Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,397,965 G→T 6.7% V138V (GTC→GTA) ‡ yhdE ← dTTP/UTP pyrophosphatase; m(5)UTP/m(5)CTP/pseudo‑UTP pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,397,9650GT 6.7% 375.2 / 10.5 119V138V (GTC→GTA) ‡yhdEdTTP/UTP pyrophosphatase; m(5)UTP/m(5)CTP/pseudo‑UTP pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base G (50/61);  new base T (3/5);  total (53/66)
Fisher's exact test for biased strand distribution p-value = 7.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

Only 100 of 120 total aligned reads displayed.

AGTGCGTTAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGC  >  NC_000913/3397816‑3398111
                                                                                                                                                     |                                                                                                                                                  
aGTGCGTTTAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTTTCTTCCTGACAAAACAGCCACCCAGCCCCTGTATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCg                                                                                                                                                    <  2:606876/150‑1 (MQ=255)
  tGCGTTAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGAc                                                                                                                                                  <  2:351015/150‑1 (MQ=255)
     gTTAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATa                                                                                                                                               >  1:606124/1‑150 (MQ=255)
         aaaTTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGccc                                                                                                                                           <  1:1355910/150‑1 (MQ=255)
            ttACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCcgcg                                                                                                                                        <  2:772421/150‑1 (MQ=255)
            ttACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCcgcg                                                                                                                                        <  2:1246396/150‑1 (MQ=255)
              aCTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGAt                                                                                                                                      <  2:1424619/150‑1 (MQ=255)
              aCTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGAt                                                                                                                                      <  2:1556956/150‑1 (MQ=255)
                ttAATAATTCATACCTTACACCCACCTGAAAGGCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACTCAACAGCCTCCCACCCCCGGAATACCGTCTGCACCTGCTTTATCTAACGGTGCATCGCTGGCTATGTCGCCAGCGATga                                                                                                                                    <  1:243653/150‑2 (MQ=255)
                 taataaTTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCGGGCTATGTCGCCAGCGATgaa                                                                                                                                   <  1:203640/150‑3 (MQ=255)
                  aataatTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCt                                                                                                                                  <  1:1506785/150‑1 (MQ=255)
                  aataatTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCGGGCTATGTCGCCAGCGATgaac                                                                                                                                  <  1:512681/150‑4 (MQ=255)
                  aataatTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCGGGCTATGTCGCCAGCGATgaac                                                                                                                                  <  2:232861/150‑4 (MQ=255)
                   ataatTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCtt                                                                                                                                 >  1:1388524/1‑150 (MQ=255)
                    taatTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCGGGCTATGTCGCCAGCGATgaaccg                                                                                                                                <  1:46813/150‑6 (MQ=255)
                     aatTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCg                                                                                                                               >  2:939179/1‑150 (MQ=255)
                       ttCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTc                                                                                                                             <  2:606124/150‑1 (MQ=255)
                        tCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCt                                                                                                                            <  1:1327567/150‑1 (MQ=255)
                         cATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTg                                                                                                                           >  2:535843/1‑150 (MQ=255)
                          aTACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACATCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGt                                                                                                                          <  1:1522445/150‑1 (MQ=255)
                            aCGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTa                                                                                                                        <  1:939179/150‑1 (MQ=255)
                              gTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGCCCTAGCCCGCGATGTCTTCGTCTGATAAc                                                                                                                      >  2:1322069/1‑150 (MQ=255)
                                  cAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCCCCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGGCTGGTTACggtg                                                                                                                  >  2:754054/1‑147 (MQ=255)
                                  cAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCGCTGAATACCGTATGCGCCTGCTTTATCTAACGGTTCATCGCTGGCGACGTAGCCCGCGGTGGCTGCGTCTGTGAACGTTc                                                                                                                  >  2:843308/1‑150 (MQ=255)
                                  cAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTc                                                                                                                  <  1:205864/150‑1 (MQ=255)
                                    aCCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTg                                                                                                                <  2:1417050/150‑1 (MQ=255)
                                     ccAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTTTGCCCCTGCTTTATCTAACGGTTCATCGCTGGCGACCTAGCCCGGGATGTATTCGTCTGTTTACGTTCTGa                                                                                                               >  2:1135181/1‑150 (MQ=255)
                                     ccAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGa                                                                                                               >  1:1710608/1‑150 (MQ=255)
                                        gCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAg                                                                                                            <  2:357301/150‑1 (MQ=255)
                                        gCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAg                                                                                                            <  2:233869/150‑1 (MQ=255)
                                         cGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCGCCCAGCAGCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTTACGTTCTGAAAGt                                                                                                           >  1:1294070/1‑150 (MQ=255)
                                         cGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGt                                                                                                           >  1:752937/1‑150 (MQ=255)
                                         cGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGt                                                                                                           <  2:908313/150‑1 (MQ=255)
                                           gTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTca                                                                                                         <  1:601642/150‑1 (MQ=255)
                                             aaGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTcaca                                                                                                       >  1:351575/1‑150 (MQ=255)
                                             aaGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTATGTTAACGTTCTGAAAGTcaca                                                                                                       >  1:1578577/1‑150 (MQ=255)
                                               gCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATc                                                                                                     <  1:1110319/150‑1 (MQ=255)
                                                ccGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCg                                                                                                    >  2:1386496/1‑150 (MQ=255)
                                                 cGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCgg                                                                                                   <  2:489455/150‑1 (MQ=255)
                                                   tcTACGGCTTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTg                                                                                                 <  1:569475/149‑1 (MQ=255)
                                                   aCTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTg                                                                                                 <  1:444685/150‑1 (MQ=255)
                                                   aCTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTg                                                                                                 <  2:1585148/150‑1 (MQ=255)
                                                      aCGGCGTGATAGCTGCCATTTATCTTACTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGTAGACATAGCCCTCGATGTCATCGTCTGTTAACGTTCTGAAAGTCACATCGGTGacc                                                                                              >  1:1576340/1‑150 (MQ=255)
                                                       cGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGTCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGacca                                                                                             >  1:1418958/1‑150 (MQ=255)
                                                        ggCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGaccac                                                                                            <  1:1715333/150‑1 (MQ=255)
                                                            tGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATCCCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAgg                                                                                        <  1:1526793/150‑1 (MQ=255)
                                                                  cTGCCATTTATCTTCCTGACAAACCAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGCCCACCAGGCATTCg                                                                                  >  2:208129/1‑150 (MQ=255)
                                                                  cTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCg                                                                                  <  2:459748/150‑1 (MQ=255)
                                                                     ccATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGGCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCgaaa                                                                               <  1:1140843/150‑3 (MQ=255)
                                                                      cATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCTGTGACCACCAGGCAATCGAGaa                                                                              <  1:283269/150‑1 (MQ=255)
                                                                       aTTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAAt                                                                             <  2:432840/150‑1 (MQ=255)
                                                                        tttATCTTCCTGACAAAACAGCAACCCAGCCACTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAAtg                                                                            <  2:1602610/150‑1 (MQ=255)
                                                                          tATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCCCCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAAtgtg                                                                          >  2:1497231/1‑150 (MQ=255)
                                                                          tATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAAtgtg                                                                          <  1:278089/150‑1 (MQ=255)
                                                                           aTCTTCCTGCCAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGCCATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACCTCGGTGACCCCCAGGCAATCGAGAATGTgc                                                                         >  1:729432/1‑150 (MQ=255)
                                                                                  tGACAAAACAGCCACCCAGCCCCTGAATACAGTATGAACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGACTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCAACACGCAAACGAGAATGTGCTGGCTGt                                                                  >  1:541666/1‑150 (MQ=255)
                                                                                   gACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTc                                                                 >  2:28873/1‑150 (MQ=255)
                                                                                   gACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTc                                                                 >  2:1255199/1‑150 (MQ=255)
                                                                                    aCAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCg                                                                >  1:934451/1‑150 (MQ=255)
                                                                                     cAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGTCATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCTTTAGTGAATTTGCTGGCTGTCgg                                                               >  2:1008122/1‑150 (MQ=255)
                                                                                     cAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCgg                                                               >  2:1669814/1‑150 (MQ=255)
                                                                                             ccACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGcc                                                       <  1:1496055/150‑1 (MQ=255)
                                                                                             ccACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGcc                                                       >  1:351167/1‑150 (MQ=255)
                                                                                                cccAGCCCCTGAGTACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCAct                                                    <  1:598721/150‑1 (MQ=255)
                                                                                                    gCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCActgctg                                                <  2:1108587/150‑1 (MQ=255)
                                                                                                      cccTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGtc                                              >  1:350497/1‑150 (MQ=255)
                                                                                                            aTACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCAcc                                        <  2:165027/150‑1 (MQ=255)
                                                                                                              aCCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTg                                      <  2:577361/150‑1 (MQ=255)
                                                                                                              aCCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGACTGTCGGCCAACGCCACTGCTGTCATCACCTg                                      <  1:1637436/150‑1 (MQ=255)
                                                                                                               ccGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGa                                     >  2:46297/1‑150 (MQ=255)
                                                                                                               ccGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGa                                     >  1:1159460/1‑150 (MQ=255)
                                                                                                                 gTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATg                                   <  2:932810/150‑1 (MQ=255)
                                                                                                                 gTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATg                                   <  1:938588/150‑1 (MQ=255)
                                                                                                                 gTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATg                                   <  2:924873/150‑1 (MQ=255)
                                                                                                                 gTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATg                                   >  1:391606/1‑150 (MQ=255)
                                                                                                                  tATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATgg                                  >  1:929019/1‑150 (MQ=255)
                                                                                                                   aTGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATggg                                 <  2:466354/150‑1 (MQ=255)
                                                                                                                    tGCACCTGCGTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGt                                <  1:1266389/150‑1 (MQ=255)
                                                                                                                     gCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTc                               <  2:707116/150‑1 (MQ=255)
                                                                                                                     gCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTc                               <  2:752937/150‑1 (MQ=255)
                                                                                                                     gCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTc                               <  1:332429/150‑1 (MQ=255)
                                                                                                                      cACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCGTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGCCCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCt                              >  1:70683/1‑150 (MQ=255)
                                                                                                                        ccTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACAACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGa                            >  1:1136760/1‑150 (MQ=255)
                                                                                                                         cTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGat                           <  1:1386496/150‑2 (MQ=255)
                                                                                                                          tGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAATGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCAAACGCCACTGCTGTCATCACCTGATGGGTCTGAcc                          <  1:1263292/150‑1 (MQ=255)
                                                                                                                           gCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGAccc                         <  1:513371/150‑1 (MQ=255)
                                                                                                                           gCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGAccc                         >  1:1483567/1‑150 (MQ=255)
                                                                                                                           gCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGAccc                         >  1:785939/1‑150 (MQ=255)
                                                                                                                              ttATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGAt                      >  1:1181055/1‑150 (MQ=255)
                                                                                                                                  cTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAAtt                  >  2:167182/1‑150 (MQ=255)
                                                                                                                                   tAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAAttt                 >  2:903935/1‑150 (MQ=255)
                                                                                                                                    aaCGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTg                <  2:2031/150‑1 (MQ=255)
                                                                                                                                     cgaagacATCGCGGGCTATGTCGCCAGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTgc               >  1:664413/7‑150 (MQ=255)
                                                                                                                                       ggTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTACCGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTgcgc             <  1:1306355/150‑1 (MQ=255)
                                                                                                                                         ttCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGaaa           >  2:1489226/1‑150 (MQ=255)
                                                                                                                                           cATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCCCCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCCCCTGATGGGTCTGACCCGATAATTTGCGCAACa         >  2:1084840/1‑150 (MQ=255)
                                                                                                                                           cATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCATTCGAGAATGTGCTGGCTGTCGGCCAACGACACTGCTGTCATCACCTGATGGGTCTGCCCCGATAATTTGCGCAACa         >  1:1040026/1‑150 (MQ=255)
                                                                                                                                           cATCGCGGGCTATGTCGCCAGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACa         >  2:1517038/1‑150 (MQ=255)
                                                                                                                                           cATCGCGGGCTATGTCGCCAGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACa         >  2:754121/1‑150 (MQ=255)
                                                                                                                                                  ggCGACATAGCCAGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCACCATCTgcgc  >  1:528034/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AGTGCGTTAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGC  >  NC_000913/3397816‑3398111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: