Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,671 C→A 6.3% A70D (GCC→GAC)  ttdA → L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,6710CA 6.3% 332.8 / 7.4 111A70D (GCC→GAC) ttdAL‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (42/60);  new base A (4/4);  total (47/64)
Fisher's exact test for biased strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

CTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACG  >  NC_000913/3206522‑3206820
                                                                                                                                                     |                                                                                                                                                     
cTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGAAGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgc                                                                                                                                                       >  1:1093680/1‑150 (MQ=255)
 tttACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcc                                                                                                                                                      <  2:1114650/150‑1 (MQ=255)
 tttACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcc                                                                                                                                                      <  2:301079/150‑1 (MQ=255)
   tACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcctg                                                                                                                                                    <  1:597294/150‑1 (MQ=255)
     ccGCCATGATTTCTACCCGAACGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTc                                                                                                                                                  <  1:1220243/150‑1 (MQ=255)
       gCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAg                                                                                                                                                <  1:1344607/150‑1 (MQ=255)
       gCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAg                                                                                                                                                >  2:1644189/1‑150 (MQ=255)
       gCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGAGTGACCTGAATCGTCCTGCCTGTCAg                                                                                                                                                <  2:1456725/150‑1 (MQ=255)
        ccATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAgg                                                                                                                                               <  1:1345324/150‑1 (MQ=255)
          aTGATTTCTACCCGAATGCCTGATGACGTGGTTGATAAAATAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGACAGGCAGGac                                                                                                                                             <  1:125849/150‑1 (MQ=255)
           tGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGaca                                                                                                                                            >  1:1539797/1‑150 (MQ=255)
               ttCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgg                                                                                                                                        >  1:583351/1‑150 (MQ=255)
                 cTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                                                                                                      <  2:1159944/150‑1 (MQ=255)
                 cTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGACAGGCAGGacgattcag                                                                                                                                      <  1:172883/150‑8 (MQ=255)
                 cTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGACAGGCAGGacgattcag                                                                                                                                      <  1:1193569/150‑8 (MQ=255)
                    cccGAATGCCTGATGACGGGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGgaga                                                                                                                                   <  2:122852/150‑1 (MQ=255)
                     ccGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAt                                                                                                                                  <  2:563709/150‑1 (MQ=255)
                      ctaaTGCCTGATGAAGGGGTGGATAAACTACACCAGCTAAAGGATGCCGAAACGACGTCGATGGTGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAtt                                                                                                                                 <  2:137127/148‑1 (MQ=255)
                       gAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTa                                                                                                                                <  2:1664409/150‑1 (MQ=255)
                            ccTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGttc                                                                                                                           >  2:743933/1‑150 (MQ=255)
                              tgatgaCGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGttctt                                                                                                                         <  1:1333241/150‑1 (MQ=255)
                                atgaCGTGGTGGATAAACAAAAACAGCTAAAGGATGCAGCAACGTCGGCGATGGTGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCg                                                                                                                       <  1:119247/150‑1 (MQ=255)
                                 tgaCGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGt                                                                                                                      <  1:1031901/150‑1 (MQ=255)
                                 tgaCGTCGTGGCTAAACTAAAAGAGCTAAAGGATGCAGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGt                                                                                                                      <  2:462305/150‑1 (MQ=255)
                                  gaCGTGGTGGATTAACTAACACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGtt                                                                                                                     <  2:337376/150‑1 (MQ=255)
                                  gaCGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGATCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGtt                                                                                                                     <  1:403742/150‑1 (MQ=255)
                                   aCGTGGTGGATAAACTAAAACAGCTAAAGGATGTCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTa                                                                                                                    <  2:973957/150‑1 (MQ=255)
                                      ttgtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGGCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg                                                                                                                 <  1:224514/148‑1 (MQ=255)
                                       ggtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGt                                                                                                                >  2:488563/1‑150 (MQ=255)
                                       ggtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGACAGGCAGGacgat                                                                                                                                          >  2:902376/1‑121 (MQ=255)
                                       ggtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGACAGGCAGGacgat                                                                                                                                          <  1:902376/124‑4 (MQ=255)
                                        ttggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTc                                                                                                               <  1:1558107/149‑1 (MQ=255)
                                        gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCTATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTc                                                                                                               <  1:1064592/150‑1 (MQ=255)
                                        gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGAGTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTc                                                                                                               <  1:1065980/150‑1 (MQ=255)
                                        gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAGGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTc                                                                                                               <  2:910139/150‑1 (MQ=255)
                                         tggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCg                                                                                                              <  2:293733/150‑1 (MQ=255)
                                          ggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCgg                                                                                                             <  2:789322/150‑1 (MQ=255)
                                             tAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc                                                                                                          <  2:1622444/150‑1 (MQ=255)
                                              aaaCTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACAATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTcc                                                                                                         >  1:482092/1‑150 (MQ=255)
                                                aCTAAAACATCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCg                                                                                                       <  1:331727/150‑1 (MQ=255)
                                                  tAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCt                                                                                                     <  1:845677/150‑1 (MQ=255)
                                                   aaaaCAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCtt                                                                                                    >  2:8637/1‑150 (MQ=255)
                                                   aaaaCAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTACTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCCCtt                                                                                                    >  1:915147/1‑150 (MQ=255)
                                                    aaaCAGCTAAAGGATGCCGAAACGTCGTCGATGGTGAAAATTATCAACCATACGATATTCTACAACATGCAAAAAGCGATTGACCAGAATAGTCCTGACAGTCAGGACACCGTGGAGCATATGTTATTCGTTAAAGTCAGTTCCCGCTTc                                                                                                   >  1:330328/1‑150 (MQ=255)
                                                      aCAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACGCCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTccc                                                                                                 <  1:374697/150‑1 (MQ=255)
                                                        aGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCAc                                                                                               <  2:579510/150‑1 (MQ=255)
                                                           aaaaGGATTCCGAAACGTCGTCGATGGGTAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGCCCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGc                                                                                            <  2:76337/149‑1 (MQ=255)
                                                            aaaGGAAGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGAATGACCTGACTCGTCCTGCCTGTCAGGACACCGGGGACATTAAGATCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCt                                                                                           >  1:128016/1‑150 (MQ=255)
                                                                 aTGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCg                                                                                      <  2:300544/150‑1 (MQ=255)
                                                                     cGAAACGTCGTCGATGGGGAATATTATCTACCATACGATGGTCCACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCt                                                                                  <  1:935839/150‑1 (MQ=255)
                                                                      gAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTg                                                                                 <  1:410352/150‑1 (MQ=255)
                                                                       aatcgtcgtcgtTGGGGAAAATTAGTTACCATACGATGTTCGACATCTTGCATAAAGCGATTGACCTGAATCGTGCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCTCTGGTTGGCGAGCTGc                                                                                <  2:1420815/147‑1 (MQ=255)
                                                                        aaCGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCa                                                                               <  1:1239976/150‑1 (MQ=255)
                                                                           gtcgtcGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCaaac                                                                            >  2:415869/1‑149 (MQ=255)
                                                                                   tGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTCACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCa                                                                    >  2:1039935/1‑150 (MQ=255)
                                                                                    ggggAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCaa                                                                   <  2:1311212/150‑1 (MQ=255)
                                                                                    ggggAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCaa                                                                   >  2:1022262/1‑150 (MQ=255)
                                                                                       gAAAATTATCTATCATACGATGTTCTACAACAGGCAAATAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATAATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACa                                                                <  2:1131172/150‑1 (MQ=255)
                                                                                          aaTTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGc                                                             <  1:429247/150‑1 (MQ=255)
                                                                                           aTTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGTCCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGcc                                                            <  1:1381979/150‑1 (MQ=255)
                                                                                              aTCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTg                                                         >  1:1389239/1‑150 (MQ=255)
                                                                                                cTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGa                                                       >  1:413783/1‑150 (MQ=255)
                                                                                                    cATACGATGTTCGACGACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAagag                                                   <  1:538913/150‑1 (MQ=255)
                                                                                                    cATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGGCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAagag                                                   <  2:970268/150‑1 (MQ=255)
                                                                                                    cATACGATGTGCGACACCATGCAAAAAGCGATTGACCTCAAGCGTCCTGCCTGTCAGGACACCGGGGAGAATATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCCCTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAagag                                                   <  1:288946/150‑1 (MQ=255)
                                                                                                     aTACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGACCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGATACCCAATGATTGCCGAGCTGCAAAGCATACACAAACAAGCCGTGGAATAgg                                                  >  2:943739/1‑150 (MQ=255)
                                                                                                     aTACGATGTTCGACAACAAGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAgg                                                  <  1:1366602/150‑1 (MQ=255)
                                                                                                      tACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGc                                                 <  1:630498/150‑1 (MQ=255)
                                                                                                      tACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGc                                                 <  1:488563/150‑1 (MQ=255)
                                                                                                       aCGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCa                                                >  2:291256/1‑150 (MQ=255)
                                                                                                        cGATGTTCGCCAACATGCAAAAAGCGATTTACCTGAATAGTCCTGACTGTCAGGACACAGGTGAGATTATTTTCTTAGTTATAGTCGTTTCCCGATTACGACTGCTTGGCGAGCTTCAAAGAATACTCAAACAAGCCGTGGaatattaaa                                               >  2:174784/1‑143 (MQ=255)
                                                                                                        cGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCac                                               >  2:1029573/1‑149 (MQ=255)
                                                                                                          aTGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAAcc                                             >  1:14965/1‑150 (MQ=255)
                                                                                                               cGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCaa                                        <  2:702989/150‑1 (MQ=255)
                                                                                                                 acaacaTGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAg                                      >  2:972831/1‑150 (MQ=255)
                                                                                                                  caacaTGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAgc                                     <  2:592085/150‑1 (MQ=255)
                                                                                                                   aacaTGCAAAACGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAgcg                                    <  2:851276/150‑1 (MQ=255)
                                                                                                                   aacaTGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAgcg                                    <  2:811587/150‑1 (MQ=255)
                                                                                                                   aacaTGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAgcg                                    <  1:134223/150‑1 (MQ=255)
                                                                                                                     caTGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGcc                                  >  1:1325509/1‑150 (MQ=255)
                                                                                                                      aTGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCa                                 >  1:1140150/1‑150 (MQ=255)
                                                                                                                      aTGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCa                                 <  1:983051/150‑1 (MQ=255)
                                                                                                                          aaaaaGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTAc                             >  1:1474594/1‑150 (MQ=255)
                                                                                                                          aaaaaGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATAATCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTAc                             >  1:540876/1‑150 (MQ=255)
                                                                                                                                cGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTcaca                       >  1:765599/1‑150 (MQ=255)
                                                                                                                                   ttGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATg                    <  2:483762/150‑1 (MQ=255)
                                                                                                                                     gACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCg                  >  1:309162/1‑150 (MQ=255)
                                                                                                                                     gACATGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAAAAAGCCGTGGAAGAGGCACACGTCAAAGCGCCACTACGTCACAATGCg                  >  2:1495055/1‑150 (MQ=255)
                                                                                                                                      aCCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAATATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCgg                 <  1:96961/150‑1 (MQ=255)
                                                                                                                                       ccTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGt                >  1:159203/1‑150 (MQ=255)
                                                                                                                                         tGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAg              >  1:364413/1‑150 (MQ=255)
                                                                                                                                         cccggtgTCCTGACAGGCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCTCAATGCGGTAg              >  1:929213/7‑150 (MQ=255)
                                                                                                                                               gTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAAttt        >  2:1650863/1‑150 (MQ=255)
                                                                                                                                               gTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAAttt        >  1:763151/1‑150 (MQ=255)
                                                                                                                                                 cctgcctgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAAttttt      >  1:1008056/1‑150 (MQ=255)
                                                                                                                                                  ctgcctgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTg     <  1:1128325/150‑1 (MQ=255)
                                                                                                                                                   tgcctgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGa    >  2:475733/1‑150 (MQ=255)
                                                                                                                                                    gcctgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCACCCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGAc   >  1:1706392/1‑150 (MQ=255)
                                                                                                                                                    gcctgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGAc   <  1:1039935/150‑1 (MQ=255)
                                                                                                                                                     cctgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCCCAATGCGGTAGAAATTTTTGACg  >  1:1368119/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACG  >  NC_000913/3206522‑3206820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: