Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,216,659 G→T 5.9% intergenic (‑168/‑120) yqjH ← / → yqjI putative siderophore interacting protein/PadR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,216,6590GT 5.9% 444.5 / 13.9 153intergenic (‑168/‑120)yqjH/yqjIputative siderophore interacting protein/PadR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (60/84);  new base T (3/6);  total (63/90)
Fisher's exact test for biased strand distribution p-value = 7.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-02

Only 100 of 158 total aligned reads displayed.

GGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAA  >  NC_000913/3216510‑3216808
                                                                                                                                                     |                                                                                                                                                     
ggTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTg                                                                                                                                                       <  2:927888/150‑1 (MQ=255)
ggTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTg                                                                                                                                                       <  1:1678278/150‑1 (MQ=255)
ggTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTg                                                                                                                                                       >  1:1442515/1‑150 (MQ=255)
  tAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGaa                                                                                                                                                     >  1:254123/1‑150 (MQ=255)
   aaTCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAAc                                                                                                                                                    >  2:1123808/1‑150 (MQ=255)
     tCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACAGATATGTCTATAGTCTGATAATACGCACCGCCTCTTCTCAGTCATCATTACTCAACGCCGGATGCGGCGTGAActc                                                                                                                                                  >  1:1158132/1‑148 (MQ=255)
            tatCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                                                           <  2:144073/150‑1 (MQ=255)
            tatCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACAGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                                                           <  2:121173/150‑1 (MQ=255)
               cTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGCACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                                                                        <  1:1392800/150‑1 (MQ=255)
               cTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                                                                        <  1:1393462/150‑1 (MQ=255)
               cTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGGCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                                        <  1:1519985/150‑1 (MQ=255)
                taaataaaACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                       >  1:561349/1‑150 (MQ=255)
                taaataaaACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                       >  2:889797/1‑150 (MQ=255)
                aaaataaaACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                       <  2:814016/149‑1 (MQ=255)
                     aaaaCTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATAAGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATAAGGCGTTCACGCCGCATCCGGcgttgag                                                                                                                                  <  2:602349/150‑7 (MQ=38)
                      aaaCTCGCAAATTATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATACGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATAAGGCGTTCACGCCGCATCCGGcgttgagt                                                                                                                                 <  2:1423565/150‑8 (MQ=38)
                      aaaCTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACgtt                                                                                                                                 >  1:736834/1‑149 (MQ=255)
                      aaaCTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATAAGGCGTTCACGCCGCATCCGGcgttgagt                                                                                                                                 <  2:595018/150‑8 (MQ=38)
                         cTCGCAAGTGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTga                                                                                                                              <  1:173057/150‑1 (MQ=255)
                          tCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTgag                                                                                                                             <  1:1248644/150‑1 (MQ=255)
                           cGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTgaga                                                                                                                            >  2:868534/1‑150 (MQ=255)
                                 tgataatgatTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTc                                                                                                                      <  2:800244/150‑1 (MQ=255)
                                 tgataatgatTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTc                                                                                                                      >  2:1349233/1‑150 (MQ=255)
                                   ataatgatTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCa                                                                                                                    <  2:1135991/150‑1 (MQ=255)
                                    taatgatTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCAc                                                                                                                   <  2:631960/150‑1 (MQ=255)
                                    taatgatTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCAc                                                                                                                   >  1:308617/1‑150 (MQ=255)
                                        gatTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGttt                                                                                                               <  2:639517/150‑1 (MQ=255)
                                           tGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTGTaaa                                                                                                            >  2:1626685/1‑150 (MQ=255)
                                            gTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTaaaa                                                                                                           >  2:1445736/1‑150 (MQ=255)
                                            gTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTGTaaaa                                                                                                           >  1:1729466/1‑150 (MQ=255)
                                             ttAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCGCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTaaaaa                                                                                                          <  2:1279829/150‑1 (MQ=255)
                                              tAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTaaa                                                                                                            >  2:821365/1‑147 (MQ=255)
                                              tAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTaaa                                                                                                            <  1:821365/147‑1 (MQ=255)
                                                  cATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCAtt                                                                                                     >  2:117570/1‑150 (MQ=255)
                                                     gATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCAttttt                                                                                                  >  2:38336/1‑150 (MQ=255)
                                                     gATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCAttttt                                                                                                  <  2:733628/150‑1 (MQ=255)
                                                       tAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATATGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTac                                                                                                <  1:876104/150‑1 (MQ=255)
                                                       tAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTac                                                                                                <  2:714246/150‑1 (MQ=255)
                                                            gCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTg                                                                                           <  1:219835/150‑1 (MQ=255)
                                                            gCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTGTAAAAATGATTTTTAAACGTg                                                                                           <  1:1626685/150‑1 (MQ=255)
                                                             cAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGc                                                                                          >  2:379760/1‑150 (MQ=255)
                                                             cAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGc                                                                                          <  2:1507375/150‑1 (MQ=255)
                                                               aGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATc                                                                                                        <  2:1575868/134‑1 (MQ=255)
                                                               aGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCaa                                                                                        >  1:1669348/1‑150 (MQ=255)
                                                                      tGTAGAACTTATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAATAATCATTTTTACACTTGCAAGAACGCt                                                                                 <  2:890538/150‑1 (MQ=255)
                                                                      tGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCt                                                                                 <  2:896562/150‑1 (MQ=255)
                                                                        tAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCa                                                                               <  2:879507/150‑1 (MQ=255)
                                                                        tAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCa                                                                               <  2:1669348/150‑1 (MQ=255)
                                                                           aaCTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCTGCGTGAACCCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATAATTTTTACACTTGCAAGAACGCTCatat                                                                            >  2:437391/1‑150 (MQ=255)
                                                                            aCTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCAGGCCTACGTGTGAGATGAGTCAACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATc                                                                           >  2:1039080/1‑150 (MQ=255)
                                                                            aCTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGCCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCatata                                                                           >  2:850377/1‑149 (MQ=255)
                                                                             cTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCa                                                                          <  1:1445736/150‑1 (MQ=255)
                                                                              tGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACATGCAAGAACGCTCATATCAg                                                                         <  1:1007723/150‑1 (MQ=255)
                                                                               gATATGTCTATAGTCTGATAAGTCGAACCGCCTCTGCTCAGGCATCATTACTCAACGCCGGATGCTGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTATTACACTTGCAAGAACGCTCATATCAGa                                                                        <  1:267006/150‑1 (MQ=255)
                                                                               gATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGa                                                                        >  1:855201/1‑150 (MQ=255)
                                                                                atatGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGat                                                                       <  1:1333306/150‑1 (MQ=255)
                                                                                atatGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGat                                                                       <  2:254123/150‑1 (MQ=255)
                                                                                 tatGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGata                                                                      >  1:446908/1‑150 (MQ=255)
                                                                                 tatGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGata                                                                      <  1:1123808/150‑1 (MQ=255)
                                                                                   tGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGAtata                                                                    >  1:577358/1‑150 (MQ=255)
                                                                                    gTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGatatta                                                                   >  1:1594007/1‑148 (MQ=255)
                                                                                       tataGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAAtt                                                                <  1:1714987/150‑1 (MQ=255)
                                                                                        ataGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACTTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTa                                                               <  1:1541616/150‑1 (MQ=255)
                                                                                         taGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAg                                                              <  2:1644706/150‑1 (MQ=255)
                                                                                          aGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGa                                                             <  2:1220934/150‑1 (MQ=255)
                                                                                           gTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGat                                                            <  1:920485/150‑1 (MQ=255)
                                                                                           gTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGat                                                            >  1:1093427/1‑150 (MQ=255)
                                                                                              tGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGatata                                                         >  2:980438/1‑150 (MQ=255)
                                                                                                aTAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATc                                                       <  1:1535051/150‑1 (MQ=255)
                                                                                                aTAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATc                                                       <  2:1173743/150‑1 (MQ=255)
                                                                                                      cGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTa                                                 <  2:1586520/150‑1 (MQ=255)
                                                                                                       gAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTaa                                                >  1:718666/1‑150 (MQ=255)
                                                                                                        aaCCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAg                                               <  2:277103/150‑1 (MQ=255)
                                                                                                        aaCCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGACTTATACGGCCTACTTGTGAGATGAGTCCACGGTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAg                                               <  2:738278/150‑1 (MQ=255)
                                                                                                           cGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAAtttatatc                                            <  1:1525429/150‑7 (MQ=255)
                                                                                                            gCCTCTTCTCAGGCATCATTACTCAACGCCGGATTCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCaaa                                           >  2:903692/1‑150 (MQ=255)
                                                                                                                 ttctCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCATATATAAATTAGATATATCTAATTAAGCAAAaggag                                      <  1:1499064/150‑1 (MQ=255)
                                                                                                                  tctcAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAaggagg                                     <  2:1093427/150‑1 (MQ=255)
                                                                                                                  tctcAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAaggagg                                     <  1:5554/150‑1 (MQ=255)
                                                                                                                    tcAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCt                                   <  2:680320/150‑1 (MQ=255)
                                                                                                                       ggCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGat                                >  2:451679/1‑150 (MQ=255)
                                                                                                                            catTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAg                           <  1:1330390/150‑1 (MQ=255)
                                                                                                                             atTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACAATTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGc                          <  1:389410/150‑1 (MQ=255)
                                                                                                                              ttACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGcc                         <  2:423203/150‑1 (MQ=255)
                                                                                                                                 cTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCcatc                      >  1:1011764/1‑150 (MQ=255)
                                                                                                                                 cTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCAATTTTACCCTTGCAAGAACGCTCATAACAGATATAAATTAGATATATCAAATTAAGCAAAAGGAGGCTGATATGAGCcatc                      >  1:908341/1‑150 (MQ=255)
                                                                                                                                    aaCGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCatcatc                   <  2:180695/150‑1 (MQ=255)
                                                                                                                                     aCGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCAtcatca                  >  1:516776/1‑150 (MQ=255)
                                                                                                                                       gCCGGATTCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACg                <  2:1596073/150‑1 (MQ=255)
                                                                                                                                       gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAg                           >  1:287609/1‑139 (MQ=255)
                                                                                                                                       gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAg                           <  2:287609/139‑1 (MQ=255)
                                                                                                                                         cGGATAAGGCGTTCACGCCGCATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGaa              >  1:470894/1‑150 (MQ=255)
                                                                                                                                          ggATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAg             <  2:663693/150‑1 (MQ=255)
                                                                                                                                           gATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAgg            <  2:855201/150‑1 (MQ=255)
                                                                                                                                             tGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGgt          <  1:38336/150‑1 (MQ=255)
                                                                                                                                              gcggcgTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATCTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGgtg         <  2:1526231/150‑1 (MQ=255)
                                                                                                                                               cggcgTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGgtgt        >  1:813008/1‑150 (MQ=255)
                                                                                                                                               cggcgTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGACATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGgtgt        >  1:1150241/1‑150 (MQ=255)
                                                                                                                                                ggcgTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGtgtt       <  1:780177/150‑1 (MQ=255)
                                                                                                                                                 gcgTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGACATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGtgttg      <  2:1150241/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAA  >  NC_000913/3216510‑3216808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: