Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,269,956 T→A 13.5% intergenic (+354/+260) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,269,9560TA13.5% 114.5 / 2.6 62intergenic (+354/+260)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base T (4/47);  new base A (5/3);  total (9/53)
Fisher's exact test for biased strand distribution p-value = 1.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAG  >  NC_000913/3269810‑3270104
                                                                                                                                                  |                                                                                                                                                    
cgcATCGGGTATGAATAAAGCGCACTCAAAAATCTTAAACCCGCCGTATCGGTTTTTTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaa                                                                                                                                                   <  2:30562/150‑1 (MQ=17)
cgcATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTaaa                                                                                                                                                   <  1:1210993/150‑1 (MQ=255)
cgcATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTaaa                                                                                                                                                   <  1:1424270/150‑1 (MQ=255)
   aTCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGATTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCAGCCCCTCCAAAAGTAAAAAc                                                                                                                                                <  1:1046247/150‑1 (MQ=255)
    tgcgtcATGAATAAAGCGCACTCAACAATTTTAAACCCGCCGGAACGGGTTATTACGTAAATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAcc                                                                                                                                               <  2:1115069/145‑1 (MQ=17)
    tCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAAcc                                                                                                                                               <  2:296861/150‑1 (MQ=255)
      cGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTAATTCATCTTTCTGCCCCACCAAAAGAAAAAAcccc                                                                                                                                             >  2:1376022/1‑149 (MQ=255)
       ggCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATCTTTCTGCCCCTACAAAAGTAAAAACccgc                                                                                                                                            >  1:862601/1‑150 (MQ=255)
         cATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACccgccg                                                                                                                                          <  2:777270/150‑1 (MQ=255)
         cATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAAAGTCATTAATATTTATGCCCCTACAAAAGAAAAAAccccccc                                                                                                                                          >  2:1728162/1‑149 (MQ=21)
           tGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTACATAAGTAAAAAccccgcgat                                                                                                                                        >  1:404133/1‑144 (MQ=255)
             aaTAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAACCCGCCGAAGCg                                                                                                                                     <  2:533927/150‑1 (MQ=255)
              aTAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGTGTCGTGGACAGGCATTCATCATTCTGCCCCACCAAAAGAAAAAACCAGCCGAAGCg                                                                                                                                     >  1:63304/1‑150 (MQ=18)
                aaaGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCggg                                                                                                                                   <  1:1292738/150‑1 (MQ=255)
                aaaGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCggg                                                                                                                                   <  1:237941/150‑1 (MQ=255)
                 aaGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGAGt                                                                                                                                  >  1:887914/1‑150 (MQ=255)
                    cgcACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCTTCGGCGGGtttt                                                                                                                               <  2:1057292/150‑1 (MQ=255)
                    cgcACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCTTCGGCGGGtttt                                                                                                                               <  2:651453/150‑1 (MQ=255)
                       aCTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                                                            <  1:685851/150‑1 (MQ=255)
                        cTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCTTCGGCGGGTTTTTAct                                                                                                                           <  2:1136857/150‑2 (MQ=255)
                        cTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACg                                                                                                                           <  1:648016/150‑1 (MQ=255)
                        cTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACAGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACg                                                                                                                           <  1:468677/150‑1 (MQ=255)
                            acaaTCTGAAACCCTGCGGAACGGTTTATTACGTACATGAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAATAGTAAAAACCCGCTTCGGCGGGTTTTTActtttg                                                                                                                       <  2:421175/150‑6 (MQ=17)
                                 cTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGTGTCGTGGGCAAGAATTCAACTTTGATCGCCTCCCAAAGAGAAAACACGCCGACGCGGGGTTTTTAGTAAAAAAgg                                                                                                                  >  1:1326909/1‑150 (MQ=17)
                                  tGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGt                                                                                                                 <  1:1562999/150‑1 (MQ=255)
                                  tGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGt                                                                                                                 <  1:1519330/150‑1 (MQ=255)
                                   gAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTg                                                                                                                <  1:73856/150‑1 (MQ=255)
                                    aaaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGa                                                                                                               <  1:1275831/150‑1 (MQ=255)
                                     aaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGaa                                                                                                              <  1:1343546/150‑1 (MQ=255)
                                            cgtaaCGGTTTTTTACTTATATCAGGTAAAACTCACCTATATGCCGCTTTCTTTTGTGTATATTGTCGTCGTCAGACTTTTTTATTTCTGGCCCTCCAAAAGCTTAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAcc                                                                                                       <  2:222606/147‑1 (MQ=2)
                                            cgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAcc                                                                                                       <  2:1633467/150‑1 (MQ=255)
                                             ggAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCg                                                                                                      <  2:1300624/150‑1 (MQ=255)
                                              gAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGa                                                                                                     <  2:1553651/150‑1 (MQ=255)
                                                aCGGTTTATTACGTACATCAGGTAAAACTGACCGATAATCCGCTTTCTTTTGTGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATa                                                                                                   <  2:1446330/150‑1 (MQ=255)
                                                aCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTAGGGTATAGGGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATa                                                                                                   <  2:305646/150‑1 (MQ=255)
                                                     ttattaCGGACATCATTTAAAACTGACCGATTAGCCGGTTTCTTTTGGGTATTGTGTCGTGGACAGTCATTCTTCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGTCCGATAAGCCg                                                                                              <  2:72061/150‑1 (MQ=14)
                                                       attaCGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGct                                                                                            <  1:967177/150‑1 (MQ=255)
                                                        ttaCGTACATCATTTAAAACTTACCGATAAGCCGCTTTCTTTTTAGGTTAGTGTCGTCGACAGTGTTTCATCTTTATCCCACTCCAAAAGTAAAAACCCGCCGTATCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctt                                                                                           <  2:926396/150‑1 (MQ=14)
                                                          acttACATCAGGTAAAACTGACCGATAAGCCTCTTTCTTTTTGGTTTAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttc                                                                                         <  2:1496457/147‑1 (MQ=255)
                                                          aCGTACATCAGGTAAAACTGACCGATAAGCCGCGATCTTTTGGTTTTAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTATAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttc                                                                                         <  2:1489683/150‑1 (MQ=14)
                                                            gTACATCATGTAAAACTGAGCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttctt                                                                                       <  1:363949/150‑1 (MQ=255)
                                                             tACATCATTTAAAACTTACCGATAAGCCGCTTTCATTTGTGTATAGTGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGctttcttt                                                                                      <  1:994560/150‑1 (MQ=11)
                                                               aattaGGCAAAACTCACCGATTCGCCTCTTGCTTTAGTCTTTTGTGTCGTCGCCTGTGTTTTTTATTTCTGCCCCTCAAAAATTTTAAACCCGCCGAACCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                    <  2:1155222/146‑1 (MQ=2)
                                                                 tCATGTAAATCTGACCGATAAGCCGCTTTATTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTggg                                                                                  <  1:1565562/150‑1 (MQ=255)
                                                                 tCAGGTAACACTGACCGATAAGCGGGTTTCTTTTGTGTTTAGTGTCGTGGACAGTCATTCATCTTTATTCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTggg                                                                                  <  1:467357/150‑1 (MQ=11)
                                                                      tAAAACTGAGCGATAAGCCGCTTTCTTTTGTGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGCATCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAg                                                                             <  1:1390431/150‑1 (MQ=255)
                                                                         aaCTGAGCGATAAGCTGCTTTCATTTGTGTCTAGTGTCGGGGACAGTCATTCATATATCTGCCCCTCCAAAAGAATAAACCCGCCGAAGCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAgtgt                                                                          <  1:830470/150‑1 (MQ=11)
                                                                                     aGCCGCTGTCTTTTGGCTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCTTCCAACAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtca                                                              <  1:1478183/150‑1 (MQ=11)
                                                                                            ttctttTTGTTATAGTGTCGTGGAAAGTCATTCTTCTTTATGCCACTCCAAAAGTAAAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCt                                                       <  2:590074/150‑1 (MQ=11)
                                                                                            ttctttTGTTTTTTGTGTCGTGGAATGCGTTTTATATTTCTGGCACTCCAAAATTTTAAACCCGCCGTAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCt                                                       <  1:1114810/150‑1 (MQ=11)
                                                                                                  ttttgttaGTGTCGTGGACTGTGTTTGCTCTTTATGCCCCTCCACAATTTCAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGc                                                 <  2:559653/144‑1 (MQ=11)
                                                                                                  tgtttttaGTGTCGTGGAAAGTGTATGTTATTTCTGCCACTCAAAAAGTATAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGc                                                 <  2:552720/144‑1 (MQ=11)
                                                                                                   gggTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAcc                                                                                                       >  2:275312/1‑95 (MQ=18)
                                                                                                             tCGTGGAAAGGCTTTACTCTTACTGCCACTCCAAAAGTAAAAACCCGCCGCAGCGGGTTTTTACGTACAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAg                                      <  2:1565527/150‑1 (MQ=11)
                                                                                                                 ggACAGTGATTTTTCTTTATGGAACTCAAAAATTAGAAACCCGCCGCATCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaa                                  <  1:521666/150‑1 (MQ=11)
                                                                                                                     tccctatcatCTTTCTGCCCCTCCAAAAGTATAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAcc                              <  1:1593311/144‑1 (MQ=255)
                                                                                                                      gTCTTTCTTATTTCTGCCACTCAAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAccc                             <  1:1478727/150‑1 (MQ=11)
                                                                                                                                 ttCTGCCACTCCAAAAGAATAAACCCGCCGTAGCGGGTTTTTACGTAAATCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCggg                  <  2:1584449/150‑1 (MQ=11)
                                                                                                                                        ccTCCAAAAGTAAAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGtttttgc           <  1:1376022/150‑3 (MQ=11)
                                                                                                                                        ccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTg                                                                                                                >  2:855141/1‑49 (MQ=255)
                                                                                                                                                aGTACAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCa   <  1:1724454/150‑1 (MQ=255)
                                                                                                                                                 gTAAACACCAGCCGAAGCTGTTTTTTACGTAACACAGGTGACACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg  <  2:1661449/150‑1 (MQ=11)
                                                                                                                                                  |                                                                                                                                                    
CGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAG  >  NC_000913/3269810‑3270104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: