Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,241,781 C→T 6.0% intergenic (‑37/+46) ygjV ← / ← uxaA Imp‑YgjV family inner membrane protein/altronate hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,241,7810CT 6.0% 369.1 / 7.0 115intergenic (‑37/+46)ygjV/uxaAImp‑YgjV family inner membrane protein/altronate hydrolase
Reads supporting (aligned to +/- strand):  ref base C (59/49);  new base T (3/4);  total (62/53)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

AGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATA  >  NC_000913/3241636‑3241926
                                                                                                                                                 |                                                                                                                                                 
aGACCGAAAGCTGCTTTTTGAAGCGACGGTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATTATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTca                                                                                                                                               <  1:1675784/150‑1 (MQ=255)
 gACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTcac                                                                                                                                              >  1:1628571/1‑150 (MQ=255)
 gACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTcac                                                                                                                                              >  2:1388278/1‑150 (MQ=255)
 caCCGAAAGATGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTcac                                                                                                                                              <  2:1608178/149‑1 (MQ=255)
   ccGAAAGCTGCTTTTTGAAGCGACGTTCGTAACGATTGAAATATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACAt                                                                                                                                            <  1:1463389/150‑1 (MQ=255)
           tgctTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGTTTCATAATGTGaagc                                                                                                                                    <  2:798340/150‑3 (MQ=255)
           tgctTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAAc                                                                                                                                    >  2:1359816/1‑150 (MQ=255)
           tgctTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAAc                                                                                                                                    >  2:877966/1‑150 (MQ=255)
           tgctTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAAc                                                                                                                                    <  1:971452/150‑1 (MQ=255)
             ctTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGc                                                                                                                                  <  2:42188/150‑1 (MQ=255)
                tttGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTAt                                                                                                                                         >  2:380092/1‑140 (MQ=255)
                tttGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTAt                                                                                                                                         <  1:380092/140‑1 (MQ=255)
                 ttGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGTGTTTCATAATGTGAAGCGCCGtt                                                                                                                              <  2:600015/150‑1 (MQ=255)
                  tGATGCGACGTTCGTCACGATTGAAATATGTTGTGATACCAATCAGAAAGGCGCTGACACCCACGCCCTGGGCCAGCCAATTCGCGGTCATGATAAATCCTTAGCAGGTATGGACAAGCAAACCGCGTTTCATAATGTGAAGCGCCGttt                                                                                                                             <  1:131813/150‑1 (MQ=255)
                      gCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCTATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTtta                                                                                                                         <  1:62539/150‑1 (MQ=255)
                      gCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTtta                                                                                                                         <  1:1474459/150‑1 (MQ=255)
                       cGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACTCCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGTTTCATAATGTGAAGCGCCGtttgcttt                                                                                                                        <  1:488892/150‑6 (MQ=38)
                           gTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTaac                                                                                                                    <  1:814991/150‑1 (MQ=255)
                              cGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTaacaac                                                                                                                 <  1:449245/150‑1 (MQ=255)
                              cGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTaacaac                                                                                                                 >  2:605026/1‑150 (MQ=255)
                               gTCACGATTGAAAAATGTTGTGATAACAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCAAATACGCGGTCATGATAAATCATTAGCATGTATGGAAAAGCAAACGGCGCTTCACATTATGACACGCCGTATTTTATTAACAACt                                                                                                                >  1:80288/1‑150 (MQ=255)
                                 cACGATTGAAAAATGTTGTGATACCAATCATAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCa                                                                                                              <  1:1547145/150‑1 (MQ=255)
                                   cGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGTGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACATCTCAtt                                                                                                            >  1:715091/1‑150 (MQ=255)
                                   cGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCAtt                                                                                                            >  1:548238/1‑150 (MQ=255)
                                    gATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCAttt                                                                                                           >  1:1054776/1‑150 (MQ=255)
                                        gAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGAc                                                                                                       >  1:256503/1‑150 (MQ=255)
                                        gAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGAc                                                                                                       <  1:1541559/150‑1 (MQ=255)
                                        gAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTATTTTATTAACAACTCATTTCGAc                                                                                                       >  1:727730/1‑150 (MQ=255)
                                         aaaaaTGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACt                                                                                                      >  2:1173081/1‑150 (MQ=255)
                                         aaaaaTGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACt                                                                                                      >  1:1675015/1‑150 (MQ=255)
                                          aaaaTGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACtt                                                                                                     >  2:246810/1‑150 (MQ=255)
                                          aaaaTGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACtt                                                                                                     >  1:401967/1‑150 (MQ=255)
                                          aaaaTGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCCACtt                                                                                                     >  1:728877/1‑150 (MQ=255)
                                              tgttgtGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTtata                                                                                                 >  1:1595888/1‑150 (MQ=255)
                                               gttgtGATACCAATCAGAAAGGCGATGACACCCCCGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAAAGCAGTTTTTTATTAACAACTCATTTCGACTTTATAg                                                                                                >  2:146782/1‑150 (MQ=255)
                                               gttgtGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAg                                                                                                >  2:724984/1‑150 (MQ=255)
                                                ttgtGATACCAATCAGAATGTCGATTACACCCACGCCCTGGGCCATCCAATACGCGGTAATTATAAATCCTTATCATGTATGTAAACGCAAACGGCGCTTCACTTTTTGAAACGCCTTTTTTTATTAACAACTCATTTCGACTTTATAGc                                                                                               <  1:1564959/150‑1 (MQ=255)
                                                 tgtgATACCAATCAGAAAGGCGATGACACCCCCGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCg                                                                                              >  2:1040673/1‑150 (MQ=255)
                                                   tgATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGtt                                                                                            <  2:1430516/150‑1 (MQ=255)
                                                      tACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTAcg                                                                                         >  2:1394423/1‑150 (MQ=255)
                                                       aCCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTAcgc                                                                                        <  2:1470517/150‑1 (MQ=255)
                                                         cAATCAGGAAGGCGATGACACCCACGCCCTGGGCCCGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTGTTTTATTAACACCTCATTGCGACTTTATAGCGGTccgccg                                                                                      >  1:1130387/1‑150 (MQ=255)
                                                           aTCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCt                                                                                    >  1:1515593/1‑150 (MQ=255)
                                                            tCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCtt                                                                                   >  2:428519/1‑150 (MQ=255)
                                                              aGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCtttt                                                                                 <  2:1210899/150‑1 (MQ=255)
                                                               gAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTg                                                                                >  2:502654/1‑150 (MQ=255)
                                                                  aGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAg                                                                             <  2:585300/150‑1 (MQ=255)
                                                                     cGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATATCGTTACGCCGCTTTTGAAGATc                                                                          >  2:1264440/1‑150 (MQ=255)
                                                                     cGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATc                                                                          <  1:546975/150‑1 (MQ=255)
                                                                     cGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATc                                                                          <  2:1054776/150‑1 (MQ=255)
                                                                        tGACACCCACGCCCTGGGCCAGCCAATACGCGGGCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCAcc                                                                       <  1:1131964/150‑1 (MQ=255)
                                                                          acacCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAg                                                                     >  1:1337045/1‑150 (MQ=255)
                                                                           cacCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGATACGCCGCTTTTGAAGATCGCCAGt                                                                    >  1:1160497/1‑150 (MQ=255)
                                                                             cccACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTc                                                                  >  2:887358/1‑150 (MQ=255)
                                                                              ccACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGCTCGCCAGTTcg                                                                 >  2:944459/1‑150 (MQ=255)
                                                                                  gCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGa                                                             <  2:1692035/150‑1 (MQ=255)
                                                                                        ggCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCTCTTTTGAAGATCGCCAGTTCGCGGAAGTCGt                                                       >  2:1668951/1‑150 (MQ=255)
                                                                                        ggCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGt                                                       >  1:1508724/1‑150 (MQ=255)
                                                                                         gccagccaATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGtt                                                      <  2:1337045/150‑1 (MQ=255)
                                                                                          ccagccaATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTAc                                                    >  1:1612428/1‑150 (MQ=255)
                                                                                             gccaATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTAc                                                                                          >  2:370076/1‑110 (MQ=255)
                                                                                             gccaATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTAc                                                                                          <  1:370076/110‑1 (MQ=255)
                                                                                             gccaATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTAAGCCGCTTTTTAAGATCGCCAGTTCGCGGAAGTCGTTAAGc                                                  >  1:1518217/1‑150 (MQ=255)
                                                                                              ccaATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCt                                                 <  1:1303293/150‑1 (MQ=255)
                                                                                              ccaATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCAAGTTCGCGGAAGTCGTTACGCt                                                 >  1:1098540/1‑150 (MQ=255)
                                                                                              ccaATAAGCGGTCATGATAAATCCTTAGCAGGTATGGAAAATCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCt                                                 <  2:1148865/150‑1 (MQ=255)
                                                                                               caATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTc                                                <  1:1173081/150‑1 (MQ=255)
                                                                                                         tCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGtt                                      <  2:427519/150‑1 (MQ=255)
                                                                                                          cATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGttt                                     <  1:895066/150‑1 (MQ=255)
                                                                                                            tGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGc                                   >  2:781564/1‑150 (MQ=255)
                                                                                                            tGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGc                                   >  2:1083519/1‑150 (MQ=255)
                                                                                                             gATAAATCCTTATCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGATTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCt                                  <  2:502130/150‑1 (MQ=255)
                                                                                                             gATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCt                                  <  2:1312029/150‑1 (MQ=255)
                                                                                                              aTAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCtt                                 <  1:605026/150‑1 (MQ=255)
                                                                                                                aaaTCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTAc                               >  2:1200322/1‑150 (MQ=255)
                                                                                                                 aaTCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTAcc                              <  1:963814/150‑1 (MQ=255)
                                                                                                                    ccTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGtt                           <  1:1424101/150‑1 (MQ=255)
                                                                                                                     cTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTg                          <  1:371929/150‑1 (MQ=255)
                                                                                                                     cTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTg                          <  2:148682/150‑1 (MQ=255)
                                                                                                                     cTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGATTACCGTTg                          >  2:1575816/1‑150 (MQ=255)
                                                                                                                      ttAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTgg                         <  2:1100340/150‑1 (MQ=255)
                                                                                                                      ttAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTgg                         <  1:1394423/150‑1 (MQ=255)
                                                                                                                      ttAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTgg                         <  2:574373/150‑1 (MQ=255)
                                                                                                                          cAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCaaa                     >  2:927963/1‑150 (MQ=255)
                                                                                                                           aGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAAc                    <  2:548238/150‑1 (MQ=255)
                                                                                                                            ggTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACt                   >  2:1186632/1‑150 (MQ=255)
                                                                                                                             gTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTc                  <  2:1628571/150‑1 (MQ=255)
                                                                                                                              tATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGATACAGGTTTGCCAACGGTAAGCAAAcctg                 <  2:1199874/150‑4 (MQ=255)
                                                                                                                                 ggAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACg              >  1:55874/1‑150 (MQ=255)
                                                                                                                                 ggAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACg              >  1:1587471/1‑150 (MQ=255)
                                                                                                                                  gAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGa             >  2:1184422/1‑150 (MQ=255)
                                                                                                                                   aataaaaaaCGGCGTTTCATAATGTGAAGCGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGAt            >  2:697671/7‑150 (MQ=39)
                                                                                                                                   aaaaGCAAATGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGAt            >  2:195003/1‑150 (MQ=255)
                                                                                                                                   aaaaGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCTCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGAt            >  1:1514662/1‑150 (MQ=255)
                                                                                                                                      aGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGgt         >  2:1096327/1‑150 (MQ=255)
                                                                                                                                       gCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTAGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGgtg        <  2:256503/150‑1 (MQ=255)
                                                                                                                                         aaaCGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCTCCAGTGCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTc      <  1:1135419/150‑1 (MQ=255)
                                                                                                                                         aaaCGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTc      >  2:1513413/1‑150 (MQ=255)
                                                                                                                                          aaCGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGAGCGCCAGTTCGCGGAAGTCGTTACGCGCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCg     <  2:1323279/150‑1 (MQ=255)
                                                                                                                                            cGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGAt   <  1:762053/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCGGTCATGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTCACATTATGAAACGCCGTTTTTTATTAACAACTCATTTCGACTTTATAGCGTTACGCCGCTTTTGAAGATCGCCAGTTCGCGGAAGTCGTTACGCTCGTTACAGGTTTGCTTACCGTTGGCAAACTCAACGATGGTGTCGATA  >  NC_000913/3241636‑3241926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: