Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,324,959 C→T 8.4% intergenic (‑48/+42) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,324,9590CT 8.4% 351.5 / 14.2 118intergenic (‑48/+42)folP/ftsH7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
Reads supporting (aligned to +/- strand):  ref base C (54/54);  new base T (5/5);  total (59/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01

Only 100 of 119 total aligned reads displayed.

CGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTA  >  NC_000913/3324813‑3325106
                                                                                                                                                  |                                                                                                                                                   
cGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCg                                                                                                                                                  >  1:16528/1‑150 (MQ=255)
  tGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCCTTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCggg                                                                                                                                                >  1:737479/1‑150 (MQ=255)
   gCCACCATCCGAAAAGGAATCAGGCGTGACGTTGATGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCgggg                                                                                                                                               <  1:868745/150‑1 (MQ=255)
   gCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCgggg                                                                                                                                               <  2:388529/150‑1 (MQ=255)
   gCCACCATCCAAAAATTAATCAGTCGTGACGTTGAGTATCACCATTACGTTAGGATGGCTAAGGTCCAGTGAAGTTCCCTGGGCAAAGAGTTTCATGATGTTATCCCTTGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCgggg                                                                                                                                               <  1:1012581/150‑1 (MQ=255)
    ccaccaTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGt                                                                                                                                              >  1:1615562/1‑150 (MQ=255)
     caccaTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGtt                                                                                                                                             <  1:1347145/150‑1 (MQ=255)
     caccaTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCAAGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGtt                                                                                                                                             >  2:61869/1‑150 (MQ=255)
        caTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGttttt                                                                                                                                          <  2:1020883/150‑2 (MQ=255)
          tCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGttttttc                                                                                                                                        <  1:494342/150‑4 (MQ=255)
           cctaaaaGGACTCAGGCGTGACGTTGAGTCTCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAACTACCCTCGGCAAACAGTTTCATGATGTTATCCCGGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGttttttct                                                                                                                                       <  1:1094407/147‑5 (MQ=255)
           ccGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGt                                                                                                                                       <  1:820220/150‑1 (MQ=255)
           ccGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGt                                                                                                                                       >  1:1370296/1‑150 (MQ=255)
           ccGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGACCAGTGAAGAACCCTGGGCACAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACAACGGAGCACGCCCCGGGGTTTTCGGt                                                                                                                                       >  1:1061230/1‑150 (MQ=255)
             gAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTAc                                                                                                                                     <  1:1239140/150‑1 (MQ=255)
             gAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTAc                                                                                                                                     >  2:1262296/1‑150 (MQ=255)
             gAAAAGGAATCAGGCGCGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGGTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTAc                                                                                                                                     <  1:1368937/150‑1 (MQ=255)
                aaGGAATCAGGCGTGACGTTGAGGATACCCATTACGTGAGCATGGCTAAGGTCCAGTGAAGTTCCCTGGGCAAAGAGATTCAAGATGTAATCCCTGGTATGAATTGATAAGAAAAAACACCGGAGCACGCCACGGCGATTTCGGTACaaa                                                                                                                                  >  2:1582199/1‑150 (MQ=255)
                  ggAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATa                                                                                                                                <  2:32949/150‑1 (MQ=255)
                     aTCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGGCCAGTGAAGTACCCTGGGCAAAGAGTATCATGATGTTATCCCTGGTATGAATTGATAAGAAACAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAg                                                                                                                             <  1:160285/150‑1 (MQ=255)
                       cAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGTGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTc                                                                                                                           >  2:947357/1‑150 (MQ=255)
                       cAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTc                                                                                                                           <  1:133488/150‑1 (MQ=255)
                          gCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATc                                                                                                                        <  2:1131017/150‑1 (MQ=255)
                           cGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGCGTTTTCGGTACAAATAAAGTCATca                                                                                                                       >  2:863481/1‑149 (MQ=255)
                                 gTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCg                                                                                                                 >  2:604572/1‑150 (MQ=255)
                                   tGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCggg                                                                                                               <  2:382908/150‑1 (MQ=255)
                                      ggATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAac                                                                                                            <  2:816136/150‑1 (MQ=255)
                                         tCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCAGGGGTTTTCGGTACAAATAAAGTCATCTGATGCGGGAactta                                                                                                         >  1:1240948/1‑150 (MQ=255)
                                            ccATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAacttactt                                                                                                      <  2:790735/150‑1 (MQ=255)
                                               ttACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTc                                                                                                   <  1:1560907/150‑1 (MQ=255)
                                                   gTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCt                                                                                               >  1:1081436/1‑150 (MQ=255)
                                                           ggCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGctc                                                                                       <  1:1683545/150‑1 (MQ=255)
                                                            gCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGctct                                                                                      >  2:654600/1‑150 (MQ=255)
                                                            gCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGctct                                                                                      >  1:86939/1‑150 (MQ=255)
                                                            gCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTAATCTGATTCGGGAACTTACTTGTCGCCTAACTGctct                                                                                      >  1:1029023/1‑150 (MQ=255)
                                                            gCTAAGGTCCAGTGAAGCACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGctct                                                                                      >  1:31848/1‑150 (MQ=255)
                                                             gtAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTg                                                                                     <  2:755695/149‑1 (MQ=255)
                                                             cTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTg                                                                                     >  2:1519637/1‑150 (MQ=255)
                                                             cTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTg                                                                                     >  2:1691248/1‑150 (MQ=255)
                                                             cTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTAGCCCGGGTATGAATTGATAAGAAAAAACCCCGGAGCCCTCCCCGGGGTTTTCGGTACAAATACAGTCATCTGAGGCGGGACCTTACTTGTCGCCTAACTGCTCTg                                                                                     >  2:168490/1‑150 (MQ=255)
                                                             cTAAGGTCCAGTGAAGTACCCTCGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTg                                                                                     <  1:61412/150‑1 (MQ=255)
                                                                 ggTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACAt                                                                                 >  2:879869/1‑150 (MQ=255)
                                                                 ggTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACAt                                                                                 >  2:35568/1‑150 (MQ=255)
                                                                   tCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATgg                                                                               >  1:560895/1‑150 (MQ=255)
                                                                    ccAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgt                                                                              <  2:86939/150‑1 (MQ=255)
                                                                     cAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgtg                                                                             <  1:1003020/150‑1 (MQ=255)
                                                                      aGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCCCGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGAAGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgtgt                                                                            >  2:1638070/1‑150 (MQ=255)
                                                                       gTGAAGTACCCTGGGCAAAGAGTTTCATTATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGtt                                                                           <  2:1477928/150‑1 (MQ=255)
                                                                         gAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTTATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTAc                                                                         >  2:1722269/1‑150 (MQ=255)
                                                                         gAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTAc                                                                         >  2:365940/1‑150 (MQ=255)
                                                                              aCCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCggg                                                                    >  2:1283621/1‑150 (MQ=255)
                                                                              aCCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCggg                                                                    <  1:986115/150‑1 (MQ=255)
                                                                               cccTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAACAACCCCGGAGCACGACACGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGt                                                                   >  1:821342/1‑150 (MQ=255)
                                                                                   gggCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCgg                                                               <  2:773973/150‑1 (MQ=255)
                                                                                   gggCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCgg                                                               <  2:276675/150‑1 (MQ=255)
                                                                                   gggCAAAGAGTTTCATGATGTTATCCCTGGTATCAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCgg                                                               <  2:162099/150‑1 (MQ=255)
                                                                                    ggCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAATCCCCGGAGCCCGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGc                                                              <  1:161502/150‑1 (MQ=255)
                                                                                      cAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGt                                                            >  1:1544280/1‑150 (MQ=255)
                                                                                        aaGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTAc                                                          <  2:1214347/150‑1 (MQ=255)
                                                                                            gTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCgg                                                      >  1:1203382/1‑150 (MQ=255)
                                                                                            gTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCgg                                                      >  2:1501582/1‑150 (MQ=255)
                                                                                            gTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCgg                                                      <  2:165988/150‑1 (MQ=255)
                                                                                             tttCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGt                                                     >  1:716167/1‑150 (MQ=255)
                                                                                               tCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTtc                                                   <  1:798745/150‑1 (MQ=255)
                                                                                               tCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTtc                                                   <  2:1548545/150‑1 (MQ=255)
                                                                                                cATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTtca                                                  <  1:879869/150‑1 (MQ=255)
                                                                                                   gatgTTATCCCTGTTATGAATTGATAAGAAAAACCCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTtcatca                                               <  1:851624/150‑1 (MQ=255)
                                                                                                      gTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAAcc                                            <  2:1081436/150‑1 (MQ=255)
                                                                                                        tATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCgg                                          >  2:582412/1‑150 (MQ=255)
                                                                                                        tATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCgg                                          >  2:1721599/1‑150 (MQ=255)
                                                                                                        tATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGAACGCCACGTGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCACATAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCATCCgg                                          >  2:109440/1‑150 (MQ=255)
                                                                                                          tCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGAc                                        <  2:1182582/150‑1 (MQ=255)
                                                                                                            ccTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACACGGGTTCGGCGTACGCGGTTCATCAACCGGACga                                      >  2:1303401/1‑150 (MQ=255)
                                                                                                                gTATGAATTTATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACgaggag                                  <  1:1702193/150‑1 (MQ=255)
                                                                                                                 tATGAATTGATAAGAAAAAACCCCTGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGTGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGc                                 <  2:1372140/150‑1 (MQ=255)
                                                                                                                  aTGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGcc                                >  1:1263093/1‑150 (MQ=255)
                                                                                                                   tGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCGGGGTTCGGCGTACGCGGTTGTTCAGCCGGACGAGGAGGCt                               >  1:299561/1‑150 (MQ=255)
                                                                                                                   tGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCt                               <  2:520216/150‑1 (MQ=255)
                                                                                                                    gAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACATATACAGTCATCTGCTGCGGGACCTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCACCCGGACGAGGAGCCtt                              >  1:67643/1‑150 (MQ=255)
                                                                                                                       tttataataAAAAACCCCGCAGCACGCCCCGGGTCTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTTTTACCCGTGATCTGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTgg                           <  1:329964/147‑1 (MQ=255)
                                                                                                                        tGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTggc                          >  1:1677172/1‑149 (MQ=255)
                                                                                                                             aGAAAAAACCCCGTAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTAcc                     <  2:1274452/150‑1 (MQ=255)
                                                                                                                             aGAAAAAACCCCGGAGCACGCCGCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTCCTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTGCGGCGGACGCGGGTCCTCACCCGGTCGAGGAGCCTTTGGAGTAcc                     >  1:285869/1‑150 (MQ=255)
                                                                                                                             aGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTAcc                     >  2:1275612/1‑150 (MQ=255)
                                                                                                                             aGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACGTACTTGTCGCCTAACTGCGCTGCCATGGTGTTACCCGGGTTCGGCGTCCGCGGTTCATCAACCGGACGAGGCGCCTGTGGACTAcc                     >  1:327099/1‑150 (MQ=255)
                                                                                                                              gAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCa                    <  2:393948/150‑1 (MQ=255)
                                                                                                                              gAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGGCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCACCCGGACGAGGAGCCTTTGGACTACCa                    >  2:303376/1‑150 (MQ=255)
                                                                                                                                gaaaaCCCCGGGGCGTGCTCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCAtt                  >  1:1249570/2‑150 (MQ=255)
                                                                                                                                 aaaaCCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGCCTACCATTg                 >  1:1209595/1‑150 (MQ=255)
                                                                                                                                   aaCCCCGGGGCGTGCTCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTc               >  1:544184/1‑150 (MQ=255)
                                                                                                                                     ccccGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGc             >  2:493009/1‑150 (MQ=255)
                                                                                                                                      cccGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGcc            >  2:684421/1‑150 (MQ=255)
                                                                                                                                      cccGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGcc            >  1:729656/1‑150 (MQ=255)
                                                                                                                                      cccGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGcc            >  1:252157/1‑150 (MQ=255)
                                                                                                                                         ggAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTAACTAGTCGCCTACCTGCTCTGACATGGGGTTACCCGGGTTCGGCGTACGCGGGGCATCAACCTGACGAGGAGCCTTTGGACTACCATTGTCGCCAGa         <  1:650934/150‑1 (MQ=255)
                                                                                                                                              ccGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTGCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGt    <  2:1615562/149‑1 (MQ=255)
                                                                                                                                               cGCCCCGGGGTTTTCGGTTCATATACTGTCTTCTGATGCGGGAACTTTCTGGTCGCCTAAGTGCTCTGACATGGTGTTTTGCGGGTGCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTTGACTACCATTGTCGCCAGAATTGtt   <  2:1061230/150‑1 (MQ=255)
                                                                                                                                               cGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGtt   <  2:1167009/150‑1 (MQ=255)
                                                                                                                                               cGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGtt   <  2:1370296/150‑1 (MQ=255)
                                                                                                                                                gacccGGGGTTTTCGGTACAAATACATTAATCTGATGCGGGAACTTACTTGTCGCCTAACTGATCTGACATGGTGTTAACCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTa  <  1:863481/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTA  >  NC_000913/3324813‑3325106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: