Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,263,501 G→C 6.7% P54A (CCG→GCG)  tdcD ← propionate kinase/acetate kinase C, anaerobic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,263,5010GC 6.7% 338.6 / 12.2 115P54A (CCG→GCG) tdcDpropionate kinase/acetate kinase C, anaerobic
Reads supporting (aligned to +/- strand):  ref base G (40/67);  new base C (2/6);  total (42/73)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-02

Only 100 of 115 total aligned reads displayed.

CATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCG  >  NC_000913/3263354‑3263647
                                                                                                                                                   |                                                                                                                                                   
cATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTg                                                                                                                                                   >  2:1180272/1‑150 (MQ=255)
  tCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCt                                                                                                                                                 <  1:1715302/150‑1 (MQ=255)
  tCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCt                                                                                                                                                 <  2:522346/150‑1 (MQ=255)
   cGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGTCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTg                                                                                                                                                >  1:1718385/1‑150 (MQ=255)
   cGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTg                                                                                                                                                <  2:927902/150‑1 (MQ=255)
   cGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTg                                                                                                                                                <  1:1664951/150‑1 (MQ=255)
   cGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTg                                                                                                                                                >  2:1134400/1‑150 (MQ=255)
     gttataatGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCTTTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGc                                                                                                                                              <  1:1012071/147‑1 (MQ=255)
     gTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGc                                                                                                                                              <  1:1545392/150‑1 (MQ=255)
       aataatGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGAACCTTCGTAGCTGTGGTGAGCCAGCGGggctggcta                                                                                                                                            >  2:1282847/1‑149 (MQ=255)
        ataatGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCCGCGCGGCTgggggt                                                                                                                                           >  2:1390433/1‑146 (MQ=255)
        ataatGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCACCGCTGGctca                                                                                                                                           <  1:1187134/150‑2 (MQ=255)
        ataatGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCACCGCTGGctca                                                                                                                                           <  1:1142639/150‑2 (MQ=255)
         taatGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCACCGCTGGctcac                                                                                                                                          <  1:990778/150‑3 (MQ=255)
           atGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTccc                                                                                                                                       <  1:353513/150‑1 (MQ=255)
           atGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTccc                                                                                                                                       <  1:1677319/150‑1 (MQ=255)
           atGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGAGAGCCAGCACCGCTGGCTCAcca                                                                                                                                       <  1:197538/150‑2 (MQ=255)
              gCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCa                                                                                                                                    <  2:76209/150‑1 (MQ=255)
              gCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCGGGCGCTcccccc                                                                                                                                    >  2:1644321/1‑149 (MQ=255)
                   cTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGCGCCAGCGGTGCTGGCTCTCCCCCATTTAc                                                                                                                               >  1:38335/1‑150 (MQ=255)
                   cTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGCGCCAGCGGTGCTGGCTCGCCCCCATTTAc                                                                                                                               >  2:222611/1‑150 (MQ=255)
                         aaaaaTACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTTCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAACGGTTCAGGATCTACCCCCATTAAGGATaa                                                                                                                         >  2:1450045/1‑150 (MQ=255)
                              tACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATg                                                                                                                    <  1:1639534/150‑1 (MQ=255)
                              tACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATg                                                                                                                    <  1:1439578/150‑1 (MQ=255)
                               aCTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGc                                                                                                                   >  2:33474/1‑150 (MQ=255)
                                cTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCa                                                                                                                  <  1:730108/150‑1 (MQ=255)
                                  gccgccGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTTCGGATAAGAATGCAtt                                                                                                                >  2:1711774/1‑150 (MQ=255)
                                           aGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGGGCCCGCGGTGCGGGCTCGCACCCATTTAACGATAAGCAAGCATTTTACGAggt                                                                                                       >  2:867431/1‑148 (MQ=255)
                                           aGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGGATTTTGCGAGtt                                                                                                       >  2:744089/1‑150 (MQ=255)
                                            tcGATTCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTTTGGTGAGCCAGCGGTGCTGGCTCTCACCCATTTACGGATAAGAATGCATTTTCCTAGTTa                                                                                                      <  2:1520293/149‑1 (MQ=255)
                                             cGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGCGCCAGCGGTGCTGGGTCTCCCCCCTTTAAGGATAAGAATGCATTTTACGAGttta                                                                                                     >  1:507688/1‑148 (MQ=255)
                                                    gTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTc                                                                                              <  2:1455464/150‑1 (MQ=255)
                                                    gTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCGCGCCCCCAGTTTCGGTTAATTATTCATTTTGCGAGTTTATAACGtg                                                                                              >  1:1046188/1‑149 (MQ=255)
                                                      ggCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCgg                                                                                            >  1:895427/1‑150 (MQ=255)
                                                       gCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGc                                                                                           >  1:1026193/1‑150 (MQ=255)
                                                        ccAATTAAGGCCACCCTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCATTTGCCTTCAATGCACCTTCGTAGCTGTGGTGCGCCCGAGGTGGGTGGGCTCGCCCATTTAAGGGTTATAATGGATTTTCCGAGTTATTACCgggcggc                                                                                          >  2:1599870/1‑144 (MQ=255)
                                                         cAATTAAGGCCACACTGTCATTTAATTTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCaa                                                                                         <  1:1500854/150‑1 (MQ=255)
                                                            ttAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGCGCCAGCGGGGCTGGCGCTCCCCCAATTACGGATTATAATGCAATTTCCCAGTTAATAACGTCGGCAATAc                                                                                      >  2:515358/1‑150 (MQ=255)
                                                             tAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATAcc                                                                                     <  2:453919/150‑1 (MQ=255)
                                                             tAAGGCCACACTGTAATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATAcc                                                                                     <  2:1225608/150‑1 (MQ=255)
                                                               aGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTg                                                                                   <  1:1188849/150‑1 (MQ=255)
                                                               aGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTg                                                                                   <  1:1181975/150‑1 (MQ=255)
                                                                gtccACACTGGCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAACGCACCTTCGTAGCTGTGGGGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGa                                                                                  <  1:80737/148‑1 (MQ=255)
                                                                 gCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCCGCGGGGGGGGGGGTACCCCCATTTAGGGTAATAATTGATTTTACCAGTTAATAACGTCGGGAATAACTGAc                                                                                 >  1:1351802/1‑150 (MQ=255)
                                                                 gCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGAc                                                                                 <  2:596967/150‑1 (MQ=255)
                                                                  ccACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATAACGTCGGCAATACCTGACa                                                                                >  2:253064/1‑150 (MQ=255)
                                                                    acacTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACAtt                                                                              <  1:20415/150‑1 (MQ=255)
                                                                    acacTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACAtt                                                                              <  1:25781/150‑1 (MQ=255)
                                                                        tGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATa                                                                          <  2:325802/150‑1 (MQ=255)
                                                                         gTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATAc                                                                         <  1:650114/150‑1 (MQ=255)
                                                                         gTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATAc                                                                         <  1:1328707/150‑1 (MQ=255)
                                                                         gTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCCGTGCGGGCTCTACCCCATTTAAGGATAATAAAGCATTTTACGAGGTAATACCGTCGGGAATAACAGACAATtataa                                                                         >  1:422694/1‑149 (MQ=255)
                                                                            aTTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGGGCCAGCGGTGCTGGCTCTCCCCCAATTAAGGATAATAATGGATTTTGCGAGTTAATAACGTCGGCAATAACAGACAATAATAcata                                                                      >  1:1717984/1‑147 (MQ=255)
                                                                              ttAAATTCCGTTTTTCCATTTCAAATGCAATTGCCTTCAATGCACCTTAGTAGCTGTGGTGAGACAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTcac                                                                    <  1:1068046/150‑1 (MQ=255)
                                                                              ttAAATTCCGTTTTTCCAGTTCAATTGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTcac                                                                    <  1:1062492/150‑1 (MQ=255)
                                                                                 aaTTCCGTTTTTCCAGTTCAAATTCTATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGt                                                                 <  1:1450045/150‑1 (MQ=255)
                                                                                  aTTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGCGCCAGCGGTGCTGGCTGTCCCCCATTTAAGGATAAGAATGCAATTTACGAGTTAAAACCCTCGGCAAAAACAGACATTAATACTTCACAGtg                                                                >  2:456316/1‑149 (MQ=255)
                                                                                  aTTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTc                                                                <  2:712961/150‑1 (MQ=255)
                                                                                    tCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTACCCCATTTACGGATAAGAATGCATTTTACGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGc                                                              >  1:1038697/1‑150 (MQ=255)
                                                                                     ccGTTTTTCCAGTTCAAATGAAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCATCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCt                                                             <  2:790544/150‑1 (MQ=255)
                                                                                       gTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTgg                                                           <  1:825732/150‑1 (MQ=255)
                                                                                         ttttCCAGTTCAAATGCATTTGCCTTCAATTCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCa                                                         <  1:548906/150‑1 (MQ=255)
                                                                                           ttCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTAAGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCCCAGTCGCGGGCagc                                                       >  1:1027097/1‑148 (MQ=255)
                                                                                            tCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCg                                                      <  1:1362869/150‑1 (MQ=255)
                                                                                            tCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCg                                                      <  2:895427/150‑1 (MQ=255)
                                                                                            tCCAGTTAAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCg                                                      <  2:362014/150‑1 (MQ=255)
                                                                                              cAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAg                                                    <  2:1250517/150‑1 (MQ=255)
                                                                                               aGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGc                                                   <  2:53966/150‑1 (MQ=255)
                                                                                               aGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGc                                                   <  1:602335/150‑1 (MQ=255)
                                                                                                  tCAAATGCAATTTCCTTCAATGCAACTTAGTATCTGTGGTGATCCAGCGGTTCTTGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACg                                                <  1:1197071/150‑1 (MQ=255)
                                                                                                     aaTGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGCTCCAGCGGTGCTGGCTCACCCCCATTTACGGATAAGAATCCATTTTGCGAGTTTATACCGTCGGCAATACCGGACAGTAATACTTACCAGGCGCAGGCATCGAGCACGGaa                                             >  2:74292/1‑150 (MQ=255)
                                                                                                       tGCAATTGCCTTCAATGCACCTTCGTAGCTCTGGTGAGCCAGCGGGGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGaaaa                                           <  2:199448/150‑1 (MQ=255)
                                                                                                        gCAATTGCCTTCAATTCACCTTCGTAGCTGTGGGGATGCAGCGGTGCGGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGGTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAAc                                          <  2:880591/150‑1 (MQ=255)
                                                                                                          aaTTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGAATTTTCCGAGTAAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACtt                                        <  2:1214722/150‑1 (MQ=255)
                                                                                                            ttGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTaa                                      <  1:706600/150‑1 (MQ=255)
                                                                                                            ttGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTaa                                      <  2:1528017/150‑1 (MQ=255)
                                                                                                              gCCTTCAATGCACATTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCACAACCCCATTTACGTATAATAATGAATTTTACGAGTTAATACCCACCGGAATAACAGACATTAATACATTACAGTCGCTGGCAAACAGCACGGAAAACATAATc                                    >  1:914325/1‑150 (MQ=255)
                                                                                                               ccTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTGAATCg                                   <  2:1693500/150‑1 (MQ=255)
                                                                                                                cTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTAAGGATAAGAATTCATTTTGCGAGTTAATAACGTCGGCAATACAAGACATTAATACTTCACAGTCGCTGGCATAGAGCACGGAAAACTTaaaaga                                  >  2:406218/1‑146 (MQ=255)
                                                                                                                 ttCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGaa                                 <  1:810908/150‑1 (MQ=255)
                                                                                                                     aTGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATTCCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACg                             <  2:1046188/150‑1 (MQ=255)
                                                                                                                         aCCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTTCGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAAcc                         <  1:1337489/150‑1 (MQ=255)
                                                                                                                          ccTTCGTAGCTGTGGTGCGCCAGCGGTGCTGGCTCTACCCCATTTAAGGATAAGAATGCATTTTACGAGTTAATACCGTCGGCAATAACAGACAATAATAATTAACAGTAGCTGGCCTCGAGCACCGAAAACTTAATAGAATACGAAccc                        >  2:1634237/1‑149 (MQ=255)
                                                                                                                          ccTTCGTAGCTGTGGTGAGCCCGCGGTGCTGGCTGGCCCCCAATTACGGATAAGAAATCATTTTGCGAGTTAAAAACGTCGGCAAAACCAGACATTTAAACATAACAGTAGCGTGCCTAGAGCCCGGAAAAATTAAAATAAGaccaacca                        >  2:479953/1‑150 (MQ=255)
                                                                                                                          ccTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTAGACCCCAATTAAGGATAAGAAAGCATTTTACCAGTTAAAAACGTCGGCAATACCGGACAATAATAATTCACAGTCGCAGGCATAGAGCACGGAAAACATAATCGAAAACGAACca                        >  2:1601709/1‑150 (MQ=255)
                                                                                                                           cTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACcac                       <  1:1657323/150‑1 (MQ=255)
                                                                                                                              cGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGt                    <  1:785139/150‑1 (MQ=255)
                                                                                                                               gTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGtt                   <  2:1516315/150‑1 (MQ=255)
                                                                                                                                tAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTta                  <  1:1511890/150‑1 (MQ=255)
                                                                                                                                tAGCTGTGGGGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTta                  <  2:1237003/150‑1 (MQ=255)
                                                                                                                                   cTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTtaata               <  1:268597/150‑1 (MQ=255)
                                                                                                                                   cTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTtaata               <  2:1634904/150‑1 (MQ=255)
                                                                                                                                   cTGTGGGGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTtaata               <  1:925614/150‑1 (MQ=255)
                                                                                                                                      gggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAAcc            <  2:588628/149‑1 (MQ=255)
                                                                                                                                      gggagAGCCAGCACCGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAAcc            >  2:1178747/5‑150 (MQ=255)
                                                                                                                                        gtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCaa          <  1:1309151/150‑1 (MQ=255)
                                                                                                                                         tgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCaaa         >  1:1366031/1‑150 (MQ=255)
                                                                                                                                           aGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAaac       <  2:89366/150‑1 (MQ=255)
                                                                                                                                               aGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAAcc   >  2:889012/1‑150 (MQ=255)
                                                                                                                                                gCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCg  <  1:814668/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCG  >  NC_000913/3263354‑3263647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: