Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,208,205 G→A 6.2% G61D (GGT→GAT)  ttdT → L‑tartrate/succinate antiporte

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,208,2050GA 6.2% 306.9 / 6.2 98G61D (GGT→GAT) ttdTL‑tartrate/succinate antiporte
Reads supporting (aligned to +/- strand):  ref base G (35/56);  new base A (2/4);  total (37/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

Only 100 of 105 total aligned reads displayed.

GCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTT  >  NC_000913/3208057‑3208353
                                                                                                                                                    |                                                                                                                                                    
gcgcCGCTGGCGGTCTTCGCCATTATTGCTCTACTTCCGTGTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGAGCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGt                                                                                                                                                     <  2:1497371/150‑1 (MQ=255)
gcgcCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGt                                                                                                                                                     >  1:868038/1‑150 (MQ=255)
gcgcCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGt                                                                                                                                                     <  1:651576/150‑1 (MQ=255)
  gccgcTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGACCCATACCTGGCTCTTCTTTGCGGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGATCCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGtat                                                                                                                                                   >  1:112682/1‑150 (MQ=255)
  gccgcTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGtat                                                                                                                                                   <  2:1192520/150‑1 (MQ=255)
   ccgcTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGtata                                                                                                                                                  >  2:664766/1‑150 (MQ=255)
   ccgcTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGtata                                                                                                                                                  >  2:1389090/1‑150 (MQ=255)
       tGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCca                                                                                                                                              <  2:1372042/150‑1 (MQ=255)
           gggaaTCGCCATTATTGCTCTACTTCAGGTTCCCGCAGGGCTGGAGAAACATACCTGGCTCTACTTTGCCGTTTTTACTGGCTTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGATGGATATACCcaccat                                                                                                                                          <  2:1496641/146‑1 (MQ=255)
            gTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGATGGAtata                                                                                                                                                  <  1:35225/141‑1 (MQ=255)
            gTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGATGGAtata                                                                                                                                                  >  2:35225/1‑141 (MQ=255)
             tCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGATGGATATACCCACCATCg                                                                                                                                        <  2:1669139/150‑1 (MQ=255)
             gcATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGAGCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCg                                                                                                                                        <  2:1141394/149‑1 (MQ=255)
               aTCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGcc                                                                                                                                      <  2:431029/150‑1 (MQ=255)
               aTCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGcc                                                                                                                                      <  1:353266/150‑1 (MQ=255)
                  gCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATa                                                                                                                                   <  1:1451885/150‑1 (MQ=255)
                  gCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATa                                                                                                                                   <  2:1257276/150‑1 (MQ=255)
                   ccATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATAc                                                                                                                                  <  1:447689/150‑1 (MQ=255)
                   ccATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCAGTGCCGGGTGCCGTGGTTGCGATGTTGGGTAAATCCATAATCGCCATAc                                                                                                                                  >  2:331821/1‑150 (MQ=255)
                    cATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGATGGCGATGATGGATATACCCACCATCGCCaccac                                                                                                                                 <  1:227638/150‑5 (MQ=255)
                         ttGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACtctctc                                                                                                                            <  1:22187/150‑1 (MQ=255)
                           gCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTccc                                                                                                                          <  1:585653/150‑1 (MQ=255)
                           gCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTccc                                                                                                                          <  1:1091886/150‑1 (MQ=255)
                             tctACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTg                                                                                                                        >  2:41371/1‑150 (MQ=255)
                                aCTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGgct                                                                                                                     >  1:593639/1‑150 (MQ=255)
                                aCTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGgct                                                                                                                     <  1:1533310/150‑1 (MQ=255)
                                 cTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGgctg                                                                                                                    >  2:1548026/1‑150 (MQ=255)
                                    ccGGTTCCCGCAGGGCTTGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGctgctg                                                                                                                 <  2:766601/150‑1 (MQ=255)
                                    ccGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGctgctg                                                                                                                 >  2:10069/1‑150 (MQ=255)
                                    ccGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGCTATATCCATCATCGCCATACTCTCTCCCTGGctgctg                                                                                                                 <  2:364516/150‑1 (MQ=255)
                                     cGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGt                                                                                                                <  1:1431410/150‑1 (MQ=255)
                                       gatCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTAACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTc                                                                                                              <  2:1001824/148‑1 (MQ=255)
                                        ttCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCa                                                                                                             <  1:1606814/150‑1 (MQ=255)
                                           ccGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGcc                                                                                                          >  1:1378094/1‑150 (MQ=255)
                                           ccGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGcc                                                                                                          >  1:710916/1‑150 (MQ=255)
                                           ccGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGcc                                                                                                          <  2:1523824/150‑1 (MQ=255)
                                           ccGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGGGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGcc                                                                                                          <  2:59179/150‑1 (MQ=255)
                                            cGCAGGGCGGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGccc                                                                                                         <  1:765427/150‑1 (MQ=255)
                                                 ggCTGGAGACTCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACGCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGagc                                                                                                    <  2:1154737/150‑1 (MQ=255)
                                                 ggCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGagc                                                                                                    <  1:391115/150‑1 (MQ=255)
                                                 ggCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGAGCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGagc                                                                                                    <  1:1345387/150‑1 (MQ=255)
                                                         aaCCATACCTGGCTCTACTTTGCCTTTTTTACTGGCGTGATCGTTGGACTGATCCTCGCACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATTCTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAgctcgct                                                                                            <  2:226011/150‑1 (MQ=255)
                                                          aCCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAgctcgctc                                                                                           >  1:1166532/1‑150 (MQ=255)
                                                           ccATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCa                                                                                          >  1:1105138/1‑150 (MQ=255)
                                                            cATACCTGGCTCTACTTTGCCTTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAg                                                                                         <  2:1055794/150‑1 (MQ=255)
                                                              tACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGcc                                                                                       <  1:455340/150‑1 (MQ=255)
                                                              tACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGcc                                                                                       <  1:224579/150‑1 (MQ=255)
                                                              tACATGGATCTACTTTGCAGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGcc                                                                                       <  2:1113780/150‑1 (MQ=255)
                                                                ccTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAg                                                                                     <  1:172223/150‑1 (MQ=255)
                                                                  tGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGc                                                                                   >  1:1422076/1‑150 (MQ=255)
                                                                    gCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCtt                                                                                 >  1:903224/1‑150 (MQ=255)
                                                                     ctctACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGTCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCttt                                                                                <  2:1218313/150‑1 (MQ=255)
                                                                     ctctACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCttt                                                                                <  2:868038/150‑1 (MQ=255)
                                                                     ctctACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCttt                                                                                >  1:1180354/1‑150 (MQ=255)
                                                                     ctctACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATACTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCACTGGCTGCTGTTCAGCCCGGCGCAGCTCGCTCAGCCAGGCttt                                                                                >  1:1376898/1‑150 (MQ=255)
                                                                         aCTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGGTTTAAAt                                                                            >  1:937796/1‑150 (MQ=255)
                                                                            ttGCCGTGTTTTCTGGCGGGATCGTTGGACTGATCCTCGACCCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCATGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCa                                                                         <  1:68561/150‑1 (MQ=255)
                                                                             tGCCGTTTTTACTGGCGTGCTCGTTGGACTGATCCTCGACCCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGGATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCATCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCAc                                                                        <  1:378559/150‑1 (MQ=255)
                                                                              gCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACt                                                                       <  1:922984/150‑1 (MQ=255)
                                                                               ccGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTg                                                                      <  1:1481138/150‑1 (MQ=255)
                                                                               ccGGTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTg                                                                      <  2:114029/150‑1 (MQ=255)
                                                                                 gTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCa                                                                    <  1:553123/150‑1 (MQ=255)
                                                                                   ttgtACTGGCGTGATCGTTGGTCTGATCCTCGAACCCGTGCCGGGGGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCaaa                                                                  <  2:172447/147‑1 (MQ=255)
                                                                                     ttACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAAt                                                                <  2:33267/150‑1 (MQ=255)
                                                                                        cTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATccc                                                             <  1:913691/150‑1 (MQ=255)
                                                                                          ggCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCctc                                                           <  2:608333/150‑1 (MQ=255)
                                                                                          ggCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCctc                                                           <  1:1463787/150‑1 (MQ=255)
                                                                                               gATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTg                                                      >  1:1180195/1‑150 (MQ=255)
                                                                                                       gACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCACCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGttt                                              >  1:220048/1‑150 (MQ=255)
                                                                                                         cTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTcc                                            <  2:1070521/150‑1 (MQ=255)
                                                                                                         cTGATCCTCGAACCCGTGCCGGGTGCCGGGGGGGCGATGGTTGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTcc                                            <  2:444483/150‑1 (MQ=255)
                                                                                                          tGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCGCGCTCAGCAAGGCTTTAATTTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCg                                           >  1:483228/1‑150 (MQ=255)
                                                                                                           gATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCgg                                          >  1:1342264/1‑150 (MQ=255)
                                                                                                           gATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCACTCTCGTGGGCCGTTTCCgg                                          >  2:1142865/1‑150 (MQ=255)
                                                                                                            aTCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGt                                         >  2:588969/1‑150 (MQ=255)
                                                                                                            aTCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGt                                         <  2:1050810/150‑1 (MQ=255)
                                                                                                             tCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCGAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGtt                                        >  2:314655/1‑150 (MQ=255)
                                                                                                             tCCTCGAACCAGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTTAATTCACTCCAAAATCCCTCTCGTGGGCCGTTTACGGtt                                        >  1:180942/1‑150 (MQ=255)
                                                                                                               cTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCCCTGCAAAATCCCTCTCGTGGGCCGTGTCCGGtttt                                      >  1:1172398/1‑150 (MQ=255)
                                                                                                               cTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGtttt                                      >  1:420481/1‑150 (MQ=255)
                                                                                                               cTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATAGCGATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGGCAGGGTTTTAATTGGCTGATAAATCCCTCTCGTGGGCCGTTTCGGGtgtt                                      >  1:1001548/1‑150 (MQ=255)
                                                                                                                tCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGttttt                                     >  1:867550/1‑150 (MQ=255)
                                                                                                                 cGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACGCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTTCAAAATCCCTCTCGTGGGCCGTATCCGGTTTTTc                                    <  1:250556/150‑1 (MQ=255)
                                                                                                                  gAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCt                                   >  2:651055/1‑150 (MQ=255)
                                                                                                                         tGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCgg                            <  1:172317/150‑1 (MQ=255)
                                                                                                                          gCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATAGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGt                           <  2:1393448/150‑1 (MQ=255)
                                                                                                                              ggTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATc                       >  2:500161/1‑150 (MQ=255)
                                                                                                                               gTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCt                      <  2:1105138/150‑1 (MQ=255)
                                                                                                                                tGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTACCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTg                     <  1:829154/150‑1 (MQ=255)
                                                                                                                                        tggCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGAttt             <  2:710916/150‑1 (MQ=255)
                                                                                                                                         ggCGATGATGGATATACCCACCAACGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCACGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTCGCTGAtttt            >  2:1189421/1‑150 (MQ=255)
                                                                                                                                          gCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTc           <  1:1479879/150‑1 (MQ=255)
                                                                                                                                          gCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGGCAGGCGTTAAATTCACTGCAAAATCCCTCTCGGGGGCCGTTTCCGGTTTTGCTAATTCGGCTCTCTGGCTGATTTTcg          <  2:1295356/150‑1 (MQ=255)
                                                                                                                                                  gggTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCtt   <  1:946817/150‑1 (MQ=255)
                                                                                                                                                   ggTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCttt  >  1:753390/1‑150 (MQ=255)
                                                                                                                                                   ggTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCttt  <  2:593639/150‑1 (MQ=255)
                                                                                                                                                   ggTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCttt  <  2:1422076/150‑1 (MQ=255)
                                                                                                                                                   ggTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCttt  >  1:994462/1‑150 (MQ=255)
                                                                                                                                                   ggTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGACTGATTTTCGCCGCCttt  <  2:1342264/150‑1 (MQ=255)
                                                                                                                                                   ggTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGACAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCttt  >  1:976110/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCCTTT  >  NC_000913/3208057‑3208353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: