Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,322,049 T→A 7.5% intergenic (‑436/+23) yhbX ← / ← leuU putative EptAB family phosphoethanolamine transferase, inner membrane protein/tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,322,0490TA 7.5% 370.4 / 14.3 131intergenic (‑436/+23)yhbX/leuUputative EptAB family phosphoethanolamine transferase, inner membrane protein/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base T (57/64);  new base A (7/3);  total (64/67)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01

Only 100 of 134 total aligned reads displayed.

AATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGCT  >  NC_000913/3321902‑3322198
                                                                                                                                                   |                                                                                                                                                     
aaTTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTc                                                                                                                                                     >  1:1332335/1‑150 (MQ=255)
aaTTATGTCAGTAGAAGGGAAAAATTTATAACCAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTc                                                                                                                                                     <  2:54878/150‑1 (MQ=255)
     tGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCTTTTCAAGTTACCAGCTACGAGTAAAGCAACTTGACGAGATACATATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGt                                                                                                                                                <  1:528477/150‑1 (MQ=255)
          ttAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCTTCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCTGACCATGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCtt                                                                                                                                           <  1:625917/149‑1 (MQ=255)
           tAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTGAAAAGCGTCTtttt                                                                                                                                          <  1:1401548/150‑1 (MQ=255)
             gAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAATCAACTGGACGAGATACAGATATCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCCTCGTCTCTTTTc                                                                                                                                        >  1:1139514/1‑150 (MQ=255)
              aaGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCt                                                                                                                                       <  1:466669/150‑1 (MQ=255)
               aGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACTATATACAGCTACATGACACCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGGCTCTTTTCTg                                                                                                                                      >  2:1468619/1‑150 (MQ=255)
                 ggAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAGAGACGCTGAAAAGCGTCTTTTTTcgttt                                                                                                                                    <  1:237968/150‑5 (MQ=255)
                 ggAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGa                                                                                                                                    >  2:525675/1‑150 (MQ=255)
                 ggAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGa                                                                                                                                    <  2:1421783/150‑1 (MQ=255)
                  gAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAGAGACGCTGAAAAGCGTCTTTTTTcgtttt                                                                                                                                   <  2:1684118/150‑6 (MQ=255)
                    aaaaTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACATGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCGGGGGCTCTTTTCTGGAttt                                                                                                                                 >  1:1219487/1‑150 (MQ=255)
                     aaaTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGATATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAAttt                                                                                                                                <  2:893330/150‑1 (MQ=255)
                     aaaTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAAttt                                                                                                                                <  2:589304/150‑1 (MQ=255)
                       aTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTgg                                                                                                                              <  1:622689/150‑1 (MQ=255)
                         ttATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTa                                                                                                                            <  1:1232513/150‑1 (MQ=255)
                              aCTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGATTTTGGTACCGAg                                                                                                                       >  1:615476/1‑150 (MQ=255)
                                   ggCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAgtccgg                                                                                                                  >  1:838532/1‑145 (MQ=255)
                                     cGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCg                                                                                                                                                 >  1:876710/1‑117 (MQ=255)
                                     cGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGa                                                                                                                <  1:1422044/150‑1 (MQ=255)
                                      gTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGATAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGAc                                                                                                               <  1:1030348/150‑1 (MQ=255)
                                      gTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGAc                                                                                                               <  2:1398897/150‑1 (MQ=255)
                                          cGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTTGTACCGAGGACGGGACTTGa                                                                                                           <  1:239108/150‑1 (MQ=255)
                                            gCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAACTTGGTACCGAGGACGGGACTTGAAc                                                                                                         <  1:997780/150‑1 (MQ=255)
                                               ccATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCg                                                                                                      <  2:1525843/150‑1 (MQ=255)
                                                    gTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCATTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGCACGGGACTTGAACCCGTAAGc                                                                                                 >  1:1200300/1‑150 (MQ=255)
                                                      ttCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGccc                                                                                               <  2:187820/150‑1 (MQ=255)
                                                      ttCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGccc                                                                                               <  1:1263513/150‑1 (MQ=255)
                                                        cAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTCAGCCCTa                                                                                             >  1:1430826/1‑150 (MQ=255)
                                                        cAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCtt                                                                                             >  2:597523/1‑149 (MQ=255)
                                                        cAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTACAGCGTCTCATTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGcccaa                                                                                             >  1:1422703/1‑148 (MQ=255)
                                                           ggTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCTCGACCAAAACGAAAATAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTg                                                                                          <  2:128151/150‑1 (MQ=255)
                                                           ggTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTg                                                                                          <  2:133424/150‑1 (MQ=255)
                                                            gTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTgg                                                                                         >  2:698124/1‑150 (MQ=255)
                                                              aCCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGc                                                                                       <  1:1670501/150‑1 (MQ=255)
                                                              aCCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGc                                                                                       <  2:821376/150‑1 (MQ=255)
                                                              aCCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGc                                                                                       <  1:402202/150‑1 (MQ=255)
                                                                cAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCAc                                                                                     <  1:98105/150‑1 (MQ=255)
                                                                  gCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTa                                                                                   >  1:1666924/1‑150 (MQ=255)
                                                                    tACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTacc                                                                                 <  2:240496/150‑1 (MQ=255)
                                                                    tACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTacc                                                                                 <  2:1625099/150‑1 (MQ=255)
                                                                     aCGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccc                                                                                >  2:848722/1‑149 (MQ=255)
                                                                      cGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccac                                                                               <  2:552303/150‑1 (MQ=255)
                                                                      cGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccac                                                                               >  1:1714383/1‑150 (MQ=255)
                                                                      cGAGTAAAGCAACTGGACGAGATACAGAAACCTGACAACCATTCCTCAGCCCAGGACCAAAACGAAAAAAGACGCTTTTCGGCGTCTCTTTTCAGGAATATGGTACCGAGCACGGAACTTGCACCCCTCCGCCCTCATGGGCACTaccac                                                                               >  1:1125192/1‑150 (MQ=255)
                                                                       taGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccacc                                                                              <  1:457212/149‑1 (MQ=255)
                                                                       gAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccacc                                                                              <  1:331051/150‑1 (MQ=255)
                                                                        aGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGAACCGAGGACGGGACTTGCACCCGTAAGCCCTATTGGGCACTACCACCt                                                                             >  2:124407/1‑150 (MQ=255)
                                                                           aaaGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTGTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCaa                                                                          >  2:477449/1‑150 (MQ=255)
                                                                           aaaGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCaa                                                                          >  2:731720/1‑150 (MQ=255)
                                                                           aaaGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCaa                                                                          >  2:482917/1‑150 (MQ=255)
                                                                            aaGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGCGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAg                                                                         >  2:179418/1‑150 (MQ=255)
                                                                               cAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTa                                                                      <  1:345967/150‑1 (MQ=255)
                                                                                  cTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCg                                                                   <  1:394031/150‑1 (MQ=255)
                                                                                    ggACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCgtg                                                                 >  1:1487836/1‑150 (MQ=255)
                                                                                     gACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTTAACCCGTAAGCCCTATAGGGCACTACCACCTCAAGGTAGCgtgt                                                                >  2:1056828/1‑150 (MQ=255)
                                                                                       cGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCt                                                              <  2:1092386/150‑1 (MQ=255)
                                                                                         agatacagatacCTGACAACCATTCCTCAGACCAGTACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAc                                                            <  1:77917/150‑1 (MQ=255)
                                                                                         agatacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAc                                                            <  2:508835/150‑1 (MQ=255)
                                                                                          gatacagatacCTGACAACCATTCCTCAGACCAGGACCATAACGAAAAAAGACGCGTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTTGGCACTACCACCTCAAGGTAGCGTGTCTAcc                                                           <  2:704284/150‑1 (MQ=255)
                                                                                          gatacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACACGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAcc                                                           >  1:301176/1‑150 (MQ=255)
                                                                                          gatacagatacCTGACAACCATTCCTCAGACCAGGAACAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTATGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAcc                                                           <  2:760523/150‑1 (MQ=255)
                                                                                          gatacagatacCTGACAACCAATCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAATCCGTAAGCCCTATTGGGCACTAACACCTCAAGGTAGCGTGTCTAcc                                                           <  2:1394311/150‑1 (MQ=255)
                                                                                           atacagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCa                                                          <  1:509870/150‑1 (MQ=255)
                                                                                             acagatacCTGACAACCATTCCTCAGACCAGGACCAAACCGAAAAAAGACGCTATTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGTACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAt                                                        <  1:1093190/150‑1 (MQ=255)
                                                                                             acagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAt                                                        <  1:1523202/150‑1 (MQ=255)
                                                                                              cagatacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAtt                                                       >  1:251756/1‑150 (MQ=255)
                                                                                               agatacCTGACAACCATTCATCAGACCAGGACCAAAACGAAAAAAGACGCCTTTCAGCGTCTCTTTTAAGGAATTTGGTACCGAGGACGGGACTACAACCCGTAAGACCTAGTGGGCACTAACACCTCAAGGTAGCGTGTCTAGCAAATc                                                      >  1:1082967/1‑150 (MQ=255)
                                                                                                gatacCTGACAACCTTTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTcc                                                     <  1:188472/150‑1 (MQ=255)
                                                                                                  tacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCTAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccac                                                   >  1:1305484/1‑150 (MQ=255)
                                                                                                  tacCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccac                                                   <  1:698124/150‑1 (MQ=255)
                                                                                                    ccTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGGGTCTACCAATAccacca                                                 >  2:1228565/1‑150 (MQ=255)
                                                                                                      tGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCaccacc                                               >  2:1193641/1‑150 (MQ=255)
                                                                                                       taCAACCATTACTCCGAACAGGACCAAAAATAAAAAAGCAGCTTTTCAGAGTCTCTTTTCTTGAATTTGGTACCGAGGACGGTACTTGAACCCGGAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCt                                              <  2:954599/149‑1 (MQ=255)
                                                                                                       gACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCaccaccg                                              >  2:583817/1‑149 (MQ=255)
                                                                                                         cAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCCCCTCg                                            >  1:715390/1‑150 (MQ=255)
                                                                                                           aCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCCCCTCGGc                                          >  1:402645/1‑150 (MQ=255)
                                                                                                           aCCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGc                                          <  1:1354772/150‑1 (MQ=255)
                                                                                                           aCCAATCCTCAGACCAGGACCAAAACGAATACAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGCTCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGc                                          <  2:119862/150‑1 (MQ=255)
                                                                                                                 ccTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGAt                                    >  1:1049348/1‑150 (MQ=255)
                                                                                                                   tCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGAtac                                  <  2:757167/150‑1 (MQ=255)
                                                                                                                       aCCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCCCCACCTCGGCACGGATactact                              >  2:1014056/1‑150 (MQ=255)
                                                                                                                        ccAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACtt                             >  1:339180/1‑150 (MQ=255)
                                                                                                                           ggACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACttttt                          >  1:536442/1‑150 (MQ=255)
                                                                                                                           ggACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACttttt                          >  2:1404525/1‑150 (MQ=255)
                                                                                                                            caCCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTa                         <  2:360099/149‑1 (MQ=255)
                                                                                                                             aCCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAg                        >  1:597902/1‑150 (MQ=255)
                                                                                                                               cAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGtt                      >  1:768201/1‑150 (MQ=255)
                                                                                                                                   cagAAAAGAGACGCTGAAAAGCGTCTTTTTTCTGTAATTTGGTACCGAGGACGGTACTTGAACCCGTAAGCCCTATTGGGCACTACCACATCAAGGTAGCTATTCTACCAATACCACCACCTCGCCACGGATACAACTGTTTAGTTCtgg                  >  2:277436/3‑150 (MQ=255)
                                                                                                                                    agAAAAGAGACGCTGAAAAGCGTCTTTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGa                 >  2:1186350/2‑150 (MQ=255)
                                                                                                                                     gAAAAGAGACGCTGAAAAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCGCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAACAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGat                >  1:1046640/1‑150 (MQ=255)
                                                                                                                                       aaaaaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGatat              <  2:114514/150‑1 (MQ=255)
                                                                                                                                        aaaaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATc             >  2:1232665/1‑150 (MQ=255)
                                                                                                                                          aaGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGTCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGc           <  1:245247/150‑1 (MQ=255)
                                                                                                                                          aaGACGCTTTTCAGCGTCTCGTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTTTCTACCAATTCCACCACCTCGGCACGGATACAACTTTTTAGTTCGGGATATCGc           >  1:1062882/1‑150 (MQ=255)
                                                                                                                                             aCGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTgg        >  2:713960/1‑150 (MQ=255)
                                                                                                                                              cGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGt       >  2:839497/1‑150 (MQ=255)
                                                                                                                                                  tttCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGc   >  1:454595/1‑150 (MQ=255)
                                                                                                                                                  tttCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGc   <  2:1159264/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGCT  >  NC_000913/3321902‑3322198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: