Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,322,048 T→G 7.7% intergenic (‑435/+24) yhbX ← / ← leuU putative EptAB family phosphoethanolamine transferase, inner membrane protein/tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,322,0480TG 7.7% 352.7 / 18.0 131intergenic (‑435/+24)yhbX/leuUputative EptAB family phosphoethanolamine transferase, inner membrane protein/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base T (57/63);  new base G (7/3);  total (64/67)
Fisher's exact test for biased strand distribution p-value = 2.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.15e-01

Only 100 of 134 total aligned reads displayed.

GCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGC  >  NC_000913/3321899‑3322197
                                                                                                                                                     |                                                                                                                                                     
gCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTTAAGCAAATGGACGAGAAACAGATACCTGACAACCATTCCTCAGACCCGGACCAAAACGAATAAAGTCGCtt                                                                                                                                                       >  1:191440/1‑150 (MQ=255)
   aaTTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTc                                                                                                                                                    >  1:1332335/1‑150 (MQ=255)
   aaTTATGTCAGTAGAAGGGAAAAATTTATAACCAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTc                                                                                                                                                    <  2:54878/150‑1 (MQ=255)
        tGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCTTTTCAAGTTACCAGCTACGAGTAAAGCAACTTGACGAGATACATATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGt                                                                                                                                               <  1:528477/150‑1 (MQ=255)
             ttAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCTTCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCTGACCATGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCtt                                                                                                                                          <  1:625917/149‑1 (MQ=255)
              tAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTGAAAAGCGTCTtttt                                                                                                                                         <  1:1401548/150‑1 (MQ=255)
                gAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAATCAACTGGACGAGATACAGATATCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCCTCGTCTCTTTTc                                                                                                                                       >  1:1139514/1‑150 (MQ=255)
                 aaGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCt                                                                                                                                      <  1:466669/150‑1 (MQ=255)
                  aGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACTATATACAGCTACATGACACCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGGCTCTTTTCTg                                                                                                                                     >  2:1468619/1‑150 (MQ=255)
                    ggAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAGAGACGCTGAAAAGCGTCTTTTTTcgttt                                                                                                                                   <  1:237968/150‑5 (MQ=255)
                    ggAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGa                                                                                                                                   <  2:1421783/150‑1 (MQ=255)
                    ggAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGa                                                                                                                                   >  2:525675/1‑150 (MQ=255)
                     gAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAGAGACGCTGAAAAGCGTCTTTTTTcgtttt                                                                                                                                  <  2:1684118/150‑6 (MQ=255)
                       aaaaTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACATGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCGGGGGCTCTTTTCTGGAttt                                                                                                                                >  1:1219487/1‑150 (MQ=255)
                        aaaTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGATATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAAttt                                                                                                                               <  2:893330/150‑1 (MQ=255)
                        aaaTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAAttt                                                                                                                               <  2:589304/150‑1 (MQ=255)
                          aTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTgg                                                                                                                             <  1:622689/150‑1 (MQ=255)
                            ttATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTa                                                                                                                           <  1:1232513/150‑1 (MQ=255)
                             aataACTAAGGCGTACCGGCACCATCGATTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTAc                                                                                                                          <  1:165735/149‑1 (MQ=255)
                                 aCTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGATTTTGGTACCGAg                                                                                                                      >  1:615476/1‑150 (MQ=255)
                                      ggCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAgtccgg                                                                                                                 >  1:838532/1‑145 (MQ=255)
                                        cGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCg                                                                                                                                                >  1:876710/1‑117 (MQ=255)
                                        cGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGa                                                                                                               <  1:1422044/150‑1 (MQ=255)
                                         gTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGATAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGAc                                                                                                              <  1:1030348/150‑1 (MQ=255)
                                         gTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGAc                                                                                                              <  2:1398897/150‑1 (MQ=255)
                                             cGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTTGTACCGAGGACGGGACTTGa                                                                                                          <  1:239108/150‑1 (MQ=255)
                                               gCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAACTTGGTACCGAGGACGGGACTTGAAc                                                                                                        <  1:997780/150‑1 (MQ=255)
                                                  ccATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCg                                                                                                     <  2:1525843/150‑1 (MQ=255)
                                                       gTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCATTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGCACGGGACTTGAACCCGTAAGc                                                                                                >  1:1200300/1‑150 (MQ=255)
                                                         ttCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGccc                                                                                              <  2:187820/150‑1 (MQ=255)
                                                         ttCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGccc                                                                                              <  1:1263513/150‑1 (MQ=255)
                                                           cAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCtt                                                                                            >  2:597523/1‑149 (MQ=255)
                                                           cAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTACAGCGTCTCATTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGcccaa                                                                                            >  1:1422703/1‑148 (MQ=255)
                                                              ggTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCTCGACCAAAACGAAAATAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTg                                                                                         <  2:128151/150‑1 (MQ=255)
                                                              ggTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTg                                                                                         <  2:133424/150‑1 (MQ=255)
                                                               gTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTgg                                                                                        >  2:698124/1‑150 (MQ=255)
                                                                 aCCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGc                                                                                      <  2:821376/150‑1 (MQ=255)
                                                                 aCCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGc                                                                                      <  1:402202/150‑1 (MQ=255)
                                                                 aCCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGc                                                                                      <  1:1670501/150‑1 (MQ=255)
                                                                   cAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCAc                                                                                    <  1:98105/150‑1 (MQ=255)
                                                                       tACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTacc                                                                                <  2:240496/150‑1 (MQ=255)
                                                                       tACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTacc                                                                                <  2:1625099/150‑1 (MQ=255)
                                                                        aCGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccc                                                                               >  2:848722/1‑149 (MQ=255)
                                                                         cGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccac                                                                              <  2:552303/150‑1 (MQ=255)
                                                                         cGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccac                                                                              >  1:1714383/1‑150 (MQ=255)
                                                                         cGAGTAAAGCAACTGGACGAGATACAGAAACCTGACAACCATTCCTCAGCCCAGGACCAAAACGAAAAAAGACGCTTTTCGGCGTCTCTTTTCAGGAATATGGTACCGAGCACGGAACTTGCACCCCTCCGCCCTCATGGGCACTaccac                                                                              >  1:1125192/1‑150 (MQ=255)
                                                                          taGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccacc                                                                             <  1:457212/149‑1 (MQ=255)
                                                                          gAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccacc                                                                             <  1:331051/150‑1 (MQ=255)
                                                                           aGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGAACCGAGGACGGGACTTGCACCCGTAAGCCCTATTGGGCACTACCACCt                                                                            >  2:124407/1‑150 (MQ=255)
                                                                              aaaGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTGTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCaa                                                                         >  2:477449/1‑150 (MQ=255)
                                                                              aaaGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCaa                                                                         >  2:731720/1‑150 (MQ=255)
                                                                              aaaGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCaa                                                                         >  2:482917/1‑150 (MQ=255)
                                                                               aaGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGCGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAg                                                                        >  2:179418/1‑150 (MQ=255)
                                                                                  cAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTa                                                                     <  1:345967/150‑1 (MQ=255)
                                                                                     cTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCg                                                                  <  1:394031/150‑1 (MQ=255)
                                                                                       ggACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCgtg                                                                >  1:1487836/1‑150 (MQ=255)
                                                                                        gACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTTAACCCGTAAGCCCTATAGGGCACTACCACCTCAAGGTAGCgtgt                                                               >  2:1056828/1‑150 (MQ=255)
                                                                                          cGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCt                                                             <  2:1092386/150‑1 (MQ=255)
                                                                                            agatacagatacCTGACAACCATTCCTCAGACCAGTACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAc                                                           <  1:77917/150‑1 (MQ=255)
                                                                                            agatacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAc                                                           <  2:508835/150‑1 (MQ=255)
                                                                                             gatacagatacCTGACAACCATTCCTCAGACCAGGACCATAACGAAAAAAGACGCGTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTTGGCACTACCACCTCAAGGTAGCGTGTCTAcc                                                          <  2:704284/150‑1 (MQ=255)
                                                                                             gatacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACACGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAcc                                                          >  1:301176/1‑150 (MQ=255)
                                                                                             gatacagatacCTGACAACCATTCCTCAGACCAGGAACAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTATGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAcc                                                          <  2:760523/150‑1 (MQ=255)
                                                                                             gatacagatacCTGACAACCAATCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAATCCGTAAGCCCTATTGGGCACTAACACCTCAAGGTAGCGTGTCTAcc                                                          <  2:1394311/150‑1 (MQ=255)
                                                                                              atacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCa                                                         <  1:509870/150‑1 (MQ=255)
                                                                                                acagatacCTGACAACCATTCCTCAGACCAGGACCAAACCGAAAAAAGACGCTATTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGTACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAt                                                       <  1:1093190/150‑1 (MQ=255)
                                                                                                acagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAt                                                       <  1:1523202/150‑1 (MQ=255)
                                                                                                 cagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAtt                                                      >  1:251756/1‑150 (MQ=255)
                                                                                                  agatacCTGACAACCATTCATCAGACCAGGACCAAAACGAAAAAAGACGCCTTTCAGCGTCTCTTTTAAGGAATTTGGTACCGAGGACGGGACTACAACCCGTAAGACCTAGTGGGCACTAACACCTCAAGGTAGCGTGTCTAGCAAATc                                                     >  1:1082967/1‑150 (MQ=255)
                                                                                                     tacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCTAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccac                                                  >  1:1305484/1‑150 (MQ=255)
                                                                                                     tacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccac                                                  <  1:698124/150‑1 (MQ=255)
                                                                                                       ccTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGGGTCTACCAATAccacca                                                >  2:1228565/1‑150 (MQ=255)
                                                                                                         tGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCaccacc                                              >  2:1193641/1‑150 (MQ=255)
                                                                                                          taCAACCATTACTCCGAACAGGACCAAAAATAAAAAAGCAGCTTTTCAGAGTCTCTTTTCTTGAATTTGGTACCGAGGACGGTACTTGAACCCGGAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCt                                             <  2:954599/149‑1 (MQ=255)
                                                                                                          gACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCaccaccg                                             >  2:583817/1‑149 (MQ=255)
                                                                                                            cAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCCCCTCg                                           >  1:715390/1‑150 (MQ=255)
                                                                                                             aaCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCgg                                          <  1:1733700/150‑1 (MQ=255)
                                                                                                              aCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCCCCTCGGc                                         >  1:402645/1‑150 (MQ=255)
                                                                                                              aCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGc                                         <  1:1354772/150‑1 (MQ=255)
                                                                                                              aCCAATCCTCAGACCAGGACCAAAACGAATACAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGCTCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGc                                         <  2:119862/150‑1 (MQ=255)
                                                                                                                    ccTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGAt                                   >  1:1049348/1‑150 (MQ=255)
                                                                                                                      tCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGAtac                                 <  2:757167/150‑1 (MQ=255)
                                                                                                                          aCCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCCCCACCTCGGCACGGATactact                             >  2:1014056/1‑150 (MQ=255)
                                                                                                                           ccAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACtt                            >  1:339180/1‑150 (MQ=255)
                                                                                                                              ggACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACttttt                         >  2:1404525/1‑150 (MQ=255)
                                                                                                                              ggACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACttttt                         >  1:536442/1‑150 (MQ=255)
                                                                                                                               caCCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTa                        <  2:360099/149‑1 (MQ=255)
                                                                                                                                aCCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAg                       >  1:597902/1‑150 (MQ=255)
                                                                                                                                  cAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGtt                     >  1:768201/1‑150 (MQ=255)
                                                                                                                                      cagAAAAGAGACGCTGAAAAGCGTCTTTTTTCTGTAATTTGGTACCGAGGACGGTACTTGAACCCGTAAGCCCTATTGGGCACTACCACATCAAGGTAGCTATTCTACCAATACCACCACCTCGCCACGGATACAACTGTTTAGTTCtgg                 >  2:277436/3‑150 (MQ=255)
                                                                                                                                       agAAAAGAGACGCTGAAAAGCGTCTTTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGa                >  2:1186350/2‑150 (MQ=255)
                                                                                                                                        gAAAAGAGACGCTGAAAAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCGCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAACAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGat               >  1:1046640/1‑150 (MQ=255)
                                                                                                                                          aaaaaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGatat             <  2:114514/150‑1 (MQ=255)
                                                                                                                                           aaaaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATc            >  2:1232665/1‑150 (MQ=255)
                                                                                                                                             aaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGTCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGc          <  1:245247/150‑1 (MQ=255)
                                                                                                                                             aaGACGCTTTTCAGCGTCTCGTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTTTCTACCAATTCCACCACCTCGGCACGGATACAACTTTTTAGTTCGGGATATCGc          >  1:1062882/1‑150 (MQ=255)
                                                                                                                                                aCGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTgg       >  2:713960/1‑150 (MQ=255)
                                                                                                                                                 cGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGt      >  2:839497/1‑150 (MQ=255)
                                                                                                                                                     tttCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGc  >  1:454595/1‑150 (MQ=255)
                                                                                                                                                     tttCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGc  <  2:1159264/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGC  >  NC_000913/3321899‑3322197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: