Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,259,122 T→G 5.2% E176D (GAA→GAC tdcG ← L‑serine dehydratase 3, anaerobic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,259,1220TG 5.2% 331.6 / 5.9 121E176D (GAA→GACtdcGL‑serine dehydratase 3, anaerobic
Reads supporting (aligned to +/- strand):  ref base T (51/61);  new base G (3/4);  total (55/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

Only 100 of 124 total aligned reads displayed.

GTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCAT  >  NC_000913/3258973‑3259271
                                                                                                                                                     |                                                                                                                                                     
gtgtTCATCGCACGTTCAATACCGTCTTGCATCACTTGCCAGATACGGGCAATAGCGGCGTCATTTTGCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGTCCAGATATAGACATGCCGTTGTAATCACACATTTTCAGCAGt                                                                                                                                                       <  1:1613853/150‑1 (MQ=255)
  gtTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTAGTGCATCATCAGACCAGATATAGACAGGCCGTTGAAATCACACATTTTCAGCAGtta                                                                                                                                                     >  2:359380/1‑149 (MQ=255)
       tCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACctg                                                                                                                                                >  2:346781/1‑150 (MQ=255)
       tCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACctg                                                                                                                                                <  2:1078230/150‑1 (MQ=255)
        cccACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACctgc                                                                                                                                               >  1:579108/1‑150 (MQ=255)
          cACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACctgctg                                                                                                                                             <  2:890566/150‑1 (MQ=255)
          cACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACctgctg                                                                                                                                             >  1:160697/1‑150 (MQ=255)
          cACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGGTGAActgctg                                                                                                                                             <  2:890666/150‑1 (MQ=255)
            cGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGGTGAACTGCTGaa                                                                                                                                           <  2:828527/150‑2 (MQ=255)
             gTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGt                                                                                                                                          >  2:758258/1‑150 (MQ=255)
              ttCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTg                                                                                                                                         <  2:508056/150‑1 (MQ=255)
               tCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTgg                                                                                                                                        >  2:1206596/1‑150 (MQ=255)
               tCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGGTGAACTGCTGaaaat                                                                                                                                        <  1:798353/150‑5 (MQ=255)
                  aTACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGaaa                                                                                                                                     >  1:1613238/1‑150 (MQ=255)
                     ccGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCg                                                                                                                                  >  1:656498/1‑150 (MQ=255)
                     ccGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCg                                                                                                                                  >  2:308172/1‑150 (MQ=255)
                     ccGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCg                                                                                                                                  <  1:1509725/150‑1 (MQ=255)
                       gTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTa                                                                                                                                >  1:5078/1‑150 (MQ=255)
                         cATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAg                                                                                                                              <  1:620326/150‑1 (MQ=255)
                         cATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAg                                                                                                                              <  1:1221446/150‑1 (MQ=255)
                         cATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATAAGACCAGATAAAGACAGGCCCTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAg                                                                                                                              >  2:1570662/1‑150 (MQ=255)
                           tGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGt                                                                                                                            >  2:1333877/1‑150 (MQ=255)
                           tGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAAGCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGt                                                                                                                            >  1:147157/1‑150 (MQ=255)
                             cATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTAc                                                                                                                          <  2:1578430/150‑1 (MQ=255)
                                    tGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGtt                                                                                                                   >  2:491270/1‑150 (MQ=255)
                                     gCCAGATACGGGCAAAAGCTGCGTCATTTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGGATCTTCAGACCAGATATTGACAGGCCGTTGTAATCACAGATTTTCTGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGttt                                                                                                                  <  2:793225/150‑1 (MQ=255)
                                       ccgctACGGGCAAAACCGGCTGTAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTTAGGTACGGACGTTTCg                                                                                                                <  2:1059579/146‑1 (MQ=255)
                                       cAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCg                                                                                                                <  1:940003/150‑1 (MQ=255)
                                       cAGATACGGGCAAAACCGGCGGCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCg                                                                                                                <  2:152625/150‑1 (MQ=255)
                                        aGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACTGACGTTTCGa                                                                                                               >  2:638995/1‑150 (MQ=255)
                                         gATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGAc                                                                                                              >  1:912955/1‑150 (MQ=255)
                                         gATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGAc                                                                                                              <  2:649622/150‑1 (MQ=255)
                                               ggCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTg                                                                                                        >  1:47495/1‑150 (MQ=255)
                                                    aaCCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACa                                                                                                   <  1:935803/150‑1 (MQ=255)
                                                           gTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGaa                                                                                            >  2:1622493/1‑150 (MQ=255)
                                                                  tCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGttct                                                                                     <  2:1074886/150‑1 (MQ=255)
                                                                  tCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGttct                                                                                     <  1:1405285/150‑1 (MQ=255)
                                                                  tCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGttct                                                                                     <  2:1611862/150‑1 (MQ=255)
                                                                  tCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGttct                                                                                     <  2:1710025/150‑1 (MQ=255)
                                                                     gCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGttcttct                                                                                  <  2:1638900/150‑1 (MQ=255)
                                                                     gCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGttcttct                                                                                  <  1:247372/150‑1 (MQ=255)
                                                                       tttGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGttcttcttc                                                                                <  2:1017732/150‑1 (MQ=255)
                                                                       tttGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGttcttcttc                                                                                <  1:699063/150‑1 (MQ=255)
                                                                        ttGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCg                                                                               <  2:421091/150‑1 (MQ=255)
                                                                        ttGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCATAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTACg                                                                               >  2:1536009/1‑150 (MQ=255)
                                                                         tgctgcGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGa                                                                              >  2:734690/1‑150 (MQ=255)
                                                                            tgcGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCTGAAGTGTTCTTCTTCGACaa                                                                           <  2:463997/150‑1 (MQ=255)
                                                                             gcgcAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAAt                                                                          <  1:631598/150‑1 (MQ=255)
                                                                                cAGCGCTAGCTCGTTGTGCATCATCAGCCCAGATATAGACAGGCCGTTGTAATCACAGATTTTCAGCAGGTCACCGGCTGAGTGGAACTCGTAAGGTGCGGCCGTTTCGCCATGGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATaac                                                                       >  2:270255/1‑149 (MQ=255)
                                                                                  gcgcTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCAACTGCTGAGTGGAAATCGTAAGGTACGGACGTATCGACATCGTGCGACAGGACGAAGTGTTCTTCTTCGACAATAAAcc                                                                     >  1:1556111/1‑150 (MQ=255)
                                                                                   cgcTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGATTCGACATCGTGCGAAAGGCCGAAGTGTTCTTCTTCGACAATAAAccc                                                                    >  2:156036/1‑150 (MQ=255)
                                                                                     cTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACccgc                                                                  <  2:848621/150‑1 (MQ=255)
                                                                                        gCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACccgccgc                                                               >  1:150573/1‑150 (MQ=255)
                                                                                          tCGTTGTGCATCATCAGACCAGATATAGACAGGCCGGTGTAATCACACATTTTCCGCAGATCACCTGCTGAGTGGAAATCGTCAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACccgccgccg                                                             <  1:107736/150‑1 (MQ=255)
                                                                                           cGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGa                                                            <  1:1120822/150‑1 (MQ=255)
                                                                                                 gCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGt                                                      <  2:324147/150‑1 (MQ=255)
                                                                                                     catcaGCCCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTAACCTGCTGAGTGGAAATAGTAAGGTACGGACGTTTCGACATCGTGCGACACGCCGAAGTGTTGTTCTTCGACAATACACCCGCCGCCGACAGAGTAAtt                                                  >  2:822640/1‑149 (MQ=255)
                                                                                                     catcaGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGttcttcttc                                                                                >  2:1468941/1‑120 (MQ=255)
                                                                                                      atcaGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAg                                                 <  1:592286/150‑1 (MQ=255)
                                                                                                       tcaGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAgg                                                >  2:709944/1‑150 (MQ=255)
                                                                                                         aGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGtt                                              <  2:719921/150‑1 (MQ=255)
                                                                                                          gACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACTGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCTACAGAGTAATAGGttt                                             >  1:1628503/1‑150 (MQ=255)
                                                                                                          gACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGttt                                             >  1:1681417/1‑150 (MQ=255)
                                                                                                            ccAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTa                                           >  2:1439716/1‑150 (MQ=255)
                                                                                                            ccAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTa                                           >  2:1143558/1‑150 (MQ=255)
                                                                                                            ccAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTa                                           >  2:1137730/1‑150 (MQ=255)
                                                                                                             cAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTAc                                          <  1:1397961/150‑1 (MQ=255)
                                                                                                               gATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACtt                                        <  2:579108/150‑1 (MQ=255)
                                                                                                                  ataGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAAt                                     >  1:1299596/1‑150 (MQ=255)
                                                                                                                  ataGACAGGCCGTTGTAATCACAAATTTTCAGCAGTTCACCTGCTGAGTGTAAATCGTAAGGTAAGGACGTTGCGACATAGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAAt                                     <  1:823577/150‑1 (MQ=255)
                                                                                                                   taGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATa                                    <  1:1448150/150‑1 (MQ=255)
                                                                                                                   taGACAGGCCGTTGTAATCACACATTATCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATa                                    <  2:1354196/150‑1 (MQ=255)
                                                                                                                     gACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGc                                  <  1:758258/150‑1 (MQ=255)
                                                                                                                           ccGTTGTAATCACACATTTTCAGCAGTTCGCCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTcc                            <  1:338293/150‑1 (MQ=255)
                                                                                                                            cGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCt                           <  2:902484/150‑1 (MQ=255)
                                                                                                                             gTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTg                          >  2:698773/1‑150 (MQ=255)
                                                                                                                               tGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTc                        <  1:346781/150‑1 (MQ=255)
                                                                                                                                  aaTCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCt                     <  2:642779/150‑1 (MQ=255)
                                                                                                                                   aTCACACCTTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGGGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCtt                    <  2:369535/150‑1 (MQ=255)
                                                                                                                                   aTCACACAGTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGATGCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCtt                    <  2:1133132/150‑1 (MQ=255)
                                                                                                                                      acacaTTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCa                 <  1:1137730/150‑1 (MQ=255)
                                                                                                                                      acacaTTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCa                 <  1:1143558/150‑1 (MQ=255)
                                                                                                                                      acacaTTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCa                 <  1:1439716/150‑1 (MQ=255)
                                                                                                                                      acacaTTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTATTCCTGTCCCTTCCa                 >  2:442650/1‑150 (MQ=255)
                                                                                                                                        ttccactCAGCAGGTGAACTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCAGCCGCCGACAGAGTAATAGGTGTTACTTAATAGCTCTTCCTGTCCCTTCCAgg               >  1:1062015/7‑150 (MQ=255)
                                                                                                                                        acaTTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAgg               <  2:419950/150‑1 (MQ=255)
                                                                                                                                         caTTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGc              >  2:1569980/1‑150 (MQ=255)
                                                                                                                                           ttttCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAATCCCGCCGCCGACTGAGTAATAGGTTTTAATTAATAGCTCTTCCTGTCCCTTCCAGGCAg            >  1:252597/1‑150 (MQ=255)
                                                                                                                                           ttttCAGCAGTTCACCTGCTGAGTGGAAATAGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAAGAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAg            >  1:256258/1‑150 (MQ=255)
                                                                                                                                            tttCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGt           <  1:67450/150‑1 (MQ=255)
                                                                                                                                             ttCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTg          >  2:1380756/1‑150 (MQ=255)
                                                                                                                                               cagcagTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGAt        >  1:838714/1‑150 (MQ=255)
                                                                                                                                                  cagTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATcca     <  1:1206596/150‑2 (MQ=255)
                                                                                                                                                  cagTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCg     <  1:601863/150‑1 (MQ=255)
                                                                                                                                                  cagTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCg     >  1:866640/1‑150 (MQ=255)
                                                                                                                                                  cagTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCg     <  2:108525/150‑1 (MQ=255)
                                                                                                                                                   cgTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACCTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACATTAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGc    <  2:331677/149‑1 (MQ=255)
                                                                                                                                                   agTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGc    >  1:1299047/1‑150 (MQ=255)
                                                                                                                                                    gTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCa   >  1:533222/1‑150 (MQ=255)
                                                                                                                                                     ttCACCTGCTGAGTGGAAATCGTCAGGGACGGACGTTTCGACATCGTGCGACTGGCCGAAGTGTACTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTGTACTTAATAGCTCTACCTGGCCCTTCCAGGCAGTGAGCAGCAt  <  2:1313397/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTGTTCATCCCACGTTCAATACCGTCATGCATCACTTGCCAGATACGGGCAAAACCGGCGTCAATTTCCGCTTTGCTGCGCAGCGCTAGCTCGTTGTGCATCATCAGACCAGATATAGACAGGCCGTTGTAATCACACATTTTCAGCAGTTCACCTGCTGAGTGGAAATCGTAAGGTACGGACGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCAT  >  NC_000913/3258973‑3259271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: