BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	422,537	T→G	6.1%	L7R (CTG→CGG)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	422,537	0	T	G	6.1%	325.7 / 10.7	115	L7R (CTG→CGG)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand): ref base T (47/60); new base G (3/4); total (51/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.09e-01

Only 100 of 116 total aligned reads displayed.

AAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGC  >  NC_000913/422390‑422686

aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTGCGCGCCCACATATCGTGGAGGGGCGGAGGGGTCCTGCATTAGGCGAAGGCAAGGTGGTCAGGTTTTCGTGACAGGGGAATTATTATGTTTTTTGCGTGGCAGCTGc                                                                                                                                                     >  2:293936/1‑150 (MQ=255)

   tCCTGATCCGGCTATTCCGAAAGTTATCCGCCCACGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCgg                                                                                                                                                  <  2:250652/150‑1 (MQ=255)

    ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGt                                                                                                                                                 <  1:1548608/150‑1 (MQ=255)

         tCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGcccc                                                                                                                                            <  2:723125/150‑1 (MQ=255)

          ccGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCCGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCGGCAGGTGccatg                                                                                                                                           <  2:460934/150‑4 (MQ=255)

           cGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCa                                                                                                                                          <  1:1170861/150‑1 (MQ=255)

            ggCTATTCCGAAAGTAATCCGCCCCCGTCCTCCTCACCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCGGCAGGTGccatgca                                                                                                                                         <  2:72200/150‑6 (MQ=255)

              cTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCGGCAGGTGccatgcatt                                                                                                                                       <  1:646148/150‑8 (MQ=255)

                  tCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTa                                                                                                                                   <  1:605424/150‑1 (MQ=255)

                  tCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCAAAAAAGCTACAGAAGAAGAGAGATCAGGCATTAGGGAATGTCATTGTGATGAGATTTTAAAAACATGTGAATTATGATTTTAAATTAATGTAACATAACGGTGGCCCCaaatgtat                                                                                                                                   >  2:1618266/1‑143 (MQ=255)

                  tCCGAAAGTTATCCGCCCCCGGCCTCCGCCCGCAAAAATACTTCAGATGATGAATGCTACTGCATTAGGCGATGGCAAGGGGTAAAGATTTTGATATCAGGGGAGTAATGATGTTAAATGCATGGAACCATACCGTGCCCCCAGATGTTa                                                                                                                                   >  2:1624629/1‑150 (MQ=255)

                   ccGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTaa                                                                                                                                  <  1:1350014/150‑1 (MQ=255)

                     gAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAc                                                                                                                                <  2:1521980/150‑1 (MQ=255)

                     gAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAc                                                                                                                                <  1:890595/150‑1 (MQ=255)

                     gAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAc                                                                                                                                <  2:633392/150‑1 (MQ=255)

                         tttATCCGCCCCCGTCCTCCTCCCCAAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGGGCCCCCATTTGTTAAAcaaag                                                                                                                            <  2:193513/149‑1 (MQ=255)

                            aTCCGCCGCCGTCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGGGATGGCAAGGTGAGCAGATTTTCAACAAAAGGGAATTATGATGTTAAATGCAATGCACCTGCCGGTGCCCCCAGGAGTTAAAcaaagcaa                                                                                                                         >  2:1128583/1‑150 (MQ=255)

                              ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaag                                                                                                                       <  2:584012/150‑1 (MQ=255)

                                 cccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCCGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATc                                                                                                                    <  2:1481258/150‑1 (MQ=255)

                                 cccccGTCCTCCTCCCCCAAAAATACTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATc                                                                                                                    >  2:970093/1‑150 (MQ=255)

                                   cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCACTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCaa                                                                                                                  >  1:833478/1‑150 (MQ=255)

                                        cctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATAACAGTGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAACCAAAGCAAAGATCAACTGCt                                                                                                             <  1:283242/150‑1 (MQ=255)

                                         ctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACA‑GGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCa                                                                                                           <  2:124641/150‑1 (MQ=255)

                                           cctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCAt                                                                                                          <  2:1021785/150‑1 (MQ=255)

                                              cccccAAATATCCTTCAGCTGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCCCCTGCCGGTGCCCCCATTTGTTAAACAACGCAAAGCTCTACTGCTCATTac                                                                                                       >  2:251312/1‑150 (MQ=255)

                                              cccccAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTac                                                                                                       <  1:1218772/150‑1 (MQ=255)

                                              cccccAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTac                                                                                                       <  1:1297789/150‑1 (MQ=255)

                                               ccccAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTaca                                                                                                      >  1:812429/1‑150 (MQ=255)

                                               ccccAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTaca                                                                                                      >  2:1206333/1‑150 (MQ=255)

                                                cccAAATATCCTTCAGATGATGAGTGATCCTTCATTAGGCTATGGCAAGGTTATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTacac                                                                                                     <  2:1127558/150‑1 (MQ=255)

                                                cccAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATATTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTacac                                                                                                     >  2:1094743/1‑150 (MQ=255)

                                                cccAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGATATGGCAAGGTGATCATATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTacac                                                                                                     <  1:609537/150‑1 (MQ=255)

                                                 ccAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACt                                                                                                    <  2:1526313/150‑1 (MQ=255)

                                                   aaaTATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACtgt                                                                                                  <  2:1586318/150‑1 (MQ=255)

                                                    aaTATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTTATCAGATTTTCATCACAGGTTAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACtgtg                                                                                                 <  1:1607010/150‑1 (MQ=255)

                                                    aaTATCCTTCAGATGATGAGTGATCCTGCATTAGGCTAGGGCAAGGTGATCAGATGTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCACCATATGTTAAACAAAGCAAAGATCCACTGCGAATTACACtggg                                                                                                 >  2:819809/1‑148 (MQ=255)

                                                     atatCCTTCAGATGATGAGTGATGCTGCATTTGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTgg                                                                                                <  2:677606/150‑1 (MQ=255)

                                                     atatCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTgg                                                                                                <  2:894319/150‑1 (MQ=255)

                                                     atatCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTCATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTgg                                                                                                <  2:903258/150‑1 (MQ=255)

                                                          cTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGAc                                                                                           <  1:773188/150‑1 (MQ=255)

                                                          cTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGAc                                                                                           >  2:374205/1‑150 (MQ=255)

                                                             cAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACggg                                                                                        <  2:1705701/150‑1 (MQ=255)

                                                             cAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACggg                                                                                        <  2:84227/150‑1 (MQ=255)

                                                             cAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACggg                                                                                        >  1:1265645/1‑150 (MQ=255)

                                                             cAGATGATGACTGATCCTGCATTAGGATATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACggg                                                                                        <  1:277142/150‑1 (MQ=255)

                                                              aGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGc                                                                                       >  2:374929/1‑150 (MQ=255)

                                                               gatgatGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCACCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCg                                                                                      >  1:234599/1‑150 (MQ=255)

                                                               gatgagGACGGATCCAGCAGTAGCCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACACAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCg                                                                                      <  2:1130966/150‑1 (MQ=255)

                                                                 tgatgaGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGaa                                                                                    >  2:62945/1‑150 (MQ=255)

                                                                  gatgaGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAg                                                                                   <  1:970093/150‑1 (MQ=255)

                                                                  gatgaGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAg                                                                                   <  1:362561/150‑1 (MQ=255)

                                                                    tgaGTGATCCTGCACTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGAc                                                                                 <  1:1398223/150‑1 (MQ=255)

                                                                          aTCCTGCATTAGGCTATGGCAAGGTGATCAGATTTCCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCg                                                                           >  2:201602/1‑150 (MQ=255)

                                                                              tgccatAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAgc                                                                       <  1:142851/145‑1 (MQ=255)

                                                                               gCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAgcg                                                                      <  1:1544512/150‑1 (MQ=255)

                                                                                 aTTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGAACCTGCCGGTGACCCCATTTGTTAAACAAAGAAAAGATCAACTGCTCATTACACTGTGGCTGAAGGGCGATGACCCACCGAAGCGCa                                                                    >  1:1558449/1‑150 (MQ=255)

                                                                                     ggCTATGGCAAGGTGATCAGATATTCATCACAGGGTAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGTCTGACGGGCGAATACCCACCGCAGCGCATTAt                                                                <  1:639330/150‑1 (MQ=255)

                                                                                       cTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTAtgc                                                              >  2:1659185/1‑150 (MQ=255)

                                                                                         aTGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGCCCCACCGCAGCGCATTAtgctg                                                            >  2:676025/1‑150 (MQ=255)

                                                                                          tGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGCCGGGCGAAGACCCACCGCAGCGCATTAtgctgc                                                           >  2:250635/1‑150 (MQ=255)

                                                                                              aatttGATCAGATTTTCATCACAGGGTAATTATGATTTTAAATGAATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACTGGCGAAGACCCACCGCAGCGCATTATGCTGCGTAc                                                       <  1:1469278/146‑1 (MQ=255)

                                                                                                ggTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAg                                                     <  1:995669/150‑1 (MQ=255)

                                                                                                ggTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAg                                                     >  1:1659943/1‑150 (MQ=255)

                                                                                                ggTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACAGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAg                                                     <  1:1632282/150‑1 (MQ=255)

                                                                                                   tatatgATTTTCATCA‑ACGGGTAATATGATGTTAAATGCATGTCACCTGCCGGTGCCCACATTTGTTAAACAAAGCAAATATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAaca                                                 <  2:376113/145‑1 (MQ=255)

                                                                                                   gATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAac                                                  >  1:871028/1‑150 (MQ=255)

                                                                                                    aTCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAaca                                                 <  1:177809/150‑1 (MQ=255)

                                                                                                     tCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAacac                                                <  1:166365/150‑1 (MQ=255)

                                                                                                        gATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTAt                                                                >  1:1663432/1‑131 (MQ=255)

                                                                                                        gATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTAt                                                                <  2:1663432/131‑1 (MQ=255)

                                                                                                        gATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTTCAGAACACGAt                                             >  1:800988/1‑150 (MQ=255)

                                                                                                          atttCATCACAGGCGAATAATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAATCAAAGCAAAGATGAACTGCTCATTACACTGTGGCTGACTGTCGATGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATaa                                           <  1:157553/149‑1 (MQ=255)

                                                                                                             tcatcaCAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAGGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACga                                        <  1:452744/150‑1 (MQ=255)

                                                                                                               atcaCAGGGTAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAGCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACgaag                                      <  2:440140/150‑1 (MQ=255)

                                                                                                               atcaCAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACgaag                                      >  2:937642/1‑150 (MQ=255)

                                                                                                                   caGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAAt                                  >  1:1606781/1‑150 (MQ=255)

                                                                                                                   caGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAAt                                  <  1:996139/150‑1 (MQ=255)

                                                                                                                   caGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAAt                                  >  2:896240/1‑150 (MQ=255)

                                                                                                                     ggggAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGt                                >  1:1550508/1‑150 (MQ=255)

                                                                                                                     ggggAATTATGATGTTAAATACATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCCCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGt                                >  1:1434185/1‑150 (MQ=255)

                                                                                                                      gggAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTc                               >  1:1447513/1‑150 (MQ=255)

                                                                                                                        gAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGTCGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAg                             <  1:1258340/150‑1 (MQ=255)

                                                                                                                           ttATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTAc                          >  2:1391820/1‑150 (MQ=255)

                                                                                                                            tATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTAcc                         >  2:1002983/1‑150 (MQ=255)

                                                                                                                             atgatgTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCg                        >  2:1613076/1‑150 (MQ=255)

                                                                                                                              tgatgTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATAGACTGCTCATTACCCTGTGGCTGACGGGCGAAGCCCCACCGCAGCGAATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGa                       >  1:404576/1‑150 (MQ=255)

                                                                                                                                    tAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATa                 <  2:614745/150‑1 (MQ=255)

                                                                                                                                    tAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATa                 <  1:1455348/150‑1 (MQ=255)

                                                                                                                                     aaaTGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATaa                >  2:1005872/1‑150 (MQ=255)

                                                                                                                                     aaaTGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATaa                >  2:1282722/1‑150 (MQ=255)

                                                                                                                                       aatgggggCACCGGCAGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCATACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAagc              >  1:691967/7‑150 (MQ=255)

                                                                                                                                       aTGCACGGCACCTGCCGGTGCCACCATTTGTTAAACAAAGCAACGATCAACTGCTCATTACACTGTTGCTTACGGGCGAAGACCCACCGCAGCGCACTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAagc              <  2:708742/150‑1 (MQ=255)

                                                                                                                                          ggaggCACCGGCAGCTGCCCCCATTTCATAAACAAAGCAAAGATCAACTGCGCATTACACTGTGGCTGACGGTCCAAGTCCCAACCCAGCGCATTAAGATGCGTAAATAACACGAAACCGAATAAATGTACTTACAAATGCAAAagcagc           >  2:1386525/4‑150 (MQ=255)

                                                                                                                                          cATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAagcagc           >  2:423569/1‑150 (MQ=255)

                                                                                                                                           ggggCACCGGCAGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAgcg          >  1:1512644/3‑150 (MQ=255)

                                                                                                                                           aTGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAgcg          >  2:806148/1‑150 (MQ=255)

                                                                                                                                             ggCACCTGCCGGTGCCCGCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCa        >  1:1124248/1‑150 (MQ=255)

                                                                                                                                                 ccTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCa    >  2:79242/1‑150 (MQ=255)

                                                                                                                                                 ccTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGAGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCa    <  1:293936/150‑1 (MQ=255)

                                                                                                                                                   tGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGc  >  2:1650759/1‑150 (MQ=255)

AAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGC  >  NC_000913/422390‑422686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`