BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,423,622	C→T	8.3%	noncoding (34/76 nt)	thrV ←	tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,423,622	0	C	T	8.3%	320.6 / 12.7	108	noncoding (34/76 nt)	thrV	tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base C (55/44); new base T (3/6); total (58/50)
Fisher's exact test for biased strand distribution p-value = 2.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01

Only 100 of 108 total aligned reads displayed.

CGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGAC  >  NC_000913/3423474‑3423764

cgcTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCa                                                                                                                                               <  1:1716897/150‑1 (MQ=255)

cgcTACGGCGTTTCACTTCTGAGTTCGGCAGGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCTAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCa                                                                                                                                               <  1:122377/150‑1 (MQ=255)

   tACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGGCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAgg                                                                                                                                            <  2:263295/150‑1 (MQ=255)

    atggCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAATAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAggg                                                                                                                                           <  1:57560/148‑1 (MQ=255)

    aCGGCGTCTCACTTCTGAGTTCGGCATTGGGCCATGCGGGACCACCGCGCTACTGGCGCCAGACAAATTCTATGCTACTCTTCCTAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAggg                                                                                                                                           <  1:310204/150‑1 (MQ=38)

        cGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTgag                                                                                                                                       <  2:639631/150‑3 (MQ=255)

         gTTTCACTTCTGAGTGCGGCATGGGGGCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTGGTAAGGGTgagg                                                                                                                                      <  2:737473/150‑4 (MQ=37)

         gTTGCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTGGTAAGGGTgagg                                                                                                                                      <  2:1409215/150‑4 (MQ=37)

          tttCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTGGTAAGGGTgaggt                                                                                                                                     <  1:444765/150‑5 (MQ=255)

          tttCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTGGTAAGGGTgaggt                                                                                                                                     <  1:1009285/150‑5 (MQ=255)

           ttCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCGCACCCTTGGTAAGGGTgaggtc                                                                                                                                    <  1:1384617/150‑6 (MQ=37)

           ttCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGAAACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGctc                                                                                                                                    <  1:616902/150‑1 (MQ=255)

            tCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTTTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCCTGGTAAGGGTgaggtcc                                                                                                                                   <  1:11853/150‑7 (MQ=25)

             cACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTa                                                                                                                                  <  1:1272025/150‑1 (MQ=255)

              aCTTCTGAGTTCGGCATGGGGTCAGTTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGAGCTCACCCTTACCAAGGGTGCGCTCTAc                                                                                                                                 <  1:65954/150‑1 (MQ=255)

              aCTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTAc                                                                                                                                 <  1:438331/150‑1 (MQ=255)

              aCTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTATTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTAc                                                                                                                                 <  1:937930/150‑1 (MQ=255)

              aCTTCTGAGTGCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTAc                                                                                                                                 <  2:1507690/150‑1 (MQ=255)

                  ccgatctCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAAc                                                                                                                             <  1:1635387/144‑1 (MQ=255)

                  cTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAAc                                                                                                                             >  1:720432/1‑150 (MQ=255)

                  cTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAAc                                                                                                                             <  1:1396924/150‑1 (MQ=255)

                      gTTCGGCATGGGGTCAGGTGGGACCCCCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACATTAACCTAAAAAGAGGTGCTGATACCCAGAGTCTAACTGGTGACCTCACCCTTACCAAGGGTGAGCTCTACCAACTGac                                                                                                                         >  1:1670004/1‑149 (MQ=38)

                         cGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCGGAACTGTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGGCCTCACGCTTAGCAAGGGTGCGCTCTGCGAACAGatcgg                                                                                                                      >  2:1635387/1‑146 (MQ=37)

                              tGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCa                                                                                                                 <  2:61740/150‑1 (MQ=255)

                                tagcgcggtggtCCCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGc                                                                                                               >  2:1682149/7‑150 (MQ=255)

                                gggTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCACCTGAGCCATATCAGc                                                                                                               >  2:909388/1‑150 (MQ=255)

                                gggTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGc                                                                                                               <  2:1185516/150‑1 (MQ=255)

                                  gTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCAc                                                                                                             <  2:670090/150‑1 (MQ=255)

                                   gcAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACg                                                                                                            <  1:940860/149‑1 (MQ=255)

                                   gcAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACg                                                                                                            <  2:1725451/149‑1 (MQ=255)

                                            accaccGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTg                                                                                                   <  2:1629930/150‑1 (MQ=255)

                                             ccaccGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGa                                                                                                  <  1:1037663/150‑1 (MQ=255)

                                             ccaccGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGa                                                                                                  <  1:127522/150‑1 (MQ=255)

                                              caccGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGAt                                                                                                 <  1:1353646/150‑1 (MQ=255)

                                                  gcgcTACTGCCGCCAGACAAATTCTTTTCTAATATGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGAACTCCCCCTTACCAAGGGTGCGCTCTACCAACAGAGCCATATCAGCAAGCTAAATTTTATGCCt                                                                                             >  2:1333182/1‑150 (MQ=37)

                                                   ggcTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTg                                                                                            <  1:582659/149‑1 (MQ=255)

                                                     cTACTTCCGCCAGACAAATTCTTTTCTAATCTGCCGTACTTTAACCTAAAAAGTGGTGCTGATACCCAGATTCGAACTTGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGc                                                                                          <  2:1470463/150‑1 (MQ=255)

                                                     cTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGc                                                                                          >  2:1703119/1‑150 (MQ=255)

                                                      tACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCa                                                                                         <  1:655156/150‑1 (MQ=255)

                                                       aCTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAg                                                                                        <  2:1358532/150‑1 (MQ=255)

                                                       aCTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAg                                                                                        <  1:836784/150‑1 (MQ=255)

                                                          gccgccAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTc                                                                                     >  2:541219/1‑150 (MQ=255)

                                                          gccgccAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTc                                                                                     >  1:143296/1‑150 (MQ=255)

                                                          gccgccAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTAACAAGGGAGCGCTCTACAAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTc                                                                                     >  1:1393797/1‑150 (MQ=255)

                                                           ccgccAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTcc                                                                                    >  2:493143/1‑150 (MQ=255)

                                                             gccAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTATTTATTTTTTTTGATAGCCAGAGTCGAACTGGTTAACTAAAATTTACCAATGGTGATCTCTACGATCTTAGCATAATCATTTCGCTATATTTGATGCCTGGCAGTTCCCt                                                                                  <  2:334470/150‑1 (MQ=255)

                                                               cAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTAc                                                                                <  2:720432/150‑1 (MQ=255)

                                                               cAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTAc                                                                                >  2:1657662/1‑150 (MQ=255)

                                                               cAGACAAATTCCTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTAc                                                                                >  1:1128221/1‑150 (MQ=255)

                                                                    aaaTTCTTTTCCAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCCACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACACTCg                                                                           >  1:844147/1‑150 (MQ=255)

                                                                      aTTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCCCGCTAAATTTGATGCCTGGCACTTCCCTACTCTCGCa                                                                         >  2:346706/1‑150 (MQ=255)

                                                                       ttCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCAt                                                                        >  2:1711765/1‑150 (MQ=255)

                                                                         cTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCCCCCTTACCAAGGGTGCGATCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATgg                                                                      >  2:828935/1‑150 (MQ=255)

                                                                             tCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGgag                                                                  >  2:1534571/1‑150 (MQ=255)

                                                                                aaTCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAcc                                                               >  2:1134734/1‑150 (MQ=255)

                                                                                 aTCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAccc                                                              >  1:1695972/1‑150 (MQ=255)

                                                                                  tCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAcccc                                                             <  1:1476347/150‑1 (MQ=255)

                                                                                       cGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACt                                                        <  2:143296/150‑1 (MQ=255)

                                                                                           cTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTcccg                                                    >  2:658746/1‑149 (MQ=255)

                                                                                              tAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTCCCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTGCCCGACTCTCGCATGGGGAGACACCACACTACCATCg                                                 >  1:863490/1‑150 (MQ=255)

                                                                                                aCCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGgc                                               >  1:1128043/1‑150 (MQ=255)

                                                                                                aCCTAAAAAGTGGTGCTGATACCAAGAGTCGAACTTGGGACATAAAAATTACCAAGGGTGAGCTCAACCAACATAGCCATATAAGAACGCTAAATTTGATGCCTGGCAGTTCCCTACTCACGCATGGGTAGACCCAAAACTACCATCGgc                                               >  2:1544002/1‑150 (MQ=21)

                                                                                                  cTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGgcgc                                             >  2:1250257/1‑150 (MQ=255)

                                                                                                   tAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCCCACTACCATCGGCGCt                                            >  2:1365557/1‑150 (MQ=255)

                                                                                                    aaaaaGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTCCCATCGGCGCTa                                           >  1:1422113/1‑150 (MQ=255)

                                                                                                        aGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGc                                       >  2:2150/1‑150 (MQ=255)

                                                                                                           ggtgCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGCGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGtt                                    >  1:1094776/1‑150 (MQ=255)

                                                                                                           ggtgCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCCTCGGCGCTACTGCttt                                    >  1:229293/1‑150 (MQ=255)

                                                                                                           ggtgCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGtt                                    >  1:817837/1‑150 (MQ=255)

                                                                                                              gCTGATACCCAGACTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGATCCATATCAGCACGCTAAATTTGATGCCTTTAAGTTCTCTACTCTCGCATGGGGATACCCAACACTACCATCGGCGCTACGGCGTTTCa                                 <  2:814858/150‑1 (MQ=37)

                                                                                                               cTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCAc                                >  1:1625556/1‑150 (MQ=255)

                                                                                                               cTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCAc                                >  1:768965/1‑150 (MQ=255)

                                                                                                                tGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACt                               <  2:1128221/150‑1 (MQ=255)

                                                                                                                tGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACt                               >  1:614287/1‑150 (MQ=255)

                                                                                                                     cccAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGa                          >  1:415576/1‑150 (MQ=255)

                                                                                                                     cccAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGACATATCAGCACGAGAAATTTGATGCCTGGCAGTTCCCTCCTCTCGCATGGGGAGACCCCACCCAACCATCGGCGCTACGGGGGTTCACGTCTgg                          >  1:940752/1‑149 (MQ=25)

                                                                                                                              gAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAt                 >  2:809628/1‑150 (MQ=255)

                                                                                                                               aaCTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTGCCATCGGCGCTCCGGCGTTTCAGTTCTGAGTTCGGCATg                >  1:506567/1‑150 (MQ=255)

                                                                                                                                aCTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATgg               >  2:795230/1‑150 (MQ=255)

                                                                                                                                aCTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATgg               <  2:1416298/150‑1 (MQ=255)

                                                                                                                                  tGGGGACCTCTCCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTTTTGTTTGGTTGTTCCGTTATCTCGGATTGGGAGACCTCTATATACTATATGCGTTAAGGAGTTTTTCTTATGTGTTCGGCATgggg             <  2:1670004/150‑1 (MQ=12)

                                                                                                                                  tGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATgggg             <  1:909388/150‑1 (MQ=255)

                                                                                                                                     ggACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGAAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCa          >  1:283956/1‑150 (MQ=255)

                                                                                                                                      gACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAg         >  1:1618764/1‑150 (MQ=255)

                                                                                                                                       agcgcACCCTTGGTAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAgg        >  2:370321/5‑150 (MQ=255)

                                                                                                                                        gcgcACCCTTGGTAAGGGTGCGCTCTACCAACTGAGCCCTATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGt       >  1:1596059/4‑150 (MQ=18)

                                                                                                                                        gcgcACCCTTGGTAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGt       >  1:1651121/4‑150 (MQ=255)

                                                                                                                                        ccTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCCGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGt       <  1:1682149/150‑1 (MQ=255)

                                                                                                                                        ccTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGt       >  2:1704014/1‑150 (MQ=255)

                                                                                                                                        ccTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGGTCGGCATGGGGTCAGGt       >  1:475603/1‑150 (MQ=255)

                                                                                                                                        ccTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATAAGCACGCTAAATTTGATGCCTGGCAGTTCCCAACTCTCGCATGGGGAGACACCACACTACCATCGGCGCTAAGGCGTTTCACATCTGAGTTCGGCATGGGGTCAGGt       >  1:369867/1‑150 (MQ=21)

                                                                                                                                         cTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTg      >  2:912717/1‑150 (MQ=255)

                                                                                                                                           cACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCCCACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTggg    >  2:1104531/1‑150 (MQ=255)

                                                                                                                                           cACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCCTCGGCGCTACGGCGTTTCCCTTCTGAGTTCGGCATGGGGTCAGGTggg    >  2:328355/1‑150 (MQ=255)

                                                                                                                                           cACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTggg    >  2:1381108/1‑150 (MQ=255)

                                                                                                                                            aCCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGa   >  1:225423/1‑150 (MQ=255)

                                                                                                                                             cccTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGt            >  1:51906/1‑140 (MQ=255)

                                                                                                                                             cccTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGt            <  2:51906/140‑1 (MQ=255)

                                                                                                                                             cccTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGac  <  1:493143/150‑1 (MQ=255)

                                                                                                                                             cccTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCGCGCAGGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGac  <  1:346706/150‑1 (MQ=255)

CGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGAC  >  NC_000913/3423474‑3423764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`