Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,675 T→G 6.1% C71W (TGT→TGG) ‡ ttdA → L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,6750TG 6.1% 344.2 / 8.9 122C71W (TGT→TGG) ‡ttdAL‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (52/61);  new base G (2/6);  total (55/67)
Fisher's exact test for biased strand distribution p-value = 2.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01

Only 100 of 123 total aligned reads displayed.

CCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGAAGT  >  NC_000913/3206527‑3206824
                                                                                                                                                    |                                                                                                                                                     
ccGCCATGATTTCTACCCGAACGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTc                                                                                                                                                      <  1:1220243/150‑1 (MQ=255)
  gCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAg                                                                                                                                                    >  2:1644189/1‑150 (MQ=255)
  gCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGAGTGACCTGAATCGTCCTGCCTGTCAg                                                                                                                                                    <  2:1456725/150‑1 (MQ=255)
   ccATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAgg                                                                                                                                                   <  1:1345324/150‑1 (MQ=255)
     aTGATTTCTACCCGAATGCCTGATGACGTGGTTGATAAAATAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGACAGGCAGGac                                                                                                                                                 <  1:125849/150‑1 (MQ=255)
      tGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGaca                                                                                                                                                >  1:1539797/1‑150 (MQ=255)
          ttCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgg                                                                                                                                            >  1:583351/1‑150 (MQ=255)
            cTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                                                                                                          <  2:1159944/150‑1 (MQ=255)
            cTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGACAGGCAGGacgattcag                                                                                                                                          <  1:172883/150‑8 (MQ=255)
              aCCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGgag                                                                                                                                        >  1:811587/1‑150 (MQ=255)
               cccGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGgaga                                                                                                                                       <  1:1081018/150‑1 (MQ=255)
               cccGAATGCCTGATGACGGGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGgaga                                                                                                                                       <  2:122852/150‑1 (MQ=255)
                ccGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAt                                                                                                                                      <  2:563709/150‑1 (MQ=255)
                 ctaaTGCCTGATGAAGGGGTGGATAAACTACACCAGCTAAAGGATGCCGAAACGACGTCGATGGTGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAtt                                                                                                                                     <  2:137127/148‑1 (MQ=255)
                 cGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAtt                                                                                                                                     <  2:477431/150‑1 (MQ=255)
                  gAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTa                                                                                                                                    <  2:1664409/150‑1 (MQ=255)
                       ccTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGttc                                                                                                                               >  2:743933/1‑150 (MQ=255)
                         tgatgaCGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGttctt                                                                                                                             <  1:1333241/150‑1 (MQ=255)
                         tgatgaCGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGGCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGttctt                                                                                                                             <  1:1206717/150‑1 (MQ=255)
                           atgaCGTGGTGGATAAACAAAAACAGCTAAAGGATGCAGCAACGTCGGCGATGGTGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCg                                                                                                                           <  1:119247/150‑1 (MQ=255)
                            tgaCGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGt                                                                                                                          <  1:1031901/150‑1 (MQ=255)
                            tgaCGTCGTGGCTAAACTAAAAGAGCTAAAGGATGCAGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGt                                                                                                                          <  2:462305/150‑1 (MQ=255)
                             gaCGTGGTGGATTAACTAACACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGtt                                                                                                                         <  2:337376/150‑1 (MQ=255)
                             gaCGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGATCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGtt                                                                                                                         <  1:403742/150‑1 (MQ=255)
                              aCGTGGTGGATAAACTAAAACAGCTAAAGGATGTCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTa                                                                                                                        <  2:973957/150‑1 (MQ=255)
                                gtggtgGATAAACTAAAACAGCTATAGGATGCCGAAACTTCGTCGATCCGGTAAAATATCTACCAAACGATGTTCGCCCAGATGATAAAAACGATTGACCTGAATCGTCCTGGCGGTCAGTCCACCGGGGAGATTATGATCTTCGTTtaa                                                                                                                      >  2:123745/1‑150 (MQ=255)
                                 tggtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg                                                                                                                     >  1:293733/1‑150 (MQ=255)
                                  ggtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGt                                                                                                                    >  2:630498/1‑150 (MQ=255)
                                  ggtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGACAGGCAGGacgat                                                                                                                                              <  1:902376/124‑4 (MQ=255)
                                  ggtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGACAGGCAGGacgat                                                                                                                                              >  2:902376/1‑121 (MQ=255)
                                   gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCTATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTc                                                                                                                   <  1:1064592/150‑1 (MQ=255)
                                   gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGAGTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTc                                                                                                                   <  1:1065980/150‑1 (MQ=255)
                                   gtggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAGGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTc                                                                                                                   <  2:910139/150‑1 (MQ=255)
                                    tggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCg                                                                                                                  <  2:293733/150‑1 (MQ=255)
                                    tggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCAGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCg                                                                                                                  >  1:1596278/1‑150 (MQ=255)
                                     ggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCgg                                                                                                                 <  2:789322/150‑1 (MQ=255)
                                     ggATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCgg                                                                                                                 <  1:595131/150‑1 (MQ=255)
                                         aaaCTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACAATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTcc                                                                                                             >  1:482092/1‑150 (MQ=255)
                                             tAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCt                                                                                                         <  1:845677/150‑1 (MQ=255)
                                              aaaaCAGCTAAAGGATGCCGAAACGTTGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCtt                                                                                                        >  2:791334/1‑150 (MQ=255)
                                              aaaaCAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCtt                                                                                                        >  2:8637/1‑150 (MQ=255)
                                               aaaCAGCTAAAGGATGCCGAAACGTCGTCGATGGTGAAAATTATCAACCATACGATATTCTACAACATGCAAAAAGCGATTGACCAGAATAGTCCTGACAGTCAGGACACCGTGGAGCATATGTTATTCGTTAAAGTCAGTTCCCGCTTc                                                                                                       >  1:330328/1‑150 (MQ=255)
                                               aaaCAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCAAACGATGTTCGACAACATGCAAAAAGCGATTGCCCTGAATCGTCCTGCCTGACAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTc                                                                                                       >  1:135323/1‑150 (MQ=255)
                                                 aCAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACGCCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTccc                                                                                                     <  1:374697/150‑1 (MQ=255)
                                                   aGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCAc                                                                                                   <  2:579510/150‑1 (MQ=255)
                                                     cTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACAATACGATGTTCGACAACATGCAAAAAGCGATTTACCTTAATCGTACTGCCTGTCAGTAAACAGGGGATATTATGTTATTAGTTAAAGTCGGTTCCCTATTCCAACTg                                                                                                 >  2:321637/1‑150 (MQ=255)
                                                      aaaaGGATTCCGAAACGTCGTCGATGGGTAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGCCCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGc                                                                                                <  2:76337/149‑1 (MQ=255)
                                                            aTGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCg                                                                                          <  2:300544/150‑1 (MQ=255)
                                                                cGAAACGTCGTCGATGGGGAATATTATCTACCATACGATGGTCCACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCt                                                                                      <  1:935839/150‑1 (MQ=255)
                                                                 gAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTg                                                                                     <  1:410352/150‑1 (MQ=255)
                                                                   aaCGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCa                                                                                   <  1:1239976/150‑1 (MQ=255)
                                                                       tcgtcgATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGACTCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTGCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGc                                                                               <  1:1284706/150‑1 (MQ=255)
                                                                              tGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTCACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCa                                                                        >  2:1039935/1‑150 (MQ=255)
                                                                               ggggAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCaa                                                                       >  2:1022262/1‑150 (MQ=255)
                                                                               ggggAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCaa                                                                       <  2:1311212/150‑1 (MQ=255)
                                                                                 ggAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGCCACCGGGGAGATTATGTTCTTCGTTAACGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAAc                                                                     >  2:1089593/1‑150 (MQ=255)
                                                                                  gAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGAGATTGACCTGAATCGTCCAGCCTGTCATTACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTACCCGCTTCCCACTGCTTGGCGACCTGCAAAGCATACTCAAACa                                                                    >  1:938141/1‑150 (MQ=255)
                                                                                     aaTTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGc                                                                 <  1:429247/150‑1 (MQ=255)
                                                                                         aTCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTg                                                             >  1:1389239/1‑150 (MQ=255)
                                                                                               cATACGATGTTCGACGACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAagag                                                       <  1:538913/150‑1 (MQ=255)
                                                                                               cATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGGCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAagag                                                       <  2:970268/150‑1 (MQ=255)
                                                                                               cATACGATGTGCGACACCATGCAAAAAGCGATTGACCTCAAGCGTCCTGCCTGTCAGGACACCGGGGAGAATATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCCCTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAagag                                                       <  1:288946/150‑1 (MQ=255)
                                                                                                aTACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGACCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGATACCCAATGATTGCCGAGCTGCAAAGCATACACAAACAAGCCGTGGAATAgg                                                      >  2:943739/1‑150 (MQ=255)
                                                                                                aTACGATGTTCGACAACAAGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAgg                                                      <  1:1366602/150‑1 (MQ=255)
                                                                                                  aCGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCa                                                    >  2:291256/1‑150 (MQ=255)
                                                                                                   cGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCac                                                   >  2:1029573/1‑149 (MQ=255)
                                                                                                     aTGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAAcc                                                 >  1:14965/1‑150 (MQ=255)
                                                                                                            acaacaTGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAg                                          >  2:972831/1‑150 (MQ=255)
                                                                                                             caacaTGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAgc                                         <  2:592085/150‑1 (MQ=255)
                                                                                                              aacaTGCAAAACGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAgcg                                        <  2:851276/150‑1 (MQ=255)
                                                                                                              aacaTGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAgcg                                        <  1:134223/150‑1 (MQ=255)
                                                                                                                caTGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGcc                                      >  1:1325509/1‑150 (MQ=255)
                                                                                                                 aTGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCa                                     >  1:1140150/1‑150 (MQ=255)
                                                                                                                  tGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCAc                                    >  1:1411490/1‑150 (MQ=255)
                                                                                                                    cAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTa                                  <  1:429592/150‑1 (MQ=255)
                                                                                                                     aaaaaGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTAc                                 >  1:1474594/1‑150 (MQ=255)
                                                                                                                     aaaaaGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATAATCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTAc                                 >  1:540876/1‑150 (MQ=255)
                                                                                                                           cGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTcaca                           >  1:765599/1‑150 (MQ=255)
                                                                                                                              ttGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATg                        <  2:483762/150‑1 (MQ=255)
                                                                                                                                gACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCg                      >  1:309162/1‑150 (MQ=255)
                                                                                                                                gACATGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAAAAAGCCGTGGAAGAGGCACACGTCAAAGCGCCACTACGTCACAATGCg                      >  2:1495055/1‑150 (MQ=255)
                                                                                                                                 aCCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAATATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCgg                     <  1:96961/150‑1 (MQ=255)
                                                                                                                                  ccTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGt                    >  1:159203/1‑150 (MQ=255)
                                                                                                                                   cTGAATCGTCCTGCCTTTCATTAAACATGTGAGATTATCTTATTCTTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTa                   <  2:1596424/150‑1 (MQ=255)
                                                                                                                                    tGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAg                  >  1:364413/1‑150 (MQ=255)
                                                                                                                                    cccggtgTCCTGACAGGCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCTCAATGCGGTAg                  >  1:929213/7‑150 (MQ=255)
                                                                                                                                      aaTCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACACGCCGTGGAAGAGGCAACCGTCACAGCGCCACTACGTCACAATGCGGTAGaa                >  2:1698832/1‑150 (MQ=255)
                                                                                                                                          gTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAAttt            >  2:1650863/1‑150 (MQ=255)
                                                                                                                                          gTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAAttt            >  1:763151/1‑150 (MQ=255)
                                                                                                                                            cctgcctgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAAttttt          >  1:1008056/1‑150 (MQ=255)
                                                                                                                                            cctgcctgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAAttttt          <  1:39109/150‑1 (MQ=255)
                                                                                                                                             ctgcctgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTg         <  1:1128325/150‑1 (MQ=255)
                                                                                                                                              tgcctgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGa        >  2:475733/1‑150 (MQ=255)
                                                                                                                                               gcctgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCACCCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGAc       >  1:1706392/1‑150 (MQ=255)
                                                                                                                                               gcctgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGAc       <  1:1039935/150‑1 (MQ=255)
                                                                                                                                                cctgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCCCAATGCGGTAGAAATTTTTGACg      >  1:1368119/1‑150 (MQ=255)
                                                                                                                                                 ctgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGa     >  2:749247/1‑150 (MQ=255)
                                                                                                                                                  tgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGaa    >  1:482054/1‑150 (MQ=255)
                                                                                                                                                  tgTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGaa    >  1:1239590/1‑150 (MQ=255)
                                                                                                                                                    tCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGAAGt  <  2:1596278/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATACTCAAACAAGCCGTGGAAGAGGCAACCGTCAAAGCGCCACTACGTCACAATGCGGTAGAAATTTTTGACGAAGT  >  NC_000913/3206527‑3206824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: