Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,334,705 C→T 8.0% intergenic (+24/‑204) ispB → / → sfsB octaprenyl diphosphate synthase/malPQ operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,334,7050CT 8.0% 334.3 / 12.5 117intergenic (+24/‑204)ispB/sfsBoctaprenyl diphosphate synthase/malPQ operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (53/53);  new base T (5/4);  total (59/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.79e-01

Only 100 of 121 total aligned reads displayed.

GACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTAT  >  NC_000913/3334558‑3334847
                                                                                                                                                   |                                                                                                                                              
gACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAgcgc                                                                                                                                              >  2:1029707/1‑150 (MQ=255)
gACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTAGGCGAGAAGCACTCATCGGCCTCGCGCACATCTCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAccgc                                                                                                                                              >  1:232338/1‑150 (MQ=255)
 aCGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCCGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAgcgcg                                                                                                                                             <  2:1022746/150‑1 (MQ=255)
  cgcgTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCgggcgc                                                                                                                                            >  1:1459533/1‑150 (MQ=255)
    cgTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATAGTTAATCCCCTCCCCACATCCCGCGCAGCCCGCgg                                                                                                                                          >  2:55491/1‑150 (MQ=255)
       cAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCGCTGCGCGGGAt                                                                                                                                       <  1:789531/150‑1 (MQ=255)
        aGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCGCTGCGCGGGATg                                                                                                                                      <  2:266422/150‑1 (MQ=255)
        aGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATg                                                                                                                                      <  1:1003960/150‑1 (MQ=255)
         gCGTGCCGAGTAAGAAGCAGACACAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATgg                                                                                                                                     <  1:1325404/150‑1 (MQ=255)
          cGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACGGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGt                                                                                                                                    >  2:1589877/1‑150 (MQ=255)
           gTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCGCTGCGCGGGATgagg                                                                                                                                   <  1:556004/150‑4 (MQ=255)
           gTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGtt                                                                                                                                   <  2:1311494/150‑1 (MQ=255)
            tGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTc                                                                                                                                  <  2:607643/150‑1 (MQ=255)
            tGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGGGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTc                                                                                                                                  <  1:333875/150‑1 (MQ=255)
            ggCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCGCTGCGCGGGATgaggg                                                                                                                                  <  2:49582/149‑5 (MQ=255)
             gCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGCCACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACAGCGCTGGTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTcc                                                                                                                                 <  1:225597/150‑1 (MQ=255)
               cGAGGAAGAAGCAGACAAAGCCCTCGCAGCGTTACAGGTGCTCCCGGACACCCCTAGGCGAGAAGCACGCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAAGCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAg                                                                                                                               <  1:822374/150‑1 (MQ=255)
                     agaagCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGtt                                                                                                                         >  2:492278/1‑150 (MQ=255)
                        agCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGAGGGGGCCAGGAAGTTccg                                                                                                                      >  1:1474247/1‑149 (MQ=255)
                        agCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCAGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGCTAGGGTCCAGTAAGTACCa                                                                                                                      >  1:1703813/1‑150 (MQ=255)
                         gCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTccct                                                                                                                     >  2:1513999/1‑148 (MQ=255)
                         gCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCAt                                                                                                                     <  1:1522241/150‑1 (MQ=255)
                          cAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGTGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATa                                                                                                                    <  1:344072/150‑1 (MQ=255)
                          cAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATa                                                                                                                    >  1:624421/1‑150 (MQ=255)
                           aGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCCGCGCCCGCGGGATGGTTCCAGTAAGTACCATaa                                                                                                                   >  2:1604604/1‑150 (MQ=255)
                             aCAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATaaaa                                                                                                                 >  1:208767/1‑150 (MQ=255)
                             aCAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATaaaa                                                                                                                 <  2:1231182/150‑1 (MQ=255)
                                aaGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAaca                                                                                                              >  1:1262128/1‑150 (MQ=255)
                                  gCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGATGGGTCCAGTAAGTTGCATATAAacacg                                                                                                            >  1:610600/1‑149 (MQ=255)
                                       cGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTc                                                                                                       <  1:1300500/150‑1 (MQ=255)
                                        gCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCa                                                                                                      <  2:1399233/150‑1 (MQ=255)
                                        gCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCa                                                                                                      <  2:1363930/150‑1 (MQ=255)
                                        gCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCa                                                                                                      <  1:937033/150‑1 (MQ=255)
                                        gCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCACCTCCCCTCATCCCGCGCCGCGCGCGGGTTGGTTGCAGTAAGTTGCATTAAAACACTTAttgg                                                                                                      >  1:859103/1‑148 (MQ=255)
                                           gCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTACTCCCCTCCCCTCATCCCGCGCCCCGCGCGGGTTGGTTACAGGTAGTTGCAAAAAAACACAGATTGAGcg                                                                                                   >  1:1524879/1‑149 (MQ=255)
                                             gTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGctct                                                                                                 <  1:341754/150‑1 (MQ=255)
                                                aCAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCtata                                                                                              <  2:859215/150‑1 (MQ=255)
                                                aCAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTGCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCtata                                                                                              <  1:1682516/150‑1 (MQ=255)
                                                   ggTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGATGGTTCCAGGAAGGTCCATAAAAACACTTATTCCGCGCTATAacc                                                                                           >  1:574584/1‑149 (MQ=255)
                                                      gCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCt                                                                                        >  2:1382177/1‑150 (MQ=255)
                                                       cTCCCTGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTg                                                                                       <  2:1094303/150‑1 (MQ=255)
                                                       cTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTg                                                                                       >  2:23228/1‑150 (MQ=255)
                                                       cTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTg                                                                                       <  2:618643/150‑1 (MQ=255)
                                                            ggACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTAAATCCCCTCCCCTCATCCCGCGCAGCGCGAGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGaaaaa                                                                                  <  2:1021641/150‑1 (MQ=255)
                                                                 ccccTTGGCGAGAAGCCCTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTGAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGGGGGGTCGCGTAGGTTCCATAAAAACACTTATTGAGCTCGAGACCCCCTGAAAAATAggg                                                                             >  2:500812/1‑150 (MQ=255)
                                                                 ccccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAggg                                                                             >  2:596308/1‑150 (MQ=255)
                                                                  cccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCCCGCAGCGCGGGGGGTGGGTCCAGTAAGTTACATAAAAACACATATTCAGCTCGATAACACCGGAAAAATAgggt                                                                            >  1:1348524/1‑149 (MQ=255)
                                                                    cTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGAtg                                                                          <  2:687112/150‑1 (MQ=255)
                                                                        gCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCa                                                                      <  1:389662/150‑1 (MQ=255)
                                                                          gagaAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCTATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATc                                                                    <  1:596628/150‑1 (MQ=255)
                                                                            gaAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCtt                                                                  <  2:1436207/150‑1 (MQ=255)
                                                                            gaAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCtt                                                                  <  2:766283/150‑1 (MQ=255)
                                                                               gCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtat                                                               <  2:416711/150‑1 (MQ=255)
                                                                                cACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTGCAGTAAGTTCCATAAAAAAACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtata                                                              >  2:757708/1‑150 (MQ=255)
                                                                                     aTCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGTGCCGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACTCCTGAAAAATAGGGAGGTGCATCTTTATATATTc                                                         >  1:1280887/1‑150 (MQ=255)
                                                                                     aTCGGCCTCGCGCACAGCGCTGTTCCACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTc                                                         <  2:1258531/150‑1 (MQ=255)
                                                                                       cGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTg                                                       <  2:1702694/150‑1 (MQ=255)
                                                                                       cGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTg                                                       <  2:1194149/150‑1 (MQ=255)
                                                                                        ggCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGa                                                      >  1:245786/1‑150 (MQ=255)
                                                                                         gCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGCTGGTTCCAGTAAGTTCCATACCAACACTTATTCCGCTCTATAAGCACTGAAAAATAGGGATGTGCATCTTTATATATTCTGat                                                     >  1:1245614/1‑149 (MQ=255)
                                                                                         gCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACCCTTATTCAGCTCTATAACACCGCTATAATAGGGATGTGGATCTTTATATATTCTGaa                                                     >  2:140425/1‑150 (MQ=255)
                                                                                         gCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGATGCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGaa                                                     >  1:1074025/1‑150 (MQ=255)
                                                                                          ccTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAat                                                    <  2:1262128/150‑1 (MQ=255)
                                                                                             cgcgCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATAtt                                                 <  2:1293518/150‑1 (MQ=255)
                                                                                             cgcgCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATAtt                                                 <  2:868905/150‑1 (MQ=255)
                                                                                                  acaTCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCACCGCGCGGGATGGTTCCAGTAAGTTCAAAAAAAACACATATACAGCTCAATAACACCTGAAAAATAAGGATTTGCATATTTATATATTCTGAATATTaacaa                                            >  1:1693627/1‑149 (MQ=255)
                                                                                                     tCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCAATAAAAACACATATACAGCTAAATAACACCTGAAAAAAAGGGATGTGCTTCTTTATATATTATGAATATTAACAatct                                         >  1:413887/1‑150 (MQ=255)
                                                                                                      cGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCtt                                        >  2:1725023/1‑150 (MQ=255)
                                                                                                      cGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCGTTATATATTCTGAATATTCACACTCtt                                        >  1:1246080/1‑150 (MQ=255)
                                                                                                       gCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACGTATTCAGCTCTATAACACCGGAAAAAAAGGGATGTGCAACTTTATATATTCTGAATATTCACCCTCttt                                       >  2:1590912/1‑150 (MQ=255)
                                                                                                           ttCAACGCGATCGTTAATCCACTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCATCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAg                                   <  2:1096664/150‑1 (MQ=255)
                                                                                                            tCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAgg                                  <  2:297865/150‑1 (MQ=255)
                                                                                                             cAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTATGAATATTAACACTCTTTACAGGa                                 >  2:1729450/1‑150 (MQ=255)
                                                                                                               aCGCGATCGTTAATCCCATCCCCTAATCCCGCGCAGCGGGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTATATAACTCCTGAAAACTAGGGATGTGCATCTTTATATATTCTGAATATTAACACTCTTTACAGGaaa                               <  2:1597901/150‑2 (MQ=255)
                                                                                                                cgcgATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTACATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTAATATATTCTGAATATTCACACTCTTTACAGGAACt                              >  2:739746/1‑150 (MQ=255)
                                                                                                                  cgATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACttt                            >  2:166464/1‑150 (MQ=255)
                                                                                                                   gATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACtttt                           >  2:775248/1‑150 (MQ=255)
                                                                                                                      cGTTAATCCCCTCCCCTCATCCCGCGCGGCGGGCGGGGTGGTTCCAGTAAGGTGCATTAAAACACGTATTCAGGGGTATAAAACCAGTAAAATAGGGATGTTGATATTTTTATATTTTGAATATTCAAAATGTTTAAAGGAACttttttt                        >  2:882562/1‑148 (MQ=255)
                                                                                                                      cGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTAAGCTGTATAACACCTGAAAAATAGGGATGTTCATCTTTATATTATATGAATATTAACACTCTTTACAGGAACTTTTTag                        >  2:1045785/1‑150 (MQ=255)
                                                                                                                      cGTTAATCCCCTACCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTACATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTag                        <  2:242108/150‑1 (MQ=255)
                                                                                                                       gTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGGTGGTTGCAGTAAGTTGCATAAAAACCCTTTTTCAGCGCTATAACACCGGAAAAATAGGGTTGTGGATCTTTTTTTTTTATGAATTTTCACACTATTTACAGGATCTTTTTatt                       >  2:363682/1‑148 (MQ=255)
                                                                                                                        ttAATCCCCTCCCCTCATCCCGCGCAGCGCGCTGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTagag                      <  1:1139867/150‑1 (MQ=255)
                                                                                                                         tAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGc                     <  2:1468115/150‑1 (MQ=255)
                                                                                                                          aaTCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTCAATATTCACACACTTTACAGGAACTTTTTAGAGCa                    >  2:230600/1‑150 (MQ=255)
                                                                                                                           aTCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAATTTCCAAAAAAACACGTATTCAGCTGTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTTAATATTCACACTCTTTACAGGAAATTTTTAGATCaa                   >  1:1653254/1‑150 (MQ=255)
                                                                                                                           aTCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAGCACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCaa                   >  1:939286/1‑150 (MQ=255)
                                                                                                                             cccctcccctCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATa                 >  1:1248546/1‑150 (MQ=255)
                                                                                                                               cctcccctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAgg               <  1:1024324/150‑1 (MQ=255)
                                                                                                                               cctcccctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAgg               >  2:1251743/1‑150 (MQ=255)
                                                                                                                                 tcccctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGcc             <  1:1382177/150‑1 (MQ=255)
                                                                                                                                    cctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTTAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATc          <  1:848121/150‑1 (MQ=255)
                                                                                                                                    aaccATCCCGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATc          >  1:1414511/4‑150 (MQ=255)
                                                                                                                                      ccATCCCGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAg        >  1:1478603/2‑150 (MQ=255)
                                                                                                                                       cATCCCGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAgg       >  2:61032/1‑150 (MQ=255)
                                                                                                                                       cATCCCGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAgg       >  1:175534/1‑150 (MQ=255)
                                                                                                                                        aTCCCGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa      >  2:1038522/1‑150 (MQ=255)
                                                                                                                                        aTCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa      >  2:487648/1‑150 (MQ=255)
                                                                                                                                        aTCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa      >  1:959818/1‑150 (MQ=255)
                                                                                                                                          cccGCGAAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTAAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTTTATATTATGAATAGTCACACTCGTTACAGGCCCGTTTTAGAGCAATAGGACATCAGGAGt    >  2:93330/1‑150 (MQ=255)
                                                                                                                                            cgcgCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGtat  <  1:1029707/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
GACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTAT  >  NC_000913/3334558‑3334847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: