Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,215,695 A→T 5.3% intergenic (+22/+32) ileX → / ← yqjH tRNA‑Ile/putative siderophore interacting protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,215,6950AT 5.3% 403.8 / 6.2 134intergenic (+22/+32)ileX/yqjHtRNA‑Ile/putative siderophore interacting protein
Reads supporting (aligned to +/- strand):  ref base A (62/64);  new base T (3/5);  total (65/69)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

CGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAA  >  NC_000913/3215549‑3215844
                                                                                                                                                  |                                                                                                                                                      
cgGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAAt                                                                                                                                                     >  2:1126004/1‑150 (MQ=255)
cgGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAAt                                                                                                                                                     >  2:318437/1‑150 (MQ=255)
 ggTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACGAGCTATAGCAAAGGCTGACGAGAAGTc                                                                                                                                                    >  2:204799/1‑150 (MQ=255)
    aaTGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTc                                                                                                                                                 <  1:271079/150‑1 (MQ=255)
       ggCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCGTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGATTTCTCGTCAGc                                                                                                                                              <  1:891549/150‑1 (MQ=25)
       ggCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGATTTCTCGTCAGc                                                                                                                                              <  1:1491932/150‑1 (MQ=255)
        gCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGcc                                                                                                                                             <  1:1409829/150‑1 (MQ=255)
           ggATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCcttt                                                                                                                                          >  1:1699648/1‑150 (MQ=255)
            gATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGATTTCTCGTCAGCctttg                                                                                                                                         <  2:533678/150‑2 (MQ=255)
            gATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttt                                                                                                                                         >  2:541094/1‑150 (MQ=255)
              ttGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCTACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGGCAGCctttttc                                                                                                                                       <  2:1721507/150‑1 (MQ=255)
                  gACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttttctttt                                                                                                                                   <  1:157016/150‑1 (MQ=255)
                    cacGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTta                                                                                                                                 <  2:1022991/150‑1 (MQ=255)
                     acGTAGTTACTTTATAATCCGCGACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTtat                                                                                                                                <  1:1671325/150‑1 (MQ=255)
                      cGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTtata                                                                                                                               <  1:103670/150‑1 (MQ=255)
                        gAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTtatata                                                                                                                             <  1:982561/150‑1 (MQ=255)
                            tACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAg                                                                                                                         <  2:1399092/150‑1 (MQ=255)
                            tACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAg                                                                                                                         >  2:454441/1‑150 (MQ=255)
                            tACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAg                                                                                                                         >  2:481132/1‑150 (MQ=255)
                                ttACAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTAc                                                                                                                     >  2:1694251/1‑150 (MQ=255)
                                   taATCCGCTACCATGGCCCCTTAGCTCAGGGGTTAGCGCAGGCGACTCATTATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCGTTTTATATATCAGTTACttt                                                                                                                  >  2:282850/1‑150 (MQ=255)
                                    aaTCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTg                                                                                                                 <  2:34330/150‑1 (MQ=255)
                                    aaTCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGGTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTg                                                                                                                 <  2:957167/150‑1 (MQ=255)
                                     aTCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATAACAGTTACTTTGc                                                                                                                >  2:630436/1‑150 (MQ=255)
                                        cGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTg                                                                                                             <  1:491736/150‑1 (MQ=255)
                                        cGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTg                                                                                                             <  2:914343/150‑1 (MQ=255)
                                         gCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGc                                                                                                            >  1:237898/1‑150 (MQ=255)
                                            aCCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTAGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAg                                                                                                         <  1:318169/150‑1 (MQ=255)
                                              cATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATTTATCAGTTACTTTGCGTGCCAGTa                                                                                                       >  2:87241/1‑150 (MQ=255)
                                                tGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAg                                                                                                     <  1:709288/150‑1 (MQ=255)
                                                 ggCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTTGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGc                                                                                                    >  2:1130552/1‑150 (MQ=255)
                                                  gCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGcc                                                                                                   <  1:1226560/150‑1 (MQ=255)
                                                   ccccTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCg                                                                                                  >  2:232078/1‑150 (MQ=255)
                                                   ccccTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCg                                                                                                  >  2:588208/1‑150 (MQ=255)
                                                   ccccTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCg                                                                                                  >  2:795008/1‑150 (MQ=255)
                                                       ttACCTCAGTGTTTAGTGCAGTCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGAAGTGGCCACTAGATATAGCAAAGGCTGACGATTAATCTTCAGCCTTTTTCTATTAATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGc                                                                                              <  1:105843/150‑1 (MQ=25)
                                                          gCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACg                                                                                           >  1:211135/1‑150 (MQ=255)
                                                             cAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTAc                                                                                        >  1:1181456/1‑150 (MQ=255)
                                                               ttGGTTAGCGCAGGCGTCGCATAATCGCTTGGGCGCTGGTTCAAGGCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTAccc                                                                                      <  1:733282/149‑1 (MQ=255)
                                                               gTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTAccc                                                                                      <  1:898225/150‑1 (MQ=255)
                                                                 ggTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGAAAAGGCTGACGAGAAATCGTCCGCCTTTTTATTTTTATATATCATTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCAGc                                                                                    >  2:255008/1‑150 (MQ=37)
                                                                    tAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCtgt                                                                                 <  2:693446/150‑1 (MQ=255)
                                                                      gagCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAATTCGTCAGCCTTTGTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTgg                                                                               >  1:327397/1‑150 (MQ=255)
                                                                       agCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCTGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTggg                                                                              <  1:608180/150‑1 (MQ=255)
                                                                       agCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGGCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTggg                                                                              <  1:100777/150‑1 (MQ=255)
                                                                        gCAGGCTACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGt                                                                             <  2:121480/150‑1 (MQ=255)
                                                                        gCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTGTTATATATCAGTTACTTTGCGTGACAGTAAGCCGCTGCACGTACCCGCTGggggg                                                                             >  1:1203713/1‑149 (MQ=255)
                                                                        ccAGGCGACTCATAATCGCGTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGt                                                                             <  1:1073088/149‑1 (MQ=255)
                                                                           ggCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCAt                                                                          >  2:1112046/1‑150 (MQ=255)
                                                                           ggCGACTCATAATAGCTTGGTCGCTGGCTCAAGTCCAGCAGGGGCCACCAGAAAAAGAAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCAt                                                                          <  2:660976/150‑1 (MQ=255)
                                                                             cGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATAc                                                                        >  1:183747/1‑150 (MQ=255)
                                                                                cTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGt                                                                     >  1:1358445/1‑150 (MQ=255)
                                                                                    tAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGCCGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCg                                                                 >  2:332620/1‑150 (MQ=255)
                                                                                     aaTCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGc                                                                >  1:702121/1‑150 (MQ=255)
                                                                                         gCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTATTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGTTCATACTGTTCCGCTTCa                                                            >  2:176242/1‑150 (MQ=255)
                                                                                          cTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTCCCCGCTGTGGGTCATACTGTTCCGCTTaaa                                                           >  1:1060741/1‑150 (MQ=255)
                                                                                          cTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCaa                                                           >  1:938630/1‑150 (MQ=255)
                                                                                          cTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCaa                                                           >  2:1706532/1‑150 (MQ=255)
                                                                                            tGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAg                                                         >  2:1594135/1‑150 (MQ=255)
                                                                                             ggTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAgc                                                        >  2:646366/1‑150 (MQ=255)
                                                                                              gTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAgcg                                                       >  1:618465/1‑150 (MQ=255)
                                                                                                   tGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCggcggc                                                  <  1:1126004/150‑1 (MQ=255)
                                                                                                    ggTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCt                                                 >  1:310401/1‑150 (MQ=255)
                                                                                                    ggTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCt                                                 >  1:881738/1‑150 (MQ=255)
                                                                                                    ggTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCt                                                 <  1:1599377/150‑1 (MQ=255)
                                                                                                     gTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCtt                                                <  1:1694251/150‑1 (MQ=255)
                                                                                                     gTTCAAGACCAGCAGGGGCCACCAGATATAGCAAAGGATGACGAGAAATCGACATCCTTATACTTTTTATATATAAGTTACTTTTCGAGCCAGTAAGCCGCTGCACGTACCCGCTTTGGGTCATACTGTTCCGCTTCAAAGCggcggcat                                                >  2:1300457/1‑148 (MQ=255)
                                                                                                         aaGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAAt                                           >  1:1701311/1‑150 (MQ=255)
                                                                                                         aaGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAAt                                           <  1:1654473/150‑1 (MQ=255)
                                                                                                          aGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAAtt                                          >  1:182415/1‑150 (MQ=255)
                                                                                                           ggccAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTc                                         <  1:1306039/148‑1 (MQ=255)
                                                                                                           gTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTc                                         <  1:481132/150‑1 (MQ=255)
                                                                                                                gcagGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGGCAGCCTTTTGCTTTTTATATATCAGTTCCTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAAc                                    <  1:502562/150‑1 (MQ=255)
                                                                                                                 cagGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACg                                   <  1:975599/150‑1 (MQ=255)
                                                                                                                 cagGGGCCACAAGATATAGCAAAGGCTGACTTGAAATCGTCAGCCTTTTTCTTTTTATATATCTGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACg                                   <  1:252580/150‑1 (MQ=255)
                                                                                                                   ggggCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTACGCTTCAAAGCGGCGGCTTAAATTCTTAACGAc                                 >  2:887322/1‑150 (MQ=255)
                                                                                                                     ggCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAATTTCTTAACGACtt                               >  2:857068/1‑150 (MQ=255)
                                                                                                                     ggCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTATAATTCTTAACGACtt                               >  2:297407/1‑150 (MQ=255)
                                                                                                                         accaGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTAcc                           >  2:1469796/1‑150 (MQ=255)
                                                                                                                          ccaGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCt                          <  1:232078/150‑1 (MQ=255)
                                                                                                                           caGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAAATTCTTAACGACTTTACCt                          >  1:1026236/1‑150 (MQ=255)
                                                                                                                             gATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCg                       >  1:1564348/1‑150 (MQ=255)
                                                                                                                               aataGCAAAGGCTGACGAGAAATCGGCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGGGCCACACACCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTGCAAAGCGGCGGCGTAAATTCTTAACGACATTACCTTCGcc                     <  1:1286333/149‑1 (MQ=255)
                                                                                                                                   gCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTa                             <  1:657019/138‑1 (MQ=255)
                                                                                                                                   gCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGtt                 >  2:972625/1‑150 (MQ=255)
                                                                                                                                    cAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTa                >  1:585135/1‑150 (MQ=255)
                                                                                                                                      aaGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCTCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATc              <  1:817405/150‑1 (MQ=255)
                                                                                                                                       aGGCTGACGATTTCTCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATcc             >  2:1466157/1‑150 (MQ=255)
                                                                                                                                       aGGCTGACGATTTCTCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGATGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCGTAAATTCTTAACGACTTTACCTTCGCCGGTTATcc             >  2:739050/1‑150 (MQ=255)
                                                                                                                                       aGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTACGCCGCTGCACGTACCCGCTGTGGGTCATACTGTACCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATcc             >  1:992665/1‑150 (MQ=255)
                                                                                                                                       aGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATcc             <  2:421915/150‑1 (MQ=255)
                                                                                                                                        ggCTGACGATTTCTCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCa            >  2:1637936/1‑150 (MQ=255)
                                                                                                                                        ggCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCa            >  2:1130885/1‑150 (MQ=255)
                                                                                                                                        ggCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCa            <  1:588208/150‑1 (MQ=255)
                                                                                                                                        ggCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCa            <  2:1601520/150‑1 (MQ=255)
                                                                                                                                        ggCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGGGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCGTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCa            <  1:98735/150‑1 (MQ=255)
                                                                                                                                            gACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATg        <  2:1699648/150‑1 (MQ=255)
                                                                                                                                            gACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTAACCTTCGCCGGTTATCCAGATg        >  2:900099/1‑150 (MQ=255)
                                                                                                                                            gACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGAACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACATTCGCCGGTTATCCAGATg        >  2:1728366/1‑150 (MQ=255)
                                                                                                                                                agaAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGTCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACATTCGCCGGTTATCCAGATGAAGt    >  1:316626/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                      
CGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAA  >  NC_000913/3215549‑3215844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: