BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,180,690	G→A	5.7%	W90* (TGG→TGA)	ygiC →	ATP‑Grasp family ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,180,690	0	G	A	5.7%	443.3 / 8.5	140	W90* (TGG→TGA)	ygiC	ATP‑Grasp family ATPase
Reads supporting (aligned to +/- strand): ref base G (60/71); new base A (4/4); total (64/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01

Only 100 of 144 total aligned reads displayed.

ACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGGCTGGAAGATCAGCTTAA  >  NC_000913/3180541‑3180839

aCCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATgg                                                                                                                                                       <  1:1364521/150‑1 (MQ=255)

aCCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTAACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATgg                                                                                                                                                       >  1:1505733/1‑150 (MQ=255)

 cccTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGc                                                                                                                                                      >  2:712098/1‑150 (MQ=255)

 cccTCGCCCAGGGTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGc                                                                                                                                                      <  2:1108911/150‑1 (MQ=255)

      gCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACg                                                                                                                                                 <  1:1324488/150‑1 (MQ=255)

       cccAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGc                                                                                                                                                <  2:238359/150‑1 (MQ=255)

       cccAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGc                                                                                                                                                >  1:154675/1‑150 (MQ=255)

          aGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGTCAGCCATGACTGGCGCAca                                                                                                                                             <  2:1272637/150‑2 (MQ=255)

          aGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGAAcc                                                                                                                                             >  1:1040050/1‑150 (MQ=255)

           ggTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGTCAGCCATGACTGGCGCAcaa                                                                                                                                            <  1:441055/150‑3 (MQ=255)

            gTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGTCAGCCATGACTGGCGCAcaaa                                                                                                                                           <  1:1627184/150‑4 (MQ=255)

             ttGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGc                                                                                                                                          <  1:813721/150‑1 (MQ=255)

               gAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGCTGACCAAATTCCGCATTCCAAAACACACATGGAGTTTTGTGCGCCAGTCCTGGCTGACGCACCAGCCa                                                                                                                                        >  1:232476/1‑150 (MQ=255)

                 aaaGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGGTGCGTCAGCCAtg                                                                                                                                      <  2:1583995/150‑2 (MQ=255)

                   aGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGGTGCGTCAGCCAtgac                                                                                                                                    <  2:1566463/150‑4 (MQ=255)

                   aGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGGTGCGTCAGCCAtgac                                                                                                                                    <  2:12029/150‑4 (MQ=255)

                   aGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGc                                                                                                                                    <  1:1695381/150‑1 (MQ=255)

                   aGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGc                                                                                                                                    <  1:1688785/150‑1 (MQ=255)

                     cTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCtt                                                                                                                                  <  1:342144/150‑1 (MQ=255)

                      tGGAAGAAGTCACCGCCGAACTGCACCAGATTTGCCTGAACGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTACGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCttt                                                                                                                                 <  1:941836/150‑1 (MQ=255)

                       ggAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGGTGCGTCAGCCAtgactggc                                                                                                                                <  2:783506/150‑8 (MQ=255)

                       ggAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTa                                                                                                                                >  1:683085/1‑150 (MQ=255)

                       ggAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGCAGTTTTGTGCGCCAGTCATGGATTACGCACCAGCCATCGCTTTa                                                                                                                                >  1:1125780/1‑150 (MQ=255)

                         aagaagTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTAtt                                                                                                                              <  2:1131972/150‑1 (MQ=255)

                         aagaagTCACCGCCGAACACCACCCGATGAGCCTTAAAGTGGTGGAAAAAGTGATCGCCAGCGACGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGAGTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTAtt                                                                                                                              <  1:864579/150‑1 (MQ=255)

                           gaagGCACCGCCGAAATGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTTATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTcg                                                                                                                            <  2:833072/150‑1 (MQ=255)

                            aagTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTcgc                                                                                                                           >  2:1446260/1‑150 (MQ=255)

                             agTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTcgcg                                                                                                                          <  1:793040/150‑1 (MQ=255)

                               tCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTc                                                                                                                        <  1:742993/150‑1 (MQ=255)

                                cACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCt                                                                                                                       >  2:85966/1‑150 (MQ=255)

                                cACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCt                                                                                                                       >  2:1151097/1‑150 (MQ=255)

                                  ccgccgAACTGCACCATATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAACCATCGCTTTATTCGCGTCTTg                                                                                                                     <  2:596239/150‑1 (MQ=255)

                                  ccgccgAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTg                                                                                                                     <  2:411449/150‑1 (MQ=255)

                                  ccgccgAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGGTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTg                                                                                                                     <  2:325101/150‑1 (MQ=255)

                                   cgccgAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGTTCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGa                                                                                                                    >  2:501654/1‑150 (MQ=255)

                                   cgccgAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGa                                                                                                                    >  2:1651773/1‑150 (MQ=255)

                                    gccgAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGACTGACGCACCAGCCATCGCtt                                                                                                                                  >  2:1698737/1‑135 (MQ=255)

                                     ccgAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATc                                                                                                                  <  2:260690/150‑1 (MQ=255)

                                     ccgAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATc                                                                                                                  >  1:1010320/1‑150 (MQ=255)

                                     ccgAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATc                                                                                                                  <  1:679400/150‑1 (MQ=255)

                                        taCTGCACCAGATGTGCAGGAAAGTGTTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTgg                                                                                                               <  1:205325/149‑1 (MQ=255)

                                         aCTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGc                                                                                                              <  2:897479/150‑1 (MQ=255)

                                          cTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCg                                                                                                             <  2:976714/150‑1 (MQ=255)

                                           tGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGt                                                                                                            >  1:837131/1‑150 (MQ=255)

                                           tGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGt                                                                                                            >  2:421062/1‑150 (MQ=255)

                                            gCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTg                                                                                                           <  2:305259/150‑1 (MQ=255)

                                             cACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTTATCTGGCGTgg                                                                                                          <  1:981617/150‑1 (MQ=255)

                                              aCCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTggg                                                                                                         >  1:1311907/1‑150 (MQ=255)

                                              aCCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTggg                                                                                                         <  1:933931/150‑1 (MQ=255)

                                                 aGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGCCGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATg                                                                                                      >  2:892061/1‑150 (MQ=255)

                                                 aGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATg                                                                                                      <  1:547901/150‑1 (MQ=255)

                                                  gATGTGTCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATgg                                                                                                     >  1:47726/1‑150 (MQ=255)

                                                      tgCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACt                                                                                                 >  2:531141/1‑150 (MQ=255)

                                                        ccTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTgg                                                                                               <  1:1708303/150‑1 (MQ=255)

                                                         cTGATAGTGGTGGAAAATGTGATCGCCAGCGATGAGCTGATGACCAAATTGAGCATTCCAAAACACACCTGGAGGTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATGGATTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGt                                                                                              <  1:264742/150‑1 (MQ=255)

                                                         cTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGt                                                                                              >  2:987270/1‑150 (MQ=255)

                                                           gAAAGTGGTGGAAAAAGTGAACGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGa                                                                                            <  2:1190738/150‑1 (MQ=255)

                                                            aaaGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGaa                                                                                           >  1:343697/1‑150 (MQ=255)

                                                                   tggAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGAGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAAc                                                                                    >  1:194634/1‑150 (MQ=255)

                                                                   gggAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAAc                                                                                    <  2:1578808/149‑1 (MQ=255)

                                                                    ggAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACt                                                                                   <  2:654383/150‑1 (MQ=255)

                                                                      aaaaaGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTc                                                                                 <  2:906081/150‑1 (MQ=255)

                                                                       aaaaGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTTATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCt                                                                                <  2:850586/150‑1 (MQ=255)

                                                                         aaGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTgg                                                                              <  2:1671284/150‑1 (MQ=255)

                                                                            tGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGaaa                                                                           >  1:923605/1‑150 (MQ=255)

                                                                             gATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGaaaa                                                                          <  2:773569/150‑1 (MQ=255)

                                                                              aTCGCCAGCGATGAGGTGATGACCAAATTCCGCATTCCAAAACACACCTGTAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGGGGGAGGGCACTGGTGAACCTAAACTTCTGGAAAAt                                                                         <  2:342566/150‑1 (MQ=255)

                                                                               tCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATa                                                                        <  1:1637808/150‑1 (MQ=255)

                                                                                  ccAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGGTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACg                                                                     <  1:1155798/150‑1 (MQ=255)

                                                                                    aGCGATGAGCTTATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTTTGCGCCATTCATGGCTGACTCACCAGCCATCGCTTTATTCTCGTCTTGATCTGGCGTGGGATGTCACTGGTGAACCTAAACTTCTGGAAAATAACGcc                                                                   <  1:1613359/150‑1 (MQ=255)

                                                                                            gCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGcc                                                           <  2:187030/150‑1 (MQ=255)

                                                                                                  gaCCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTc                                                     >  1:1599092/1‑150 (MQ=255)

                                                                                                  gaCCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGAGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTc                                                     >  1:1605461/1‑150 (MQ=255)

                                                                                                   aacAACTTCCGGATTCCAAAACACACCTGGAGTTTTGTGCGGCCTTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCa                                                    <  2:1604622/148‑1 (MQ=255)

                                                                                                    ccAAATTCCGCATTCCAACACACAGCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGGGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCAc                                                   <  1:604626/150‑1 (MQ=255)

                                                                                                    ccAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCAc                                                   >  2:1469220/1‑150 (MQ=255)

                                                                                                    ccAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCAc                                                   <  1:501654/150‑1 (MQ=255)

                                                                                                      aaaTTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACta                                                 >  1:629368/1‑150 (MQ=255)

                                                                                                       aaTTCCGGATTCCAAAACACACCTGGAGTTTTGTGCGACAGTCAGGTCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGTCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACtat                                                <  1:981263/150‑1 (MQ=255)

                                                                                                       aaTTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACtat                                                >  2:1442594/1‑150 (MQ=255)

                                                                                                        aTTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACtata                                               <  1:252170/150‑1 (MQ=255)

                                                                                                        aTTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACtata                                               <  1:743090/150‑1 (MQ=255)

                                                                                                           ccGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGa                                            >  1:863612/1‑150 (MQ=255)

                                                                                                              cATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAggc                                         >  1:510650/1‑150 (MQ=255)

                                                                                                              cATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAggc                                         >  2:1353767/1‑150 (MQ=255)

                                                                                                               aTTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAggcg                                        >  1:1267664/1‑150 (MQ=255)

                                                                                                                         acacCTGGAGTTGTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTc                              <  2:908810/150‑1 (MQ=255)

                                                                                                                            ccTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGGCGTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCGTTCAGt                           >  2:1040383/1‑150 (MQ=255)

                                                                                                                                  gTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATct                     >  1:1727090/1‑150 (MQ=255)

                                                                                                                                  gTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATct                     >  1:1647728/1‑150 (MQ=255)

                                                                                                                                     tggtgCGTCAGCCATGACTGGCGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATctggc                  >  2:413913/3‑150 (MQ=255)

                                                                                                                                      tgtgCGCCAGTCAGGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATctggct                 <  2:193374/150‑1 (MQ=255)

                                                                                                                                      ggtgCGTCAGCCATGACTGGCGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATctggct                 >  1:1279397/2‑150 (MQ=255)

                                                                                                                                       gtgCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATctggctg                >  2:742032/1‑150 (MQ=255)

                                                                                                                                           taaagcgaTGGCTGGTGCGTCAGCCATCGCTTTTTTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGGCTGGAAg            >  1:113565/8‑150 (MQ=255)

                                                                                                                                            ccAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGGCTGGAAGa           >  1:616668/1‑150 (MQ=255)

                                                                                                                                                cgaTGGCTGGTGCGTCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGGCTGGAAGATCAg       >  1:757078/3‑150 (MQ=255)

                                                                                                                                                  aTGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGGCTGGAAGATCAGCt     >  1:845087/1‑150 (MQ=255)

                                                                                                                                                    ggCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGGCTGGAAGATCAGCTTa   >  1:399884/1‑150 (MQ=255)

                                                                                                                                                     gCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGGCTGGAAGATCAGCTTaa  >  1:146471/1‑150 (MQ=255)

ACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGGCTGGAAGATCAGCTTAA  >  NC_000913/3180541‑3180839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`