Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,324,956 C→T 8.3% intergenic (‑45/+45) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,324,9560CT 8.3% 360.4 / 12.3 119intergenic (‑45/+45)folP/ftsH7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
Reads supporting (aligned to +/- strand):  ref base C (56/53);  new base T (5/5);  total (61/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

GTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTT  >  NC_000913/3324807‑3325105
                                                                                                                                                     |                                                                                                                                                     
gTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCAc                                                                                                                                                       >  1:491726/1‑150 (MQ=255)
 ttATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACg                                                                                                                                                      <  1:1116834/150‑1 (MQ=255)
 ttATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACg                                                                                                                                                      <  1:3741/150‑1 (MQ=255)
 ttATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACg                                                                                                                                                      >  1:276675/1‑150 (MQ=255)
  tATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGc                                                                                                                                                     >  1:965064/1‑150 (MQ=255)
  tATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGGTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGc                                                                                                                                                     <  2:772159/150‑1 (MQ=255)
       gTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCgg                                                                                                                                                >  1:289247/1‑150 (MQ=255)
        tGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCCTTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCggg                                                                                                                                               >  1:737479/1‑150 (MQ=255)
        tGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCggg                                                                                                                                               <  1:684494/150‑1 (MQ=255)
         gCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCgggg                                                                                                                                              <  2:388529/150‑1 (MQ=255)
         gCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTGTCATGATGGTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCgggg                                                                                                                                              <  1:1665601/150‑1 (MQ=255)
         gCCACCATCCAAAAATTAATCAGTCGTGACGTTGAGTATCACCATTACGTTAGGATGGCTAAGGTCCAGTGAAGTTCCCTGGGCAAAGAGTTTCATGATGTTATCCCTTGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCgggg                                                                                                                                              <  1:1012581/150‑1 (MQ=255)
          ccaccaTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGt                                                                                                                                             >  1:1615562/1‑150 (MQ=255)
           caccaTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGtt                                                                                                                                            <  1:1347145/150‑1 (MQ=255)
           caccaTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCAAGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGtt                                                                                                                                            >  2:61869/1‑150 (MQ=255)
              caTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGttttt                                                                                                                                         <  2:1020883/150‑2 (MQ=255)
                tCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGttttttc                                                                                                                                       <  1:494342/150‑4 (MQ=255)
                 ccGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGt                                                                                                                                      >  1:1370296/1‑150 (MQ=255)
                 ccGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGt                                                                                                                                      <  1:820220/150‑1 (MQ=255)
                 ccGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGACCAGTGAAGAACCCTGGGCACAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACAACGGAGCACGCCCCGGGGTTTTCGGt                                                                                                                                      >  1:1061230/1‑150 (MQ=255)
                   gAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTAc                                                                                                                                    >  2:1262296/1‑150 (MQ=255)
                   gAAAAGGAATCAGGCGCGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGGTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTAc                                                                                                                                    <  1:1368937/150‑1 (MQ=255)
                      aaGGAATCAGGCGTGACGTTGAGGATACCCATTACGTGAGCATGGCTAAGGTCCAGTGAAGTTCCCTGGGCAAAGAGATTCAAGATGTAATCCCTGGTATGAATTGATAAGAAAAAACACCGGAGCACGCCACGGCGATTTCGGTACaaa                                                                                                                                 >  2:1582199/1‑150 (MQ=255)
                           aTCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAg                                                                                                                            <  1:83336/150‑1 (MQ=255)
                           aTCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGGCCAGTGAAGTACCCTGGGCAAAGAGTATCATGATGTTATCCCTGGTATGAATTGATAAGAAACAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAg                                                                                                                            <  1:160285/150‑1 (MQ=255)
                             cAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGTGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTc                                                                                                                          >  2:947357/1‑150 (MQ=255)
                                gCGTGACGTTGAGGATCCCCAATACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGAGGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCAtg                                                                                                                       >  2:650934/1‑149 (MQ=255)
                                 cGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGCGTTTTCGGTACAAATAAAGTCATca                                                                                                                      >  2:863481/1‑149 (MQ=255)
                                         tGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCggg                                                                                                              <  2:382908/150‑1 (MQ=255)
                                            ggATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAac                                                                                                           <  1:1149737/150‑1 (MQ=255)
                                               tCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCAGGGGTTTTCGGTACAAATAAAGTCATCTGATGCGGGAactta                                                                                                        >  1:1240948/1‑150 (MQ=255)
                                                  ccATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAacttactt                                                                                                     <  2:790735/150‑1 (MQ=255)
                                                     ttACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTc                                                                                                  <  1:1560907/150‑1 (MQ=255)
                                                        cGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGcc                                                                                               <  2:1440726/150‑1 (MQ=255)
                                                         gTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCt                                                                                              >  1:1081436/1‑150 (MQ=255)
                                                                tGGCTAAGGTCCAGTGCAGTACCGTTGGCAAAGTGTTGCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGct                                                                                       <  2:313324/150‑1 (MQ=255)
                                                                 ggCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGctc                                                                                      <  1:1683545/150‑1 (MQ=255)
                                                                  gCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGctct                                                                                     >  2:654600/1‑150 (MQ=255)
                                                                  gCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGctct                                                                                     >  1:86939/1‑150 (MQ=255)
                                                                  gCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTAATCTGATTCGGGAACTTACTTGTCGCCTAACTGctct                                                                                     >  1:1029023/1‑150 (MQ=255)
                                                                  gCTAAGGTCCAGTGAAGCACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGctct                                                                                     >  1:31848/1‑150 (MQ=255)
                                                                   cTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTAGCCCGGGTATGAATTGATAAGAAAAAACCCCGGAGCCCTCCCCGGGGTTTTCGGTACAAATACAGTCATCTGAGGCGGGACCTTACTTGTCGCCTAACTGCTCTg                                                                                    >  2:168490/1‑150 (MQ=255)
                                                                   cTAAGGTCCAGTGAAGTACCCTCGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTg                                                                                    <  1:61412/150‑1 (MQ=255)
                                                                       ggTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACAt                                                                                >  2:35568/1‑150 (MQ=255)
                                                                       ggTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACAt                                                                                >  2:879869/1‑150 (MQ=255)
                                                                         tCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATgg                                                                              >  1:560895/1‑150 (MQ=255)
                                                                          ccAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgt                                                                             <  2:86939/150‑1 (MQ=255)
                                                                           cAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgtg                                                                            <  1:1003020/150‑1 (MQ=255)
                                                                            aGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCCCGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGAAGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgtgt                                                                           >  2:1638070/1‑150 (MQ=255)
                                                                             gTGAAGTACCCTGGGCAAAGAGTTTCATTATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGtt                                                                          <  2:1477928/150‑1 (MQ=255)
                                                                               gAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTTATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTAc                                                                        >  2:1722269/1‑150 (MQ=255)
                                                                               gAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTAc                                                                        >  2:365940/1‑150 (MQ=255)
                                                                                    aCCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCggg                                                                   >  2:1283621/1‑150 (MQ=255)
                                                                                     cccTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAACAACCCCGGAGCACGACACGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGt                                                                  >  1:821342/1‑150 (MQ=255)
                                                                                         gggCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCgg                                                              <  2:276675/150‑1 (MQ=255)
                                                                                         gggCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCgg                                                              <  2:773973/150‑1 (MQ=255)
                                                                                          ggCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAATCCCCGGAGCCCGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGc                                                             <  1:161502/150‑1 (MQ=255)
                                                                                          ggCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGc                                                             >  2:1690004/1‑150 (MQ=255)
                                                                                              aaGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTAc                                                         <  2:1214347/150‑1 (MQ=255)
                                                                                                  gTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCgg                                                     >  2:1501582/1‑150 (MQ=255)
                                                                                                  gTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCgg                                                     <  2:165988/150‑1 (MQ=255)
                                                                                                  gTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCgg                                                     >  1:1203382/1‑150 (MQ=255)
                                                                                                   tttCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGt                                                    >  1:716167/1‑150 (MQ=255)
                                                                                                     tCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTtc                                                  <  2:1548545/150‑1 (MQ=255)
                                                                                                      cATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTtca                                                 <  1:879869/150‑1 (MQ=255)
                                                                                                         gatgTTATCCCTGTTATGAATTGATAAGAAAAACCCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTtcatca                                              <  1:851624/150‑1 (MQ=255)
                                                                                                         gatgTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTtcatca                                              >  2:478706/1‑150 (MQ=255)
                                                                                                            gTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAAcc                                           <  1:1498614/150‑1 (MQ=255)
                                                                                                            gTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAAcc                                           <  2:1081436/150‑1 (MQ=255)
                                                                                                              tATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCgg                                         >  2:582412/1‑150 (MQ=255)
                                                                                                              tATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCgg                                         >  2:1721599/1‑150 (MQ=255)
                                                                                                              tATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGAACGCCACGTGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCACATAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCATCCgg                                         >  2:109440/1‑150 (MQ=255)
                                                                                                                tCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGAc                                       <  2:1601094/150‑1 (MQ=255)
                                                                                                                tCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGAc                                       <  2:1182582/150‑1 (MQ=255)
                                                                                                                  ccTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACACGGGTTCGGCGTACGCGGTTCATCAACCGGACga                                     >  2:1303401/1‑150 (MQ=255)
                                                                                                                      gTATGAATTTATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACgaggag                                 <  1:1702193/150‑1 (MQ=255)
                                                                                                                       tATGAATTGATAAGAAAAAACCCCTGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGTGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGc                                <  2:1372140/150‑1 (MQ=255)
                                                                                                                       tATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGc                                <  1:1262296/150‑1 (MQ=255)
                                                                                                                        aTGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGcc                               >  1:1263093/1‑150 (MQ=255)
                                                                                                                         tGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCGGGGTTCGGCGTACGCGGTTGTTCAGCCGGACGAGGAGGCt                              >  1:299561/1‑150 (MQ=255)
                                                                                                                         tGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCt                              <  2:520216/150‑1 (MQ=255)
                                                                                                                             tttataataAAAAACCCCGCAGCACGCCCCGGGTCTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTTTTACCCGTGATCTGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTgg                          <  1:329964/147‑1 (MQ=255)
                                                                                                                              tGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTggc                         >  1:1677172/1‑149 (MQ=255)
                                                                                                                                   aGAAAAAACCCCGTAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTAcc                    <  2:1274452/150‑1 (MQ=255)
                                                                                                                                   aGAAAAAACCCCGGAGCACGCCGCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTCCTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTGCGGCGGACGCGGGTCCTCACCCGGTCGAGGAGCCTTTGGAGTAcc                    >  1:285869/1‑150 (MQ=255)
                                                                                                                                   aGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTAcc                    >  2:1275612/1‑150 (MQ=255)
                                                                                                                                    gAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGGCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCACCCGGACGAGGAGCCTTTGGACTACCa                   >  2:303376/1‑150 (MQ=255)
                                                                                                                                    ccgaaaaCCCCGGGGCGTGCTCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAAGTGACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGGTCATCAACCGGACGAGGAGCCTTTGGACTACCa                   >  1:906690/4‑150 (MQ=255)
                                                                                                                                      gaaaaCCCCGGGGCGTGCTCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCAtt                 >  1:1249570/2‑150 (MQ=255)
                                                                                                                                       aaaaCCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGCCTACCATTg                >  1:1209595/1‑150 (MQ=255)
                                                                                                                                         aaCCCCGGGGCGTGCTCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTc              >  1:544184/1‑150 (MQ=255)
                                                                                                                                          aCCCCGGGGCGTGCTCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCg             >  2:803348/1‑150 (MQ=255)
                                                                                                                                          aCCCCGGGGCGTGCTCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCg             >  2:1114932/1‑150 (MQ=255)
                                                                                                                                           ccccGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGc            >  2:493009/1‑150 (MQ=255)
                                                                                                                                            cccGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGcc           >  1:729656/1‑150 (MQ=255)
                                                                                                                                            cccGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGcc           >  2:684421/1‑150 (MQ=255)
                                                                                                                                               ggAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTAACTAGTCGCCTACCTGCTCTGACATGGGGTTACCCGGGTTCGGCGTACGCGGGGCATCAACCTGACGAGGAGCCTTTGGACTACCATTGTCGCCAGa        <  1:650934/150‑1 (MQ=255)
                                                                                                                                                    ccGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTGCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGt   <  2:1615562/149‑1 (MQ=255)
                                                                                                                                                     cGCCCCGGGGTTTTCGGTTCATATACTGTCTTCTGATGCGGGAACTTTCTGGTCGCCTAAGTGCTCTGACATGGTGTTTTGCGGGTGCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTTGACTACCATTGTCGCCAGAATTGtt  <  2:1061230/150‑1 (MQ=255)
                                                                                                                                                     cGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGtt  <  2:1167009/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTATGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTT  >  NC_000913/3324807‑3325105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: