Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,216,660 A→C 5.9% intergenic (‑169/‑119) yqjH ← / → yqjI putative siderophore interacting protein/PadR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,216,6600AC 5.9% 442.5 / 14.4 152intergenic (‑169/‑119)yqjH/yqjIputative siderophore interacting protein/PadR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (58/85);  new base C (3/6);  total (61/91)
Fisher's exact test for biased strand distribution p-value = 7.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-01

Only 100 of 156 total aligned reads displayed.

GTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAAC  >  NC_000913/3216511‑3216809
                                                                                                                                                     |                                                                                                                                                     
gTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGa                                                                                                                                                       >  1:1526231/1‑150 (MQ=255)
 tAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGaa                                                                                                                                                      >  1:254123/1‑150 (MQ=255)
   aTCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACg                                                                                                                                                    <  2:1248366/150‑1 (MQ=255)
    tCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACAGATATGTCTATAGTCTGATAATACGCACCGCCTCTTCTCAGTCATCATTACTCAACGCCGGATGCGGCGTGAActc                                                                                                                                                   >  1:1158132/1‑148 (MQ=255)
     cAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGcc                                                                                                                                                  >  2:5554/1‑150 (MQ=255)
      aGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCt                                                                                                                                                 >  2:1330390/1‑150 (MQ=255)
       gATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCtt                                                                                                                                                >  1:1507375/1‑150 (MQ=255)
              cTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGCACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                                                                         <  1:1392800/150‑1 (MQ=255)
              cTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                                                                         <  1:1393462/150‑1 (MQ=255)
              cTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGGCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                                         <  1:1519985/150‑1 (MQ=255)
               taaataaaACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                        >  1:561349/1‑150 (MQ=255)
               aaaataaaACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                        <  2:814016/149‑1 (MQ=255)
                    aaaaCTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATAAGGCGTTCACGCCGCATCCGGcgttgag                                                                                                                                   <  2:889879/150‑7 (MQ=38)
                    aaaaCTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATAAGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATAAGGCGTTCACGCCGCATCCGGcgttgag                                                                                                                                   <  2:602349/150‑7 (MQ=38)
                     aaaCTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACgtt                                                                                                                                  >  1:736834/1‑149 (MQ=255)
                        cTCGCAAGTGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTga                                                                                                                               <  1:173057/150‑1 (MQ=255)
                         tCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTgag                                                                                                                              <  1:1248644/150‑1 (MQ=255)
                           gCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGAt                                                                                                                            >  1:1598085/1‑150 (MQ=255)
                            aaaaTGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATg                                                                                                                           <  2:1430747/149‑1 (MQ=255)
                               atgataatgatTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGt                                                                                                                        <  2:1600866/150‑1 (MQ=255)
                                tgataatgatTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTc                                                                                                                       >  2:1349233/1‑150 (MQ=255)
                                 gataatgatTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCATCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTcc                                                                                                                      <  2:841904/150‑1 (MQ=255)
                                   taatgatTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCAc                                                                                                                    >  1:308617/1‑150 (MQ=255)
                                     atgatTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGt                                                                                                                  <  2:885601/150‑1 (MQ=255)
                                       gatTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGttt                                                                                                                <  2:639517/150‑1 (MQ=255)
                                          tGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTGTaaa                                                                                                             >  2:1626685/1‑150 (MQ=255)
                                           gTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTaaaa                                                                                                            >  2:1445736/1‑150 (MQ=255)
                                           gTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTGTaaaa                                                                                                            >  1:1729466/1‑150 (MQ=255)
                                               aTCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCa                                                                                                        <  1:815085/150‑1 (MQ=255)
                                                tCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCAt                                                                                                       >  2:899403/1‑150 (MQ=255)
                                                 cATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCAtt                                                                                                      >  2:117570/1‑150 (MQ=255)
                                                  aTGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCAttt                                                                                                     <  2:256287/150‑1 (MQ=255)
                                                    gATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCAttttt                                                                                                   >  2:38336/1‑150 (MQ=255)
                                                      tAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATATGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTac                                                                                                 <  1:876104/150‑1 (MQ=255)
                                                      tAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTac                                                                                                 <  2:714246/150‑1 (MQ=255)
                                                         aTGCAAGGGATTTGTCGATCTGATATGTCTATAGTCTGCTAAGACGAACCGCCTCTTCTCAGGCATGATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACt                                                                                              <  2:865431/150‑1 (MQ=39)
                                                           gCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTg                                                                                            <  1:219835/150‑1 (MQ=255)
                                                           gCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTg                                                                                            <  2:1652174/150‑1 (MQ=255)
                                                           gCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTGTAAAAATGATTTTTAAACGTg                                                                                            <  1:1626685/150‑1 (MQ=255)
                                                            cAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTga                                                                                           >  2:1499064/1‑149 (MQ=255)
                                                              aGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATc                                                                                                         >  1:1575868/1‑134 (MQ=255)
                                                               gCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAg                                                                                        >  2:1168623/1‑150 (MQ=255)
                                                                     tGTAGAACTTATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAATAATCATTTTTACACTTGCAAGAACGCt                                                                                  <  2:890538/150‑1 (MQ=255)
                                                                      gTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTc                                                                                 <  2:1199625/150‑1 (MQ=255)
                                                                       tAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCa                                                                                <  2:879507/150‑1 (MQ=255)
                                                                        aGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCat                                                                               <  1:889797/150‑1 (MQ=255)
                                                                          aaCTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCTGCGTGAACCCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATAATTTTTACACTTGCAAGAACGCTCatat                                                                             >  2:437391/1‑150 (MQ=255)
                                                                           aCTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGCCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCatata                                                                            >  2:850377/1‑149 (MQ=255)
                                                                            cTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCa                                                                           <  1:1445736/150‑1 (MQ=255)
                                                                             tGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACATGCAAGAACGCTCATATCAg                                                                          <  1:1007723/150‑1 (MQ=255)
                                                                              gATATGTCTATAGTCTGATAAGTCGAACCGCCTCTGCTCAGGCATCATTACTCAACGCCGGATGCTGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTATTACACTTGCAAGAACGCTCATATCAGa                                                                         <  1:267006/150‑1 (MQ=255)
                                                                              gATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGa                                                                         >  1:855201/1‑150 (MQ=255)
                                                                               atatGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGat                                                                        <  1:1333306/150‑1 (MQ=255)
                                                                                tatGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGata                                                                       <  1:1123808/150‑1 (MQ=255)
                                                                                tatGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGata                                                                       >  1:446908/1‑150 (MQ=255)
                                                                                 atGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGTCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGatat                                                                      <  1:1494399/150‑1 (MQ=255)
                                                                                  tGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGAtata                                                                     <  2:1120766/150‑1 (MQ=255)
                                                                                  tGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGAtata                                                                     >  1:577358/1‑150 (MQ=255)
                                                                                      tataGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAAtt                                                                 <  1:1714987/150‑1 (MQ=255)
                                                                                        taGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAg                                                               <  1:751749/150‑1 (MQ=255)
                                                                                         aGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGa                                                              <  2:1220934/150‑1 (MQ=255)
                                                                                          gTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGat                                                             >  1:1093427/1‑150 (MQ=255)
                                                                                          gTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGat                                                             >  1:1675849/1‑150 (MQ=255)
                                                                                          gTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGat                                                             <  1:920485/150‑1 (MQ=255)
                                                                                          gTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCAGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTAATATCAGATATAAATTAGat                                                             >  2:1540518/1‑150 (MQ=255)
                                                                                           tCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGata                                                            <  2:175551/150‑1 (MQ=255)
                                                                                           tCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGata                                                            <  2:429927/150‑1 (MQ=255)
                                                                                            cTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGatat                                                           >  2:1582247/1‑150 (MQ=255)
                                                                                             tGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGatata                                                          >  2:980438/1‑150 (MQ=255)
                                                                                               aTAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATc                                                        <  1:1535051/150‑1 (MQ=255)
                                                                                               aTAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATc                                                        <  2:721193/150‑1 (MQ=255)
                                                                                                     cGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTa                                                  <  2:1586520/150‑1 (MQ=255)
                                                                                                       aaCCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAg                                                <  2:277103/150‑1 (MQ=255)
                                                                                                       aaCCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGACTTATACGGCCTACTTGTGAGATGAGTCCACGGTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAg                                                <  2:738278/150‑1 (MQ=255)
                                                                                                        aCCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGc                                               >  2:620870/1‑150 (MQ=255)
                                                                                                          cGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCaa                                             >  2:1127237/1‑150 (MQ=255)
                                                                                                           gCCTCTTCTCAGGCATCATTACTCAACGCCGGATTCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCaaa                                            >  2:903692/1‑150 (MQ=255)
                                                                                                            ccTCTTCTCAGGCATCATTACTCACCGCCGGATGCGGCGTGAACGCCTTATACGGCCTCCGTGTGAGATGAGTCCACTTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCaaaa                                           <  2:319870/150‑1 (MQ=255)
                                                                                                                 tctcAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAaggagg                                      <  2:1093427/150‑1 (MQ=255)
                                                                                                                 tctcAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAaggagg                                      <  1:5554/150‑1 (MQ=255)
                                                                                                                          tcatTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGa                             >  1:1277052/1‑150 (MQ=255)
                                                                                                                            atTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGc                           <  2:1038947/150‑1 (MQ=255)
                                                                                                                            atTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACAATTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGc                           <  1:389410/150‑1 (MQ=255)
                                                                                                                                cTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCcatc                       >  2:739514/1‑150 (MQ=255)
                                                                                                                                cTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCcatc                       >  1:1011764/1‑150 (MQ=255)
                                                                                                                                  cAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCcatcat                     >  2:665929/1‑150 (MQ=255)
                                                                                                                                   aaCGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCatcatc                    <  2:180695/150‑1 (MQ=255)
                                                                                                                                    aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCAtcatca                   >  1:652619/3‑150 (MQ=255)
                                                                                                                                    aCGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCAtcatca                   >  1:516776/1‑150 (MQ=255)
                                                                                                                                      gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAg                            >  1:287609/1‑139 (MQ=255)
                                                                                                                                         ggATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAg              <  1:451679/150‑1 (MQ=255)
                                                                                                                                            tGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGgt           <  1:38336/150‑1 (MQ=255)
                                                                                                                                             gcggcgTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATCTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGgtg          <  2:1526231/150‑1 (MQ=255)
                                                                                                                                              cggcgTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGgtgt         >  1:813008/1‑150 (MQ=255)
                                                                                                                                              cggcgTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGACATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGgtgt         >  1:1150241/1‑150 (MQ=255)
                                                                                                                                                gcgTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGtgttg       <  2:813008/150‑1 (MQ=255)
                                                                                                                                                gcgTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGACATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGtgttg       <  2:1150241/150‑1 (MQ=255)
                                                                                                                                                    gAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTaaa   <  2:643387/150‑1 (MQ=255)
                                                                                                                                                     aaCGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAAc  <  2:577358/150‑1 (MQ=255)
                                                                                                                                                     aaCGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAAc  <  2:308617/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAAGACGAACCGCCTCTTCTCAGGCATCATTACTCAACGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGAGATGAGTCCACGTTTAAAAATCATTTTTACACTTGCAAGAACGCTCATATCAGATATAAATTAGATATATCTAATTAAGCAAAAGGAGGCTGATATGAGCCATCATCACGAAGGGTGTTGTAAAC  >  NC_000913/3216511‑3216809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: