Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,407,294 T→A 10.7% intergenic (+28/‑81) accC → / → yhdT acetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,407,2940TA10.7% 283.9 / 21.7 112intergenic (+28/‑81)accC/yhdTacetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (44/56);  new base A (4/8);  total (48/64)
Fisher's exact test for biased strand distribution p-value = 5.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.91e-01

Only 100 of 115 total aligned reads displayed.

CATCGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTA  >  NC_000913/3407146‑3407439
                                                                                                                                                    |                                                                                                                                                  
 atcGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCCTGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGACTTCAGGAAAAATAAGACTGCTAAAGCAACAAAAGGCAGGAtttt                                                                                                                                                  >  1:334574/1‑150 (MQ=255)
   cGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTAAGGAAAAATAAGACTGCTAAAGAGTTAAAAGGCCGGAgtttac                                                                                                                                                >  2:1461304/1‑148 (MQ=255)
     aCGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCgg                                                                                                                                              <  1:803651/150‑1 (MQ=255)
     aCGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCgg                                                                                                                                              <  1:762880/150‑1 (MQ=255)
        gTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCt                                                                                                                                          <  1:265361/150‑1 (MQ=255)
         tATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCtt                                                                                                                                         >  2:1471393/1‑150 (MQ=255)
         tATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCtt                                                                                                                                         <  2:296487/150‑1 (MQ=255)
         tATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCtt                                                                                                                                         <  2:461010/150‑1 (MQ=255)
          aTCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttt                                                                                                                                        <  1:911334/150‑1 (MQ=255)
          aTCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttt                                                                                                                                        <  2:474562/150‑1 (MQ=255)
           tCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCtttt                                                                                                                                       <  1:1028121/150‑1 (MQ=255)
            cAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCttttt                                                                                                                                      >  2:1277372/1‑150 (MQ=255)
            cAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCttttt                                                                                                                                      >  1:1248975/1‑150 (MQ=255)
            cAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCttttt                                                                                                                                      >  1:956686/1‑150 (MQ=255)
            cAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCttttt                                                                                                                                      >  2:1492051/1‑150 (MQ=255)
            cAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCAAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTACGGCCttttt                                                                                                                                      <  1:238842/150‑1 (MQ=255)
            cAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTACCATCATGGTGGAACTAACATCCACTATCAGGAGAAAAAACACGGACTTCAGGAAAAATAACACAGCTAAAGCGTCAAAAGGCCGGATTGTCCCGCCtttat                                                                                                                                      >  1:1330283/1‑148 (MQ=255)
            cAAAAAAAACGTTGATCTTAAGATCCGCATCATGAATGACGAGAACTTACAGCATGTTGGCACTAACATAAACGATAAGGAGAAAAAACTAGTTCTTCAGGAAAAATAAGACTGCTAACGCGTCAAAAGGCAGGATTTTCCGGCCtgttt                                                                                                                                      >  2:1195727/1‑150 (MQ=255)
             aaaaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCtttttt                                                                                                                                     <  2:40119/150‑1 (MQ=255)
             aaaaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCtttttt                                                                                                                                     >  2:1354304/1‑150 (MQ=255)
              aaaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttttgac                                                                                                                                    <  1:1659364/150‑4 (MQ=255)
               aaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTttat                                                                                                                                   <  2:940492/150‑1 (MQ=255)
               aaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTttat                                                                                                                                   <  2:599201/150‑1 (MQ=255)
               aaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTttat                                                                                                                                   <  1:497936/150‑1 (MQ=255)
                 ccAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTttatta                                                                                                                                 >  2:1132532/1‑150 (MQ=255)
                  cAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTAc                                                                                                                                >  1:1588641/1‑150 (MQ=255)
                  cAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTAc                                                                                                                                <  2:1032112/150‑1 (MQ=255)
                   aaCGTTGATCTGCAGATCCGCATCATGATTGACGAGAACTTACAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACt                                                                                                                               <  2:1341405/150‑1 (MQ=255)
                   aaCGTTGATCTGCAGATCAGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACt                                                                                                                               <  2:855946/150‑1 (MQ=255)
                     cGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGATATAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTgg                                                                                                                             <  2:1013713/150‑1 (MQ=255)
                     cGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTgg                                                                                                                             <  1:447115/150‑1 (MQ=255)
                      gTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTggg                                                                                                                            <  1:532530/150‑1 (MQ=255)
                      gTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTggg                                                                                                                            <  1:1212130/150‑1 (MQ=255)
                         gATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTGTTCAGGATAAATAAGACTGCTAAGGCGTCAAAGGGCCGGAGTTTCGGGCGGTTTTTATTACTgggggg                                                                                                                         >  2:1581987/1‑148 (MQ=255)
                           tCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCg                                                                                                                       <  1:315350/150‑1 (MQ=255)
                           tCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGACAGGATTTTCCGGCCATTTTTATTACTGGGGATCg                                                                                                                       >  1:132955/1‑150 (MQ=255)
                            cTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGa                                                                                                                      >  2:1430503/1‑150 (MQ=255)
                             tGCAGATCCGCATCATTAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGAc                                                                                                                     <  2:1201121/150‑1 (MQ=255)
                                aGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttttg                                                                                                                                      >  1:1323857/1‑129 (MQ=255)
                                aGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttttg                                                                                                                                      <  2:1323857/130‑2 (MQ=255)
                                 gATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAAcc                                                                                                                 <  2:945675/150‑1 (MQ=255)
                                 gATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACAATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCATTTTTATTACTGGGGATCGCCAAcc                                                                                                                 >  2:730035/1‑150 (MQ=255)
                                    ccGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAAccccc                                                                                                              >  2:1001665/1‑150 (MQ=255)
                                         tcatGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAg                                                                                                         <  2:1337883/150‑1 (MQ=255)
                                         tcatGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAg                                                                                                         <  2:885494/150‑1 (MQ=255)
                                          catGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGGCTGCTAAAGCGTCAAAAAGCCGGATTTTCCGGCCTTTTTTTATTACTGGGGAACGACAAccccataaag                                                                                                         >  2:1492016/1‑144 (MQ=255)
                                           atgaatgaCGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGt                                                                                                       <  1:1098862/150‑1 (MQ=255)
                                            tgaatgaCGAGCACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTGCAGGAAAAATAAGACTGCTACAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTa                                                                                                      >  2:196885/1‑150 (MQ=255)
                                             gaatgaCGAGAACATCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCTTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTAc                                                                                                     <  1:1007684/150‑1 (MQ=255)
                                                      gaACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGc                                                                                            >  2:724081/1‑150 (MQ=255)
                                                        aCTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCAAAAGGTACAATCCCCGCtt                                                                                          >  1:1531590/1‑150 (MQ=255)
                                                             cAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttcttc                                                                                     <  1:467229/150‑1 (MQ=255)
                                                              aGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCa                                                                                    <  1:1697341/150‑1 (MQ=255)
                                                               gCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTTCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCAc                                                                                   <  1:1144888/150‑1 (MQ=255)
                                                                cATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCAcc                                                                                  <  2:1620556/150‑1 (MQ=255)
                                                                 aTGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGCTCGACAACCACCAGAAGGTACACTCACCGCTTTCGTCAcac                                                                                 >  1:610187/1‑148 (MQ=255)
                                                                  tggtggCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGTATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCa                                                                                <  2:1034672/150‑1 (MQ=255)
                                                                   ggtggCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCAt                                                                               >  1:1166621/1‑150 (MQ=255)
                                                                    gtgtCACTTACATCCACTATCTTGAGAAAAAACTCGGTCTTCAGGAAAATTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTATTCACCCATc                                                                              <  1:1675893/150‑1 (MQ=255)
                                                                     tggCACTAACATCCACTATCTGGAGAAATAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCa                                                                             <  2:59458/150‑1 (MQ=255)
                                                                        cACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAggg                                                                          <  1:1001665/150‑1 (MQ=255)
                                                                        cACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAggg                                                                          <  2:810304/150‑1 (MQ=255)
                                                                        cACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAggg                                                                          >  2:1475114/1‑150 (MQ=255)
                                                                         aCTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGa                                                                         >  2:387837/1‑150 (MQ=255)
                                                                                tCCACTCTCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCTAACGGCCGGAGTTACCGGCCTTTTTTATTACTGGGGAGCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACaaaaaa                                                                  <  2:256057/150‑1 (MQ=255)
                                                                                tCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACaaaaaa                                                                  <  1:387837/150‑1 (MQ=255)
                                                                                   aCTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCTGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATgg                                                               >  2:1247899/1‑150 (MQ=255)
                                                                                    cTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGa                                                              <  1:640522/150‑1 (MQ=255)
                                                                                      aTCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGaca                                                            <  2:531150/150‑1 (MQ=255)
                                                                                             gaAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGttt                                                     <  1:907972/150‑1 (MQ=255)
                                                                                             gaAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGttt                                                     <  2:1390471/150‑1 (MQ=255)
                                                                                                aaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGt                                                  >  2:590810/1‑150 (MQ=255)
                                                                                                   acgcGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCAGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCa                                               <  1:1295631/147‑1 (MQ=255)
                                                                                                   aCTCTGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCa                                               <  1:1302544/150‑1 (MQ=255)
                                                                                                     tCGGTCTACAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCCGATTTTCAGGACTTTTTTATTACTGGGGATCGACAACAACCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTAGTTCAgg                                             >  1:631130/1‑150 (MQ=255)
                                                                                                        gTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGccc                                          >  2:596540/1‑150 (MQ=255)
                                                                                                          cTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCAt                                        >  1:1435424/1‑150 (MQ=255)
                                                                                                           ttCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATa                                       <  2:1729736/150‑1 (MQ=255)
                                                                                                             cAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATaaa                                     >  2:787839/1‑150 (MQ=255)
                                                                                                               ggAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAaga                                   <  1:634209/150‑1 (MQ=255)
                                                                                                               ggAAAAATAAGACTGCTAAAGCGTAAAAAGGCAGGATTTTCCGGCCGTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATCGCCACTCGTTTAGTTCAGACCCATAAaga                                   >  1:752091/1‑150 (MQ=255)
                                                                                                                gAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCCCCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAagag                                  >  2:1558843/1‑150 (MQ=255)
                                                                                                                         gACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctgg                         >  1:1421258/1‑150 (MQ=255)
                                                                                                                                aaaGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACACCCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctgggcgctgg                  <  2:241413/150‑1 (MQ=255)
                                                                                                                                 aaGCGGCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAACGTACAATCCCCGCTTTCTTCCCCCATCAGGGACAAAAAATGGCCACTCGTTTTGTTCAGGCCCATAAAGAGGCGcgctgggcgcgggg                 <  2:746438/150‑1 (MQ=255)
                                                                                                                                  aGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTgggg                <  2:1533329/150‑1 (MQ=255)
                                                                                                                                    cGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCACCGCTTTCTTCACCAATCAGGGACAAAAAATGGAAACTCGTTTTGTTCAGTCACATAAAGAGGCGCGCTGGGCGCTGGGCCt              >  1:337914/1‑150 (MQ=255)
                                                                                                                                    cGTCAAAAGGACGGATTTTCCGGCATTTTTTATTACTGGGGATCGACAACCCCCATAATGTACAATCCCCGCTTTCTTCACACATCAGGGACAAAAAATGGACCCTCGTTTTGTTCCTGCCCATAAAGAGGCGCGCTGGGCGCTGGGCCt              >  2:78776/1‑150 (MQ=255)
                                                                                                                                      tCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGa            >  2:209509/1‑150 (MQ=255)
                                                                                                                                       aaaaaGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGAc           <  2:1314921/149‑1 (MQ=255)
                                                                                                                                        aaaaGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGAcc          <  1:724081/150‑1 (MQ=255)
                                                                                                                                         aaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGCCACCCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCCGGGCAAAAAAATGGACAATCGTTTTTTTCAGGCCCATAAAGAGGCGCGCTGGGCGGTGGGGCTGcccc         >  2:355928/1‑150 (MQ=255)
                                                                                                                                         aaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGAccc         >  2:928383/1‑150 (MQ=255)
                                                                                                                                            ggCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGCGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCttt      <  1:369982/150‑1 (MQ=255)
                                                                                                                                             gCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATTCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTAGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGATGCCCCattt     >  2:876832/1‑150 (MQ=255)
                                                                                                                                             gCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCtttt     <  2:400885/150‑1 (MQ=255)
                                                                                                                                              ccGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGCCCCTTTTg    >  1:675606/1‑150 (MQ=255)
                                                                                                                                               cGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGt   >  2:1412960/1‑150 (MQ=255)
                                                                                                                                               cGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGt   >  2:1161432/1‑150 (MQ=255)
                                                                                                                                                ggATTTTCCGGCCTTTTTTATTTCTGGGGATCGACAACCCCCATAAGGGACAATCCCCGCTTTCTTCCCCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATATAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTa  <  2:956686/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CATCGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTA  >  NC_000913/3407146‑3407439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: