Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,354,537 C→G 8.1% intergenic (‑487/‑188) yhcC ← / → gltB putative Fe‑S oxidoreductase, Radical SAM superfamily protein/glutamate synthase, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,354,5370CG 8.1% 382.5 / 18.9 122intergenic (‑487/‑188)yhcC/gltBputative Fe‑S oxidoreductase, Radical SAM superfamily protein/glutamate synthase, large subunit
Reads supporting (aligned to +/- strand):  ref base C (50/61);  new base G (3/7);  total (54/68)
Fisher's exact test for biased strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 135 total aligned reads displayed.

AGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGG  >  NC_000913/3354388‑3354686
                                                                                                                                                     |                                                                                                                                                     
aGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGagcagc                                                                                                                                                       >  2:1022016/1‑150 (MQ=255)
aGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGagcagc                                                                                                                                                       >  2:646998/1‑150 (MQ=255)
 gCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGcc                                                                                                                                                      <  1:1467802/150‑1 (MQ=255)
   cTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCCTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGGCTg                                                                                                                                                    >  1:1529850/1‑150 (MQ=255)
     tAAAGATAAAATCCATTTTAATTTCATTAATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGTAGCTTTCTGGATGAGCAGCCTGCt                                                                                                                                                  <  1:1072697/150‑1 (MQ=255)
     tAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCt                                                                                                                                                  <  2:236657/150‑1 (MQ=255)
       aaGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATATCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCtca                                                                                                                                                <  1:759715/150‑1 (MQ=255)
       aaGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTAGCGCTAAAGCACATTTCAGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCtca                                                                                                                                                <  2:243756/150‑1 (MQ=255)
          ttAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTTCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGGCTGCtcatcc                                                                                                                                             <  2:920837/149‑2 (MQ=255)
          aTAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCtcatca                                                                                                                                             <  2:48899/150‑1 (MQ=255)
          aTAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCACTGGAAGCTTTCTGGATGAGCAGCCTGCtcatca                                                                                                                                             >  1:1284603/1‑150 (MQ=255)
           tAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAATCAAATTTCTGTACCAATAAGCTTTCCATTTGACCTGTATCAGCTTTCCCGATAAGTTTGAAATCCGCTGGAAGCTTTCTGGATGAGCAGGCTGCgcatcca                                                                                                                                            <  2:1154465/150‑3 (MQ=255)
           tAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCCGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGTGCAGGCTGCtcatcca                                                                                                                                            <  2:906030/150‑3 (MQ=255)
            aaaaTCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGGCTGCtcatccag                                                                                                                                           <  1:349658/150‑4 (MQ=255)
             aaaTCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGCCCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCatat                                                                                                                                          >  2:1543779/1‑150 (MQ=255)
              aaTCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGGCTGCtcatccagaa                                                                                                                                         <  1:22894/150‑6 (MQ=255)
               aTCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATAttt                                                                                                                                        <  1:1118001/150‑1 (MQ=255)
                  cATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATg                                                                                                                                     <  2:714044/150‑1 (MQ=255)
                   tttttAATTTCAGTCATTTAATATAGAATTTTGGGCTAAAGCACATTTCTGTACCAATCAGCTTGCCATTTGACCTGTATGAGCTTTCCCGATAAGTTGTAAATCCGCTGGAAGCTTTCTGGTTGAGCAGCCTGCTCAGCATATTTATGc                                                                                                                                    <  2:1077586/149‑1 (MQ=255)
                   aTTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGc                                                                                                                                    <  1:1205175/150‑1 (MQ=255)
                   aTTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACATGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGc                                                                                                                                    >  1:1423246/1‑150 (MQ=255)
                     tttAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTCTCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAg                                                                                                                                  <  1:881045/150‑1 (MQ=255)
                     tttAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAg                                                                                                                                  <  1:342828/150‑1 (MQ=255)
                     tttAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAg                                                                                                                                  <  1:1143507/150‑1 (MQ=255)
                     tttAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCGGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCac                                                                                                                                  <  1:881049/150‑2 (MQ=255)
                      ttAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGt                                                                                                                                 >  2:1415141/1‑150 (MQ=255)
                         aTTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAAt                                                                                                                              >  2:692673/1‑150 (MQ=255)
                           ttCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTg                                                                                                                            <  1:1008761/150‑1 (MQ=255)
                             cAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTgag                                                                                                                          <  1:54950/150‑1 (MQ=255)
                                tCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATc                                                                                                                       >  1:718723/1‑150 (MQ=255)
                                   tttAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATcccc                                                                                                                    <  1:1165753/150‑1 (MQ=255)
                                     taataaAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCctc                                                                                                                  >  1:623773/1‑150 (MQ=255)
                                      aataaAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCctct                                                                                                                 >  2:1458524/1‑150 (MQ=255)
                                      aataaAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCctct                                                                                                                 <  2:566596/150‑1 (MQ=255)
                                       ataaAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCtt                                                                                                                >  1:1214735/1‑150 (MQ=255)
                                        taaAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCACGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCCGCCTGCTCATCATATTTATACAGTAATTGAGATCCCCTCTTc                                                                                                               >  1:937794/1‑150 (MQ=255)
                                         aaaGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCa                                                                                                              >  1:1366611/1‑150 (MQ=255)
                                         aaaGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCa                                                                                                              >  1:1521503/1‑150 (MQ=255)
                                            gAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCg                                                                                                           <  1:240743/150‑1 (MQ=255)
                                            gAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCg                                                                                                           <  2:396219/150‑1 (MQ=255)
                                            gAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCg                                                                                                           <  2:648869/150‑1 (MQ=255)
                                            gAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCg                                                                                                           <  2:705170/150‑1 (MQ=255)
                                                  tGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTaa                                                                                                     <  2:874179/150‑1 (MQ=255)
                                                   gcgcTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAAc                                                                                                    >  2:213197/1‑150 (MQ=255)
                                                    cgcTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAAcc                                                                                                   <  1:267846/150‑1 (MQ=255)
                                                    cgcTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAAcc                                                                                                   <  2:438890/150‑1 (MQ=255)
                                                      cTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGa                                                                                                 >  2:1276255/1‑150 (MQ=255)
                                                      cTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGa                                                                                                 <  2:1710747/150‑1 (MQ=255)
                                                      cTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGa                                                                                                 >  2:356547/1‑150 (MQ=255)
                                                        aaaGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTTGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATg                                                                                               <  1:1396720/150‑1 (MQ=255)
                                                        aaaGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCGCCGTATTAACCGATg                                                                                               >  2:1573680/1‑150 (MQ=255)
                                                        aaaGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATg                                                                                               <  2:167860/150‑1 (MQ=255)
                                                        aaaGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTGTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATg                                                                                               >  2:1039973/1‑150 (MQ=255)
                                                          aGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTAATCATATTTATGCATTAATTGAGATACCCTCTTCACCGTATTAAACGATGct                                                                                             >  1:1570586/1‑149 (MQ=255)
                                                           gCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGa                                                                                            >  1:1128970/1‑150 (MQ=255)
                                                                tttCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAgg                                                                                       <  2:1383982/150‑1 (MQ=255)
                                                                   cTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGaca                                                                                    <  2:1610812/150‑1 (MQ=255)
                                                                        ccAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATCAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAg                                                                               >  1:927002/1‑150 (MQ=255)
                                                                           aTAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATCAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAgggg                                                                            <  2:1038327/150‑1 (MQ=255)
                                                                            tAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTGACCGATGCGAAAAGGACAACAAggggg                                                                           <  2:262600/150‑1 (MQ=255)
                                                                             aaGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGc                                                                          <  2:1153825/150‑1 (MQ=255)
                                                                               gCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGa                                                                        <  1:1648820/150‑1 (MQ=255)
                                                                                cTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGaa                                                                       >  2:154526/1‑150 (MQ=255)
                                                                                  tGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATg                                                                     >  1:214381/1‑150 (MQ=255)
                                                                                  tGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATGGAGATCCCGTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGATTg                                                                     <  1:304345/150‑1 (MQ=255)
                                                                                    ccATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCg                                                                   <  2:409425/150‑1 (MQ=255)
                                                                                      aattGACCTGTATCAGCTTTCCCGAAAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAg                                                                 <  2:275089/148‑1 (MQ=255)
                                                                                         tGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcg                                                              <  1:1719178/150‑1 (MQ=255)
                                                                                          gACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTAATCATATTTATGCAGTAATTGAGATCCCCTCTTCCCCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgc                                                             >  2:235430/1‑150 (MQ=255)
                                                                                           aCCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcg                                                            >  1:1230428/1‑150 (MQ=255)
                                                                                            cgtGTTTCAGCGTTCCCGATATGTTGGAAATCCGCTGGAAGCTTACTGGATGAGCAGCCTGCTCATCATATTTATCCATTAATTTAGATCCCCTTTTCACCGTATTAACCGATGCGAATAGGACACCAAGGGGCCGAATGGGAGgcgcgc                                                           <  2:1167390/148‑1 (MQ=255)
                                                                                               gTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTa                                                        <  2:223960/150‑1 (MQ=255)
                                                                                                 aTCAGCTTTCCCGATAAGTTGGAAATCCGGTGGAAGCTTTCTGGATGCGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATg                                                      >  2:108807/1‑150 (MQ=255)
                                                                                                  tCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGa                                                     <  1:475511/150‑1 (MQ=255)
                                                                                                   cAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGTATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTCACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGac                                                    <  1:298201/150‑1 (MQ=255)
                                                                                                   cAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGac                                                    >  2:1721310/1‑150 (MQ=255)
                                                                                                    aGCTTTCCCGATAAGTTTGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGaca                                                   <  2:111144/150‑1 (MQ=255)
                                                                                                      cTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACg                                                 <  2:1618480/150‑1 (MQ=255)
                                                                                                                 aGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCGCCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTc                                      <  1:1573680/150‑1 (MQ=255)
                                                                                                                  gtaggAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCCAATGCGAGGCGCGCGTATGACACGCAACCCCCGTcc                                     >  1:1433494/4‑149 (MQ=255)
                                                                                                                   ttGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCATATGACACGCAAACACCGTCGc                                    >  1:240539/1‑150 (MQ=255)
                                                                                                                     ggAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCa                                  >  1:916243/1‑150 (MQ=255)
                                                                                                                          tCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGctc                             >  2:639595/1‑150 (MQ=255)
                                                                                                                            cGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCtt                           >  1:1523831/1‑150 (MQ=255)
                                                                                                                               tGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCt                        <  2:540024/150‑1 (MQ=255)
                                                                                                                                  aaGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTATCCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtgtg                     <  1:1276511/150‑1 (MQ=255)
                                                                                                                                    gCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGcc                   <  1:1410242/150‑1 (MQ=255)
                                                                                                                                      tttCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCg                 >  1:869242/1‑150 (MQ=255)
                                                                                                                                       ttCTGGATGAGCAGCCTGCTCATCATATTTATGAAGTAATTCAGATCCCCTCTTCACCGTATTAACCGATGAGAAAACGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGt                >  2:154099/1‑150 (MQ=255)
                                                                                                                                       aatatgATGAGCAGGCTGCTCATCATATTTATGCAGTAATTGAGATCCACTCTTCACCGTATTAACCGATGAGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCGGt                >  2:589850/6‑150 (MQ=255)
                                                                                                                                           ggATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGGATGACACGCAAACCCCGTCGCCCCGCGCTTTCTGTGCCCGTgcgc            >  1:1081062/1‑150 (MQ=255)
                                                                                                                                            gATGAGCAGGCTGCTCATCATATTTTTGGAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGCGCGAATGCGAGGCGCGCGTATGACACGCAACCCCCGTCGCCCCGCTCTTTCTGTGCCCGTgcgcc           >  2:825478/1‑149 (MQ=255)
                                                                                                                                                   agcCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCCCCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTc    <  2:1508902/150‑1 (MQ=255)
                                                                                                                                                   agcCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTc    <  1:1420481/150‑1 (MQ=255)
                                                                                                                                                   agcCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTc    <  1:1589263/150‑1 (MQ=255)
                                                                                                                                                   agcCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTc    <  2:379351/150‑1 (MQ=255)
                                                                                                                                                   agcCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTc    >  1:560970/1‑150 (MQ=255)
                                                                                                                                                    gcCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCg   <  1:1543779/150‑1 (MQ=255)
                                                                                                                                                     ccTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCgg  <  2:1673688/150‑1 (MQ=255)
                                                                                                                                                     ccTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCgg  <  2:927002/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGG  >  NC_000913/3354388‑3354686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: