Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,673 T→A 5.9% C71S (TGT→AGT) ‡ ttdA → L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,6730TA 5.9% 327.4 / 7.1 111C71S (TGT→AGT) ‡ttdAL‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (43/60);  new base A (3/4);  total (47/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-01

Only 100 of 122 total aligned reads displayed.

TACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGAA  >  NC_000913/3206525‑3206822
                                                                                                                                                    |                                                                                                                                                     
tACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcctg                                                                                                                                                      <  1:597294/150‑1 (MQ=255)
    gCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAg                                                                                                                                                  >  2:1644189/1‑150 (MQ=255)
    gCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAg                                                                                                                                                  <  1:1344607/150‑1 (MQ=255)
    gCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGAGTGACCTGAATCGTCCTGCCTGTCAg                                                                                                                                                  <  2:1456725/150‑1 (MQ=255)
     ccATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAgg                                                                                                                                                 <  1:1345324/150‑1 (MQ=255)
       aTGATTTCTACCCGAATGCCTGATGACGTGGTTGATAAAATAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGACAGGCAGGac                                                                                                                                               <  1:125849/150‑1 (MQ=255)
        tGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGaca                                                                                                                                              >  1:1539797/1‑150 (MQ=255)
              cTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                                                                                                        <  2:1159944/150‑1 (MQ=255)
              cTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGACAGGCAGGacgattcag                                                                                                                                        <  1:172883/150‑8 (MQ=255)
              cTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGACAGGCAGGacgattcag                                                                                                                                        <  1:1193569/150‑8 (MQ=255)
                aCCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGgag                                                                                                                                      >  1:811587/1‑150 (MQ=255)
                 cccGAATGCCTGATGACGGGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGgaga                                                                                                                                     <  2:122852/150‑1 (MQ=255)
                  ccGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAt                                                                                                                                    <  2:563709/150‑1 (MQ=255)
                   ctaaTGCCTGATGAAGGGGTGGATAAACTACACCAGCTAAAGGATGCCGAAACGACGTCGATGGTGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAtt                                                                                                                                   <  2:137127/148‑1 (MQ=255)
                   cGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAtt                                                                                                                                   <  2:477431/150‑1 (MQ=255)
                    gAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTa                                                                                                                                  <  2:1664409/150‑1 (MQ=255)
                         ccTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGttc                                                                                                                             >  2:743933/1‑150 (MQ=255)
                           tgatgaCGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGttctt                                                                                                                           <  1:1333241/150‑1 (MQ=255)
                           tgatgaCGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGGCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGttctt                                                                                                                           <  1:1206717/150‑1 (MQ=255)
                             atgaCGTGGTGGATAAACAAAAACAGCTAAAGGATGCAGCAACGTCGGCGATGGTGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCg                                                                                                                         <  1:119247/150‑1 (MQ=255)
                              tgaCGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGt                                                                                                                        <  1:1031901/150‑1 (MQ=255)
                              tgaCGTCGTGGCTAAACTAAAAGAGCTAAAGGATGCAGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGt                                                                                                                        <  2:462305/150‑1 (MQ=255)
                               gaCGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGATCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGtt                                                                                                                       <  1:403742/150‑1 (MQ=255)
                                aCGTGGTGGATAAACTAAAACAGCTAAAGGATGTCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTa                                                                                                                      <  2:973957/150‑1 (MQ=255)
                                   ttgtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGGCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg                                                                                                                   <  1:224514/148‑1 (MQ=255)
                                    ggtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGt                                                                                                                  >  2:488563/1‑150 (MQ=255)
                                    ggtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGt                                                                                                                  >  2:630498/1‑150 (MQ=255)
                                    ggtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGACAGGCAGGacgat                                                                                                                                            <  1:902376/124‑4 (MQ=255)
                                    ggtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGACAGGCAGGacgat                                                                                                                                            >  2:902376/1‑121 (MQ=255)
                                     ttggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTc                                                                                                                 <  1:1558107/149‑1 (MQ=255)
                                     gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCTATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTc                                                                                                                 <  1:1064592/150‑1 (MQ=255)
                                     gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGAGTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTc                                                                                                                 <  1:1065980/150‑1 (MQ=255)
                                     gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAGGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTc                                                                                                                 <  2:910139/150‑1 (MQ=255)
                                      tggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCg                                                                                                                <  2:293733/150‑1 (MQ=255)
                                       ggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCgg                                                                                                               <  1:595131/150‑1 (MQ=255)
                                       ggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCgg                                                                                                               <  2:789322/150‑1 (MQ=255)
                                          tAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc                                                                                                            <  2:1622444/150‑1 (MQ=255)
                                           aaaCTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACAATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTcc                                                                                                           >  1:482092/1‑150 (MQ=255)
                                             aCTAAAACATCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCg                                                                                                         <  1:331727/150‑1 (MQ=255)
                                               tAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCt                                                                                                       <  1:845677/150‑1 (MQ=255)
                                                aaaaCAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCtt                                                                                                      >  2:8637/1‑150 (MQ=255)
                                                aaaaCAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTACTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCCCtt                                                                                                      >  1:915147/1‑150 (MQ=255)
                                                 aaaCAGCTAAAGGATGCCGAAACGTCGTCGATGGTGAAAATTATCAACCATACGATATTCTACAACATGCAAAAAGCGATTGACCAGAATAGTCCTGACAGTCAGGACACCGTGGAGCATATGTTATTCGTTAAAGTCAGTTCCCGCTTc                                                                                                     >  1:330328/1‑150 (MQ=255)
                                                   aCAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACGCCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTccc                                                                                                   <  1:374697/150‑1 (MQ=255)
                                                     aGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCAc                                                                                                 <  2:579510/150‑1 (MQ=255)
                                                       cTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACAATACGATGTTCGACAACATGCAAAAAGCGATTTACCTTAATCGTACTGCCTGTCAGTAAACAGGGGATATTATGTTATTAGTTAAAGTCGGTTCCCTATTCCAACTg                                                                                               >  2:321637/1‑150 (MQ=255)
                                                        aaaaGGATTCCGAAACGTCGTCGATGGGTAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGCCCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGc                                                                                              <  2:76337/149‑1 (MQ=255)
                                                         aaaGGAAGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGAATGACCTGACTCGTCCTGCCTGTCAGGACACCGGGGACATTAAGATCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCt                                                                                             >  1:128016/1‑150 (MQ=255)
                                                              aTGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCg                                                                                        <  2:300544/150‑1 (MQ=255)
                                                                   gAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTg                                                                                   <  1:410352/150‑1 (MQ=255)
                                                                    aatcgtcgtcgtTGGGGAAAATTAGTTACCATACGATGTTCGACATCTTGCATAAAGCGATTGACCTGAATCGTGCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCTCTGGTTGGCGAGCTGc                                                                                  <  2:1420815/147‑1 (MQ=255)
                                                                     aaCGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCa                                                                                 <  1:1239976/150‑1 (MQ=255)
                                                                        gtcgtcGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCaaac                                                                              >  2:415869/1‑149 (MQ=255)
                                                                                 ggggAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCaa                                                                     <  2:1311212/150‑1 (MQ=255)
                                                                                 ggggAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCaa                                                                     >  2:1022262/1‑150 (MQ=255)
                                                                                   ggAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGCCACCGGGGAGATTATGTTCTTCGTTAACGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAAc                                                                   >  2:1089593/1‑150 (MQ=255)
                                                                                    gAAAATTATCTATCATACGATGTTCTACAACAGGCAAATAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATAATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACa                                                                  <  2:1131172/150‑1 (MQ=255)
                                                                                    gAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGAGATTGACCTGAATCGTCCAGCCTGTCATTACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTACCCGCTTCCCACTGCTTGGCGACCTGCAAAGCATACTCAAACa                                                                  >  1:938141/1‑150 (MQ=255)
                                                                                       aaTTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGc                                                               <  1:429247/150‑1 (MQ=255)
                                                                                        aTTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGTCCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGcc                                                              <  1:1381979/150‑1 (MQ=255)
                                                                                           aTCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTg                                                           >  1:1389239/1‑150 (MQ=255)
                                                                                             cTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGa                                                         >  1:413783/1‑150 (MQ=255)
                                                                                                 cATACGATGTTCGACGACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAagag                                                     <  1:538913/150‑1 (MQ=255)
                                                                                                 cATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGGCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAagag                                                     <  2:970268/150‑1 (MQ=255)
                                                                                                 cATACGATGTGCGACACCATGCAAAAAGCGATTGACCTCAAGCGTCCTGCCTGTCAGGACACCGGGGAGAATATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCCCTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAagag                                                     <  1:288946/150‑1 (MQ=255)
                                                                                                  aTACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGACCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGATACCCAATGATTGCCGAGCTGCAAAGCATACACAAACAAGCCGTGGAATAgg                                                    >  2:943739/1‑150 (MQ=255)
                                                                                                  aTACGATGTTCGACAACAAGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAgg                                                    <  1:1366602/150‑1 (MQ=255)
                                                                                                   tACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGc                                                   <  1:488563/150‑1 (MQ=255)
                                                                                                   tACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGc                                                   <  1:630498/150‑1 (MQ=255)
                                                                                                    aCGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCa                                                  >  2:291256/1‑150 (MQ=255)
                                                                                                     cGATGTTCGCCAACATGCAAAAAGCGATTTACCTGAATAGTCCTGACTGTCAGGACACAGGTGAGATTATTTTCTTAGTTATAGTCGTTTCCCGATTACGACTGCTTGGCGAGCTTCAAAGAATACTCAAACAAGCCGTGGaatattaaa                                                 >  2:174784/1‑143 (MQ=255)
                                                                                                     cGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCac                                                 >  2:1029573/1‑149 (MQ=255)
                                                                                                       aTGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAAcc                                               >  1:14965/1‑150 (MQ=255)
                                                                                                              acaacaTGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAg                                        >  2:972831/1‑150 (MQ=255)
                                                                                                               caacaTGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAgc                                       <  2:592085/150‑1 (MQ=255)
                                                                                                                aacaTGCAAAACGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAgcg                                      <  2:851276/150‑1 (MQ=255)
                                                                                                                aacaTGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAgcg                                      <  1:134223/150‑1 (MQ=255)
                                                                                                                aacaTGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAgcg                                      <  2:811587/150‑1 (MQ=255)
                                                                                                                  caTGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGcc                                    >  1:1325509/1‑150 (MQ=255)
                                                                                                                   aTGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCa                                   <  1:983051/150‑1 (MQ=255)
                                                                                                                   aTGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCa                                   >  1:1140150/1‑150 (MQ=255)
                                                                                                                       aaaaaGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTAc                               >  1:1474594/1‑150 (MQ=255)
                                                                                                                       aaaaaGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATAATCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTAc                               >  1:540876/1‑150 (MQ=255)
                                                                                                                                  gACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCg                    >  1:309162/1‑150 (MQ=255)
                                                                                                                                  gACATGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAAAAAGCCGTGGAAGAGGCACACGTCAAAGCGCCACTACGTCACAATGCg                    >  2:1495055/1‑150 (MQ=255)
                                                                                                                                   aCCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAATATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCgg                   <  1:96961/150‑1 (MQ=255)
                                                                                                                                    ccTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGt                  >  1:159203/1‑150 (MQ=255)
                                                                                                                                      tGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAg                >  1:364413/1‑150 (MQ=255)
                                                                                                                                      cccggtgTCCTGACAGGCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCTCAATGCGGTAg                >  1:929213/7‑150 (MQ=255)
                                                                                                                                            gTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAAttt          >  2:1650863/1‑150 (MQ=255)
                                                                                                                                            gTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAAttt          >  1:763151/1‑150 (MQ=255)
                                                                                                                                              cctgcctgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAAttttt        >  1:1008056/1‑150 (MQ=255)
                                                                                                                                               ctgcctgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTg       <  1:1128325/150‑1 (MQ=255)
                                                                                                                                                tgcctgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGa      >  2:475733/1‑150 (MQ=255)
                                                                                                                                                 gcctgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCACCCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGAc     >  1:1706392/1‑150 (MQ=255)
                                                                                                                                                 gcctgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGAc     <  1:1039935/150‑1 (MQ=255)
                                                                                                                                                  cctgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCCCAATGCGGTAGAAATTTTTGACg    >  1:1368119/1‑150 (MQ=255)
                                                                                                                                                   ctgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGa   >  2:749247/1‑150 (MQ=255)
                                                                                                                                                    tgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGaa  >  1:482054/1‑150 (MQ=255)
                                                                                                                                                    tgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGaa  >  1:1239590/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGAA  >  NC_000913/3206525‑3206822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: