Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,173,476 C→T 5.9% intergenic (+20/+28) ygiN → / ← parE quinol monooxygenase/DNA topoisomerase IV, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,173,4760CT 5.9% 429.7 / 8.9 135intergenic (+20/+28)ygiN/parEquinol monooxygenase/DNA topoisomerase IV, subunit B
Reads supporting (aligned to +/- strand):  ref base C (68/59);  new base T (4/4);  total (72/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

GATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATC  >  NC_000913/3173327‑3173623
                                                                                                                                                     |                                                                                                                                                   
gatTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGc                                                                                                                                                     <  1:1313569/150‑1 (MQ=255)
 atTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGcc                                                                                                                                                    >  2:1438570/1‑150 (MQ=255)
  ttGAGCAGTGGGAAAGCATCGCGCATCATGAAGCGCATCTGCAAACCCCCCACATGAACGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCa                                                                                                                                                   <  2:313772/150‑1 (MQ=255)
    gAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATc                                                                                                                                                 >  2:1162033/1‑150 (MQ=255)
      gCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCTGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCt                                                                                                                                               >  1:748743/1‑150 (MQ=255)
         gTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGAc                                                                                                                                            >  2:1088671/1‑150 (MQ=255)
          tGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAACGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGAcc                                                                                                                                           <  1:910242/150‑1 (MQ=255)
            ggAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCgg                                                                                                                                         <  1:705904/150‑1 (MQ=255)
            ggAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCgg                                                                                                                                         >  1:1023010/1‑150 (MQ=255)
             gAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGc                                                                                                                                       >  2:1471839/1‑150 (MQ=255)
              aaaGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGTCAGGATGGCCGGGc                                                                                                                                       <  1:465444/150‑1 (MQ=255)
                 gCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGTCAGGATGGCCGGGCaaa                                                                                                                                    <  2:441237/150‑2 (MQ=255)
                   aTCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGTCAGGATGGCCGGGCaaaca                                                                                                                                  <  1:474940/150‑4 (MQ=255)
                    tCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGtt                                                                                                                                 <  1:812130/150‑1 (MQ=255)
                     cgcgCATCTTGACGCGCATCTGCAAACCCCGCACAGGAAGGGGGATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATTAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTc                                                                                                                                <  2:270149/150‑1 (MQ=255)
                     cgcgCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTc                                                                                                                                <  1:519448/150‑1 (MQ=255)
                     cgcgCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTc                                                                                                                                >  2:171056/1‑150 (MQ=255)
                      gcgcATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGTCAGGATGGCCGGGCaaacaagg                                                                                                                               <  2:613594/150‑7 (MQ=255)
                      gcgcATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGAAATGTTCt                                                                                                                               >  1:1536487/1‑150 (MQ=255)
                      gcgcATCTTGAAGCGCATCTGCAAACCCCGAACATGAAGGCGTATAGCGAATCCGTAAAATGTGAAGTGATGGAGATGAATATCCGTATTCTGCAGCCAGGGATGTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCTGGCAATGTTCt                                                                                                                               >  1:1543174/1‑150 (MQ=255)
                      gcgcATCTAGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGACGCCGTAAAAGGTGACGTGCGGGAGAGGAATCTCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCt                                                                                                                               <  1:111353/150‑1 (MQ=255)
                        gcATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCttt                                                                                                                             <  2:1626491/150‑1 (MQ=255)
                        gcATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGAGGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCttt                                                                                                                             <  1:414247/150‑1 (MQ=255)
                         cATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTc                                                                                                                            <  2:1380176/150‑1 (MQ=255)
                         cATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTc                                                                                                                            >  2:1617692/1‑150 (MQ=255)
                           ccTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCt                                                                                                                          <  2:792868/149‑1 (MQ=255)
                            cTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCtt                                                                                                                         <  2:936880/150‑1 (MQ=255)
                              tGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTa                                                                                                                       <  1:1160897/150‑1 (MQ=255)
                               gAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTaa                                                                                                                      <  2:311935/150‑1 (MQ=255)
                                 aGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAAc                                                                                                                    >  1:1167962/1‑150 (MQ=255)
                                  gcgcATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAAcc                                                                                                                   >  2:1170268/1‑150 (MQ=255)
                                  gcgcATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAAcc                                                                                                                   <  1:568869/150‑1 (MQ=255)
                                    gcATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTc                                                                                                                 >  1:1649265/1‑150 (MQ=255)
                                      aTCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCaa                                                                                                               >  2:1527661/1‑150 (MQ=255)
                                       tCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAAt                                                                                                              >  1:1342484/1‑150 (MQ=255)
                                           cAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGCCCGGGCAATGTTATGTCCTTTAAACCTCAATCTcc                                                                                                          >  2:701740/1‑150 (MQ=255)
                                             aaCCCCTCATATTAAGGCTTATATCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGc                                                                                                        <  2:1322613/150‑1 (MQ=255)
                                             aaCCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGc                                                                                                        <  1:233038/150‑1 (MQ=255)
                                                 ccGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATg                                                                                                    >  2:1036568/1‑150 (MQ=255)
                                                  cGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGt                                                                                                   >  1:639117/1‑150 (MQ=255)
                                                   gCACATGAAGGAGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGACTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTc                                                                                                  <  1:1170268/150‑1 (MQ=255)
                                                       aTGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATTTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCt                                                                                              <  2:1691073/150‑1 (MQ=255)
                                                       aTGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCt                                                                                              <  2:1404027/150‑1 (MQ=255)
                                                          aaGGCGGATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTtc                                                                                           <  2:134957/150‑1 (MQ=255)
                                                            ggCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTtctc                                                                                         <  1:356736/150‑1 (MQ=255)
                                                             gCGTATAGCGAGGCCGTAAAAGGTGACGTGCTGGAGATGAATCTCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTctct                                                                                        <  1:1457268/150‑1 (MQ=255)
                                                              cGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCtt                                                                                       >  2:750431/1‑150 (MQ=255)
                                                               gTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCGTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTg                                                                                      <  1:327541/150‑1 (MQ=255)
                                                               gTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTg                                                                                      >  1:1005458/1‑150 (MQ=255)
                                                               gTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTg                                                                                      <  2:967065/150‑1 (MQ=255)
                                                                    gCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAAcc                                                                                 <  1:1534171/150‑1 (MQ=255)
                                                                          ccGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTgc                                                                           <  1:487282/150‑1 (MQ=255)
                                                                           cGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTgct                                                                          >  1:261715/1‑149 (MQ=255)
                                                                             tAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTgcggc                                                                        <  1:943719/150‑1 (MQ=255)
                                                                              aaaaGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTgcggcg                                                                       >  1:208394/1‑150 (MQ=255)
                                                                               aaaGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGa                                                                      >  1:1298884/1‑150 (MQ=255)
                                                                                 aGGTGACGTGCTGGAGATGAATATCCGTATGCTGCAGCCAGGGATTTAAACCAGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTGCTTTCCTTTTATCCTCAATCTCCGCCTTGTCGCCTTTCTCTTGCAACCAGTGGCGGCGATc                                                                    <  1:35066/150‑1 (MQ=255)
                                                                                   gTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCtt                                                                  >  2:1243222/1‑150 (MQ=255)
                                                                                   gTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCtt                                                                  >  1:522971/1‑150 (MQ=255)
                                                                                           cTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGt                                                          >  2:1468469/1‑150 (MQ=255)
                                                                                             ggAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGttt                                                        >  1:597918/1‑150 (MQ=255)
                                                                                             ggAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGttt                                                        >  2:1671395/1‑150 (MQ=255)
                                                                                             ggAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCATGCCTTGTTTGCCCGGCCATCCTGAGCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGttt                                                        <  1:1542231/150‑1 (MQ=255)
                                                                                               agaTGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCAGGCAATCCGGACAGGCCAATGTTCTTTCCTTTAAAACTCAATCTCCGCAATGTCGACTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCCTttct                                                      >  1:823175/1‑150 (MQ=255)
                                                                                                 aTGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGCTCTTCCGAGCGTttcttc                                                    >  1:1674541/1‑150 (MQ=255)
                                                                                                  tGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCg                                                   <  2:110800/150‑1 (MQ=255)
                                                                                                  tGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCg                                                   <  2:440960/150‑1 (MQ=255)
                                                                                                  tGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCg                                                   >  2:1573491/1‑150 (MQ=255)
                                                                                                  tGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCg                                                   >  1:1647465/1‑150 (MQ=255)
                                                                                                   gAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGAGGCGATCTTCCGAGCGTTTCTTCGc                                                  >  2:870768/1‑150 (MQ=255)
                                                                                                    aaTATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGcc                                                 <  1:1124579/150‑1 (MQ=255)
                                                                                                    aaTATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGcc                                                 <  1:1400628/150‑1 (MQ=255)
                                                                                                      tatCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCAGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCcag                                               <  1:708254/150‑1 (MQ=255)
                                                                                                       atCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCcagc                                              >  2:1027284/1‑150 (MQ=255)
                                                                                                         ccGTATTCTGCAGCCAGGGATTTAATCATGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCAATGTCGCCTTTCTATTGCAACCAGTTTCGGAGATCTTCCTAGCGTTTCTTCGCcagcaa                                            >  1:316204/1‑149 (MQ=255)
                                                                                                           gTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCACCCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAgcggca                                          >  1:928328/1‑150 (MQ=255)
                                                                                                              ttCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCatat                                       <  1:1088671/150‑1 (MQ=255)
                                                                                                                      ccAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCatcatc                               <  1:1457601/150‑1 (MQ=255)
                                                                                                                       cAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATcg                              >  2:1562150/1‑150 (MQ=255)
                                                                                                                         gggATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATcgcg                            >  2:1640231/1‑150 (MQ=255)
                                                                                                                         gggATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATcgcg                            >  2:211790/1‑150 (MQ=255)
                                                                                                                         gggATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATcgcg                            <  1:1438570/150‑1 (MQ=255)
                                                                                                                            aTTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCa                         >  1:1013925/1‑150 (MQ=255)
                                                                                                                            aTTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCa                         >  2:566254/1‑150 (MQ=255)
                                                                                                                                aaTCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTAc                     >  2:1243908/1‑150 (MQ=255)
                                                                                                                                  tCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGc                   <  2:499213/150‑1 (MQ=255)
                                                                                                                                    cTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTg                 >  1:939380/1‑150 (MQ=255)
                                                                                                                                     tGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGa                >  1:1551728/1‑150 (MQ=255)
                                                                                                                                      gCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGAt               >  2:682/1‑150 (MQ=255)
                                                                                                                                        cgtGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGCTCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCg             <  1:76129/148‑1 (MQ=255)
                                                                                                                                           gTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCt          <  2:261715/150‑1 (MQ=255)
                                                                                                                                           cattGCCCGGTCAGGATGGCCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCt          >  2:874135/3‑150 (MQ=255)
                                                                                                                                            attGCCCGGTCAGGATGGCCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCCTCGCGTCAGTCCGCTGATCGTCtt         >  2:905239/2‑150 (MQ=255)
                                                                                                                                              tGCCCGGTCAGGATGGCCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTACTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTATCATCGCCAGCAGCATATCCAACATCGCGTCAGTACGCTGATCGTCTtca       >  1:608835/1‑150 (MQ=255)
                                                                                                                                              tGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTtca       <  2:1023010/150‑1 (MQ=255)
                                                                                                                                               gCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTtcat      >  2:1138577/1‑150 (MQ=255)
                                                                                                                                               gCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTtcat      >  1:1618171/1‑150 (MQ=255)
                                                                                                                                               gCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCCtcaa      >  2:125822/1‑149 (MQ=255)
                                                                                                                                                cccGGCCATCCTGACCGGGCAATGTTCTTTGCTTTAACCCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATCTCCATCATCGCGTCAGTACGCTGATCGTCTtcata     >  2:835503/1‑149 (MQ=255)
                                                                                                                                                 ccGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTtcatca    >  2:492935/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GATTGAGCAGTGGGAAAGCATCGCGCATCTTGAAGCGCATCTGCAAACCCCGCACATGAAGGCGTATAGCGAAGCCGTAAAAGGTGACGTGCTGGAGATGAATATCCGTATTCTGCAGCCAGGGATTTAATCCTGCCTTGTTTGCCCGGCCATCCTGACCGGGCAATGTTCTTTCCTTTAAACCTCAATCTCCGCCATGTCGCCTTTCTCTTGCAACCAGTTGCGGCGATCTTCCGAGCGTTTCTTCGCCAGCAGCATATCCATCATCGCGTCAGTACGCTGATCGTCTTCATCATC  >  NC_000913/3173327‑3173623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: