Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,385,836 G→A 5.7% intergenic (+35/+21) yhcN → / ← yhcO cadmium and peroxide resistance protein, stress‑induced/putative barnase inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,385,8360GA 5.7% 502.8 / 9.7 157intergenic (+35/+21)yhcN/yhcOcadmium and peroxide resistance protein, stress‑induced/putative barnase inhibitor
Reads supporting (aligned to +/- strand):  ref base G (79/67);  new base A (2/7);  total (81/76)
Fisher's exact test for biased strand distribution p-value = 8.73e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

GGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTA  >  NC_000913/3385687‑3385984
                                                                                                                                                     |                                                                                                                                                     
     tGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCgg                                                                                                                                                 >  1:1288800/1‑150 (MQ=255)
      gCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCggg                                                                                                                                                >  1:1417374/1‑150 (MQ=255)
      gCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCggg                                                                                                                                                <  1:1568990/150‑1 (MQ=255)
      gCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCggg                                                                                                                                                >  2:1590198/1‑150 (MQ=255)
        gTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCggggg                                                                                                                                              <  1:1690072/150‑1 (MQ=255)
         tGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGc                                                                                                                                             <  1:1249375/150‑1 (MQ=255)
         tGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGAACCGGGGGc                                                                                                                                             <  1:126466/150‑1 (MQ=255)
         ggAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCGCGTCGCGGGGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCCTCGTCTTGTCCGCCGATATTGCCCCCGAACCGGGGGc                                                                                                                                             <  1:1216155/149‑1 (MQ=255)
             aTGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGAACCGGGGGcaata                                                                                                                                         <  2:956468/150‑5 (MQ=255)
              tGCTGAACAAAAAAGCGGAAGAGAAAGGCTCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGAACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGAACCGGGGGcaatat                                                                                                                                        <  1:1522933/150‑6 (MQ=255)
              tGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGAACCGGGGGcaatat                                                                                                                                        <  1:1340152/150‑6 (MQ=255)
               gCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATTAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCtttttt                                                                                                                                       <  2:697355/150‑1 (MQ=255)
                cTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCttttttt                                                                                                                                      >  1:239952/1‑150 (MQ=255)
                 tGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGCCACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCtttttttt                                                                                                                                     >  2:956606/1‑150 (MQ=255)
                   aaCAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTgc                                                                                                                                   <  2:935633/150‑1 (MQ=255)
                    aCAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTgcg                                                                                                                                  <  2:457405/150‑1 (MQ=255)
                    aCAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTgcg                                                                                                                                  <  1:36579/150‑1 (MQ=255)
                     cAAAAAAGCGGAAGAGAAAGTCGCACCGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACCACTGGCACGCTACGGCTGATCAGGACAAATTAACCCTCATCGGCTTGGCCGACGATATTGCCGGCGGAGCGGGGGCTTTTTTTTgcgt                                                                                                                                 >  1:106995/1‑149 (MQ=255)
                     cAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTgcgc                                                                                                                                 >  1:64418/1‑150 (MQ=255)
                       aaaaaGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTa                                                                                                                               >  1:1189212/1‑150 (MQ=255)
                           aGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGa                                                                                                                           <  1:1000574/150‑1 (MQ=255)
                                 agagAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCgggg                                                                                                                                               >  1:1527420/1‑124 (MQ=255)
                                 agagAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCgggg                                                                                                                                               <  2:1527420/124‑1 (MQ=255)
                                  gagaAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACAt                                                                                                                    >  1:166174/1‑150 (MQ=255)
                                    gaAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTa                                                                                                                  <  1:1443436/150‑1 (MQ=255)
                                    gaAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTa                                                                                                                  >  2:777488/1‑150 (MQ=255)
                                       aGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTaaaa                                                                                                               <  1:954631/150‑1 (MQ=255)
                                       aGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACAATCATCGTCTTGTCCGACGATATTGCCCCCCGTTCGGGCGCTAAATTTTCCGCTAATGCCGAACATTaaaa                                                                                                               >  2:1243361/1‑150 (MQ=255)
                                           gcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCa                                                                                                           <  2:777009/150‑1 (MQ=255)
                                           gcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCa                                                                                                           <  2:1479705/150‑1 (MQ=255)
                                           gcAACGGCCTACAAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCa                                                                                                           <  2:347764/150‑1 (MQ=255)
                                               cGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATg                                                                                                       <  1:336003/150‑1 (MQ=255)
                                                    tACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCtt                                                                                                  >  2:1158690/1‑150 (MQ=255)
                                                     aCCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTTCGCTAATGACGAACATTAAAACGCAAATGCCCTTc                                                                                                 >  2:111271/1‑150 (MQ=255)
                                                     aCCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTc                                                                                                 >  1:485232/1‑150 (MQ=255)
                                                     aCCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTc                                                                                                 >  1:1005106/1‑150 (MQ=255)
                                                      ccAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                                                <  2:1339832/150‑1 (MQ=255)
                                                       cAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGTGCTAATGACGAACATTAAAACGCAAATGCCCTTCCa                                                                                               <  1:457284/150‑1 (MQ=255)
                                                       cAGATAACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCa                                                                                               <  1:1424858/150‑1 (MQ=255)
                                                        aGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAg                                                                                              <  1:1460417/150‑1 (MQ=255)
                                                         gATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACTATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGc                                                                                             <  1:214446/150‑1 (MQ=255)
                                                            tACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGctct                                                                                          <  2:1636146/150‑1 (MQ=255)
                                                            tACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGctct                                                                                          <  2:1417374/150‑1 (MQ=255)
                                                            tACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGctct                                                                                          <  1:833089/150‑1 (MQ=255)
                                                             aCTGAAGCTCCTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCtt                                                                                         >  2:547758/1‑150 (MQ=255)
                                                                gAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTccg                                                                                      >  1:780091/1‑149 (MQ=255)
                                                                gAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGACCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCct                                                                                      >  1:959322/1‑150 (MQ=255)
                                                                 aaGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCct                                                                                      <  2:505480/150‑1 (MQ=255)
                                                                 aaGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCctc                                                                                     >  2:759960/1‑150 (MQ=255)
                                                                  aGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCctct                                                                                    <  2:1562220/150‑1 (MQ=255)
                                                                   gCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                                                   <  2:984697/150‑1 (MQ=255)
                                                                   gCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                                                   >  1:1373787/1‑150 (MQ=255)
                                                                      cGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCt                                                                                <  2:1615496/150‑1 (MQ=255)
                                                                      cGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCt                                                                                >  1:250601/1‑150 (MQ=255)
                                                                      cGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCt                                                                                <  1:501732/150‑1 (MQ=255)
                                                                      cGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGGTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCt                                                                                <  1:1677391/150‑1 (MQ=255)
                                                                        tAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtca                                                                              <  1:1590198/150‑1 (MQ=255)
                                                                          gCGGGGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAGAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatc                                                                            <  1:1729564/150‑1 (MQ=255)
                                                                               gACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACa                                                                       <  1:1660427/150‑1 (MQ=255)
                                                                                 cacCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGc                                                                     <  2:1446739/150‑1 (MQ=255)
                                                                                   ccTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCaa                                                                   >  1:1032013/1‑150 (MQ=255)
                                                                                    cTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAat                                                                  <  1:582526/150‑1 (MQ=255)
                                                                                    cTGACACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAat                                                                  >  2:1492275/1‑150 (MQ=255)
                                                                                     tGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAata                                                                 >  2:1126528/1‑150 (MQ=255)
                                                                                        cACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTa                                                              <  2:375048/150‑1 (MQ=255)
                                                                                             tACGGCTGAACTGTACTAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTGTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGATCTTCCTCTGCCTCATCAAACAGCAATATTAActcc                                                         >  2:1655342/1‑147 (MQ=255)
                                                                                                ggCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCa                                                      <  2:664011/150‑1 (MQ=255)
                                                                                                      aCTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCACCCGGTTCGGGGGCTGATTTTTGCGCTAATGCCGAACATTAAAACGCAACTGCACTTCCAGCTCTTCATCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACgc                                                >  2:1319636/1‑149 (MQ=255)
                                                                                                            cAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAcgc                                          >  1:354150/1‑150 (MQ=255)
                                                                                                            cAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAcgc                                          >  1:933339/1‑150 (MQ=255)
                                                                                                                tAAACCCTCATCGTCTTGTCCGACGATAATGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAACACGAAAATGCCCTTCCAGCTCTTGCTCTGCCTCATCATAAAGCAATATTAAAGAGCCAAAACGGCGCcgaattt                                      >  1:106046/1‑145 (MQ=255)
                                                                                                                 aaaCCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGGCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGtttt                                     <  1:899433/150‑1 (MQ=255)
                                                                                                                 aaaCCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGtttt                                     >  2:445391/1‑150 (MQ=255)
                                                                                                                   aCCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtct                                   <  2:581334/150‑1 (MQ=255)
                                                                                                                    cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtctc                                  >  2:871778/1‑150 (MQ=255)
                                                                                                                    cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATAAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtctc                                  <  2:1096701/150‑1 (MQ=255)
                                                                                                                      cTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtctctc                                >  1:189363/1‑150 (MQ=255)
                                                                                                                        cATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTccc                              >  1:593555/1‑150 (MQ=255)
                                                                                                                         aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCa                             >  1:807886/1‑150 (MQ=255)
                                                                                                                            gTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGAt                          <  2:1032013/150‑1 (MQ=255)
                                                                                                                            gTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTGTTCTCTCCCAGATg                         >  2:597151/1‑150 (MQ=255)
                                                                                                                               ttGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGaa                       <  2:663465/150‑1 (MQ=255)
                                                                                                                                tGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACa                     <  1:1631622/150‑1 (MQ=255)
                                                                                                                                 gTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACa                     >  1:1314442/1‑150 (MQ=255)
                                                                                                                                  tCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACaa                    >  2:838896/1‑150 (MQ=255)
                                                                                                                                  tCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACaa                    >  2:491928/1‑150 (MQ=255)
                                                                                                                                  tCCGACGATATTGCCCCCGGTTAGGGGGCTATTATTTGCGCTAATGACTAACATTAAAACTCAAATGCACTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCAAAAACGGCGACTCTGATTCTCTCCCAGATGAACaa                    >  1:102556/1‑150 (MQ=255)
                                                                                                                                    cgacgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAAt                  >  1:692379/1‑150 (MQ=255)
                                                                                                                                     gacgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGATAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGGTTTCTCTCCCAGCTGAACAAAtt                 >  2:1478645/1‑150 (MQ=255)
                                                                                                                                      acgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTc                <  1:995802/150‑1 (MQ=255)
                                                                                                                                      acgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTc                <  2:74149/150‑1 (MQ=255)
                                                                                                                                       aaaaaaagCCCCCGAACCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCa               >  2:233790/8‑150 (MQ=255)
                                                                                                                                       aaaaaaagCCCCCGAACCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCa               >  2:831375/8‑150 (MQ=255)
                                                                                                                                         atatTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAt             <  2:423485/150‑1 (MQ=255)
                                                                                                                                           atTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCATACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAtct           <  2:64418/150‑1 (MQ=255)
                                                                                                                                             tGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCa         >  2:1386351/1‑150 (MQ=255)
                                                                                                                                              gCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTGCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAACCGGCGACGCGATTTCTCTCCCAGATGAACAAATTCAATCTCaa        >  1:1340198/1‑150 (MQ=255)
                                                                                                                                                 cccGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTATCCCAGATGAACAAATACAATATCAAGTg     >  2:563920/1‑150 (MQ=255)
                                                                                                                                                  ccGGTTCTGGGGCTTTTTTTTGCGCTAATGACCAACATTAAACCGCAAATGCCCGACCCGCACTTCCTCTGCCTCATCAAACATAATTAATAACGCGCCAAAACGGCGACGCGGTTTCTCTCCCAGATGAACAACTTCAATCTCAAGTgg    <  2:910937/150‑1 (MQ=255)
                                                                                                                                                    ggTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTa  >  1:945976/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTA  >  NC_000913/3385687‑3385984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: