Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,238,463 C→T 5.9% intergenic (+166/‑117) ygjR → / → alx putative NAD(P)‑dependent dehydrogenase/putative membrane‑bound redox modulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,238,4630CT 5.9% 429.9 / 9.4 134intergenic (+166/‑117)ygjR/alxputative NAD(P)‑dependent dehydrogenase/putative membrane‑bound redox modulator
Reads supporting (aligned to +/- strand):  ref base C (68/58);  new base T (5/3);  total (73/61)
Fisher's exact test for biased strand distribution p-value = 7.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

GTGTAAAAGGTATGTAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGG  >  NC_000913/3238314‑3238609
                                                                                                                                                     |                                                                                                                                                  
gtgtATAAGGTATGTAACAGACCATTGACTGGCTGAATCGTCTGTCATACTTAGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTATATCGTCATTACGATGTGTGAAAAAACACATCCGGTCAGCGGGCAACGCGAAATGAATACGc                                                                                                                                                    <  1:253032/150‑1 (MQ=255)
  gtAAAAGGTATGTAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAg                                                                                                                                                  >  1:917250/1‑150 (MQ=255)
     aaaGGTATGTAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACg                                                                                                                                               >  1:108452/1‑150 (MQ=255)
           aTGTAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTcc                                                                                                                                         >  1:574458/1‑150 (MQ=255)
           aTGTAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGAAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTcc                                                                                                                                         <  1:114101/150‑1 (MQ=255)
            tGTAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATACGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCt                                                                                                                                        <  2:1234105/150‑1 (MQ=255)
             ttAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCAGAAAGGAATACGCAGACGTATTCCtt                                                                                                                                       <  1:1287446/149‑1 (MQ=255)
             gTAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCtt                                                                                                                                       >  1:106103/1‑150 (MQ=255)
             gTAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCtt                                                                                                                                       <  2:614791/150‑1 (MQ=255)
              tAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAATGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTTAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCttt                                                                                                                                      <  1:131130/150‑1 (MQ=255)
                aCAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGTCTGCGTATTCCtttcg                                                                                                                                    <  2:1685338/150‑3 (MQ=255)
                aCAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCttttt                                                                                                                                    >  1:728547/1‑150 (MQ=255)
                 cAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCtttttt                                                                                                                                   <  2:367327/150‑1 (MQ=255)
                 cAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCtttttt                                                                                                                                   >  2:376041/1‑150 (MQ=255)
                  aGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTttg                                                                                                                                  <  2:702588/150‑1 (MQ=255)
                    aCCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACAGGGCAACCCGAAAGGAATAAGCAGACGTATACCTTTTttgtt                                                                                                                                >  1:1469550/1‑150 (MQ=255)
                         tGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAg                                                                                                                           >  1:185/1‑150 (MQ=255)
                           aCTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTg                                                                                                                         <  2:1713960/150‑1 (MQ=255)
                             tGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATCCGCAGACGTATTCCTTTTTTGTTGTAAGTgag                                                                                                                       >  2:1642330/1‑150 (MQ=255)
                             tGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTgag                                                                                                                       <  1:1592214/150‑1 (MQ=255)
                                cTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGAcc                                                                                                                    <  2:1384627/150‑1 (MQ=255)
                                    aTGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGc                                                                                                                <  1:1068073/150‑1 (MQ=255)
                                      ggTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACCCATCCGGTCACCGGGCAACCCGAAATGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGAACTTGCCg                                                                                                              >  2:1071656/1‑150 (MQ=255)
                                        tcagTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATAGTAATTACGATGTGTGAAAAAACACATCCGGACACCGGGCAACCCGAAAGCAATACGCAGACGTATTCCTTTTTTGATGTAAGTGAGACCTTGCCGGa                                                                                                            >  1:1709703/4‑150 (MQ=255)
                                           gTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAgg                                                                                                         >  1:1097488/1‑150 (MQ=255)
                                            tCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGc                                                                                                        <  1:1553586/150‑1 (MQ=255)
                                            gcATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAATAACTCATCCGGTCACCGGGCAACCCGAAAGGAATACGCTGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGc                                                                                                        <  1:1136704/149‑1 (MQ=255)
                                              aTACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGa                                                                                                      <  2:858299/150‑1 (MQ=255)
                                               tACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAg                                                                                                     >  1:1128117/1‑150 (MQ=255)
                                               tACTTTGTTACCTGCAAAGGGGAGTAACTTCATAGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGAGAGACCTTGCCGGAAGGCGAg                                                                                                     >  1:1116822/1‑150 (MQ=255)
                                                aCTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAgg                                                                                                    <  2:1497398/150‑1 (MQ=255)
                                                 cTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGt                                                                                                   <  1:117611/150‑1 (MQ=255)
                                                  tttGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTTCGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTc                                                                                                  <  1:958377/150‑1 (MQ=255)
                                                    tGTTACCTGCAAAGGGGAGTAACTTCATTGCCCGTCGAACGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTa                                                                                                <  1:440091/150‑1 (MQ=255)
                                                      ttACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATg                                                                                              <  2:1184412/150‑1 (MQ=255)
                                                      ttACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATg                                                                                              <  2:82232/150‑1 (MQ=255)
                                                           tGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATaa                                                                                         >  1:891702/1‑150 (MQ=255)
                                                           tGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATaa                                                                                         >  1:891704/1‑150 (MQ=255)
                                                             cAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATaaaa                                                                                       <  1:1687205/150‑1 (MQ=255)
                                                                 ggggAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAagca                                                                                   >  2:1369601/1‑150 (MQ=255)
                                                                    gAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAACACACATCCGGTCACCGGGCACCCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGAAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGAAGCg                                                                                >  1:1538255/1‑150 (MQ=255)
                                                                     aGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCgg                                                                               >  2:335766/1‑150 (MQ=255)
                                                                      gTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGc                                                                              >  2:1512323/1‑150 (MQ=255)
                                                                      gTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGc                                                                              >  1:738232/1‑150 (MQ=255)
                                                                        aaCTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAATGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTg                                                                            <  2:966648/150‑1 (MQ=255)
                                                                         aCTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGa                                                                           <  1:1087187/150‑1 (MQ=255)
                                                                          cTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGAc                                                                          >  1:1396718/1‑150 (MQ=255)
                                                                                 gCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTcc                                                                   <  1:497514/150‑1 (MQ=255)
                                                                                 gCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTcc                                                                   <  2:421725/150‑1 (MQ=255)
                                                                                 gCCGGTCGATCGACATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTcc                                                                   <  1:734714/150‑1 (MQ=255)
                                                                                  ccGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCGGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCg                                                                  <  2:703263/150‑1 (MQ=255)
                                                                                    ggTCGATCGTCATTACGATGAGTGAAAAAACAAATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTATTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGCCTTCTTCCgcc                                                                >  2:314623/1‑148 (MQ=255)
                                                                                     gTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACg                                                               <  2:935395/150‑1 (MQ=255)
                                                                                      tctaTCGTCAGTAAGATGTTTGACAAAACACATTCGGTCACCGGTCAACCCGAAAGGAATACGCAGACGTATGCCTTTTTTGTTGTAAGTTAGACCTTGCCTGCAGGCGAGGTCTATGCATATAAAGCAGCGGCTGACTTATTCCGAAGt                                                              <  1:107199/147‑1 (MQ=255)
                                                                                      tCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGt                                                              >  1:1104417/1‑150 (MQ=255)
                                                                                         aTCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTgg                                                           >  2:1480405/1‑150 (MQ=255)
                                                                                         aTCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTgg                                                           <  1:335766/150‑1 (MQ=255)
                                                                                         aTCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTgg                                                           >  1:12001/1‑150 (MQ=255)
                                                                                           cGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGcc                                                         >  1:770979/1‑150 (MQ=255)
                                                                                              cATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtt                                                      >  1:1338096/1‑150 (MQ=255)
                                                                                               aTTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGttt                                                     >  1:15355/1‑150 (MQ=255)
                                                                                                ttACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttt                                                    >  2:569381/1‑150 (MQ=255)
                                                                                                  aCGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                  <  1:591910/150‑1 (MQ=255)
                                                                                                  aCGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACACAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt                                                  >  1:1362173/1‑150 (MQ=255)
                                                                                                   cGATGTGTGAAAAAACACATCCGGTCACCGGGCACCCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGAAGCGGCTGACTTCTTCCGACGTTGGCCGgtttttt                                                 >  2:307638/1‑150 (MQ=255)
                                                                                                   cGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGCCGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGCCGTTGGCCGttttttt                                                 >  2:626470/1‑150 (MQ=255)
                                                                                                   cGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGttttttt                                                 <  1:1514208/150‑1 (MQ=255)
                                                                                                    gATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTa                                                <  2:1688811/150‑1 (MQ=255)
                                                                                                      tgtgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTAtg                                              >  1:1143539/1‑150 (MQ=255)
                                                                                                      tgtgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTAtg                                              <  2:55950/150‑1 (MQ=255)
                                                                                                      tgtgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTAtg                                              <  1:1601012/150‑1 (MQ=255)
                                                                                                        tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTAtgtg                                            <  1:1098465/150‑1 (MQ=255)
                                                                                                         gtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATgtgt                                           >  2:1111778/1‑150 (MQ=255)
                                                                                                          tgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTa                                          >  2:1028730/1‑150 (MQ=255)
                                                                                                           gAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTaa                                         >  1:1729489/1‑150 (MQ=255)
                                                                                                            aaaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAg                                        <  1:482490/150‑1 (MQ=255)
                                                                                                                  cacaTCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACtt                                  >  1:1644366/1‑150 (MQ=255)
                                                                                                                  cacaTCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACtt                                  >  2:82575/1‑150 (MQ=255)
                                                                                                                   acaTCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTc                                 >  2:680714/1‑150 (MQ=255)
                                                                                                                    caTCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGACTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCt                                >  1:339568/1‑150 (MQ=255)
                                                                                                                       ccGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATg                             <  2:770979/150‑1 (MQ=255)
                                                                                                                           tCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATa                         <  2:1184545/150‑1 (MQ=255)
                                                                                                                            cACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATaa                        >  2:1300368/1‑149 (MQ=255)
                                                                                                                            cACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGACGGAAGGCGAGGTCTATGCTTATAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATAc                        >  1:602958/1‑150 (MQ=255)
                                                                                                                                gggCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTc                    >  1:215490/1‑150 (MQ=255)
                                                                                                                                 ggCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCg                   <  1:942515/150‑1 (MQ=255)
                                                                                                                                   cAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGc                 <  2:1550292/150‑1 (MQ=255)
                                                                                                                                    aacaaaaaaGGAATACGTCTGCGTATTCCTTTCTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                >  1:1044710/7‑150 (MQ=255)
                                                                                                                                    aaCCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCa                >  1:1050337/1‑150 (MQ=255)
                                                                                                                                      cccGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACg              >  2:230153/1‑150 (MQ=255)
                                                                                                                                      cccGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACg              >  1:13789/1‑150 (MQ=255)
                                                                                                                                       aaaaaaGGAATACGTCTGCGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGc             >  1:1461296/4‑150 (MQ=255)
                                                                                                                                       aaaaaaGGAATACGTCTGCGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGc             >  2:1168501/4‑150 (MQ=255)
                                                                                                                                        cGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGcc            >  2:1089512/1‑150 (MQ=255)
                                                                                                                                         aaaaGGAATACGTCTGCGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAATAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTTTTTGTAAGGAACTTCTATGAATACTGTCGGCACGCCg           >  1:349734/2‑150 (MQ=255)
                                                                                                                                           aaGGAATACGTCTGCGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGAACGCCGtt         >  2:1531366/1‑150 (MQ=255)
                                                                                                                                           aaGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCTACGTTGGCCGTTTTATTATGTGTAAGGAACTTCTATGACTACTGTCGGCACGCCGtt         >  1:180030/1‑150 (MQ=255)
                                                                                                                                           aaGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTATTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGtt         >  2:242271/1‑150 (MQ=255)
                                                                                                                                             ggAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGc       >  2:399018/1‑150 (MQ=255)
                                                                                                                                                  aCGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATgg  <  1:1008169/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GTGTAAAAGGTATGTAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGG  >  NC_000913/3238314‑3238609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: