Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,288,032 C→A 9.3% intergenic (+22/‑58) yraH → / → yraI putative fimbrial‑like adhesin protein/putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,288,0320CA 9.3% 361.5 / 20.5 136intergenic (+22/‑58)yraH/yraIputative fimbrial‑like adhesin protein/putative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base C (62/61);  new base A (10/3);  total (72/64)
Fisher's exact test for biased strand distribution p-value = 8.41e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-01

Only 100 of 140 total aligned reads displayed.

TCCCGGCGGAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAAC  >  NC_000913/3287883‑3288181
                                                                                                                                                      |                                                                                                                                                     
tCCCGGCGGAATCCAATGGTATTGTCAATGTTGACCTCAGCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGAcc                                                                                                                                                       <  1:100982/150‑1 (MQ=255)
 cccGGCGGAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGAcc                                                                                                                                                       <  2:1665447/150‑1 (MQ=255)
 cccGGCGGAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCt                                                                                                                                                      >  2:906753/1‑150 (MQ=255)
      cggAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCTTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAg                                                                                                                                                 <  1:1307333/150‑1 (MQ=255)
          aTCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATaa                                                                                                                                             <  1:1547116/150‑1 (MQ=255)
           tCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAAc                                                                                                                                            <  1:990052/150‑1 (MQ=255)
            ccAATGGTATTGTCAATGTTGACCTCATCTCATACTACGTATCAACCGTAGTTCCATCAGAAATCAGCGCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGATAACg                                                                                                                                           <  1:694229/150‑1 (MQ=255)
            ccAATGGTATTGTCAATCTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCCTTATCTGACCTGTCAGATaaag                                                                                                                                           >  2:226381/1‑148 (MQ=255)
              aaTGGTATTGGCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGGTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGATAACGcc                                                                                                                                         <  2:833516/150‑1 (MQ=255)
                      tGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACAGGTCAGATAACGc                                                                                                                                          <  1:1378832/141‑1 (MQ=255)
                         cAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTttccttcc                                                                                                                              >  1:723002/1‑150 (MQ=255)
                           aTGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCctc                                                                                                                            <  2:1114845/150‑1 (MQ=255)
                                gaacTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGAAGACGCAAACGTTAACGTAACGCTGGATAACCGTTAAAACGTTAGGGCGTTATCAGACCAGTCAGATAACGCCCTTTTCCATACTCTAtca                                                                                                                       >  2:1128386/4‑149 (MQ=255)
                                gACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTtct                                                                                                                       <  1:1052501/150‑1 (MQ=255)
                                  ccTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGGCCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCg                                                                                                                     >  2:1014147/1‑150 (MQ=255)
                                  ccTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCg                                                                                                                     <  1:575842/150‑1 (MQ=255)
                                   cTCATCGCTTACAACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGt                                                                                                                    <  1:592008/150‑1 (MQ=255)
                                     cATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTg                                                                                                                  <  1:1206539/150‑1 (MQ=255)
                                     cATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTg                                                                                                                  <  1:161116/150‑1 (MQ=255)
                                     cATCGCTACTACGTATAAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTTTCAGATAACGCCCTTTTCCTTCCTCTTTCTAGTTGt                                                                                                                 >  2:471083/1‑150 (MQ=255)
                                         gCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTATTCCTTACTCTTTATCGTTGAATc                                                                                                              >  1:1135230/1‑150 (MQ=255)
                                              ctacGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTACCGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCACTTTTCCTTCATCTTTCTCGTTGTATCAGGtt                                                                                                         >  1:41117/1‑150 (MQ=255)
                                               tacGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTg                                                                                                        >  1:1262725/1‑150 (MQ=255)
                                                acGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGa                                                                                                       >  1:1281309/1‑150 (MQ=255)
                                                      cAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATa                                                                                                 <  2:543153/150‑1 (MQ=255)
                                                      cAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATa                                                                                                 <  1:126777/150‑1 (MQ=255)
                                                       aaCAGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACTCCCTTTTCCTTCCTCTTTCTCGTTGTATCATGTTGAAAAATaa                                                                                                >  2:408236/1‑150 (MQ=255)
                                                        aCCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAAc                                                                                               >  2:314866/1‑150 (MQ=255)
                                                         ccGTAGTTACATAAGAAAGCACACCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACt                                                                                              <  1:295560/150‑1 (MQ=255)
                                                          cGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATTACGCCCTTTTCCTTACTCTTTCTCGTTGTATCAGGTTGAAAAATTACTa                                                                                             >  2:1545606/1‑150 (MQ=255)
                                                          cGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTa                                                                                             >  1:559952/1‑150 (MQ=255)
                                                              gTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATTACGCCCTTTTACTTCCAATTTCTCGTTGTATAAGGTTGAAAAATAACTAttta                                                                                         >  2:1516258/1‑147 (MQ=255)
                                                               ttCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAATAATAACTATGTCa                                                                                        <  1:355094/150‑1 (MQ=255)
                                                                  cATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCaaaa                                                                                     >  2:30005/1‑150 (MQ=255)
                                                                  cATCAGAAATCACACCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCaaaa                                                                                     >  1:956590/1‑150 (MQ=255)
                                                                   aTCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTACTCTTTCACGTTGTATCAGTTTGAAAAATAACTATGTCaaaaa                                                                                    >  1:208543/1‑149 (MQ=255)
                                                                     cAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTAGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGa                                                                                  >  2:1071865/1‑150 (MQ=255)
                                                                      aGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTTTATCAGGTTGAAAAAAAACTATATAAAAAAGaa                                                                                 >  2:385934/1‑150 (MQ=255)
                                                                       gAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAAc                                                                                <  2:632410/150‑1 (MQ=255)
                                                                         aaTCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACAt                                                                              >  2:837892/1‑150 (MQ=255)
                                                                         aaTCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATGACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCACGTTGTATCAGGTTGAAAAATAACTATGTCCAAACGATCAt                                                                              >  2:232048/1‑150 (MQ=255)
                                                                              ccccAGGGGAAGCAGACGCTATCGTTAACTTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTACTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTcgcg                                                                         <  1:496207/150‑1 (MQ=255)
                                                                              ccccAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGACAGATAACGCCCTTTTCCTTCCACTTTCACGTTGTATAAGGGTGAAAAATAACTATTTCAAAACTAACATTcgcc                                                                         >  2:1388335/1‑149 (MQ=255)
                                                                                    ggggAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGat                                                                    <  1:51719/150‑1 (MQ=255)
                                                                                     gggAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCTTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGata                                                                   <  1:141581/150‑1 (MQ=255)
                                                                                     gggAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGata                                                                   >  2:1610168/1‑150 (MQ=255)
                                                                                           cAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTAAGCTAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAAAATTCGCGGTGATATTaagc                                                             >  1:80579/1‑148 (MQ=255)
                                                                                                 cTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAAGGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCttgttg                                                       <  2:1281309/150‑1 (MQ=255)
                                                                                                    tctttAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCATATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTgtgt                                                    <  1:385934/147‑1 (MQ=255)
                                                                                                     cGTTAACGTAACGCTGTATTAACGTTAATACGTTAGGGAGTTATCTGACCTGTAAGATAACGCCATTTTCCTTCCTCTTTCTCGTTGTTTCATGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTa                                                   <  1:226381/150‑1 (MQ=255)
                                                                                                     cGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAACAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTa                                                   <  1:435589/150‑1 (MQ=255)
                                                                                                          acgtaacgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTc                                              >  1:498547/1‑150 (MQ=255)
                                                                                                            gtaacgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGCCCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTg                                            >  1:157860/1‑150 (MQ=255)
                                                                                                             taacgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGt                                           <  2:1341419/150‑1 (MQ=255)
                                                                                                               acgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTAt                                         >  1:485718/1‑150 (MQ=255)
                                                                                                                cgCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTAtt                                        >  1:465997/1‑150 (MQ=255)
                                                                                                                cgCTGGATTACCGTTAATACGTTAGGGCGTTATATGACCTGTCAGATAACGCCCTTTTCATTACGCTTTCTCGTTGTTTAAGGTTGAAAAATACCTATGTCAAAACGAACATTCGCGGTGATATTTACCTTGTTGTGTAGCTtatttatt                                        >  2:1277646/1‑150 (MQ=255)
                                                                                                                 gCTGTATTACAGTTATTTCGTTAGGTCGTTTTCTGACCTGTAAGATAAAGCCCTTTTCCTTCCTCTTTCTAGTTGTTTCAGGTTGAAAAATATCTATGTCAAAAGTAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTg                                       <  1:950966/150‑1 (MQ=255)
                                                                                                                  cTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTgg                                      <  1:837892/150‑1 (MQ=255)
                                                                                                                    ggATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATTACGCCCTTTTACTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAAAATTCGCGGTGATATTAACCTTGTTGTGTACCTTCTGTATTGGcc                                    >  2:1376048/1‑150 (MQ=255)
                                                                                                                    ggATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTtgtg                                                     <  2:339778/133‑1 (MQ=255)
                                                                                                                    ggATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGcc                                    >  1:1726620/1‑150 (MQ=255)
                                                                                                                    ggATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGcc                                    <  2:1461121/150‑1 (MQ=255)
                                                                                                                     gATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGTTATTAACCTTGTTGTGTAGATTCTGTATTGGCCa                                   >  1:593870/1‑150 (MQ=255)
                                                                                                                     gATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCa                                   >  2:816129/1‑150 (MQ=255)
                                                                                                                     gATTACCGGTAATACGTTAGGGCGTTATCTGACCTTTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTGCTGTATTGTCCa                                   <  2:856037/150‑1 (MQ=255)
                                                                                                                        tACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGgc                                >  1:897654/1‑150 (MQ=255)
                                                                                                                        tACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGgc                                <  1:196251/150‑1 (MQ=255)
                                                                                                                         aCCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGgcg                               >  2:1386813/1‑150 (MQ=255)
                                                                                                                          ccGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGgcgc                              >  1:1187966/1‑150 (MQ=255)
                                                                                                                            gTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCtt                            >  1:1411212/1‑150 (MQ=255)
                                                                                                                             ttAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGAGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTg                           <  2:1435446/150‑1 (MQ=255)
                                                                                                                              tAATACGTTAGGGCGTTAACTGACCTGTAAGATAACGCCCTTTTCCTTACTCTTTAGCGGTGTATCAGTTTGAAAAATAACTATGTCAAAACGATAATTCGCGGTGATAATAACCTTTTTTTTTTGATTCTGTAATGGCCATGCGCTTgg                          >  2:1267059/1‑149 (MQ=255)
                                                                                                                              aaaTACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATACGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGc                          <  1:1547434/149‑1 (MQ=255)
                                                                                                                                aaggaaaaGGGCGTTATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCag                        >  1:81933/8‑150 (MQ=255)
                                                                                                                                aTACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCag                        >  1:1085653/1‑150 (MQ=255)
                                                                                                                                   cGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTGCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCaggag                     >  1:1430646/1‑150 (MQ=255)
                                                                                                                                   cGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCaggag                     <  2:1406217/150‑1 (MQ=255)
                                                                                                                                    gTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCaggagg                    <  1:212865/150‑1 (MQ=255)
                                                                                                                                        gggCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATc                <  1:1495729/150‑1 (MQ=255)
                                                                                                                                          gCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCTGGAGGAATCGt              <  2:1641539/150‑1 (MQ=255)
                                                                                                                                          gCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGt              >  1:651525/1‑150 (MQ=255)
                                                                                                                                          gCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGt              <  2:1074366/150‑1 (MQ=255)
                                                                                                                                          gCGTTATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGt              >  1:1004082/1‑150 (MQ=255)
                                                                                                                                          gCGTTATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGt              >  1:1603420/1‑150 (MQ=255)
                                                                                                                                            gTTATCTGGCCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGttt            <  1:1014147/150‑1 (MQ=255)
                                                                                                                                            gTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGttt            <  2:1262725/150‑1 (MQ=255)
                                                                                                                                            gTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGttt            <  1:1453334/150‑1 (MQ=255)
                                                                                                                                            gTTATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCTGGAGGAATCGttt            >  1:302996/1‑150 (MQ=255)
                                                                                                                                            gTTATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGttt            >  2:917200/1‑150 (MQ=255)
                                                                                                                                             ttATCTGACAGGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGtttt           >  2:1276110/1‑150 (MQ=255)
                                                                                                                                              tATCTGACCTGTCAGATAACGACCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTa          >  2:75643/1‑150 (MQ=255)
                                                                                                                                               agcTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTAc         <  1:941561/148‑1 (MQ=255)
                                                                                                                                               aTCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTAc         <  2:685144/150‑1 (MQ=255)
                                                                                                                                                tCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACa        >  1:753906/1‑150 (MQ=255)
                                                                                                                                                tCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGGCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACa        <  2:706035/150‑1 (MQ=255)
                                                                                                                                                  tGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGc      >  1:74610/1‑150 (MQ=255)
                                                                                                                                                  tGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGc      >  2:1158592/1‑150 (MQ=255)
                                                                                                                                                      cTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAAc  >  2:1706774/1‑150 (MQ=255)
                                                                                                                                                      cTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAAc  <  1:906753/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TCCCGGCGGAATCCAATGGTATTGTCAATGTTGACCTCATCGCTTACTACGTATCAACCGTAGTTCCATCAGAAATCACCCCAGGGGAAGCAGACGCTATCGTTAACGTAACGCTGGATTACCGTTAATACGTTAGGGCGTTATCTGACCTGTCAGATAACGCCCTTTTCCTTCCTCTTTCTCGTTGTATCAGGTTGAAAAATAACTATGTCAAAACGAACATTCGCGGTGATATTAACCTTGTTGTGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAAC  >  NC_000913/3287883‑3288181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: