Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,166,156 G→A 5.3% P521P (CCC→CCT ygiS ← putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,166,1560GA 5.3% 359.2 / 4.7 112P521P (CCC→CCTygiSputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (60/46);  new base A (4/2);  total (64/48)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

Only 100 of 119 total aligned reads displayed.

CAAAGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCTTTGTCGCATCAGTGATCTGCGT  >  NC_000913/3166007‑3166305
                                                                                                                                                     |                                                                                                                                                      
cAAAGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTgg                                                                                                                                                        >  2:65435/1‑150 (MQ=255)
 aaaGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTggg                                                                                                                                                       <  1:1671072/150‑1 (MQ=255)
  aaGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTgggg                                                                                                                                                      <  2:17419/150‑1 (MQ=255)
  aaGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGTCAAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTgggg                                                                                                                                                      <  2:1578688/150‑1 (MQ=255)
     gggCAATATTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGAtt                                                                                                                                                   <  2:643895/150‑1 (MQ=255)
      ggCAATATTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTa                                                                                                                                                  >  2:1426915/1‑150 (MQ=255)
         aaTATTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCATTGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGc                                                                                                                                               <  2:1303778/150‑1 (MQ=255)
         aaTATTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGc                                                                                                                                               <  1:706278/150‑1 (MQ=255)
           tatTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAg                                                                                                                                             <  2:886758/150‑1 (MQ=255)
           tatTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCCCAGGATTATgtct                                                                                                                                             <  1:1703602/150‑4 (MQ=255)
           tatTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCCCAGGATTATgtct                                                                                                                                             <  1:1710288/150‑4 (MQ=255)
            atTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGc                                                                                                                                            <  1:457593/150‑1 (MQ=255)
                ccGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGaa                                                                                                                                        <  1:220016/150‑1 (MQ=255)
                  ggAGATACTACATTATCTGCCGCGTCGAGTCACAGGATATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACTGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGaaaa                                                                                                                                      <  2:1130635/150‑1 (MQ=255)
                    agaTACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATATGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAAcc                                                                                                                                    >  1:1218686/1‑150 (MQ=255)
                       tactacATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGcc                                                                                                                                 >  1:1601674/1‑150 (MQ=255)
                             aTTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTa                                                                                                                           <  2:752474/150‑1 (MQ=255)
                             aTTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTa                                                                                                                           >  1:426991/1‑150 (MQ=255)
                             aTTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTa                                                                                                                           >  1:616693/1‑150 (MQ=255)
                              ttATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGCCATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAg                                                                                                                         >  2:62343/1‑150 (MQ=255)
                                aTCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAggg                                                                                                                       <  1:554648/150‑1 (MQ=255)
                                 tCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACACCTCTTTGCTGTAGACATAATCCTGGGGATTATGAAGCGGAAAACCGCCAACGTAGGGt                                                                                                                      >  1:1106921/1‑150 (MQ=255)
                                  cTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGtt                                                                                                                     >  1:1483931/1‑150 (MQ=255)
                                   tGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGttt                                                                                                                    <  2:89156/150‑1 (MQ=255)
                                    tccGCTTCGAGTCACAGGATATCAACGTGAATAGCGCCGCCGGACTTGCTCCGACGGCGATAAAGGCATAAATTTGCCTTGATATACAACTATTTGCTGTAGAAATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTa                                                                                                                   <  1:1047353/149‑1 (MQ=255)
                                       gcgTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGc                                                                                                                <  1:342501/150‑1 (MQ=255)
                                       gcgTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCGCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGc                                                                                                                <  1:354894/150‑1 (MQ=255)
                                           cGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCCGCGGAAAACCGCCAACGTAGGGTTTAAGCAGtt                                                                                                            >  2:1085788/1‑150 (MQ=255)
                                            gAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGttt                                                                                                           <  2:1062838/150‑1 (MQ=255)
                                            gAGGCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGttt                                                                                                           <  2:1489489/150‑1 (MQ=255)
                                             aGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTg                                                                                                          <  1:1613612/150‑1 (MQ=255)
                                              gTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGa                                                                                                         >  1:1449604/1‑150 (MQ=255)
                                              gTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGa                                                                                                         >  1:1165833/1‑150 (MQ=255)
                                               tCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGAt                                                                                                        <  1:1170119/150‑1 (MQ=255)
                                                cacaGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGAtt                                                                                                       >  2:362456/1‑150 (MQ=255)
                                                   aGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAAc                                                                                                    <  1:1154447/150‑1 (MQ=255)
                                                       gaTCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCt                                                                                                >  1:477788/1‑150 (MQ=255)
                                                       gaTCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCt                                                                                                <  1:655653/150‑1 (MQ=255)
                                                        ctCAACGTGTATTCCGCCTCCGGAAGTTCTCCTACGTAGATAAAGGCATAAATGTGCCTTGATATCCAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTg                                                                                               <  2:1140021/149‑1 (MQ=255)
                                                          cAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGAt                                                                                             >  2:843376/1‑150 (MQ=255)
                                                           aaCGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCCGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGAta                                                                                            >  2:375624/1‑150 (MQ=255)
                                                           aaCGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGAta                                                                                            >  1:955488/1‑150 (MQ=255)
                                                            aCGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGAtag                                                                                           >  1:1143838/1‑150 (MQ=255)
                                                               tGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGAtagtag                                                                                        <  2:1725274/150‑1 (MQ=255)
                                                               tGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGGAGGGTTTAAGCAGTTTGATTAACGGCTGAtagtag                                                                                        >  1:671470/1‑150 (MQ=255)
                                                                gAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGa                                                                                       >  2:1115026/1‑150 (MQ=255)
                                                                    aGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAgg                                                                                   >  2:275248/1‑150 (MQ=255)
                                                                      cgccgcCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACAAAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGaa                                                                                 <  1:445251/150‑1 (MQ=255)
                                                                       gccgccGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAAt                                                                                >  2:126634/1‑150 (MQ=255)
                                                                          gccgGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAg                                                                             >  1:818365/1‑150 (MQ=255)
                                                                           ccgGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGc                                                                            <  1:65435/150‑1 (MQ=255)
                                                                            cgGACGCGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGAGAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCg                                                                           >  2:871429/1‑150 (MQ=255)
                                                                                cGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGc                                                                       >  2:195742/1‑150 (MQ=255)
                                                                                 gTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGcc                                                                      >  2:922140/1‑150 (MQ=255)
                                                                                 gTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGcc                                                                      <  1:843376/150‑1 (MQ=255)
                                                                                  tGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCt                                                                     >  1:602944/1‑150 (MQ=255)
                                                                                   gCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTg                                                                    >  1:1535242/1‑150 (MQ=255)
                                                                                    cTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGAAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTgt                                                                   >  1:943280/1‑150 (MQ=255)
                                                                                     tCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTgtt                                                                  >  2:164859/1‑150 (MQ=255)
                                                                                      ccGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTgtt                                                                  >  1:1494290/1‑150 (MQ=255)
                                                                                      ccGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTgttg                                                                 <  1:1641375/150‑1 (MQ=255)
                                                                                        gACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTgttggt                                                               >  2:1638483/1‑150 (MQ=255)
                                                                                          cGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTgttggttg                                                             >  1:1209378/1‑150 (MQ=255)
                                                                                            gCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTtgat                                                           <  1:86667/150‑1 (MQ=255)
                                                                                            gCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTtgat                                                           <  2:798851/150‑1 (MQ=255)
                                                                                            gCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGAAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTtgat                                                           <  2:75662/150‑1 (MQ=255)
                                                                                             cGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCCAGTTAGGGTTTAAGCAGTTTGATTAACGTCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTtgatg                                                          >  1:127160/1‑150 (MQ=255)
                                                                                             cGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTtgatg                                                          >  1:874461/1‑150 (MQ=255)
                                                                                             cGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTtgatg                                                          <  2:489251/150‑1 (MQ=255)
                                                                                             cGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGACAACGTAGGGTTTAAGCAGTTTGATAAACGGCTGATAGTTGATAGTAATCAGCGTTGCCTGTTGGTtgatg                                                          >  2:1640639/1‑150 (MQ=255)
                                                                                                tAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATc                                                       <  2:448870/150‑1 (MQ=255)
                                                                                                 aaaGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCa                                                      <  1:1365587/150‑1 (MQ=255)
                                                                                                      cATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAATCCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTTATAGTAGATAGGAATCAGCGGTTCCTGTTGGTTGATGTTCACTTct                                                 >  2:78531/1‑150 (MQ=255)
                                                                                                         cAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTctgcc                                              >  1:560595/1‑150 (MQ=255)
                                                                                                         cAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTctgcc                                              <  2:813743/150‑1 (MQ=255)
                                                                                                           aTGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGAGTTTGCAGCGGAAAACCGCCAACGTAGGGTGTAAGGAGTTTGAGTACCGGCTGATTGTAGATAGGAATCAGAGGTGGATGTTGGGTGATGATCCCTTctgcctg                                            >  2:272860/1‑150 (MQ=255)
                                                                                                                ccTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGAt                                       >  1:738577/1‑150 (MQ=255)
                                                                                                                 cTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATa                                      >  1:1441503/1‑150 (MQ=255)
                                                                                                                    gATATACAACTCTTTGCTGTAGACATAATCCTGGGGAGTATGCAGCGGAAAACCGCAAACGTAGGGTTTAAGCAGTTTGATTAAGGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACaa                                   >  2:669680/1‑150 (MQ=255)
                                                                                                                      tataCAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACg                                 >  1:338389/1‑150 (MQ=255)
                                                                                                                      tataCAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACg                                 >  2:654031/1‑150 (MQ=255)
                                                                                                                          cAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTACCGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCAGATACAACGCAtt                             >  2:299667/1‑150 (MQ=255)
                                                                                                                          cAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCAtt                             >  2:738384/1‑150 (MQ=255)
                                                                                                                              tctTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGGTTAAGCCGTTTGAGTAACGGCTGAGTGTAGAGAGGACTCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGc                         >  2:172056/1‑150 (MQ=255)
                                                                                                                               ctTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCt                        >  2:635099/1‑150 (MQ=255)
                                                                                                                                tttGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCtt                       <  2:204125/150‑1 (MQ=255)
                                                                                                                                tttGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCtt                       <  2:1449604/150‑1 (MQ=255)
                                                                                                                                 ttGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCttt                      <  2:426991/150‑1 (MQ=255)
                                                                                                                                    cTGTAGACATAATCCTGGTGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCTTTGTc                   <  1:458476/150‑1 (MQ=255)
                                                                                                                                      gTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGACAACGTAGGCTTTAAGCAGTTTGAATAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAAAGCATTACGCTTTGTCGc                 >  2:1214736/1‑150 (MQ=255)
                                                                                                                                      cgctgcATAATCCCCAGGATTATGTAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCTTTGTCGc                 >  1:1187529/6‑150 (MQ=255)
                                                                                                                                       gctgcATAATCCCCAGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCTTTGTCGCa                >  1:1128885/5‑150 (MQ=255)
                                                                                                                                       gctgcATAATCCCCAGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCTTTGTCGCa                >  1:1521216/5‑150 (MQ=255)
                                                                                                                                          aCATAATCCTGGGGATTATGCAGCGGAAAACCTCCAACGTAGGGTTTAATCAGTTTGATTAACGGCTGATAGTAGATAGGTATCAGCGGTGCCTGTTTGTTGATGATCACTTCTGCCGGCTGATTCAACGCATTACGCTTTGTCGCATCa             >  2:210005/1‑150 (MQ=255)
                                                                                                                                          aCATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCCCTTCTGCCTGCTGATACAACGCATTACGCTTTGTCGCATCa             >  1:1355284/1‑150 (MQ=255)
                                                                                                                                          aCATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCTTTGTCGCATCa             >  1:202770/1‑150 (MQ=255)
                                                                                                                                             tAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCTTTGTCGCATCAGTg          >  2:57532/1‑150 (MQ=255)
                                                                                                                                               aTCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTTCCTGCTGATACAACGCATTACGCTTTGTCGCATCAGTGAt        >  1:113191/1‑150 (MQ=255)
                                                                                                                                                    ggggATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACGACGCATTACGCTTTGTCGCATCAGTGATCTGCg   >  1:755477/1‑150 (MQ=255)
                                                                                                                                                     gggATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGACTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCTTTGTCGCATCAGTGATCTGCGt  >  1:284581/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CAAAGGGGCAATATTGCCGGAGATACTACATTATCTGCCGCGTCGAGTCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTAGGGTTTAAGCAGTTTGATTAACGGCTGATAGTAGATAGGAATCAGCGGTGCCTGTTGGTTGATGATCACTTCTGCCTGCTGATACAACGCATTACGCTTTGTCGCATCAGTGATCTGCGT  >  NC_000913/3166007‑3166305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: