Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,407,293 T→A 10.7% intergenic (+27/‑82) accC → / → yhdT acetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,407,2930TA10.7% 276.3 / 21.8 112intergenic (+27/‑82)accC/yhdTacetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (43/55);  new base A (4/8);  total (48/64)
Fisher's exact test for biased strand distribution p-value = 5.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.54e-02

Only 100 of 116 total aligned reads displayed.

ATCATCGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTA  >  NC_000913/3407144‑3407439
                                                                                                                                                     |                                                                                                                                                   
atcatcGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAt                                                                                                                                                     <  1:1101710/150‑1 (MQ=255)
  catcGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAttt                                                                                                                                                   >  1:432892/1‑150 (MQ=255)
   atcGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCCTGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGACTTCAGGAAAAATAAGACTGCTAAAGCAACAAAAGGCAGGAtttt                                                                                                                                                  >  1:334574/1‑150 (MQ=255)
     cGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTAAGGAAAAATAAGACTGCTAAAGAGTTAAAAGGCCGGAgtttac                                                                                                                                                >  2:1461304/1‑148 (MQ=255)
       aCGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCgg                                                                                                                                              <  1:803651/150‑1 (MQ=255)
       aCGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCgg                                                                                                                                              <  1:762880/150‑1 (MQ=255)
          gTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCt                                                                                                                                          <  1:265361/150‑1 (MQ=255)
           tATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCtt                                                                                                                                         <  2:1120276/150‑1 (MQ=255)
           tATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCtt                                                                                                                                         <  2:296487/150‑1 (MQ=255)
           tATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCtt                                                                                                                                         <  2:461010/150‑1 (MQ=255)
            aTCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttt                                                                                                                                        <  1:911334/150‑1 (MQ=255)
             tCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCtttt                                                                                                                                       <  1:1028121/150‑1 (MQ=255)
              cAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCttttt                                                                                                                                      >  1:956686/1‑150 (MQ=255)
              cAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCttttt                                                                                                                                      >  2:1492051/1‑150 (MQ=255)
              cAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCttttt                                                                                                                                      >  2:1277372/1‑150 (MQ=255)
              cAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCttttt                                                                                                                                      >  1:1248975/1‑150 (MQ=255)
              cAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCttttt                                                                                                                                      >  2:640522/1‑150 (MQ=255)
              cAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCAAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTACGGCCttttt                                                                                                                                      <  1:238842/150‑1 (MQ=255)
               aaaaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCtttttt                                                                                                                                     <  2:40119/150‑1 (MQ=255)
               aaaaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCtttttt                                                                                                                                     >  2:1354304/1‑150 (MQ=255)
                aaaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttttgac                                                                                                                                    <  1:1659364/150‑4 (MQ=255)
                 aaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTttat                                                                                                                                   <  2:599201/150‑1 (MQ=255)
                 aaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTttat                                                                                                                                   <  2:940492/150‑1 (MQ=255)
                 aaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTttat                                                                                                                                   <  1:497936/150‑1 (MQ=255)
                   ccAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTttatta                                                                                                                                 >  2:1132532/1‑150 (MQ=255)
                    cAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTAc                                                                                                                                >  1:1588641/1‑150 (MQ=255)
                    cAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTAc                                                                                                                                <  2:1032112/150‑1 (MQ=255)
                     aaCGTTGATCTGCAGATCAGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACt                                                                                                                               <  2:855946/150‑1 (MQ=255)
                       cGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGATATAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTgg                                                                                                                             <  2:1013713/150‑1 (MQ=255)
                       cGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTgg                                                                                                                             <  1:447115/150‑1 (MQ=255)
                        gTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTTGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGGTAAATCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTggg                                                                                                                            <  1:1222228/150‑1 (MQ=255)
                        gTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTggg                                                                                                                            <  1:532530/150‑1 (MQ=255)
                          tGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGa                                                                                                                          <  2:2556/150‑1 (MQ=255)
                             tCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCg                                                                                                                       <  1:315350/150‑1 (MQ=255)
                             tCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGACAGGATTTTCCGGCCATTTTTATTACTGGGGATCg                                                                                                                       >  1:132955/1‑150 (MQ=255)
                              cTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGa                                                                                                                      >  2:1430503/1‑150 (MQ=255)
                              cTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGa                                                                                                                      <  2:1316938/150‑1 (MQ=255)
                               tGCAGATCCGCATCATTAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGAc                                                                                                                     <  2:1201121/150‑1 (MQ=255)
                                  aGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttttg                                                                                                                                      <  2:1323857/130‑2 (MQ=255)
                                  aGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttttg                                                                                                                                      >  1:1323857/1‑129 (MQ=255)
                                   gATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAAcc                                                                                                                 <  2:945675/150‑1 (MQ=255)
                                   gATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACAATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCATTTTTATTACTGGGGATCGCCAAcc                                                                                                                 >  2:730035/1‑150 (MQ=255)
                                           tcatGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAg                                                                                                         <  2:885494/150‑1 (MQ=255)
                                           tcatGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAg                                                                                                         <  2:1337883/150‑1 (MQ=255)
                                            catGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGGCTGCTAAAGCGTCAAAAAGCCGGATTTTCCGGCCTTTTTTTATTACTGGGGAACGACAAccccataaag                                                                                                         >  2:1492016/1‑144 (MQ=255)
                                             atgaatgaCGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGt                                                                                                       <  1:1098862/150‑1 (MQ=255)
                                              tgaatgaCGAGCACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTGCAGGAAAAATAAGACTGCTACAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTa                                                                                                      >  2:196885/1‑150 (MQ=255)
                                               gaatgaCGAGAACATCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCTTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTAc                                                                                                     <  1:1007684/150‑1 (MQ=255)
                                                          aCTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCAAAAGGTACAATCCCCGCtt                                                                                          >  1:1531590/1‑150 (MQ=255)
                                                               cAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttcttc                                                                                     <  1:467229/150‑1 (MQ=255)
                                                                aGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCa                                                                                    <  1:1697341/150‑1 (MQ=255)
                                                                aGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCa                                                                                    <  1:1703821/150‑1 (MQ=255)
                                                                 gCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTTCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCAc                                                                                   <  1:1144888/150‑1 (MQ=255)
                                                                  cATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCAcc                                                                                  <  2:1620556/150‑1 (MQ=255)
                                                                    tggtggCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGTATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCa                                                                                <  2:1034672/150‑1 (MQ=255)
                                                                     ggtggCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCAt                                                                               >  1:1166621/1‑150 (MQ=255)
                                                                       tggCACTAACATCCACTATCTGGAGAAATAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCa                                                                             <  2:59458/150‑1 (MQ=255)
                                                                          cACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAggg                                                                          <  1:1001665/150‑1 (MQ=255)
                                                                          cACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAggg                                                                          >  2:1475114/1‑150 (MQ=255)
                                                                          cACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAggg                                                                          <  2:810304/150‑1 (MQ=255)
                                                                           aCTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGa                                                                         >  2:387837/1‑150 (MQ=255)
                                                                              aaCATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGAACAATCCCCGCTTTCTTCACCCAGCAGGGACaa                                                                      >  2:153364/1‑150 (MQ=255)
                                                                                  tCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACaaaaaa                                                                  <  1:387837/150‑1 (MQ=255)
                                                                                     aCTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCTGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATgg                                                               >  2:1247899/1‑150 (MQ=255)
                                                                                      cTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGa                                                              <  1:640522/150‑1 (MQ=255)
                                                                                        aTCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGaca                                                            <  2:531150/150‑1 (MQ=255)
                                                                                               gaAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGttt                                                     <  2:1390471/150‑1 (MQ=255)
                                                                                               gaAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGttt                                                     <  1:907972/150‑1 (MQ=255)
                                                                                                  aaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGt                                                  >  2:590810/1‑150 (MQ=255)
                                                                                                     acgcGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCAGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCa                                               <  1:1295631/147‑1 (MQ=255)
                                                                                                     aCTCTGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCa                                               <  1:1302544/150‑1 (MQ=255)
                                                                                                       tCGGTCTACAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCCGATTTTCAGGACTTTTTTATTACTGGGGATCGACAACAACCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTAGTTCAgg                                             >  1:631130/1‑150 (MQ=255)
                                                                                                          gTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGccc                                          >  2:596540/1‑150 (MQ=255)
                                                                                                            cTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCAt                                        >  1:1435424/1‑150 (MQ=255)
                                                                                                             ttCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATa                                       <  2:1729736/150‑1 (MQ=255)
                                                                                                               cAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATaaa                                     >  2:787839/1‑150 (MQ=255)
                                                                                                                 ggAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAaga                                   <  1:634209/150‑1 (MQ=255)
                                                                                                                 ggAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAaga                                   >  1:640309/1‑150 (MQ=255)
                                                                                                                  gAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCCCCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAagag                                  >  2:1558843/1‑150 (MQ=255)
                                                                                                                           gACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctgg                         >  1:1421258/1‑150 (MQ=255)
                                                                                                                                  aaaGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACACCCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctgggcgctgg                  <  2:241413/150‑1 (MQ=255)
                                                                                                                                   aaGCGGCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAACGTACAATCCCCGCTTTCTTCCCCCATCAGGGACAAAAAATGGCCACTCGTTTTGTTCAGGCCCATAAAGAGGCGcgctgggcgcgggg                 <  2:746438/150‑1 (MQ=255)
                                                                                                                                    aGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTgggg                <  2:1533329/150‑1 (MQ=255)
                                                                                                                                      cGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCt              >  1:901799/1‑150 (MQ=255)
                                                                                                                                      cGTCAAAAGGACGGATTTTCCGGCATTTTTTATTACTGGGGATCGACAACCCCCATAATGTACAATCCCCGCTTTCTTCACACATCAGGGACAAAAAATGGACCCTCGTTTTGTTCCTGCCCATAAAGAGGCGCGCTGGGCGCTGGGCCt              >  2:78776/1‑150 (MQ=255)
                                                                                                                                        tCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGa            >  2:209509/1‑150 (MQ=255)
                                                                                                                                         aaaaaGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGAc           <  2:1314921/149‑1 (MQ=255)
                                                                                                                                          aaaaGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGAcc          <  1:724081/150‑1 (MQ=255)
                                                                                                                                           aaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGCCACCCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCCGGGCAAAAAAATGGACAATCGTTTTTTTCAGGCCCATAAAGAGGCGCGCTGGGCGGTGGGGCTGcccc         >  2:355928/1‑150 (MQ=255)
                                                                                                                                           aaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGAccc         >  2:928383/1‑150 (MQ=255)
                                                                                                                                            aaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCt        >  2:1416534/1‑150 (MQ=255)
                                                                                                                                              ggacGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCttt      <  2:432892/147‑1 (MQ=255)
                                                                                                                                              ggCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCttt      <  1:1430503/150‑1 (MQ=255)
                                                                                                                                              ggCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGCGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCttt      <  1:369982/150‑1 (MQ=255)
                                                                                                                                               gCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATTCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTAGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGATGCCCCattt     >  2:876832/1‑150 (MQ=255)
                                                                                                                                               gCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCtttt     <  2:400885/150‑1 (MQ=255)
                                                                                                                                                ccGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGCCCCTTTTg    >  1:675606/1‑150 (MQ=255)
                                                                                                                                                 cGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGt   >  2:1161432/1‑150 (MQ=255)
                                                                                                                                                 cGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGt   >  2:1412960/1‑150 (MQ=255)
                                                                                                                                                  ggATTTTCCGGCCTTTTTTATTTCTGGGGATCGACAACCCCCATAAGGGACAATCCCCGCTTTCTTCCCCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATATAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTa  <  2:956686/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ATCATCGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTA  >  NC_000913/3407144‑3407439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: