Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,234,702 A→C 5.0% intergenic (‑247/+37) higB ← / ← rlmG mRNA interferase toxin of the HigB‑HigA toxin‑antitoxin system/23S rRNA m(2)G1835 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,234,7020AC 5.0% 380.7 / 9.4 140intergenic (‑247/+37)higB/rlmGmRNA interferase toxin of the HigB‑HigA toxin‑antitoxin system/23S rRNA m(2)G1835 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (68/65);  new base C (2/5);  total (70/70)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.18e-01

Only 100 of 141 total aligned reads displayed.

AAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGAC  >  NC_000913/3234555‑3234851
                                                                                                                                                   |                                                                                                                                                     
aaaCGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAgg                                                                                                                                                     <  1:796877/150‑1 (MQ=255)
aaaCGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAgg                                                                                                                                                     <  2:469278/150‑1 (MQ=255)
  aCGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCg                                                                                                                                                   >  1:904703/1‑150 (MQ=255)
   cGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGt                                                                                                                                                  >  2:56585/1‑150 (MQ=255)
     cTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGttt                                                                                                                                                <  2:1318081/150‑1 (MQ=255)
       aaaCAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTAc                                                                                                                                              >  2:210014/1‑150 (MQ=255)
          cAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                           <  2:237586/150‑1 (MQ=255)
           aaGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                          <  2:1017647/150‑1 (MQ=255)
           aaGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGACTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                          >  2:1664375/1‑150 (MQ=255)
            agagAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTAcgccgc                                                                                                                                         <  2:355206/150‑1 (MQ=255)
             gagaATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGTCCGGATAAGGCGTTTACGCCGCa                                                                                                                                        >  1:179405/1‑150 (MQ=255)
                aaTGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                     <  2:695974/150‑1 (MQ=39)
                  tGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGCACTGGGCAGACATTGACGGATGAGACTCTTGCCGCGTCTTATCCTGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                                                                                                   >  1:265923/1‑150 (MQ=39)
                     aGACGATGGTGACAGGGATTTTTTGTTTTATGGATGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                                                >  2:1122502/1‑150 (MQ=255)
                     aGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcct                                                                                                                                <  2:446967/150‑3 (MQ=39)
                     aGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                                                <  1:1150718/150‑1 (MQ=255)
                      gACGATGGTGACAGTGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACTTGCTTAACGTAGGCCAGATAAGGCGTTTACGCAGCATCCGGCAgg                                                                                                                               >  2:334501/1‑150 (MQ=39)
                      gACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGccta                                                                                                                               <  1:1023246/150‑4 (MQ=39)
                      gACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAgg                                                                                                                               >  2:191313/1‑150 (MQ=255)
                      gACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAgg                                                                                                                               <  1:113255/150‑1 (MQ=255)
                        cGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTa                                                                                                                             <  1:68990/150‑1 (MQ=255)
                          aTGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAAc                                                                                                                           <  1:1673914/150‑1 (MQ=255)
                          aTGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAAc                                                                                                                           >  2:1238170/1‑150 (MQ=255)
                           tGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACt                                                                                                                          >  1:1433331/1‑150 (MQ=255)
                           tGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACt                                                                                                                          >  1:1403423/1‑150 (MQ=255)
                            ggTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTc                                                                                                                         <  1:701564/150‑1 (MQ=255)
                             gTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATTCGGCAGGTAACTcc                                                                                                                        <  2:392356/150‑1 (MQ=255)
                              tGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTTCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGTCGTTTACGCCGCATCCGGCAGGTATCTccc                                                                                                                       >  1:31926/1‑150 (MQ=255)
                                  aGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGATTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCACAATc                                                                                                                   >  2:312744/1‑150 (MQ=255)
                                   gggATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCt                                                                                                                  >  1:460054/1‑150 (MQ=255)
                                   gggATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCt                                                                                                                  >  1:1009380/1‑150 (MQ=255)
                                    ggATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCtt                                                                                                                 >  2:1634021/1‑150 (MQ=255)
                                     taTTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCAGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACACCCAATCTTa                                                                                                                >  1:597626/2‑150 (MQ=255)
                                        tttttGTTTTATGGAAGCGCGATTCCAGATCTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACcg                                                                                                             <  2:1487812/150‑1 (MQ=255)
                                        tttttGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCTATCTTACcg                                                                                                             >  2:261479/1‑150 (MQ=255)
                                                ttATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTc                                                                                                     >  2:563368/1‑150 (MQ=255)
                                                  ttGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGAGGCTTGCCGCGTATTATCCGGACTTGAGTAACCTGCTGAACGTAGGCCGGATAAGGCGTGTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGcccc                                                                                                   <  1:87744/149‑1 (MQ=255)
                                                    ggAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAg                                                                                                 >  1:1169265/1‑150 (MQ=255)
                                                    ggAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAg                                                                                                 >  2:607417/1‑150 (MQ=255)
                                                     gAATCGCGATTACATTAATGGGCATACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGc                                                                                                <  1:317230/150‑1 (MQ=38)
                                                     gAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGc                                                                                                <  1:1097355/150‑1 (MQ=255)
                                                      aaGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCt                                                                                               <  2:52709/150‑1 (MQ=255)
                                                        gcgcGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCGTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTGCCAATCTTACCGACGCCGTCCCAGCTTc                                                                                             >  2:1353701/1‑150 (MQ=255)
                                                        gcgcGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTc                                                                                             <  1:1210653/150‑1 (MQ=255)
                                                           cgATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCAcc                                                                                          <  1:1687988/150‑1 (MQ=255)
                                                               tCCAGAACTGGGCAGACATTGCCGGATGCGACGCTGGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCtt                                                                                      <  2:850276/150‑1 (MQ=255)
                                                                ccAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGGCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGCCGTCGCAGCTGCACCGcggt                                                                                     >  2:816811/1‑147 (MQ=38)
                                                                ccAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCttt                                                                                     >  2:1705072/1‑150 (MQ=255)
                                                                ccAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCttt                                                                                     <  2:1565643/150‑1 (MQ=255)
                                                                 cAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTc                                                                                    <  2:888543/150‑1 (MQ=255)
                                                                  tgtaCTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCTGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCa                                                                                   <  2:1594585/147‑1 (MQ=255)
                                                                   gAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAg                                                                                  <  1:535120/150‑1 (MQ=255)
                                                                     aCTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTa                                                                                >  1:1370665/1‑150 (MQ=255)
                                                                      cTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTAc                                                                               <  2:633/150‑1 (MQ=255)
                                                                       tGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCGTCCCCGCTGTCAGTAcc                                                                              >  1:1530816/1‑150 (MQ=255)
                                                                         ggCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACcac                                                                            >  1:21823/1‑150 (MQ=255)
                                                                           cAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGCCGACGTCCCAGCTGCACCGCGTTCAGTACCACaa                                                                          >  1:1578143/1‑150 (MQ=255)
                                                                             gACATTGCCGGATGCGACGCTTGCCGCTTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGGTTACGCCGCATCCGGCATGTAACTCCCAATCTTACCGACGACTTCCCAGATTCACCGCTTTCAGTACCACAAAt                                                                        <  2:23070/150‑1 (MQ=39)
                                                                                 ttGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATttat                                                                    <  2:1365517/150‑1 (MQ=255)
                                                                                 ttGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATttat                                                                    <  1:958287/150‑1 (MQ=255)
                                                                                  tGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATttatt                                                                   >  1:1686549/1‑150 (MQ=255)
                                                                                    ccGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTtattat                                                                 >  2:781648/1‑150 (MQ=255)
                                                                                           cGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGGAACTCCCAATCTTCCCGACGCCGTCCCAGCTGCACCGCGTTCAGTAGCACAAATTTTTTTTTGGTCGc                                                          >  2:1538456/1‑150 (MQ=38)
                                                                                                  tGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAGCTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCGTTCAGTACCACAAATTTATTATTCGTCGCAATAgtg                                                   >  2:986202/1‑150 (MQ=255)
                                                                                                   gCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCCGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAgtgg                                                  <  1:970745/150‑1 (MQ=255)
                                                                                                   gCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAgtgg                                                  <  1:977636/150‑1 (MQ=255)
                                                                                                    ccGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAgtggt                                                 >  1:175272/1‑150 (MQ=255)
                                                                                                     cgcgTCTTATCCGGCCTGGAGTAACGTGCTGACCGTAGGCCGGATAAGGCGTTTACGCTGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGGAATAgtggtg                                                >  2:471002/1‑150 (MQ=255)
                                                                                                     cgcgTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAgtggtg                                                <  1:383364/150‑1 (MQ=255)
                                                                                                     cgcgTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAgtggtg                                                <  2:1256124/150‑1 (MQ=255)
                                                                                                      gcgTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGc                                               <  1:56585/150‑1 (MQ=255)
                                                                                                         tCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGt                                            <  2:1458117/150‑1 (MQ=255)
                                                                                                           ttATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTg                                          <  2:1192653/150‑1 (MQ=255)
                                                                                                            tATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGc                                         >  1:644439/1‑150 (MQ=255)
                                                                                                             aTCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGcc                                        >  2:989168/1‑150 (MQ=255)
                                                                                                                cGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGaa                                     <  1:1270241/150‑1 (MQ=255)
                                                                                                                   ccTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCGTCCCCGCTTTCGGTTCGACAAATTTTTTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAAt                                  >  2:413744/1‑150 (MQ=39)
                                                                                                                     tGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAAtct                                <  2:768074/150‑1 (MQ=255)
                                                                                                                      ggAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAAtctt                               >  1:422667/1‑150 (MQ=255)
                                                                                                                        aGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAAtcttct                             <  2:408457/150‑1 (MQ=255)
                                                                                                                          tAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCttcttc                           <  1:430810/150‑1 (MQ=255)
                                                                                                                            aCGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAg                         <  2:800018/150‑1 (MQ=255)
                                                                                                                            aCGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAg                         <  1:563368/150‑1 (MQ=255)
                                                                                                                             cGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGt                        <  2:1403423/150‑1 (MQ=255)
                                                                                                                             cGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGt                        <  2:1433331/150‑1 (MQ=255)
                                                                                                                              gTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGtt                       <  2:1009380/150‑1 (MQ=255)
                                                                                                                               tGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCAAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTAAGttt                      >  1:831965/1‑150 (MQ=255)
                                                                                                                                 cTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTAt                    >  1:1187323/1‑150 (MQ=255)
                                                                                                                                  tGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATg                   <  2:756135/150‑1 (MQ=255)
                                                                                                                                   gAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATgg                  >  2:112051/1‑150 (MQ=255)
                                                                                                                                      cGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCTATCTTACCGACGACGTCGCAGCTTCACCGCTTTCAGTACGACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAg               <  1:257468/150‑1 (MQ=255)
                                                                                                                                       gTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGt              <  2:767060/150‑1 (MQ=255)
                                                                                                                                        tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTa             >  1:1028947/1‑150 (MQ=255)
                                                                                                                                         ctgCCGGATGCGGCGTAAACGCCTTATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTaa            >  1:744599/3‑150 (MQ=255)
                                                                                                                                         aGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTACCTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATATATGAGTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTAAGTTTATGGAAGTaa            >  1:260498/1‑150 (MQ=255)
                                                                                                                                          ggCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAAt           >  2:1712476/1‑150 (MQ=255)
                                                                                                                                                aTAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGt     >  1:490686/1‑150 (MQ=255)
                                                                                                                                                 tAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTAGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTg    >  2:450702/1‑150 (MQ=255)
                                                                                                                                                 tAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGAAGTCCCAGCTTCACCGCTTTCAGTTCCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTg    <  1:208026/150‑1 (MQ=255)
                                                                                                                                                   aGGGGTTTACGCGGGATCCGGCAGGTAACTCCCAATCTTACCGACGACGTACCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGAc  <  2:597626/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTGTTTTATGGAAGCGCGATTCCAGAACTGGGCAGACATTGCCGGATGCGACGCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGAC  >  NC_000913/3234555‑3234851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: