1‑150 (MQ=255)
                                                                                    cgTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCg                                                                 <  2:450062/150‑1 (MQ=255)
                                                                                         tttCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTtctc                                                            <  2:449557/150‑1 (MQ=255)
                                                                                            cTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATTAAAAACATCAGGCAGATCGTTCTCTGc                                                         >  1:149517/1‑150 (MQ=255)
                                                                                            cTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACAACAGGCAGATCGTTCTCTGc                                                         >  1:147485/1‑150 (MQ=255)
                                                                                             ttAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGcc                                                        <  2:1266114/150‑1 (MQ=255)
                                                                                                ggAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTc                                                     >  1:1210606/1‑150 (MQ=255)
                                                                                                ggAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTc                                                     >  2:597693/1‑150 (MQ=255)
                                                                                                 gAATTATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCa                                                    <  2:176301/150‑1 (MQ=255)
                                                                                                 gAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATTGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCa                                                    >  1:585902/1‑150 (MQ=255)
                                                                                                         cGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGcc                                            >  2:1623764/1‑150 (MQ=255)
                                                                                                          ggCATTTTGTTTGTGCGTATTTTTGTTGCTTTTCTACATAACTCTGGATAAATAGGCAAGACATTGGCATATATGTTATTTGAGAGCCATGGCGCTTGCGATCTCAATGAAATATATCAGGCAGATCGTTCTCTGCCCTCATATTGGgcc                                           <  2:1687682/150‑1 (MQ=255)
                                                                                                           gCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGATCTCTGCCCTCATATTGGCCCa                                          >  1:764372/1‑150 (MQ=255)
                                                                                                                        gcgcAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCaa                             >  1:575299/1‑150 (MQ=255)
                                                                                                                         cgcAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAg                            >  1:561155/1‑150 (MQ=255)
                                                                                                                            aaTTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTaa                         >  1:699869/1‑150 (MQ=255)
                                                                                                                                ttGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGaa                     <  2:1093788/150‑1 (MQ=255)
                                                                                                                                ttGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGaa                     >  1:814019/1‑150 (MQ=255)
                                                                                                                                cttgcctaTTTCTCCAGAGTGCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTTAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGaa                     >  2:676401/8‑150 (MQ=255)
                                                                                                                                 tGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGaac                    <  2:759567/150‑1 (MQ=255)
                                                                                                                                  gTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCAAATTGGCCCAGCAACGGGAGCAAGTAATGaaaa                   >  2:259380/1‑148 (MQ=255)
                                                                                                                                    cctaTTTCTCCAGAGTGCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGaacaac                 >  1:353633/4‑150 (MQ=255)
                                                                                                                                    cctaTTTCTCCAGAGTGCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGaacaac                 >  1:1690377/4‑150 (MQ=255)
                                                                                                                                      cATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACtt               >  2:1534872/1‑150 (MQ=255)
                                                                                                                                      cATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACtt               <  1:1476571/150‑1 (MQ=255)
                                                                                                                                       aTTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACttt              <  1:863054/150‑1 (MQ=255)
                                                                                                                                       aTTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACttt              <  2:699869/150‑1 (MQ=255)
                                                                                                                                       aTTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACttt              <  2:693836/150‑1 (MQ=255)
                                                                                                                                        tttCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTa             >  1:183835/1‑150 (MQ=255)
                                                                                                                                        tttCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTa             <  2:1263936/150‑1 (MQ=255)
                                                                                                                                        tttCTCCAGAGTGCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTa             >  2:784247/1‑150 (MQ=255)
                                                                                                                                           ctcCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATc          <  1:1708418/150‑1 (MQ=255)
                                                                                                                                           ctcCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATc          <  1:1143569/150‑1 (MQ=255)
                                                                                                                                            tcCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCt         <  2:575299/150‑1 (MQ=255)
                                                                                                                                             ccAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTg        >  2:455635/1‑150 (MQ=255)
                                                                                                                                             ccAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTg        >  1:1416105/1‑150 (MQ=255)
                                                                                                                                              cAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGc       <  1:1574872/150‑1 (MQ=255)
                                                                                                                                              cAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGc       <  1:1228335/150‑1 (MQ=255)
                                                                                                                                                gCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGcac     <  1:1573429/150‑1 (MQ=255)
                                                                                                                                                gCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGcac     <  1:109368/150‑1 (MQ=255)
                                                                                                                                                   ctctGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGCACAcc  >  1:680382/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGCACACC  >  NC_000913/3155082‑3155378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: