Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,322,040 A→G 7.3% intergenic (‑427/+32) yhbX ← / ← leuU putative EptAB family phosphoethanolamine transferase, inner membrane protein/tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,322,0400AG 7.3% 363.1 / 13.4 127intergenic (‑427/+32)yhbX/leuUputative EptAB family phosphoethanolamine transferase, inner membrane protein/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base A (53/62);  new base G (7/2);  total (60/67)
Fisher's exact test for biased strand distribution p-value = 8.81e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 131 total aligned reads displayed.

AAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGC  >  NC_000913/3321894‑3322189
                                                                                                                                                  |                                                                                                                                                     
aaaGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGa                                                                                                                                                    <  2:615691/150‑1 (MQ=255)
aaaGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGa                                                                                                                                                    >  2:70778/1‑150 (MQ=255)
aaaGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGa                                                                                                                                                    >  1:1575351/1‑150 (MQ=255)
   gTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGc                                                                                                                                                 >  2:1232513/1‑150 (MQ=255)
    tGCCAATTATGTCAGTAGAAGGTAAAAATTTATAAATAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCt                                                                                                                                                <  1:792564/150‑1 (MQ=255)
        aaTTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTc                                                                                                                                            >  1:1332335/1‑150 (MQ=255)
        aaTTATGTCAGTAGAAGGGAAAAATTTATAACCAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTc                                                                                                                                            <  2:54878/150‑1 (MQ=255)
             tGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCTTTTCAAGTTACCAGCTACGAGTAAAGCAACTTGACGAGATACATATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGt                                                                                                                                       <  1:528477/150‑1 (MQ=255)
             tGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGt                                                                                                                                       <  1:240375/150‑1 (MQ=255)
                  ttAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCTTCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCTGACCATGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCtt                                                                                                                                  <  1:625917/149‑1 (MQ=255)
                   tAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACGAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCttt                                                                                                                                 <  1:1206240/150‑1 (MQ=255)
                   tAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTGAAAAGCGTCTtttt                                                                                                                                 <  1:1401548/150‑1 (MQ=255)
                     taaGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTc                                                                                                                               <  1:835281/149‑1 (MQ=255)
                     gAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAATCAACTGGACGAGATACAGATATCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCCTCGTCTCTTTTc                                                                                                                               >  1:1139514/1‑150 (MQ=255)
                      aaGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCt                                                                                                                              <  1:466669/150‑1 (MQ=255)
                       aGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACTATATACAGCTACATGACACCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGGCTCTTTTCTg                                                                                                                             >  2:1468619/1‑150 (MQ=255)
                         ggAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAGAGACGCTGAAAAGCGTCTTTTTTcgttt                                                                                                                           <  1:237968/150‑5 (MQ=255)
                         ggAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGa                                                                                                                           <  2:1421783/150‑1 (MQ=255)
                             aaaTTTATAACTAATGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAAttt                                                                                                                       <  2:520316/150‑1 (MQ=255)
                             aaaTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGATATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAAttt                                                                                                                       <  2:893330/150‑1 (MQ=255)
                               aTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTgg                                                                                                                     <  1:622689/150‑1 (MQ=255)
                                 ttATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTa                                                                                                                   <  1:1232513/150‑1 (MQ=255)
                                  aataACTAAGGCGTACCGGCACCATCGATTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTAc                                                                                                                  <  1:165735/149‑1 (MQ=255)
                                     aaCTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGa                                                                                                               >  1:995781/1‑150 (MQ=255)
                                      aCTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGATTTTGGTACCGAg                                                                                                              >  1:615476/1‑150 (MQ=255)
                                           ggCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAgtccgg                                                                                                         >  1:838532/1‑145 (MQ=255)
                                             cGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCg                                                                                                                                        <  2:876710/117‑1 (MQ=255)
                                              gTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGATAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGAc                                                                                                      <  1:1030348/150‑1 (MQ=255)
                                              gTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGAc                                                                                                      <  2:1398897/150‑1 (MQ=255)
                                                  cGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTTGTACCGAGGACGGGACTTGa                                                                                                  <  1:239108/150‑1 (MQ=255)
                                                    gCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAACTTGGTACCGAGGACGGGACTTGAAc                                                                                                <  1:997780/150‑1 (MQ=255)
                                                       ccATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCg                                                                                             <  2:1525843/150‑1 (MQ=255)
                                                         aTCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCATa                                                                                           <  2:765437/150‑1 (MQ=255)
                                                            gTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCATTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGCACGGGACTTGAACCCGTAAGc                                                                                        >  1:1200300/1‑150 (MQ=255)
                                                             tttCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGcc                                                                                       <  2:234171/150‑1 (MQ=255)
                                                              ttCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGccc                                                                                      <  2:187820/150‑1 (MQ=255)
                                                              ttCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGccc                                                                                      <  1:1263513/150‑1 (MQ=255)
                                                                cAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTCAGCCCTa                                                                                    >  1:1430826/1‑150 (MQ=255)
                                                                cAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCtt                                                                                    >  2:597523/1‑149 (MQ=255)
                                                                cAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTACAGCGTCTCATTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGcccaa                                                                                    >  1:1422703/1‑148 (MQ=255)
                                                                   ggTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCTCGACCAAAACGAAAATAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTg                                                                                 <  2:128151/150‑1 (MQ=255)
                                                                   ggTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTg                                                                                 <  2:133424/150‑1 (MQ=255)
                                                                      aCCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGc                                                                              <  1:402202/150‑1 (MQ=255)
                                                                      aCCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGc                                                                              <  2:821376/150‑1 (MQ=255)
                                                                        cAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCAc                                                                            <  1:98105/150‑1 (MQ=255)
                                                                         aGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACt                                                                           <  1:717300/150‑1 (MQ=255)
                                                                          gCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTa                                                                          >  2:405165/1‑150 (MQ=255)
                                                                          gCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTa                                                                          >  1:1666924/1‑150 (MQ=255)
                                                                          gCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTATCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTa                                                                          >  2:693153/1‑150 (MQ=255)
                                                                            tACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTacc                                                                        <  2:1625099/150‑1 (MQ=255)
                                                                              cGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccac                                                                      >  1:1714383/1‑150 (MQ=255)
                                                                              cGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccac                                                                      <  2:552303/150‑1 (MQ=255)
                                                                               taGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccacc                                                                     <  1:457212/149‑1 (MQ=255)
                                                                               gAGTAAAGCAACTGGACGAGATACAGCTACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAACACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccacc                                                                     <  1:1271733/150‑1 (MQ=255)
                                                                               gAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccacc                                                                     <  1:331051/150‑1 (MQ=255)
                                                                                aGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCt                                                                    <  2:12643/150‑1 (MQ=255)
                                                                                   aaaGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCaa                                                                 >  2:482917/1‑150 (MQ=255)
                                                                                   aaaGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCaa                                                                 >  2:731720/1‑150 (MQ=255)
                                                                                    aaGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGCGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAg                                                                >  2:179418/1‑150 (MQ=255)
                                                                                        aaCTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTATCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAg                                                            >  1:1371275/1‑150 (MQ=255)
                                                                                         aCTCGACGAGATACAGATACCTGACCAGCATTCCTCAGACCAGGACCAAAACGAAAATCGACTCTTTTCAGAGTCTCTTTTCTGGAATGTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGc                                                           <  1:359447/150‑1 (MQ=255)
                                                                                          cTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCg                                                          <  1:394031/150‑1 (MQ=255)
                                                                                            ggACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCgtg                                                        >  1:1487836/1‑150 (MQ=255)
                                                                                             gACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTTAACCCGTAAGCCCTATAGGGCACTACCACCTCAAGGTAGCgtgt                                                       >  2:1056828/1‑150 (MQ=255)
                                                                                               cGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCt                                                     <  2:1092386/150‑1 (MQ=255)
                                                                                                 agatacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACGCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAc                                                   >  2:1063653/1‑150 (MQ=255)
                                                                                                 agatacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAc                                                   <  2:508835/150‑1 (MQ=255)
                                                                                                  gatacagatacCTGACAACCATTCCTCAGACCAGGACCATAACGAAAAAAGACGCGTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTTGGCACTACCACCTCAAGGTAGCGTGTCTAcc                                                  <  2:704284/150‑1 (MQ=255)
                                                                                                  gatacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAcc                                                  >  1:407675/1‑150 (MQ=255)
                                                                                                  gatacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACACGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAcc                                                  >  1:301176/1‑150 (MQ=255)
                                                                                                  gatacagatacCTGACAACCATTCCTCAGACCAGGAACAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTATGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAcc                                                  <  2:760523/150‑1 (MQ=255)
                                                                                                   atacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCa                                                 <  1:509870/150‑1 (MQ=255)
                                                                                                     acagatacCTGACAACCATTCCTCAGACCAGGACCAAACCGAAAAAAGACGCTATTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGTACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAt                                               <  1:1093190/150‑1 (MQ=255)
                                                                                                       agatacCTGACAACCATTCATCAGACCAGGACCAAAACGAAAAAAGACGCCTTTCAGCGTCTCTTTTAAGGAATTTGGTACCGAGGACGGGACTACAACCCGTAAGACCTAGTGGGCACTAACACCTCAAGGTAGCGTGTCTAGCAAATc                                             >  1:1082967/1‑150 (MQ=255)
                                                                                                        gatacCTGACAACCTTTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTcc                                            <  1:188472/150‑1 (MQ=255)
                                                                                                            ccTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGGGTCTACCAATAccacca                                        >  2:1228565/1‑150 (MQ=255)
                                                                                                              tGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCcccacc                                      >  2:564798/1‑150 (MQ=255)
                                                                                                              tGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCaccacc                                      >  2:1193641/1‑150 (MQ=255)
                                                                                                               taCAACCATTACTCCGAACAGGACCAAAAATAAAAAAGCAGCTTTTCAGAGTCTCTTTTCTTGAATTTGGTACCGAGGACGGTACTTGAACCCGGAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCt                                     <  2:954599/149‑1 (MQ=255)
                                                                                                               gACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCaccaccg                                     >  2:583817/1‑149 (MQ=255)
                                                                                                                 cAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCCCCTCg                                   >  1:715390/1‑150 (MQ=255)
                                                                                                                  aaCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCgg                                  <  1:1733700/150‑1 (MQ=255)
                                                                                                                   aCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGc                                 <  1:1354772/150‑1 (MQ=255)
                                                                                                                   aCCAATCCTCAGACCAGGACCAAAACGAATACAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGCTCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGc                                 <  2:119862/150‑1 (MQ=255)
                                                                                                                         ccTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGAt                           >  1:1049348/1‑150 (MQ=255)
                                                                                                                                ccAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACtt                    >  1:1113998/1‑150 (MQ=255)
                                                                                                                                ccAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACtt                    >  1:339180/1‑150 (MQ=255)
                                                                                                                                   ggACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACttttt                 >  2:1404525/1‑150 (MQ=255)
                                                                                                                                   ggACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACttttt                 >  1:536442/1‑150 (MQ=255)
                                                                                                                                    caCCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTa                <  2:360099/149‑1 (MQ=255)
                                                                                                                                     aCCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAg               >  1:597902/1‑150 (MQ=255)
                                                                                                                                       cAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGtt             >  1:768201/1‑150 (MQ=255)
                                                                                                                                        ttccagAAAAGAGACGCTGAAAAGCGTCTTTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCAACTCGGCACGGATACTACTGTTTAGttg            >  1:1056599/6‑149 (MQ=255)
                                                                                                                                           cagAAAAGAGACGCTGAAAAGCGTCTTTTTTCTGTAATTTGGTACCGAGGACGGTACTTGAACCCGTAAGCCCTATTGGGCACTACCACATCAAGGTAGCTATTCTACCAATACCACCACCTCGCCACGGATACAACTGTTTAGTTCtgg         >  2:277436/3‑150 (MQ=255)
                                                                                                                                           cagAAAAGAGACGCTGAAAAGCGTCTTTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCggg         >  2:1679544/3‑150 (MQ=255)
                                                                                                                                            agAAAAGAGACGCTGAAAAGCGTCTTTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGa        >  2:1186350/2‑150 (MQ=255)
                                                                                                                                             gAAAAGAGACGCTGAAAAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCGCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAACAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGat       >  1:1046640/1‑150 (MQ=255)
                                                                                                                                               aaaaaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGatat     <  2:114514/150‑1 (MQ=255)
                                                                                                                                                aaaaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATc    >  2:1232665/1‑150 (MQ=255)
                                                                                                                                                  aaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGTCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGc  <  1:245247/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
AAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGC  >  NC_000913/3321894‑3322189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: