Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,397,970 A→C 6.8% Y137D (TAT→GAT) ‡ yhdE ← dTTP/UTP pyrophosphatase; m(5)UTP/m(5)CTP/pseudo‑UTP pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,397,9700AC 6.8% 339.9 / 12.8 118Y137D (TAT→GAT) ‡yhdEdTTP/UTP pyrophosphatase; m(5)UTP/m(5)CTP/pseudo‑UTP pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base A (50/60);  new base C (3/5);  total (53/65)
Fisher's exact test for biased strand distribution p-value = 7.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.03e-01

Only 100 of 119 total aligned reads displayed.

GTTAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGC  >  NC_000913/3397821‑3398118
                                                                                                                                                     |                                                                                                                                                    
gTTAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATa                                                                                                                                                      >  1:606124/1‑150 (MQ=255)
   aaaaTTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACACAACAGCCCCCCCGCCCCTGAATACCGTATGCCCCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGcc                                                                                                                                                   >  2:1028234/1‑150 (MQ=255)
    aaaTTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGccc                                                                                                                                                  <  1:1355910/150‑1 (MQ=255)
       ttACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCcgcg                                                                                                                                               <  2:772421/150‑1 (MQ=255)
       ttACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCcgcg                                                                                                                                               <  2:1246396/150‑1 (MQ=255)
         aCTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGAGTACGGCGTGATTGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGAt                                                                                                                                             <  1:1573318/150‑1 (MQ=255)
           ttAATAATTCATACCTTACACCCACCTGAAAGGCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACTCAACAGCCTCCCACCCCCGGAATACCGTCTGCACCTGCTTTATCTAACGGTGCATCGCTGGCTATGTCGCCAGCGATga                                                                                                                                           <  1:243653/150‑2 (MQ=255)
            taataaTTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTc                                                                                                                                          >  2:1615300/1‑150 (MQ=255)
            taataaTTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCGGGCTATGTCGCCAGCGATgaa                                                                                                                                          <  1:203640/150‑3 (MQ=255)
             aataatTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCt                                                                                                                                         <  1:1506785/150‑1 (MQ=255)
             aataatTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCGGGCTATGTCGCCAGCGATgaac                                                                                                                                         <  1:512681/150‑4 (MQ=255)
             aataatTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCGGGCTATGTCGCCAGCGATgaac                                                                                                                                         <  2:232861/150‑4 (MQ=255)
              ataatTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCtt                                                                                                                                        >  1:1388524/1‑150 (MQ=255)
                aatTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCg                                                                                                                                      >  2:939179/1‑150 (MQ=255)
                  ttCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTc                                                                                                                                    <  2:606124/150‑1 (MQ=255)
                    cATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTg                                                                                                                                  >  2:535843/1‑150 (MQ=255)
                    cATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCCGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTg                                                                                                                                  <  2:214033/150‑1 (MQ=255)
                     aTACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACATCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGt                                                                                                                                 <  1:1522445/150‑1 (MQ=255)
                       aCGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTa                                                                                                                               <  1:939179/150‑1 (MQ=255)
                         gTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGCCCTAGCCCGCGATGTCTTCGTCTGATAAc                                                                                                                             >  2:1322069/1‑150 (MQ=255)
                             cAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCCCCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGGCTGGTTACggtg                                                                                                                         >  2:754054/1‑147 (MQ=255)
                             cAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCGCTGAATACCGTATGCGCCTGCTTTATCTAACGGTTCATCGCTGGCGACGTAGCCCGCGGTGGCTGCGTCTGTGAACGTTc                                                                                                                         >  2:843308/1‑150 (MQ=255)
                             cAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACGTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTc                                                                                                                         >  2:357484/1‑150 (MQ=255)
                               aCCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTg                                                                                                                       <  2:1417050/150‑1 (MQ=255)
                                ccAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGa                                                                                                                      >  2:1263292/1‑150 (MQ=255)
                                ccAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGa                                                                                                                      >  1:1710608/1‑150 (MQ=255)
                                   gCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAg                                                                                                                   <  2:233869/150‑1 (MQ=255)
                                   gCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAg                                                                                                                   <  2:357301/150‑1 (MQ=255)
                                    cGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCGCCCAGCAGCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTTACGTTCTGAAAGt                                                                                                                  >  1:1294070/1‑150 (MQ=255)
                                    cGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGt                                                                                                                  >  1:752937/1‑150 (MQ=255)
                                    cGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGt                                                                                                                  <  2:908313/150‑1 (MQ=255)
                                      gTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTca                                                                                                                <  1:601642/150‑1 (MQ=255)
                                        aaGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTcaca                                                                                                              >  1:351575/1‑150 (MQ=255)
                                          gCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATc                                                                                                            <  1:1110319/150‑1 (MQ=255)
                                           ccGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCg                                                                                                           >  2:1386496/1‑150 (MQ=255)
                                              tcTACGGCTTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTg                                                                                                        <  1:569475/149‑1 (MQ=255)
                                              aCTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTg                                                                                                        <  1:444685/150‑1 (MQ=255)
                                              aCTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTg                                                                                                        <  2:1585148/150‑1 (MQ=255)
                                                 aCGGCGTGATAGCTGCCATTTATCTTACTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGTAGACATAGCCCTCGATGTCATCGTCTGTTAACGTTCTGAAAGTCACATCGGTGacc                                                                                                     >  1:1576340/1‑150 (MQ=255)
                                                  cGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGTCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGacca                                                                                                    >  1:1418958/1‑150 (MQ=255)
                                                   ggCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGaccac                                                                                                   <  1:1715333/150‑1 (MQ=255)
                                                       tGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATCCCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAgg                                                                                               <  1:1526793/150‑1 (MQ=255)
                                                             cTGCCATTTATCTTCCTGACAAACCAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGCCCACCAGGCATTCg                                                                                         >  2:208129/1‑150 (MQ=255)
                                                             cTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCg                                                                                         <  2:459748/150‑1 (MQ=255)
                                                                ccATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGGCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCgaaa                                                                                      <  1:1140843/150‑3 (MQ=255)
                                                                 cATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCTGTGACCACCAGGCAATCGAGaa                                                                                     <  1:283269/150‑1 (MQ=255)
                                                                  aTTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAAt                                                                                    <  2:432840/150‑1 (MQ=255)
                                                                   tttATCTTCCTGACAAAACAGCAACCCAGCCACTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAAtg                                                                                   <  2:1602610/150‑1 (MQ=255)
                                                                     tATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCCCCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAAtgtg                                                                                 >  2:1497231/1‑150 (MQ=255)
                                                                     tATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAAtgtg                                                                                 >  2:513371/1‑150 (MQ=255)
                                                                     tATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAAtgtg                                                                                 <  1:278089/150‑1 (MQ=255)
                                                                             tGACAAAACAGCCACCCAGCCCCTGAATACAGTATGAACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGACTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCAACACGCAAACGAGAATGTGCTGGCTGt                                                                         >  1:541666/1‑150 (MQ=255)
                                                                              gACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTc                                                                        >  2:1255199/1‑150 (MQ=255)
                                                                               aCAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCg                                                                       >  1:934451/1‑150 (MQ=255)
                                                                                cAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGTCATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCTTTAGTGAATTTGCTGGCTGTCgg                                                                      >  2:1008122/1‑150 (MQ=255)
                                                                                cAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCgg                                                                      >  2:1669814/1‑150 (MQ=255)
                                                                                       gCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGc                                                               <  1:856022/150‑1 (MQ=255)
                                                                                        ccACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGcc                                                              >  1:351167/1‑150 (MQ=255)
                                                                                        ccACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGcc                                                              <  1:1496055/150‑1 (MQ=255)
                                                                                          aCCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCAc                                                            <  1:535843/150‑1 (MQ=255)
                                                                                               gCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCActgctg                                                       <  2:1108587/150‑1 (MQ=255)
                                                                                                      aaTACCGTATGCACCGGCTTTCTCTAACGGTTCAGCGCTGGCGACATAGCCCGCGATGTCTTCGGCTGTTAACGTGCTGAAAGTCACATCTGTGACCACCAGTCAATCTAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCAc                                                <  1:292730/150‑1 (MQ=255)
                                                                                                       aTACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCAcc                                               <  2:165027/150‑1 (MQ=255)
                                                                                                         aCCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTg                                             >  1:192481/1‑150 (MQ=255)
                                                                                                         aCCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTg                                             <  2:577361/150‑1 (MQ=255)
                                                                                                         aCCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGACTGTCGGCCAACGCCACTGCTGTCATCACCTg                                             <  1:1637436/150‑1 (MQ=255)
                                                                                                          ccGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGa                                            >  1:1159460/1‑150 (MQ=255)
                                                                                                          ccGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGa                                            >  2:46297/1‑150 (MQ=255)
                                                                                                            gTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATg                                          <  2:924873/150‑1 (MQ=255)
                                                                                                            gTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATg                                          <  1:415899/150‑1 (MQ=255)
                                                                                                            gTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATg                                          <  2:932810/150‑1 (MQ=255)
                                                                                                            gTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATg                                          >  1:391606/1‑150 (MQ=255)
                                                                                                             tATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATgg                                         >  1:929019/1‑150 (MQ=255)
                                                                                                              aTGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATggg                                        <  2:466354/150‑1 (MQ=255)
                                                                                                               tGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGt                                       <  1:1322069/150‑1 (MQ=255)
                                                                                                               tGCACCTGCGTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGt                                       <  1:1266389/150‑1 (MQ=255)
                                                                                                                gCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTc                                      <  1:332429/150‑1 (MQ=255)
                                                                                                                gCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTc                                      <  2:707116/150‑1 (MQ=255)
                                                                                                                gCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTc                                      <  2:752937/150‑1 (MQ=255)
                                                                                                                 cACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCGTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGCCCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCt                                     >  1:70683/1‑150 (MQ=255)
                                                                                                                   ccTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACAACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGa                                   >  1:1136760/1‑150 (MQ=255)
                                                                                                                    cTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGat                                  <  1:1386496/150‑2 (MQ=255)
                                                                                                                     tGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAATGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCAAACGCCACTGCTGTCATCACCTGATGGGTCTGAcc                                 <  1:1263292/150‑1 (MQ=255)
                                                                                                                      gCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGAccc                                >  1:785939/1‑150 (MQ=255)
                                                                                                                      gCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGAccc                                <  1:513371/150‑1 (MQ=255)
                                                                                                                         ttATCTAACGGTTCGTCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGaa                             >  1:641862/1‑149 (MQ=255)
                                                                                                                              tAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAAttt                        >  2:903935/1‑150 (MQ=255)
                                                                                                                               aaCGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTg                       <  2:2031/150‑1 (MQ=255)
                                                                                                                               aaCGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTg                       >  2:1490761/1‑150 (MQ=255)
                                                                                                                                cgaagacATCGCGGGCTATGTCGCCAGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTgc                      >  1:664413/7‑150 (MQ=255)
                                                                                                                                aCGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTgc                      <  1:1161927/150‑1 (MQ=255)
                                                                                                                                  ggTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTACCGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTgcgc                    <  1:1306355/150‑1 (MQ=255)
                                                                                                                                  ggTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGGCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTgcgc                    <  1:1438527/150‑1 (MQ=255)
                                                                                                                                    ttCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGaaa                  >  2:1489226/1‑150 (MQ=255)
                                                                                                                                      cATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCCCCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCCCCTGATGGGTCTGACCCGATAATTTGCGCAACa                >  2:1084840/1‑150 (MQ=255)
                                                                                                                                      cATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCATTCGAGAATGTGCTGGCTGTCGGCCAACGACACTGCTGTCATCACCTGATGGGTCTGCCCCGATAATTTGCGCAACa                >  1:1040026/1‑150 (MQ=255)
                                                                                                                                      cATCGCGGGCTATGTCGCCAGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACa                >  2:1517038/1‑150 (MQ=255)
                                                                                                                                      cATCGCGGGCTATGTCGCCAGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACa                >  2:754121/1‑150 (MQ=255)
                                                                                                                                             ggCGACATAGCCAGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCACCATCTgcgc         >  1:528034/1‑150 (MQ=255)
                                                                                                                                                    tAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGc  >  2:1478538/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTTAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGC  >  NC_000913/3397821‑3398118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: