Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,166,154 T→C 5.0% Q522R (CAG→CGG) ‡ ygiS ← putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,166,1540TC 5.0% 370.8 / 6.2 119Q522R (CAG→CGG) ‡ygiSputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (63/50);  new base C (4/2);  total (67/52)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

TCAAAGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCTTTGTCGCATCAGTGAT  >  NC_000913/3166006‑3166299
                                                                                                                                                    |                                                                                                                                                  
tCAAAGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTg                                                                                                                                                   <  2:174532/150‑1 (MQ=255)
tCAAAGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTg                                                                                                                                                   >  2:64424/1‑150 (MQ=255)
 cAAAGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGTCTAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGCCGTGCTCCGACGGCGATAAAGGCATCAATCAGCCTTGAAATACAACTCATTGCTGTAGACATAATCCTgg                                                                                                                                                  >  2:637551/1‑150 (MQ=255)
 cAAAGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTgg                                                                                                                                                  >  2:65435/1‑150 (MQ=255)
  aaaGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTggg                                                                                                                                                 <  1:1671072/150‑1 (MQ=255)
   aaGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGTCAAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTgggg                                                                                                                                                <  2:1578688/150‑1 (MQ=255)
       ggCAATATTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTa                                                                                                                                            >  2:1426915/1‑150 (MQ=255)
          aaTATTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCATTGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGc                                                                                                                                         <  2:1303778/150‑1 (MQ=255)
          aaTATTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGc                                                                                                                                         <  1:706278/150‑1 (MQ=255)
            tatTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAg                                                                                                                                       <  2:886758/150‑1 (MQ=255)
            tatTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCCCAGGATTATgtct                                                                                                                                       <  1:1710288/150‑4 (MQ=255)
            tatTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCCCAGGATTATgtct                                                                                                                                       <  1:1703602/150‑4 (MQ=255)
             atTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGc                                                                                                                                      <  1:457593/150‑1 (MQ=255)
                 ccGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGaa                                                                                                                                  <  1:220016/150‑1 (MQ=255)
                   ggAGATACTACATTATCTGCCGCGTCGAGTCACAGGATATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACTGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGaaaa                                                                                                                                <  2:1130635/150‑1 (MQ=255)
                     agaTACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATATGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAAcc                                                                                                                              >  1:1218686/1‑150 (MQ=255)
                        tactacATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGcc                                                                                                                           >  1:1601674/1‑150 (MQ=255)
                              aTTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTa                                                                                                                     <  2:752474/150‑1 (MQ=255)
                              aTTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTa                                                                                                                     >  1:616693/1‑150 (MQ=255)
                               ttATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGCCATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAg                                                                                                                   >  2:62343/1‑150 (MQ=255)
                                  tCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACACCTCTTTGCTGTAGACATAATCCTGGGGATTATGAAGCGGAAAACCGCCAACGTAGGGt                                                                                                                >  1:1106921/1‑150 (MQ=255)
                                   cTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGtt                                                                                                               >  1:1483931/1‑150 (MQ=255)
                                    tGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGttt                                                                                                              <  2:89156/150‑1 (MQ=255)
                                     tccGCTTCGAGTCACAGGATATCAACGTGAATAGCGCCGCCGGACTTGCTCCGACGGCGATAAAGGCATAAATTTGCCTTGATATACAACTATTTGCTGTAGAAATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTa                                                                                                             <  1:1047353/149‑1 (MQ=255)
                                        gcgTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGc                                                                                                          <  1:342501/150‑1 (MQ=255)
                                        gcgTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCGCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGc                                                                                                          <  1:354894/150‑1 (MQ=255)
                                            cGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGtt                                                                                                      <  2:383928/150‑1 (MQ=255)
                                             gAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGttt                                                                                                     <  2:1062838/150‑1 (MQ=255)
                                             gAGGCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGttt                                                                                                     <  2:1489489/150‑1 (MQ=255)
                                              aGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTg                                                                                                    <  1:1613612/150‑1 (MQ=255)
                                               gTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGa                                                                                                   >  1:1449604/1‑150 (MQ=255)
                                               gTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGa                                                                                                   >  1:1165833/1‑150 (MQ=255)
                                                tCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGAt                                                                                                  <  1:1170119/150‑1 (MQ=255)
                                                 cacaGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGAtt                                                                                                 >  2:362456/1‑150 (MQ=255)
                                                 cacaGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGAtt                                                                                                 >  1:511560/1‑150 (MQ=255)
                                                    aGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAAc                                                                                              <  1:1154447/150‑1 (MQ=255)
                                                        gaTCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCt                                                                                          >  1:477788/1‑150 (MQ=255)
                                                        gaTCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCt                                                                                          <  1:655653/150‑1 (MQ=255)
                                                         ctCAACGTGTATTCCGCCTCCGGAAGTTCTCCTACGTAGATAAAGGCATAAATGTGCCTTGATATCCAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTg                                                                                         <  2:1140021/149‑1 (MQ=255)
                                                           cAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGAt                                                                                       >  2:843376/1‑150 (MQ=255)
                                                            aaCGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCCGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGAta                                                                                      >  2:375624/1‑150 (MQ=255)
                                                            aaCGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGAta                                                                                      >  1:955488/1‑150 (MQ=255)
                                                             aCGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGAtag                                                                                     >  1:1143838/1‑150 (MQ=255)
                                                             aCGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGAtag                                                                                     <  2:1724841/150‑1 (MQ=255)
                                                                tGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGAtagtag                                                                                  <  2:1725274/150‑1 (MQ=255)
                                                                 gAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGa                                                                                 >  2:1115026/1‑150 (MQ=255)
                                                                     aGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAgg                                                                             >  2:275248/1‑150 (MQ=255)
                                                                       cgccgcCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACAAAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGaa                                                                           <  1:445251/150‑1 (MQ=255)
                                                                           gccgGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAg                                                                       >  1:818365/1‑150 (MQ=255)
                                                                            ccgGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGc                                                                      <  1:65435/150‑1 (MQ=255)
                                                                             cgGACGCGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGAGAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCg                                                                     >  2:871429/1‑150 (MQ=255)
                                                                                 cGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGc                                                                 >  2:195742/1‑150 (MQ=255)
                                                                                  gTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGcc                                                                >  2:922140/1‑150 (MQ=255)
                                                                                  gTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGcc                                                                <  1:843376/150‑1 (MQ=255)
                                                                                   tGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCt                                                               >  1:602944/1‑150 (MQ=255)
                                                                                    gCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTg                                                              >  1:1535242/1‑150 (MQ=255)
                                                                                     cTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGAAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTgt                                                             >  1:943280/1‑150 (MQ=255)
                                                                                      tCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTgtt                                                            >  2:164859/1‑150 (MQ=255)
                                                                                       ccGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTgtt                                                            >  1:1494290/1‑150 (MQ=255)
                                                                                       ccGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTgttg                                                           <  1:1641375/150‑1 (MQ=255)
                                                                                       ccGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTgttg                                                           <  2:25011/150‑1 (MQ=255)
                                                                                           cGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTgttggttg                                                       >  1:1209378/1‑150 (MQ=255)
                                                                                             gCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTtgat                                                     <  2:798851/150‑1 (MQ=255)
                                                                                             gCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGAAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTtgat                                                     <  2:75662/150‑1 (MQ=255)
                                                                                              cGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCCAGTTAGGGTTTAAGCAGTTTGATTAACGTCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTtgatg                                                    >  1:127160/1‑150 (MQ=255)
                                                                                              cGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTtgatg                                                    >  1:874461/1‑150 (MQ=255)
                                                                                              cGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTtgatg                                                    <  2:489251/150‑1 (MQ=255)
                                                                                              cGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGACAACGTAGGGTTTAAGCAGTTTGATAAACGGCTGATAGTTGATAGTAATCAGCGTTGCCTGTTGGTtgatg                                                    >  2:1640639/1‑150 (MQ=255)
                                                                                                 tAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATc                                                 <  2:448870/150‑1 (MQ=255)
                                                                                                  aaaGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCa                                                <  1:1365587/150‑1 (MQ=255)
                                                                                                  aaaGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCa                                                <  1:661510/150‑1 (MQ=255)
                                                                                                       cATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAATCCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTTATAGTAGATAGGAATCAGCGGTTCCTGTTGGTTGATGTTCACTTct                                           >  2:78531/1‑150 (MQ=255)
                                                                                                          cAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTctgcc                                        >  1:560595/1‑150 (MQ=255)
                                                                                                          cAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTctgcc                                        <  2:813743/150‑1 (MQ=255)
                                                                                                                 ccTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGAt                                 >  2:1506872/1‑150 (MQ=255)
                                                                                                                 ccTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGAt                                 >  1:738577/1‑150 (MQ=255)
                                                                                                                  cTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATa                                >  1:1441503/1‑150 (MQ=255)
                                                                                                                     gATATACAACTCTTTGCTGTAGGCATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACaa                             <  2:1005541/150‑1 (MQ=255)
                                                                                                                     gATATACAACTCTTTGCTGTAGACATAATCCTGGGGAGTATGCAGCGGAAAACCGCAAACGTAGGGTTTAAGCAGTTTGATTAAGGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACaa                             >  2:669680/1‑150 (MQ=255)
                                                                                                                       tataCAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACg                           >  1:338389/1‑150 (MQ=255)
                                                                                                                       tataCAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACg                           >  2:654031/1‑150 (MQ=255)
                                                                                                                           cAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTACCGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCAGATACAACGCAtt                       >  2:299667/1‑150 (MQ=255)
                                                                                                                           cAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCAtt                       >  2:738384/1‑150 (MQ=255)
                                                                                                                               tctTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGGTTAAGCCGTTTGAGTAACGGCTGAGTGTAGAGAGGACTCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGc                   >  2:172056/1‑150 (MQ=255)
                                                                                                                                ctTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCt                  >  2:635099/1‑150 (MQ=255)
                                                                                                                                ctTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCt                  >  1:823566/1‑150 (MQ=255)
                                                                                                                                 tttGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCtt                 <  2:204125/150‑1 (MQ=255)
                                                                                                                                 tttGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCtt                 <  2:1449604/150‑1 (MQ=255)
                                                                                                                                     cTGTAGACATAATCCTGGTGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCTTTGTc             <  1:458476/150‑1 (MQ=255)
                                                                                                                                       gTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCTTTGTCGc           >  2:1180671/1‑150 (MQ=255)
                                                                                                                                       gTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGACAACGTAGGCTTTAAGCAGTTTGAATAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAAAGCATTACGCTTTGTCGc           >  2:1214736/1‑150 (MQ=255)
                                                                                                                                       cgctgcATAATCCCCAGGATTATGTAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCTTTGTCGc           >  1:1187529/6‑150 (MQ=255)
                                                                                                                                        gctgcATAATCCCCAGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCTTTGTCGCa          >  1:1521216/5‑150 (MQ=255)
                                                                                                                                        gctgcATAATCCCCAGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCTTTGTCGCa          >  1:1128885/5‑150 (MQ=255)
                                                                                                                                         ctgcATAATCCCCAGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCTTTGTCGCAt         >  1:425303/4‑150 (MQ=255)
                                                                                                                                           aCATAATCCTGGGGATTATGCAGCGGAAAACCTCCAACGTAGGGTTTAATCAGTTTGATTAACGGCTGATAGTAGATAGGTATCAGCGGTGCCTGTTTGTTGATGATCACTTCTGCCGGCTGATTCAACGCATTACGCTTTGTCGCATCa       >  2:210005/1‑150 (MQ=255)
                                                                                                                                           aCATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCCCTTCTGCCTGCTGATACAACGCATTACGCTTTGTCGCATCa       >  1:1355284/1‑150 (MQ=255)
                                                                                                                                           aCATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCTTTGTCGCATCa       >  1:202770/1‑150 (MQ=255)
                                                                                                                                              tAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCTTTGTCGCATCAGTg    >  2:57532/1‑150 (MQ=255)
                                                                                                                                                aTCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTTCCTGCTGATACAACGCATTACGCTTTGTCGCATCAGTGAt  >  1:113191/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TCAAAGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCTTTGTCGCATCAGTGAT  >  NC_000913/3166006‑3166299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: