Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,305,916 A→T 6.6% intergenic (‑99/+55) mtr ← / ← deaD tryptophan transporter of high affinity/ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,305,9160AT 6.6% 341.4 / 9.2 118intergenic (‑99/+55)mtr/deaDtryptophan transporter of high affinity/ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (57/53);  new base T (3/5);  total (60/58)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

CACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCA  >  NC_000913/3305767‑3306064
                                                                                                                                                     |                                                                                                                                                    
cacGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTa                                                                                                                                                      >  1:778288/1‑150 (MQ=255)
cacGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTa                                                                                                                                                      >  2:223838/1‑150 (MQ=255)
      gccAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATc                                                                                                                                                >  1:184970/1‑150 (MQ=255)
         aaGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTCTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAATAGACAGAAAAAAAGCCCCGATGGTAAAAATCggg                                                                                                                                             <  1:288040/150‑1 (MQ=255)
          agcagcGACGGTGACGTTGGGGTGGTGGTTAGGGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGGACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCgggg                                                                                                                                            <  1:879238/150‑1 (MQ=255)
           gcagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATTTTTACCATCGGGGc                                                                                                                                           <  1:806832/150‑1 (MQ=255)
           gcagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGc                                                                                                                                           <  2:823158/150‑1 (MQ=255)
            cagcGACGGTGACGTTTGGGTGTTGGTTAGTGTTGCCATTAGGGCTTCTCTCCAGTGAAAAATTGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATTTTTACCATCGGGGCt                                                                                                                                          <  2:954547/150‑1 (MQ=255)
               cGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGta                                                                                                                                       <  1:1632661/150‑1 (MQ=255)
               cGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTCGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATTTTTACCATCGGGGCtttt                                                                                                                                       <  1:634989/150‑4 (MQ=255)
               cGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTAGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATTTTTACCATCGGGGCtttt                                                                                                                                       <  2:1355938/150‑4 (MQ=255)
                 aCGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATATTGCGACTGCGTTGCTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATTTTTACCATCGGGGCtttttt                                                                                                                                     <  2:91130/150‑6 (MQ=255)
                      gACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATAtatta                                                                                                                                <  2:267241/150‑1 (MQ=255)
                        cGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATattatt                                                                                                                              >  2:690531/1‑150 (MQ=255)
                         gTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTAttt                                                                                                                             >  2:491959/1‑150 (MQ=255)
                         gTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTAttt                                                                                                                             <  2:711676/150‑1 (MQ=255)
                              ggtggtggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAg                                                                                                                        >  2:948379/1‑150 (MQ=255)
                               gtggtggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGa                                                                                                                       >  2:1077277/1‑150 (MQ=255)
                                  gtggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATtg                                                                                                                    <  2:778288/150‑1 (MQ=255)
                                    ggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATtgtg                                                                                                                  >  2:257102/1‑150 (MQ=255)
                                        aGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCg                                                                                                              >  1:1374096/1‑150 (MQ=255)
                                          tgtTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAATAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCt                                                                                                            <  1:650504/150‑1 (MQ=255)
                                          tgtTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCt                                                                                                            >  1:1625713/1‑150 (MQ=255)
                                          tgtTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCt                                                                                                            >  1:637932/1‑150 (MQ=255)
                                          tgtTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCt                                                                                                            <  2:728297/150‑1 (MQ=255)
                                          tgtTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGAGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAGATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCt                                                                                                            >  1:276727/1‑150 (MQ=255)
                                           gtTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTg                                                                                                           >  2:1085887/1‑150 (MQ=255)
                                            ttGCCATGAGGGCTTCTCTCCATTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGt                                                                                                          <  2:403587/150‑1 (MQ=255)
                                               ccATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCa                                                                                                       <  1:1366634/150‑1 (MQ=255)
                                                cATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAg                                                                                                      >  2:1168780/1‑150 (MQ=255)
                                                cATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAg                                                                                                      >  1:1125712/1‑150 (MQ=255)
                                                cATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGc                                                                                                     <  1:1721933/150‑1 (MQ=255)
                                                cATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGCTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGc                                                                                                     <  1:272670/150‑1 (MQ=255)
                                                cATGAGGGCTTCACTCCAGTGAAAAATAGTGCGACTGCGTAGTTATGCATAGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTTAAAATCGGGGATGTATATATTATTTTACAGATTGTGTACGCTGATCAg                                                                                                      >  2:720235/1‑150 (MQ=255)
                                                 aTGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGc                                                                                                     <  1:65814/150‑1 (MQ=255)
                                                  tGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCg                                                                                                    >  1:387477/1‑150 (MQ=255)
                                                    aGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCgat                                                                                                  <  1:1160884/150‑1 (MQ=255)
                                                    aGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGGTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCgat                                                                                                  <  2:641146/150‑1 (MQ=255)
                                                     gggCTTCTCTCCAGTGAAAAATAGTGCTACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCgatg                                                                                                 <  2:1150524/150‑1 (MQ=255)
                                                       gCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCgatgat                                                                                               <  2:210062/150‑1 (MQ=255)
                                                           gtctcCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCTCTGTACCAGTACACGTGTAATAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACg                                                                                           <  2:1075316/149‑1 (MQ=255)
                                                            tctcCAGTGAAAAATCGTGCGACTGCGGTGTTCTGCATTGCACTGTACCAGTACACGAGTACAAAAGCCAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGc                                                                                          <  1:1570883/150‑1 (MQ=255)
                                                            tctcCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGAtt                                                                                              >  1:1045489/1‑146 (MQ=255)
                                                            tctcCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGAtt                                                                                              <  2:1045489/146‑1 (MQ=255)
                                                               ccAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATc                                                                                       <  2:1374096/150‑1 (MQ=255)
                                                                  gTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGATGTTCAGCGATGATTACGCATcacc                                                                                    >  1:1484404/1‑150 (MQ=255)
                                                                           aGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacg                                                                           >  2:1671268/1‑150 (MQ=255)
                                                                             tGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAGAAAAGACCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTAAGCATCACCACCGAAacgac                                                                         <  2:1697215/150‑1 (MQ=255)
                                                                             tGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgac                                                                         <  1:967524/150‑1 (MQ=255)
                                                                              gCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacg                                                                        <  2:805265/150‑1 (MQ=255)
                                                                                 aCTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacgacg                                                                     <  1:966873/150‑1 (MQ=255)
                                                                                    gCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCACCGATGGTAAAATTCGGGGCTGTATATATTATTTTCCAGATTGTGTACGCTGTTCAGCGAGGATTACGAATCAACCCCGAAACGACAACGAcc                                                                  >  2:1260818/1‑150 (MQ=255)
                                                                                     cGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCg                                                                 >  2:572604/1‑150 (MQ=255)
                                                                                      gttgttATTCATTGCAATGTACAAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATTTTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCgg                                                                <  1:934713/150‑1 (MQ=255)
                                                                                           tATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGaa                                                           >  1:1171650/1‑150 (MQ=255)
                                                                                           tATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGaa                                                           >  2:1522911/1‑150 (MQ=255)
                                                                                            aTGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAat                                                          >  1:1697748/1‑150 (MQ=255)
                                                                                              gCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCCCCGAAACGACGACGACCGGTAGAatca                                                        >  1:1320262/1‑150 (MQ=255)
                                                                                                tttGCACTGTACAAGTACACGAGTACAAAAGACATAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATTATTACGCATCACCACCGAAACGACGACGACCGGTAGAatcatc                                                      <  2:1427548/149‑1 (MQ=255)
                                                                                                atgtcccTGTCCAAGTACACGAGTACAAAAGACTGAAAACAATCCGAGATGGTAAAATTCTGGTCTGTATATACTATTAAACAGTTTGTGTTCGCTGTTCAGAGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAatcatc                                                      <  2:254539/144‑1 (MQ=255)
                                                                                                aTTTCACTGTACCAGTACACTATTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGAATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGACACGACGACGACCGGTAGAatcatc                                                      <  2:681557/150‑1 (MQ=255)
                                                                                                  tGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCac                                                    >  2:1039120/1‑150 (MQ=255)
                                                                                                     aCTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTCTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCacgac                                                 <  1:965928/150‑1 (MQ=255)
                                                                                                     aCTGTACAAGTACACGAGAACAAAAGACAGAAAAAACCCCCCGATGGTAAAAATCGGGGCTGTATATATTATGTTACAGATTTTGTTTGCTGTTCAGCGATGATTACGCTTCACCACCGAAACGACGACGACCGGTAGAATCATCacgac                                                 <  2:269642/150‑1 (MQ=255)
                                                                                                      cTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCacgacg                                                >  1:1066905/1‑150 (MQ=255)
                                                                                                           ccAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTCCGCATCACCACCGAAACGACGACTACCGGTAGAATCATCACGACGCGGAg                                           >  1:312394/1‑150 (MQ=255)
                                                                                                            cAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAgc                                          <  1:223838/150‑1 (MQ=255)
                                                                                                              gTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAgcgc                                        >  1:1418925/1‑150 (MQ=255)
                                                                                                              gTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAgcgc                                        >  1:1630354/1‑150 (MQ=255)
                                                                                                              gTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAgcgc                                        <  1:948379/150‑1 (MQ=255)
                                                                                                                acacGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCgg                                      <  1:1007799/150‑1 (MQ=255)
                                                                                                                acacGAGAACAAAAGACATAAAAAAAGCCCCGTTGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCgg                                      <  1:753824/150‑1 (MQ=255)
                                                                                                                 cacGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGc                                     >  1:528732/1‑150 (MQ=255)
                                                                                                                 cacGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGc                                     <  1:1077277/150‑1 (MQ=255)
                                                                                                                 cacGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGc                                     >  2:1666615/1‑150 (MQ=255)
                                                                                                                 cacGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGc                                     <  2:1185328/150‑1 (MQ=255)
                                                                                                                   cGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCt                                   >  2:1352546/1‑150 (MQ=255)
                                                                                                                       tACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCac                               <  1:657652/150‑1 (MQ=255)
                                                                                                                        aCAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCacg                              >  2:965258/1‑150 (MQ=255)
                                                                                                                          aaaaGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCacgac                            <  1:1594653/150‑1 (MQ=255)
                                                                                                                             aGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATAACGACGCGGAGCGCGGCCTTCACGACGtt                         >  2:791864/1‑150 (MQ=255)
                                                                                                                               aCAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTcg                       <  2:1171650/150‑1 (MQ=255)
                                                                                                                                  gAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCTTCACCACCGAAACGACGACGACCGGGAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTcgccg                    >  1:1436466/1‑150 (MQ=255)
                                                                                                                                  gAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGCCCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTcgccg                    >  2:519711/1‑150 (MQ=255)
                                                                                                                                  gAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTcgccg                    >  2:964853/1‑150 (MQ=255)
                                                                                                                                  gAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATAACCACCGAAACGACGACGACCGGTAGAATCATAACGACGAGGAGCGCGGCCTTCACGACGTTcgcgg                    >  1:45587/1‑148 (MQ=255)
                                                                                                                                  aaaaaaacGCCCCGATGGAAAAAATCGGGGCTGTATATATTATTTTACAGCTTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTcgccg                    <  2:1572294/149‑1 (MQ=255)
                                                                                                                                    aaaaaaGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCt                  >  1:1409866/1‑150 (MQ=255)
                                                                                                                                      aaaaGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTaa                >  2:1577432/1‑150 (MQ=255)
                                                                                                                                       acaGCCCCGATTTTTACCATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTaaa               >  1:430332/3‑150 (MQ=255)
                                                                                                                                         aGCCCCGATTTTTACCATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAac             >  1:1629699/1‑150 (MQ=255)
                                                                                                                                         aGCCCCGATGGTAAAAATCGTGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAac             <  1:851612/150‑1 (MQ=255)
                                                                                                                                          gCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAacg            >  2:210528/1‑150 (MQ=255)
                                                                                                                                           ccccGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGATCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAccgc           >  1:1508848/1‑149 (MQ=255)
                                                                                                                                           ccccGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAacga           >  2:1208433/1‑150 (MQ=255)
                                                                                                                                            cccGATCGTAAAAAACGGGGCTGTATATATTATTATACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACTACGACTACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAacgac          <  1:1106157/150‑1 (MQ=255)
                                                                                                                                             ccGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAacgacg         >  2:1548991/1‑150 (MQ=255)
                                                                                                                                                  ggTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCAt    >  2:790595/1‑150 (MQ=255)
                                                                                                                                                  ggTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGAGGCCTTCACGACGTTCTCAGCTAAAACTACGAACaa    >  1:1213606/1‑149 (MQ=255)
                                                                                                                                                    tAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATca  >  2:1325508/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CACGCCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACCATCA  >  NC_000913/3305767‑3306064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: